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UniProtKB - P30532 (ACHA5_HUMAN)
Protein
Neuronal acetylcholine receptor subunit alpha-5
Gene
CHRNA5
Organism
Homo sapiens (Human)
Status
Functioni
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
GO - Molecular functioni
- acetylcholine-gated cation-selective channel activity Source: DFLAT
- acetylcholine receptor activity Source: UniProtKB
- ligand-gated ion channel activity Source: DFLAT
- neurotransmitter receptor activity Source: GO_Central
GO - Biological processi
- behavioral response to nicotine Source: UniProtKB
- chemical synaptic transmission Source: GO_Central
- ion transmembrane transport Source: GO_Central
- membrane depolarization Source: ComplexPortal
- nervous system process Source: GO_Central
- regulation of membrane potential Source: GO_Central
- regulation of synaptic vesicle exocytosis Source: Ensembl
- response to nicotine Source: GO_Central
- signal transduction Source: GO_Central
- synaptic transmission, cholinergic Source: GO_Central
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P30532 |
Reactomei | R-HSA-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-HSA-629597, Highly calcium permeable nicotinic acetylcholine receptors |
SignaLinki | P30532 |
Names & Taxonomyi
Protein namesi | Recommended name: Neuronal acetylcholine receptor subunit alpha-5 |
Gene namesi | Name:CHRNA5 Synonyms:NACHRA5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1959, CHRNA5 |
MIMi | 118505, gene |
neXtProti | NX_P30532 |
VEuPathDBi | HostDB:ENSG00000169684 |
Subcellular locationi
Plasma Membrane
- acetylcholine-gated channel complex Source: ComplexPortal
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: Reactome
- postsynaptic membrane Source: UniProtKB-SubCell
Other locations
- dopaminergic synapse Source: Ensembl
- neuron projection Source: GO_Central
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 23 – 254 | ExtracellularSequence analysisAdd BLAST | 232 | |
Transmembranei | 255 – 275 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 282 – 302 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 317 – 337 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 338 – 429 | CytoplasmicSequence analysisAdd BLAST | 92 | |
Transmembranei | 430 – 451 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 452 – 468 | ExtracellularSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Organism-specific databases
DisGeNETi | 1138 |
MalaCardsi | CHRNA5 |
MIMi | 612052, phenotype |
OpenTargetsi | ENSG00000169684 |
PharmGKBi | PA26491 |
Miscellaneous databases
Pharosi | P30532, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3038461 CHEMBL3137272 CHEMBL3137273 |
DrugBanki | DB00237, Butabarbital DB00898, Ethanol DB00184, Nicotine |
Genetic variation databases
BioMutai | CHRNA5 |
DMDMi | 2506126 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000000356 | 23 – 468 | Neuronal acetylcholine receptor subunit alpha-5Add BLAST | 446 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 155 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 170 ↔ 184 | By similarity | ||
Glycosylationi | 183 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 229 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 234 ↔ 235 | Associated with receptor activationBy similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | P30532 |
MaxQBi | P30532 |
PaxDbi | P30532 |
PeptideAtlasi | P30532 |
PRIDEi | P30532 |
ProteomicsDBi | 54715 |
PTM databases
GlyGeni | P30532, 3 sites |
iPTMneti | P30532 |
PhosphoSitePlusi | P30532 |
Expressioni
Gene expression databases
Bgeei | ENSG00000169684, Expressed in subventricular zone (outer) (primate) and 121 other tissues |
ExpressionAtlasi | P30532, baseline and differential |
Genevisiblei | P30532, HS |
Organism-specific databases
HPAi | ENSG00000169684, Low tissue specificity |
Interactioni
Subunit structurei
Neuronal AChR seems to be composed of two different type of subunits: alpha and non-alpha (beta).
Interacts with LYPD6 (PubMed:27344019).
1 PublicationBinary interactionsi
P30532
With | #Exp. | IntAct |
---|---|---|
CHRNB2 [P17787] | 3 | EBI-6657490,EBI-9008612 |
Protein-protein interaction databases
BioGRIDi | 107560, 52 interactors |
ComplexPortali | CPX-187, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta2 CPX-210, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4 CPX-218, Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2 |
IntActi | P30532, 9 interactors |
STRINGi | 9606.ENSP00000299565 |
Chemistry databases
BindingDBi | P30532 |
Miscellaneous databases
RNActi | P30532, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily. [View classification]Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000159270 |
HOGENOMi | CLU_018074_1_2_1 |
InParanoidi | P30532 |
OMAi | KWANIII |
OrthoDBi | 381858at2759 |
PhylomeDBi | P30532 |
TreeFami | TF315605 |
Family and domain databases
Gene3Di | 1.20.58.390, 2 hits 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR038050, Neuro_actylchol_rec IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 2 hits |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P30532-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAARGSGPRA LRLLLLVQLV AGRCGLAGAA GGAQRGLSEP SSIAKHEDSL
60 70 80 90 100
LKDLFQDYER WVRPVEHLND KIKIKFGLAI SQLVDVDEKN QLMTTNVWLK
110 120 130 140 150
QEWIDVKLRW NPDDYGGIKV IRVPSDSVWT PDIVLFDNAD GRFEGTSTKT
160 170 180 190 200
VIRYNGTVTW TPPANYKSSC TIDVTFFPFD LQNCSMKFGS WTYDGSQVDI
210 220 230 240 250
ILEDQDVDKR DFFDNGEWEI VSATGSKGNR TDSCCWYPYV TYSFVIKRLP
260 270 280 290 300
LFYTLFLIIP CIGLSFLTVL VFYLPSNEGE KICLCTSVLV SLTVFLLVIE
310 320 330 340 350
EIIPSSSKVI PLIGEYLVFT MIFVTLSIMV TVFAINIHHR SSSTHNAMAP
360 370 380 390 400
LVRKIFLHTL PKLLCMRSHV DRYFTQKEET ESGSGPKSSR NTLEAALDSI
410 420 430 440 450
RYITRHIMKE NDVREVVEDW KFIAQVLDRM FLWTFLFVSI VGSLGLFVPV
460
IYKWANILIP VHIGNANK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YM98 | H0YM98_HUMAN | Cholinergic receptor, nicotinic, al... | CHRNA5 hCG_95858 | 160 | Annotation score: | ||
H7BYM0 | H7BYM0_HUMAN | Neuronal acetylcholine receptor sub... | CHRNA5 | 182 | Annotation score: | ||
H0YK34 | H0YK34_HUMAN | Neuronal acetylcholine receptor sub... | CHRNA5 | 56 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 23 – 34 | RCGLA…AGGAQ → ALRSSRARRAAR in AAA58357 (PubMed:1542648).CuratedAdd BLAST | 12 | |
Sequence conflicti | 128 | V → S in AAA58357 (PubMed:1542648).Curated | 1 | |
Sequence conflicti | 365 | C → S in AAA58357 (PubMed:1542648).Curated | 1 | |
Sequence conflicti | 405 | R → T in AAA58357 (PubMed:1542648).Curated | 1 |
Polymorphismi
Genetic variations in CHRNA5 have been associated with susceptibility to smoking-related behavioral traits and lung cancer, contributing to the smoking quantitative trait locus 3 (SQTL3) [MIMi:612052].
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_046211 | 134 | V → I. Corresponds to variant dbSNP:rs2229961Ensembl. | 1 | |
Natural variantiVAR_046212 | 398 | D → N Associated with susceptibility to lung cancer. 2 PublicationsCorresponds to variant dbSNP:rs16969968Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83712 mRNA Translation: AAA58357.1 U62434 mRNA Translation: AAB40112.1 Y08419 mRNA Translation: CAA69696.1 AJ306481 , AJ306482, AJ306483, AJ306484, AJ306485, AJ306486 Genomic DNA Translation: CAC34820.1 BC033639 mRNA Translation: AAH33639.1 |
CCDSi | CCDS10304.1 |
PIRi | A38223 |
RefSeqi | NP_000736.2, NM_000745.3 |
Genome annotation databases
Ensembli | ENST00000299565; ENSP00000299565; ENSG00000169684 |
GeneIDi | 1138 |
KEGGi | hsa:1138 |
UCSCi | uc002bdy.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83712 mRNA Translation: AAA58357.1 U62434 mRNA Translation: AAB40112.1 Y08419 mRNA Translation: CAA69696.1 AJ306481 , AJ306482, AJ306483, AJ306484, AJ306485, AJ306486 Genomic DNA Translation: CAC34820.1 BC033639 mRNA Translation: AAH33639.1 |
CCDSi | CCDS10304.1 |
PIRi | A38223 |
RefSeqi | NP_000736.2, NM_000745.3 |
3D structure databases
SMRi | P30532 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 107560, 52 interactors |
ComplexPortali | CPX-187, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta2 CPX-210, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4 CPX-218, Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2 |
IntActi | P30532, 9 interactors |
STRINGi | 9606.ENSP00000299565 |
Chemistry databases
BindingDBi | P30532 |
ChEMBLi | CHEMBL3038461 CHEMBL3137272 CHEMBL3137273 |
DrugBanki | DB00237, Butabarbital DB00898, Ethanol DB00184, Nicotine |
PTM databases
GlyGeni | P30532, 3 sites |
iPTMneti | P30532 |
PhosphoSitePlusi | P30532 |
Genetic variation databases
BioMutai | CHRNA5 |
DMDMi | 2506126 |
Proteomic databases
MassIVEi | P30532 |
MaxQBi | P30532 |
PaxDbi | P30532 |
PeptideAtlasi | P30532 |
PRIDEi | P30532 |
ProteomicsDBi | 54715 |
Protocols and materials databases
Antibodypediai | 15098, 334 antibodies from 36 providers |
DNASUi | 1138 |
Genome annotation databases
Ensembli | ENST00000299565; ENSP00000299565; ENSG00000169684 |
GeneIDi | 1138 |
KEGGi | hsa:1138 |
UCSCi | uc002bdy.3, human |
Organism-specific databases
CTDi | 1138 |
DisGeNETi | 1138 |
GeneCardsi | CHRNA5 |
HGNCi | HGNC:1959, CHRNA5 |
HPAi | ENSG00000169684, Low tissue specificity |
MalaCardsi | CHRNA5 |
MIMi | 118505, gene 612052, phenotype |
neXtProti | NX_P30532 |
OpenTargetsi | ENSG00000169684 |
PharmGKBi | PA26491 |
VEuPathDBi | HostDB:ENSG00000169684 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000159270 |
HOGENOMi | CLU_018074_1_2_1 |
InParanoidi | P30532 |
OMAi | KWANIII |
OrthoDBi | 381858at2759 |
PhylomeDBi | P30532 |
TreeFami | TF315605 |
Enzyme and pathway databases
PathwayCommonsi | P30532 |
Reactomei | R-HSA-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-HSA-629597, Highly calcium permeable nicotinic acetylcholine receptors |
SignaLinki | P30532 |
Miscellaneous databases
BioGRID-ORCSi | 1138, 2 hits in 1040 CRISPR screens |
GeneWikii | CHRNA5 |
GenomeRNAii | 1138 |
Pharosi | P30532, Tbio |
PROi | PR:P30532 |
RNActi | P30532, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169684, Expressed in subventricular zone (outer) (primate) and 121 other tissues |
ExpressionAtlasi | P30532, baseline and differential |
Genevisiblei | P30532, HS |
Family and domain databases
Gene3Di | 1.20.58.390, 2 hits 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR038050, Neuro_actylchol_rec IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 2 hits |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACHA5_HUMAN | |
Accessioni | P30532Primary (citable) accession number: P30532 Secondary accession number(s): Q15824, Q99554 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families