UniProtKB - P30419 (NMT1_HUMAN)
Protein
Glycylpeptide N-tetradecanoyltransferase 1
Gene
NMT1
Organism
Homo sapiens (Human)
Status
Functioni
Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.4 Publications
Catalytic activityi
- N-terminal glycyl-[protein] + tetradecanoyl-CoA = CoA + H+ + N-tetradecanoylglycyl-[protein]4 PublicationsEC:2.3.1.974 Publications
GO - Molecular functioni
- glycylpeptide N-tetradecanoyltransferase activity Source: UniProtKB
- myristoyltransferase activity Source: BHF-UCL
GO - Biological processi
- cellular ketone metabolic process Source: BHF-UCL
- in utero embryonic development Source: Ensembl
- N-terminal peptidyl-glycine N-myristoylation Source: UniProtKB
- N-terminal protein myristoylation Source: Reactome
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
- regulation of rhodopsin mediated signaling pathway Source: Reactome
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.1.97, 2681 |
PathwayCommonsi | P30419 |
Reactomei | R-HSA-162599, Late Phase of HIV Life Cycle R-HSA-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-HSA-75108, Activation, myristolyation of BID and translocation to mitochondria |
SIGNORi | P30419 |
Chemistry databases
SwissLipidsi | SLP:000001287 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycylpeptide N-tetradecanoyltransferase 1 (EC:2.3.1.974 Publications)Alternative name(s): Myristoyl-CoA:protein N-myristoyltransferase 1 Short name: NMT 1 Short name: Type I N-myristoyltransferase Peptide N-myristoyltransferase 1 |
Gene namesi | Name:NMT1 Synonyms:NMT |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000136448.11 |
HGNCi | HGNC:7857, NMT1 |
MIMi | 160993, gene |
neXtProti | NX_P30419 |
Subcellular locationi
Cytosol
- cytosol 1 Publication
Other locations
- Cytoplasm 2 Publications
- Membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Copurifies with ribosomes.1 Publication
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrion Source: GOC
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: UniProtKB
- extrinsic component of membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 492 | G → D or K: Reduced activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 4836 |
OpenTargetsi | ENSG00000136448 |
PharmGKBi | PA31661 |
Miscellaneous databases
Pharosi | P30419, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2593 |
DrugBanki | DB03062, (1-Methyl-1h-Imidazol-2-Yl)-(3-Methyl-4-{3-[(Pyridin-3-Ylmethyl)-Amino]-Propoxy}-Benzofuran-2-Yl)-Methanone |
Polymorphism and mutation databases
BioMutai | NMT1 |
DMDMi | 12231020 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064221 | 1 – 496 | Glycylpeptide N-tetradecanoyltransferase 1Add BLAST | 496 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 31 | PhosphoserineBy similarity | 1 | |
Modified residuei | 47 | PhosphoserineCombined sources | 1 | |
Modified residuei | 83 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P30419 |
jPOSTi | P30419 |
MassIVEi | P30419 |
MaxQBi | P30419 |
PaxDbi | P30419 |
PeptideAtlasi | P30419 |
PRIDEi | P30419 |
ProteomicsDBi | 54667 [P30419-1] 54668 [P30419-2] |
TopDownProteomicsi | P30419-2 [P30419-2] |
PTM databases
iPTMneti | P30419 |
MetOSitei | P30419 |
PhosphoSitePlusi | P30419 |
SwissPalmi | P30419 |
Expressioni
Tissue specificityi
Heart, gut, kidney, liver and placenta.
Gene expression databases
Bgeei | ENSG00000136448, Expressed in frontal cortex and 239 other tissues |
ExpressionAtlasi | P30419, baseline and differential |
Genevisiblei | P30419, HS |
Organism-specific databases
HPAi | ENSG00000136448, Low tissue specificity |
Interactioni
Binary interactionsi
P30419
With | #Exp. | IntAct |
---|---|---|
ACBD6 [Q9BR61] | 5 | EBI-5280164,EBI-2848793 |
Protein-protein interaction databases
BioGRIDi | 110899, 107 interactors |
IntActi | P30419, 42 interactors |
MINTi | P30419 |
STRINGi | 9606.ENSP00000468424 |
Chemistry databases
BindingDBi | P30419 |
Miscellaneous databases
RNActi | P30419, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P30419 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30419 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 118 – 120 | Myristoyl-CoA binding2 Publications | 3 | |
Regioni | 248 – 250 | Myristoyl-CoA binding2 Publications | 3 | |
Regioni | 256 – 260 | Myristoyl-CoA binding2 Publications | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 55 – 67 | Poly-LysAdd BLAST | 13 |
Sequence similaritiesi
Belongs to the NMT family.Curated
Phylogenomic databases
eggNOGi | KOG2779, Eukaryota |
GeneTreei | ENSGT00390000017837 |
HOGENOMi | CLU_022882_1_0_1 |
InParanoidi | P30419 |
OMAi | SHLARNM |
OrthoDBi | 1025421at2759 |
PhylomeDBi | P30419 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000903, MyristoylCoA_TrFase IPR022677, MyristoylCoA_TrFase_C IPR022678, MyristoylCoA_TrFase_CS IPR022676, MyristoylCoA_TrFase_N |
PANTHERi | PTHR11377, PTHR11377, 1 hit |
Pfami | View protein in Pfam PF01233, NMT, 1 hit PF02799, NMT_C, 1 hit |
PIRSFi | PIRSF015892, N-myristl_transf, 1 hit |
SUPFAMi | SSF55729, SSF55729, 2 hits |
PROSITEi | View protein in PROSITE PS00975, NMT_1, 1 hit PS00976, NMT_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform Long (identifier: P30419-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADESETAVK PPAPPLPQMM EGNGNGHEHC SDCENEEDNS YNRGGLSPAN
60 70 80 90 100
DTGAKKKKKK QKKKKEKGSE TDSAQDQPVK MNSLPAERIQ EIQKAIELFS
110 120 130 140 150
VGQGPAKTME EASKRSYQFW DTQPVPKLGE VVNTHGPVEP DKDNIRQEPY
160 170 180 190 200
TLPQGFTWDA LDLGDRGVLK ELYTLLNENY VEDDDNMFRF DYSPEFLLWA
210 220 230 240 250
LRPPGWLPQW HCGVRVVSSR KLVGFISAIP ANIHIYDTEK KMVEINFLCV
260 270 280 290 300
HKKLRSKRVA PVLIREITRR VHLEGIFQAV YTAGVVLPKP VGTCRYWHRS
310 320 330 340 350
LNPRKLIEVK FSHLSRNMTM QRTMKLYRLP ETPKTAGLRP METKDIPVVH
360 370 380 390 400
QLLTRYLKQF HLTPVMSQEE VEHWFYPQEN IIDTFVVENA NGEVTDFLSF
410 420 430 440 450
YTLPSTIMNH PTHKSLKAAY SFYNVHTQTP LLDLMSDALV LAKMKGFDVF
460 470 480 490
NALDLMENKT FLEKLKFGIG DGNLQYYLYN WKCPSMGAEK VGLVLQ
Isoform Short (identifier: P30419-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-80: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EN82 | K7EN82_HUMAN | Glycylpeptide N-tetradecanoyltransf... | NMT1 | 72 | Annotation score: | ||
K7EN42 | K7EN42_HUMAN | Glycylpeptide N-tetradecanoyltransf... | NMT1 | 88 | Annotation score: | ||
B7Z8J4 | B7Z8J4_HUMAN | Glycylpeptide N-tetradecanoyltransf... | NMT1 | 137 | Annotation score: |
Sequence cautioni
The sequence AAC09294 differs from that shown. Reason: Erroneous initiation.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050286 | 61 | Q → K. Corresponds to variant dbSNP:rs3087878Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003570 | 1 – 80 | Missing in isoform Short. 1 PublicationAdd BLAST | 80 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF043324 mRNA Translation: AAC09294.1 Different initiation. AF020500 mRNA Translation: AAB95316.1 AK291936 mRNA Translation: BAF84625.1 CH471178 Genomic DNA Translation: EAW51554.1 BC006538 mRNA Translation: AAH06538.1 BC006569 mRNA Translation: AAH06569.1 BC007258 mRNA Translation: AAH07258.2 BC008312 mRNA Translation: AAH08312.2 M86707 mRNA No translation available. Y17208 mRNA Translation: CAA76685.1 Y17209 mRNA Translation: CAA76686.1 |
CCDSi | CCDS11494.1 [P30419-1] |
PIRi | JC1343 |
RefSeqi | NP_066565.1, NM_021079.4 [P30419-1] |
Genome annotation databases
Ensembli | ENST00000258960; ENSP00000258960; ENSG00000136448 [P30419-1] ENST00000592782; ENSP00000468424; ENSG00000136448 [P30419-1] |
GeneIDi | 4836 |
KEGGi | hsa:4836 |
UCSCi | uc002ihz.4, human [P30419-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF043324 mRNA Translation: AAC09294.1 Different initiation. AF020500 mRNA Translation: AAB95316.1 AK291936 mRNA Translation: BAF84625.1 CH471178 Genomic DNA Translation: EAW51554.1 BC006538 mRNA Translation: AAH06538.1 BC006569 mRNA Translation: AAH06569.1 BC007258 mRNA Translation: AAH07258.2 BC008312 mRNA Translation: AAH08312.2 M86707 mRNA No translation available. Y17208 mRNA Translation: CAA76685.1 Y17209 mRNA Translation: CAA76686.1 |
CCDSi | CCDS11494.1 [P30419-1] |
PIRi | JC1343 |
RefSeqi | NP_066565.1, NM_021079.4 [P30419-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RXT | X-ray | 3.00 | A/B/C/D | 1-496 | [»] | |
3IU1 | X-ray | 1.42 | A/B | 115-496 | [»] | |
3IU2 | X-ray | 1.73 | A/B | 115-496 | [»] | |
3IWE | X-ray | 1.79 | A/B | 115-496 | [»] | |
3JTK | X-ray | 1.61 | A/B | 115-496 | [»] | |
4C2Y | X-ray | 1.64 | A/B | 109-496 | [»] | |
4C2Z | X-ray | 2.08 | A/B | 109-496 | [»] | |
5MU6 | X-ray | 1.88 | A/B | 109-496 | [»] | |
5NPQ | X-ray | 2.37 | A/B | 109-496 | [»] | |
5O6H | X-ray | 1.29 | A/B | 109-496 | [»] | |
5O6J | X-ray | 1.45 | A/B | 109-496 | [»] | |
5O9S | X-ray | 2.70 | A/B | 99-496 | [»] | |
5O9T | X-ray | 2.15 | A/B | 99-496 | [»] | |
5O9U | X-ray | 1.85 | A/B | 99-496 | [»] | |
5O9V | X-ray | 2.20 | A/B | 99-496 | [»] | |
5UUT | X-ray | 2.25 | A/B | 109-496 | [»] | |
6EHJ | X-ray | 2.10 | A/B | 109-496 | [»] | |
6F56 | X-ray | 1.94 | A/B/C/D | 109-496 | [»] | |
6FZ2 | X-ray | 2.05 | A/B | 115-496 | [»] | |
6FZ3 | X-ray | 2.00 | A/B | 115-496 | [»] | |
6FZ5 | X-ray | 1.89 | A/B | 115-496 | [»] | |
6PAV | X-ray | 2.52 | A/B | 109-496 | [»] | |
6QRM | X-ray | 2.30 | A/B | 99-496 | [»] | |
6SJZ | X-ray | 2.00 | A/B | 99-496 | [»] | |
6SK2 | X-ray | 1.90 | A/B | 99-496 | [»] | |
6SK3 | X-ray | 2.70 | A/B | 99-493 | [»] | |
6SK8 | X-ray | 1.87 | A/B | 99-493 | [»] | |
6SKJ | X-ray | 2.80 | A/B | 99-494 | [»] | |
SMRi | P30419 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110899, 107 interactors |
IntActi | P30419, 42 interactors |
MINTi | P30419 |
STRINGi | 9606.ENSP00000468424 |
Chemistry databases
BindingDBi | P30419 |
ChEMBLi | CHEMBL2593 |
DrugBanki | DB03062, (1-Methyl-1h-Imidazol-2-Yl)-(3-Methyl-4-{3-[(Pyridin-3-Ylmethyl)-Amino]-Propoxy}-Benzofuran-2-Yl)-Methanone |
SwissLipidsi | SLP:000001287 |
PTM databases
iPTMneti | P30419 |
MetOSitei | P30419 |
PhosphoSitePlusi | P30419 |
SwissPalmi | P30419 |
Polymorphism and mutation databases
BioMutai | NMT1 |
DMDMi | 12231020 |
Proteomic databases
EPDi | P30419 |
jPOSTi | P30419 |
MassIVEi | P30419 |
MaxQBi | P30419 |
PaxDbi | P30419 |
PeptideAtlasi | P30419 |
PRIDEi | P30419 |
ProteomicsDBi | 54667 [P30419-1] 54668 [P30419-2] |
TopDownProteomicsi | P30419-2 [P30419-2] |
Protocols and materials databases
Antibodypediai | 17578, 181 antibodies |
DNASUi | 4836 |
Genome annotation databases
Ensembli | ENST00000258960; ENSP00000258960; ENSG00000136448 [P30419-1] ENST00000592782; ENSP00000468424; ENSG00000136448 [P30419-1] |
GeneIDi | 4836 |
KEGGi | hsa:4836 |
UCSCi | uc002ihz.4, human [P30419-1] |
Organism-specific databases
CTDi | 4836 |
DisGeNETi | 4836 |
EuPathDBi | HostDB:ENSG00000136448.11 |
GeneCardsi | NMT1 |
HGNCi | HGNC:7857, NMT1 |
HPAi | ENSG00000136448, Low tissue specificity |
MIMi | 160993, gene |
neXtProti | NX_P30419 |
OpenTargetsi | ENSG00000136448 |
PharmGKBi | PA31661 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2779, Eukaryota |
GeneTreei | ENSGT00390000017837 |
HOGENOMi | CLU_022882_1_0_1 |
InParanoidi | P30419 |
OMAi | SHLARNM |
OrthoDBi | 1025421at2759 |
PhylomeDBi | P30419 |
Enzyme and pathway databases
BRENDAi | 2.3.1.97, 2681 |
PathwayCommonsi | P30419 |
Reactomei | R-HSA-162599, Late Phase of HIV Life Cycle R-HSA-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-HSA-75108, Activation, myristolyation of BID and translocation to mitochondria |
SIGNORi | P30419 |
Miscellaneous databases
BioGRID-ORCSi | 4836, 448 hits in 846 CRISPR screens |
ChiTaRSi | NMT1, human |
EvolutionaryTracei | P30419 |
GeneWikii | N-myristoyltransferase_1 |
GenomeRNAii | 4836 |
Pharosi | P30419, Tchem |
PROi | PR:P30419 |
RNActi | P30419, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000136448, Expressed in frontal cortex and 239 other tissues |
ExpressionAtlasi | P30419, baseline and differential |
Genevisiblei | P30419, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000903, MyristoylCoA_TrFase IPR022677, MyristoylCoA_TrFase_C IPR022678, MyristoylCoA_TrFase_CS IPR022676, MyristoylCoA_TrFase_N |
PANTHERi | PTHR11377, PTHR11377, 1 hit |
Pfami | View protein in Pfam PF01233, NMT, 1 hit PF02799, NMT_C, 1 hit |
PIRSFi | PIRSF015892, N-myristl_transf, 1 hit |
SUPFAMi | SSF55729, SSF55729, 2 hits |
PROSITEi | View protein in PROSITE PS00975, NMT_1, 1 hit PS00976, NMT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NMT1_HUMAN | |
Accessioni | P30419Primary (citable) accession number: P30419 Secondary accession number(s): A8K7C1, Q9UE09 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | January 11, 2001 | |
Last modified: | December 2, 2020 | |
This is version 205 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations