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UniProtKB - P30520 (PURA2_HUMAN)
Protein
Adenylosuccinate synthetase isozyme 2
Gene
ADSS2
Organism
Homo sapiens (Human)
Status
Functioni
Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
By similarityCatalytic activityi
- EC:6.3.4.4UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
Activity regulationi
Inhibited competitively by AMP and IMP and non-competitively by fructose 1,6-bisphosphate.By similarity
: AMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 40 | Proton acceptorUniRule annotation | 1 | |
Metal bindingi | 40 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 40 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 67 | Magnesium; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 68 | Proton donorUniRule annotation | 1 | |
Binding sitei | 162 | IMPUniRule annotation | 1 | |
Binding sitei | 176 | IMP; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 255 | IMPUniRule annotation | 1 | |
Binding sitei | 270 | IMPUniRule annotation | 1 | |
Binding sitei | 334 | IMPUniRule annotation | 1 | |
Binding sitei | 336 | GTPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 39 – 45 | GTP | 7 | |
Nucleotide bindingi | 67 – 69 | GTP | 3 | |
Nucleotide bindingi | 362 – 364 | GTP | 3 | |
Nucleotide bindingi | 444 – 447 | GTP | 4 |
GO - Molecular functioni
- adenylosuccinate synthase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB-UniRule
- phosphate ion binding Source: UniProtKB
GO - Biological processi
- 'de novo' AMP biosynthetic process Source: GO_Central
- AMP biosynthetic process Source: UniProtKB
- aspartate metabolic process Source: Ensembl
- cellular response to electrical stimulus Source: Ensembl
- immune system process Source: UniProtKB
- IMP metabolic process Source: GO_Central
- response to ammonium ion Source: Ensembl
- response to purine-containing compound Source: Ensembl
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P30520 |
Reactomei | R-HSA-73817, Purine ribonucleoside monophosphate biosynthesis |
SignaLinki | P30520 |
UniPathwayi | UPA00075;UER00335 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenylosuccinate synthetase isozyme 2UniRule annotation (EC:6.3.4.4UniRule annotation)Short name: AMPSase 2UniRule annotation Short name: AdSS 2UniRule annotation Alternative name(s): Adenylosuccinate synthetase, acidic isozymeUniRule annotation Adenylosuccinate synthetase, liver isozymeUniRule annotation Short name: L-type adenylosuccinate synthetaseUniRule annotation IMP--aspartate ligase 2UniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:292, ADSS2 |
MIMi | 103060, gene |
neXtProti | NX_P30520 |
VEuPathDBi | HostDB:ENSG00000035687 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Note: Partially associated with particulate fractions.
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, MitochondrionPathology & Biotechi
Organism-specific databases
DisGeNETi | 159 |
OpenTargetsi | ENSG00000035687 |
PharmGKBi | PA24601 |
Miscellaneous databases
Pharosi | P30520, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4875 |
DrugBanki | DB00787, Acyclovir DB05540, Alanosine DB00128, Aspartic acid |
Genetic variation databases
BioMutai | ADSS |
DMDMi | 21264498 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095130 | 1 – 456 | Adenylosuccinate synthetase isozyme 2Add BLAST | 456 |
Proteomic databases
CPTACi | CPTAC-163 CPTAC-164 |
EPDi | P30520 |
jPOSTi | P30520 |
MassIVEi | P30520 |
MaxQBi | P30520 |
PaxDbi | P30520 |
PeptideAtlasi | P30520 |
PRIDEi | P30520 |
ProteomicsDBi | 54711 |
PTM databases
GlyGeni | P30520, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P30520 |
MetOSitei | P30520 |
PhosphoSitePlusi | P30520 |
SwissPalmi | P30520 |
Expressioni
Gene expression databases
Bgeei | ENSG00000035687, Expressed in corpus callosum and 239 other tissues |
ExpressionAtlasi | P30520, baseline and differential |
Genevisiblei | P30520, HS |
Organism-specific databases
HPAi | ENSG00000035687, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationBinary interactionsi
P30520
With | #Exp. | IntAct |
---|---|---|
SMYD1 [Q8NB12] | 4 | EBI-1042898,EBI-8463848 |
Protein-protein interaction databases
BioGRIDi | 106668, 99 interactors |
IntActi | P30520, 15 interactors |
MINTi | P30520 |
STRINGi | 9606.ENSP00000355493 |
Chemistry databases
BindingDBi | P30520 |
Miscellaneous databases
RNActi | P30520, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P30520 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30520 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 24 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 40 – 43 | IMP bindingUniRule annotation | 4 | |
Regioni | 65 – 68 | IMP bindingUniRule annotation | 4 | |
Regioni | 330 – 336 | Substrate bindingUniRule annotation | 7 |
Sequence similaritiesi
Belongs to the adenylosuccinate synthetase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG1355, Eukaryota |
GeneTreei | ENSGT00390000015553 |
HOGENOMi | CLU_029848_3_0_1 |
InParanoidi | P30520 |
OMAi | FHHAKPI |
OrthoDBi | 1276527at2759 |
PhylomeDBi | P30520 |
TreeFami | TF300486 |
Family and domain databases
CDDi | cd03108, AdSS, 1 hit |
Gene3Di | 1.10.300.10, 1 hit 3.40.440.10, 1 hit 3.90.170.10, 1 hit |
HAMAPi | MF_00011, Adenylosucc_synth, 1 hit MF_03127, Adenylosucc_synth_vert_acid, 1 hit |
InterProi | View protein in InterPro IPR018220, Adenylosuccin_syn_GTP-bd IPR033128, Adenylosuccin_syn_Lys_AS IPR042109, Adenylosuccinate_synth_dom1 IPR042110, Adenylosuccinate_synth_dom2 IPR042111, Adenylosuccinate_synth_dom3 IPR001114, Adenylosuccinate_synthetase IPR027529, AdSS_2_vert IPR027417, P-loop_NTPase |
PANTHERi | PTHR11846, PTHR11846, 1 hit |
Pfami | View protein in Pfam PF00709, Adenylsucc_synt, 1 hit |
SMARTi | View protein in SMART SM00788, Adenylsucc_synt, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00184, purA, 1 hit |
PROSITEi | View protein in PROSITE PS01266, ADENYLOSUCCIN_SYN_1, 1 hit PS00513, ADENYLOSUCCIN_SYN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P30520-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAFAETYPAA SSLPNGDCGR PRARPGGNRV TVVLGAQWGD EGKGKVVDLL
60 70 80 90 100
AQDADIVCRC QGGNNAGHTV VVDSVEYDFH LLPSGIINPN VTAFIGNGVV
110 120 130 140 150
IHLPGLFEEA EKNVQKGKGL EGWEKRLIIS DRAHIVFDFH QAADGIQEQQ
160 170 180 190 200
RQEQAGKNLG TTKKGIGPVY SSKAARSGLR MCDLVSDFDG FSERFKVLAN
210 220 230 240 250
QYKSIYPTLE IDIEGELQKL KGYMEKIKPM VRDGVYFLYE ALHGPPKKIL
260 270 280 290 300
VEGANAALLD IDFGTYPFVT SSNCTVGGVC TGLGMPPQNV GEVYGVVKAY
310 320 330 340 350
TTRVGIGAFP TEQDNEIGEL LQTRGREFGV TTGRKRRCGW LDLVLLKYAH
360 370 380 390 400
MINGFTALAL TKLDILDMFT EIKVGVAYKL DGEIIPHIPA NQEVLNKVEV
410 420 430 440 450
QYKTLPGWNT DISNARAFKE LPVNAQNYVR FIEDELQIPV KWIGVGKSRE
SMIQLF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 – 25 | RP → A in CAA47123 (PubMed:1592113).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051881 | 179 | L → F. Corresponds to variant dbSNP:rs12134870Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66503 Genomic DNA Translation: CAA47123.1 AL591594 Genomic DNA No translation available. AL645465 Genomic DNA No translation available. CH471148 Genomic DNA Translation: EAW77102.1 BC012356 mRNA Translation: AAH12356.1 |
CCDSi | CCDS1624.1 |
PIRi | S21166 |
RefSeqi | NP_001117.2, NM_001126.3 |
Genome annotation databases
Ensembli | ENST00000366535; ENSP00000355493; ENSG00000035687 |
GeneIDi | 159 |
KEGGi | hsa:159 |
MANE-Selecti | ENST00000366535.4; ENSP00000355493.3; NM_001126.5; NP_001117.2 |
UCSCi | uc001iaj.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66503 Genomic DNA Translation: CAA47123.1 AL591594 Genomic DNA No translation available. AL645465 Genomic DNA No translation available. CH471148 Genomic DNA Translation: EAW77102.1 BC012356 mRNA Translation: AAH12356.1 |
CCDSi | CCDS1624.1 |
PIRi | S21166 |
RefSeqi | NP_001117.2, NM_001126.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2V40 | X-ray | 1.90 | A | 21-456 | [»] | |
SMRi | P30520 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106668, 99 interactors |
IntActi | P30520, 15 interactors |
MINTi | P30520 |
STRINGi | 9606.ENSP00000355493 |
Chemistry databases
BindingDBi | P30520 |
ChEMBLi | CHEMBL4875 |
DrugBanki | DB00787, Acyclovir DB05540, Alanosine DB00128, Aspartic acid |
PTM databases
GlyGeni | P30520, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P30520 |
MetOSitei | P30520 |
PhosphoSitePlusi | P30520 |
SwissPalmi | P30520 |
Genetic variation databases
BioMutai | ADSS |
DMDMi | 21264498 |
Proteomic databases
CPTACi | CPTAC-163 CPTAC-164 |
EPDi | P30520 |
jPOSTi | P30520 |
MassIVEi | P30520 |
MaxQBi | P30520 |
PaxDbi | P30520 |
PeptideAtlasi | P30520 |
PRIDEi | P30520 |
ProteomicsDBi | 54711 |
Protocols and materials databases
Antibodypediai | 20829, 153 antibodies from 23 providers |
DNASUi | 159 |
Genome annotation databases
Ensembli | ENST00000366535; ENSP00000355493; ENSG00000035687 |
GeneIDi | 159 |
KEGGi | hsa:159 |
MANE-Selecti | ENST00000366535.4; ENSP00000355493.3; NM_001126.5; NP_001117.2 |
UCSCi | uc001iaj.4, human |
Organism-specific databases
CTDi | 159 |
DisGeNETi | 159 |
GeneCardsi | ADSS2 |
HGNCi | HGNC:292, ADSS2 |
HPAi | ENSG00000035687, Low tissue specificity |
MIMi | 103060, gene |
neXtProti | NX_P30520 |
OpenTargetsi | ENSG00000035687 |
PharmGKBi | PA24601 |
VEuPathDBi | HostDB:ENSG00000035687 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1355, Eukaryota |
GeneTreei | ENSGT00390000015553 |
HOGENOMi | CLU_029848_3_0_1 |
InParanoidi | P30520 |
OMAi | FHHAKPI |
OrthoDBi | 1276527at2759 |
PhylomeDBi | P30520 |
TreeFami | TF300486 |
Enzyme and pathway databases
UniPathwayi | UPA00075;UER00335 |
PathwayCommonsi | P30520 |
Reactomei | R-HSA-73817, Purine ribonucleoside monophosphate biosynthesis |
SignaLinki | P30520 |
Miscellaneous databases
BioGRID-ORCSi | 159, 329 hits in 1039 CRISPR screens |
ChiTaRSi | ADSS, human |
EvolutionaryTracei | P30520 |
GenomeRNAii | 159 |
Pharosi | P30520, Tchem |
PROi | PR:P30520 |
RNActi | P30520, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000035687, Expressed in corpus callosum and 239 other tissues |
ExpressionAtlasi | P30520, baseline and differential |
Genevisiblei | P30520, HS |
Family and domain databases
CDDi | cd03108, AdSS, 1 hit |
Gene3Di | 1.10.300.10, 1 hit 3.40.440.10, 1 hit 3.90.170.10, 1 hit |
HAMAPi | MF_00011, Adenylosucc_synth, 1 hit MF_03127, Adenylosucc_synth_vert_acid, 1 hit |
InterProi | View protein in InterPro IPR018220, Adenylosuccin_syn_GTP-bd IPR033128, Adenylosuccin_syn_Lys_AS IPR042109, Adenylosuccinate_synth_dom1 IPR042110, Adenylosuccinate_synth_dom2 IPR042111, Adenylosuccinate_synth_dom3 IPR001114, Adenylosuccinate_synthetase IPR027529, AdSS_2_vert IPR027417, P-loop_NTPase |
PANTHERi | PTHR11846, PTHR11846, 1 hit |
Pfami | View protein in Pfam PF00709, Adenylsucc_synt, 1 hit |
SMARTi | View protein in SMART SM00788, Adenylsucc_synt, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00184, purA, 1 hit |
PROSITEi | View protein in PROSITE PS01266, ADENYLOSUCCIN_SYN_1, 1 hit PS00513, ADENYLOSUCCIN_SYN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PURA2_HUMAN | |
Accessioni | P30520Primary (citable) accession number: P30520 Secondary accession number(s): B1AQM5, Q96EG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | May 27, 2002 | |
Last modified: | February 23, 2022 | |
This is version 208 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families