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Protein

Aryl hydrocarbon receptor

Gene

Ahr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).9 Publications

Miscellaneous

Although it interacts with NEDD8, it does not seem to be neddylated.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Receptor, Repressor
Biological processBiological rhythms, Cell cycle, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Aryl hydrocarbon receptor
Short name:
Ah receptor
Short name:
AhR
Gene namesi
Name:Ahr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105043 Ahr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39R → D: Drastically reduces the binding affinity of AHR?ARNT to dioxin response element (DRE). Impairs ligand-induced nuclear translocation of AHR. 1 Publication1
Mutagenesisi42L → E: Significantly reduces binding affinities of the AHR?ARNT heterodimer to DRE. 1 Publication1
Mutagenesisi43N → A: Reduces the binding affinity of AHR?ARNT to the DRE by more than 10-fold. 1 Publication1
Mutagenesisi49L → E: Reduces the AHR induction transcription activity by 50%. Increases ligand-induced nuclear translocation of AHR. 1 Publication1
Mutagenesisi65K → E: Reduces the binding affinity of AHR?ARNT to the DRE by more than 10-fold. 1 Publication1
Mutagenesisi70R → D: Drastically reduces the AHR transcription activity inductiona. Increases constitutive AHR nuclear translocation in the absence of ligands. Reduces binding affinity for the DRE by more than 30-fold in vitro. 1 Publication1
Mutagenesisi112E → A: Reduces transcription activity. Decreases interaction with ARNT. 1 Publication1
Mutagenesisi115F → A: Highly disrupts the dimerization ability of AHR. 1 Publication1
Mutagenesisi115F → D: Highly disrupts the dimerization ability of AHR. Reduces the AHR transcription factor activity induction by 50%. 2 Publications1
Mutagenesisi116L → E: Highly disrupts the dimerization ability of AHR. Reduces transcription activity. Decreases interaction with ARNT. 1 Publication1
Mutagenesisi119A → D: Highly disrupts the dimerization ability of AHR. Reduces transcription activity. Impairs interaction with ARNT. 1 Publication1
Mutagenesisi120L → D: Significantly reduces binding affinities of the AHR?ARNT heterodimer to DRE. 1 Publication1
Mutagenesisi120L → E: Highly disrupts the dimerization ability of AHR. Reduces transcription activity. Impairs interaction with ARNT. 1 Publication1
Mutagenesisi124V → D: Highly disrupts the dimerization ability of AHR. Reduces transcription activity. Impairs interaction with ARNT. 1 Publication1
Mutagenesisi134F → D: Reduces the AHR induction activity by ?50%. Translocate to thee nucleus at a high level. 1 Publication1
Mutagenesisi152I → D: Completely abolished the AHR induction activity, the ligand-induced nuclear translocation of AHR, and drastically reduced DRE-binding in vitro. 1 Publication1
Mutagenesisi238K → D: Significantly reduces binding affinities of the AHR?ARNT heterodimer to DRE. 1 Publication1
Mutagenesisi240L → D: Significantly reduces binding affinities of the AHR?ARNT heterodimer to DRE. 1 Publication1
Mutagenesisi260F → D: Reduces transcription activity. Impairs interaction with ARNT. 1 Publication1
Mutagenesisi262I → D: Reduces transcription activity. Decreases interaction with ARNT. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL6099
GuidetoPHARMACOLOGYi2951

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000134521 – 92 Publications9
ChainiPRO_000001345310 – 848Aryl hydrocarbon receptorAdd BLAST839

Proteomic databases

MaxQBiP30561
PaxDbiP30561
PRIDEiP30561

PTM databases

iPTMnetiP30561
PhosphoSitePlusiP30561

Expressioni

Tissue specificityi

Expressed in all tissues tested including brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen and thymus.1 Publication

Developmental stagei

Between E10 and E12, abundantly expressed in neuroepithelium, branchial arches, cranial nerves, liver, heart and spinal ganglia.1 Publication

Inductioni

Induced or repressed by TGF-beta and dioxin in a cell-type specific fashion. Repressed by cAMP, retinoic acid, and TPA.1 Publication

Interactioni

Subunit structurei

Homodimer (PubMed:24001774). Interacts with IVNS1ABP (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. Interacts with MYBBP1A and NEDD8. Interacts with ARNTL/BMAL1. Interacts with HSP90AB1 (PubMed:15581363). Interacts with ARNT; the heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:24001774, PubMed:28396409).By similarity6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198037, 8 interactors
CORUMiP30561
DIPiDIP-222N
IntActiP30561, 7 interactors
STRINGi10090.ENSMUSP00000112137

Chemistry databases

BindingDBiP30561

Structurei

Secondary structure

1848
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP30561
SMRiP30561
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 79bHLHPROSITE-ProRule annotationAdd BLAST54
Domaini116 – 179PAS 1PROSITE-ProRule annotationAdd BLAST64
Domaini269 – 336PAS 2PROSITE-ProRule annotationAdd BLAST68
Domaini342 – 380PACAdd BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 65DNA-bindingBy similarityAdd BLAST29
Regioni116 – 124Required for maintaining the overall integrity of the AHR:ARNT heterodimer and its transcriptional activity1 Publication9
Regioni260 – 262Required for maintaining the overall integrity of the AHR:ARNT heterodimer and its transcriptional activity1 Publication3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi594 – 648Gln-richAdd BLAST55

Domaini

The PAS 1 domain is essential for dimerization and also required for AHR:ARNT heterodimerization.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3560 Eukaryota
ENOG410XRZH LUCA
HOGENOMiHOG000252935
HOVERGENiHBG007313
InParanoidiP30561
KOiK09093
PhylomeDBiP30561

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR033348 AHR
IPR039091 AHR/AHRR
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR001610 PAC
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR013655 PAS_fold_3
PANTHERiPTHR10649 PTHR10649, 1 hit
PTHR10649:SF9 PTHR10649:SF9, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PF08447 PAS_3, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00086 PAC, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P30561-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSGANITYA SRKRRKPVQK TVKPIPAEGI KSNPSKRHRD RLNTELDRLA
60 70 80 90 100
SLLPFPQDVI NKLDKLSVLR LSVSYLRAKS FFDVALKSTP ADRNGGQDQC
110 120 130 140 150
RAQIRDWQDL QEGEFLLQAL NGFVLVVTAD ALVFYASSTI QDYLGFQQSD
160 170 180 190 200
VIHQSVYELI HTEDRAEFQR QLHWALNPDS AQGVDEAHGP PQAAVYYTPD
210 220 230 240 250
QLPPENASFM ERCFRCRLRC LLDNSSGFLA MNFQGRLKYL HGQNKKGKDG
260 270 280 290 300
ALLPPQLALF AIATPLQPPS ILEIRTKNFI FRTKHKLDFT PIGCDAKGQL
310 320 330 340 350
ILGYTEVELC TRGSGYQFIH AADMLHCAES HIRMIKTGES GMTVFRLFAK
360 370 380 390 400
HSRWRWVQSN ARLIYRNGRP DYIIATQRPL TDEEGREHLQ KRSTSLPFMF
410 420 430 440 450
ATGEAVLYEI SSPFSPIMDP LPIRTKSNTS RKDWAPQSTP SKDSFHPSSL
460 470 480 490 500
MSALIQQDES IYLCPPSSPA PLDSHFLMGS VSKCGSWQDS FAAAGSEAAL
510 520 530 540 550
KHEQIGHAQD VNLALSGGPS ELFPDNKNND LYNIMRNLGI DFEDIRSMQN
560 570 580 590 600
EEFFRTDSTA AGEVDFKDID ITDEILTYVQ DSLNNSTLMN SACQQQPVTQ
610 620 630 640 650
HLSCMLQERL QLEQQQQLQQ PPPQALEPQQ QLCQMVCPQQ DLGPKHTQIN
660 670 680 690 700
GTFASWNPTP PVSFNCPQQE LKHYQLFSSL QGTAQEFPYK PEVDSVPYTQ
710 720 730 740 750
NFAPCNQPLL PEHSKSVQLD FPGRDFEPSL HPTTSNLDFV SCLQVPENQS
760 770 780 790 800
HGINSQSTMV SPQAYYAGAM SMYQCQPGPQ RTPVDQTQYS SEIPGSQAFL
810 820 830 840
SKVQSRGIFN ETYSSDLSSI GHAAQTTGHL HHLAEARPLP DITPGGFL
Length:848
Mass (Da):95,017
Last modified:March 25, 2003 - v3
Checksum:iAA1560FA83DDD03C
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3U5D9Q3U5D9_MOUSE
Aryl hydrocarbon receptor
Ahr
805Annotation score:
A0A0B4J1L1A0A0B4J1L1_MOUSE
Aryl hydrocarbon receptor
Ahr
114Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74S → T (PubMed:1325649).Curated1
Sequence conflicti74S → T (PubMed:8148872).Curated1
Sequence conflicti132 – 133LV → FL (PubMed:1314586).Curated2
Sequence conflicti132 – 133LV → FL (PubMed:8408082).Curated2
Sequence conflicti171 – 172QL → HV (PubMed:1314586).Curated2
Sequence conflicti171 – 172QL → HV (PubMed:8408082).Curated2
Sequence conflicti351H → N in D38416 (PubMed:7961644).Curated1

Polymorphismi

There are four common alleles; AHRB1; AHRB2; AHRB3 and AHRD. The sequence of AHRB2 is shown here.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti324M → I in allele AHRB1; no increase in specific ligand binding. 6 Publications1
Natural varianti348F → L in allele AHRB1 and allele AHRD; no reduction in specific ligand binding. 7 Publications1
Natural varianti375A → V in allele AHRD; reduced specific ligand binding. 5 Publications1
Natural varianti471P → L in allele AHRB1; no increase in specific ligand binding. 6 Publications1
Natural varianti533N → S in allele AHRB1; no increase in specific ligand binding. 6 Publications1
Natural varianti589M → I in allele AHRD. 2 Publications1
Natural varianti589M → L in allele AHRB1; no increase in specific ligand binding. 5 Publications1
Natural varianti758T → A in allele AHRB1 and allele AHRD. 7 Publications1
Natural varianti806 – 848Missing in allele AHRB1. Add BLAST43
Natural varianti808I → V in allele AHRB3. 1 Publication1
Natural varianti821G → D in allele AHRB3. 1 Publication1
Natural varianti824A → V in allele AHRB3. 1 Publication1
Natural varianti843 – 848TPGGFL → SHLVGSCSSHARMKFIQEQD TGTVRVGHQYYFSKTFDSCI in allele AHRB3. 6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38417 mRNA Translation: BAA07469.1
M94623 Unassigned DNA Translation: AAA02896.1
L19749 Unassigned DNA No translation available.
L19750 Unassigned DNA No translation available.
L19751 Unassigned DNA No translation available.
L19752 Unassigned DNA No translation available.
L19753 Unassigned DNA No translation available.
L19754 Unassigned DNA No translation available.
L19755 Unassigned DNA No translation available.
L19756 Unassigned DNA No translation available.
L19757 Unassigned DNA No translation available.
L19758 Unassigned DNA No translation available.
L19759 Unassigned DNA No translation available.
D38416 mRNA No translation available.
AF325111 Genomic DNA Translation: AAK13443.1
AF405560 mRNA Translation: AAL89725.1
AF405561 mRNA Translation: AAL89726.1
AF405562 mRNA Translation: AAL89727.1
AF405563 mRNA Translation: AAL89728.1
AF405566 mRNA Translation: AAL89731.1
AF405567 mRNA Translation: AAL89732.1
AF405570 mRNA Translation: AAL89735.1
PIRiA46266
RefSeqiNP_038492.1, NM_013464.4
UniGeneiMm.341377

Genome annotation databases

GeneIDi11622
KEGGimmu:11622

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38417 mRNA Translation: BAA07469.1
M94623 Unassigned DNA Translation: AAA02896.1
L19749 Unassigned DNA No translation available.
L19750 Unassigned DNA No translation available.
L19751 Unassigned DNA No translation available.
L19752 Unassigned DNA No translation available.
L19753 Unassigned DNA No translation available.
L19754 Unassigned DNA No translation available.
L19755 Unassigned DNA No translation available.
L19756 Unassigned DNA No translation available.
L19757 Unassigned DNA No translation available.
L19758 Unassigned DNA No translation available.
L19759 Unassigned DNA No translation available.
D38416 mRNA No translation available.
AF325111 Genomic DNA Translation: AAK13443.1
AF405560 mRNA Translation: AAL89725.1
AF405561 mRNA Translation: AAL89726.1
AF405562 mRNA Translation: AAL89727.1
AF405563 mRNA Translation: AAL89728.1
AF405566 mRNA Translation: AAL89731.1
AF405567 mRNA Translation: AAL89732.1
AF405570 mRNA Translation: AAL89735.1
PIRiA46266
RefSeqiNP_038492.1, NM_013464.4
UniGeneiMm.341377

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M4XX-ray2.55A/B110-267[»]
5V0LX-ray4.00B29-267[»]
ProteinModelPortaliP30561
SMRiP30561
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198037, 8 interactors
CORUMiP30561
DIPiDIP-222N
IntActiP30561, 7 interactors
STRINGi10090.ENSMUSP00000112137

Chemistry databases

BindingDBiP30561
ChEMBLiCHEMBL6099
GuidetoPHARMACOLOGYi2951

PTM databases

iPTMnetiP30561
PhosphoSitePlusiP30561

Proteomic databases

MaxQBiP30561
PaxDbiP30561
PRIDEiP30561

Protocols and materials databases

DNASUi11622
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11622
KEGGimmu:11622

Organism-specific databases

CTDi196
MGIiMGI:105043 Ahr

Phylogenomic databases

eggNOGiKOG3560 Eukaryota
ENOG410XRZH LUCA
HOGENOMiHOG000252935
HOVERGENiHBG007313
InParanoidiP30561
KOiK09093
PhylomeDBiP30561

Miscellaneous databases

ChiTaRSiAhr mouse
PROiPR:P30561
SOURCEiSearch...

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR033348 AHR
IPR039091 AHR/AHRR
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR001610 PAC
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR013655 PAS_fold_3
PANTHERiPTHR10649 PTHR10649, 1 hit
PTHR10649:SF9 PTHR10649:SF9, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PF08447 PAS_3, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00086 PAC, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAHR_MOUSE
AccessioniPrimary (citable) accession number: P30561
Secondary accession number(s): Q8QZX6
, Q8R4S3, Q99P79, Q9QVY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 25, 2003
Last modified: November 7, 2018
This is version 184 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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