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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Temperature dependencei

Optimum temperature is about 55 degrees Celsius.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei309NucleophileBy similarity1
Active sitei352Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BRENDAi2.4.1.18 623
UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001886821 – 6391,4-alpha-glucan branching enzyme GlgBAdd BLAST639

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP30538
SMRiP30538
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

P30538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAVGPTDLE IYLFHEGSLY KSYELFGAHV IKKNGMVGTR FCVWAPHARE
60 70 80 90 100
VRLVGSFNEW NGTNFNLMKV SNQGVWMIFI PENLEGHLYK YEITTNDGNV
110 120 130 140 150
LLKSDPYAFY SELRPHTASI VYNIKGYQWN DQTWRRKKQR KRIYDQPLFI
160 170 180 190 200
YELHFGSWKK KEDGSFYTYQ EMAEELIPYV LEHGFTHIEL LPLVEHPFDR
210 220 230 240 250
SWGYQGIGYY SATSRYGTPH DLMYFIDRCH QAGIGVILDW VPGHFCKDSH
260 270 280 290 300
GLYMFDGAPA YEYANMQDRE NYVWGTANFD LGKPEVRSFL ISNALFWMEY
310 320 330 340 350
FHVDGFRVDA VANMLYWPNS DVLYKNTYAV EFLQKLNETV FAYDPNILMI
360 370 380 390 400
AEDSTDWPRV TAPTYDGGLG FNYKWNMGWM NDILTYMETP PEHRKYVHNK
410 420 430 440 450
VTFSLLYAYS ENFILPFSHD EVVHGKKSLL SKMPGTYEEK FAQLRLLYGY
460 470 480 490 500
LLTHPGKKLL FMGGEFGQFD EWKDLEQLDW MLFDFDMHRN MNMYVKELLK
510 520 530 540 550
CYKRYKPLYE LDHSPDGFEW IDVHNAEQSI FSFIRRGKKE DDLLIVVCNF
560 570 580 590 600
TNKVYHGYKV GVPLFTRYRE VINSDAIQFG GFGNINPKPI AAMEGPFHGK
610 620 630
PYHIQMTIPP FGISILRPVK KGSVKSFMKT PHPPSHGAS
Length:639
Mass (Da):74,795
Last modified:April 1, 1993 - v1
Checksum:iF5BD4446B371E03A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35089 Genomic DNA Translation: AAA22482.1
PIRiS18599

Similar proteinsi

Entry informationi

Entry nameiGLGB_GEOSE
AccessioniPrimary (citable) accession number: P30538
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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