We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P30366 (PP11_ARATH)
Protein
Serine/threonine-protein phosphatase PP1 isozyme 1
Gene
TOPP1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro (PubMed:21222654, PubMed:26943172).
Acts as negative regulator of abscisic acid (ABA) signaling. In vitro, can dephosphorylate and inhibit the kinase activity of SRK2E/SNRK2.6, an activator of ABA signaling.
2 PublicationsCatalytic activityi
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Activity regulationi
Phosphatase activity is strongly reduced by the protein phosphatase inhibitor 2 (I-2) (PubMed:21222654, PubMed:26943172). Phosphatase activity is strongly reduced by PYL11, an abscisic acid (ABA) receptor (PubMed:26943172).2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 77 | Manganese 1By similarity | 1 | |
Metal bindingi | 79 | Manganese 1By similarity | 1 | |
Metal bindingi | 105 | Manganese 1By similarity | 1 | |
Metal bindingi | 105 | Manganese 2By similarity | 1 | |
Metal bindingi | 137 | Manganese 2By similarity | 1 | |
Active sitei | 138 | Proton donorBy similarity | 1 | |
Metal bindingi | 186 | Manganese 2By similarity | 1 | |
Metal bindingi | 260 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine phosphatase activity Source: TAIR
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Ligand | Manganese, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase PP1 isozyme 1Curated (EC:3.1.3.161 Publication)Alternative name(s): Type one protein phosphatase 11 Publication |
Gene namesi | Name:TOPP11 Publication Synonyms:PP1Imported Ordered Locus Names:At2g29400Imported ORF Names:F16P2.22Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G29400 |
TAIRi | locus:2043122, AT2G29400 |
Subcellular locationi
Nucleus
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- protein phosphatase type 1 complex Source: TAIR
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
No visible phenotype under normal growth conditions, but mutant seedlings exhibit hypersensitivity to abscisic acid (ABA) or salt treatments.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058797 | 1 – 318 | Serine/threonine-protein phosphatase PP1 isozyme 1Add BLAST | 318 |
Proteomic databases
PaxDbi | P30366 |
PRIDEi | P30366 |
ProteomicsDBi | 250579 |
Expressioni
Tissue specificityi
Expressed in cotyledons, leaves and flowers.1 Publication
Gene expression databases
ExpressionAtlasi | P30366, baseline and differential |
Genevisiblei | P30366, AT |
Interactioni
Subunit structurei
Interacts with I-2, SRK2D/SNRK2.2, SRK2I/SNRK2.3, SRK2A/SNRK2.4, SRK2E/SNRK2.6, SRK2C/SNRK2.8, PYL4, PYL9 and PYL11.
1 PublicationProtein-protein interaction databases
BioGRIDi | 2839, 1 interactor |
IntActi | P30366, 1 interactor |
STRINGi | 3702.AT2G29400.1 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P30366 |
OMAi | RNIARPM |
OrthoDBi | 766640at2759 |
PhylomeDBi | P30366 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
SUPFAMi | SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P30366-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEKPAQEQE QKRAMEPAVL DDIIRRLVEF RNTRPGSGKQ VHLSEGEIRQ
60 70 80 90 100
LCAVSKEIFL QQPNLLELEA PIKICGDIHG QYSDLLRLFE YGGFPPEANY
110 120 130 140 150
LFLGDYVDRG KQSLETICLL LAYKIKYPEN FFLLRGNHES ASINRIYGFY
160 170 180 190 200
DECKRRFNVR LWKIFTDCFN CLPVAALIDD RILCMHGGIS PELKSLDQIR
210 220 230 240 250
NIARPMDIPE SGLVCDLLWS DPSGDVGWGM NDRGVSYTFG ADKVAEFLEK
260 270 280 290 300
HDMDLICRAH QVVEDGYEFF AERQLVTVFS APNYCGEFDN AGAMMSIDES
310
LMCSFQILKP SEKKSPFL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64328 mRNA Translation: CAA45611.1 M93408 mRNA Translation: AAA32723.1 AC004561 Genomic DNA Translation: AAC95198.1 CP002685 Genomic DNA Translation: AEC08250.1 AY080871 mRNA Translation: AAL87342.1 AY142593 mRNA Translation: AAN13162.1 |
PIRi | S20882 |
RefSeqi | NP_180501.1, NM_128494.5 |
Genome annotation databases
EnsemblPlantsi | AT2G29400.1; AT2G29400.1; AT2G29400 |
GeneIDi | 817489 |
Gramenei | AT2G29400.1; AT2G29400.1; AT2G29400 |
KEGGi | ath:AT2G29400 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64328 mRNA Translation: CAA45611.1 M93408 mRNA Translation: AAA32723.1 AC004561 Genomic DNA Translation: AAC95198.1 CP002685 Genomic DNA Translation: AEC08250.1 AY080871 mRNA Translation: AAL87342.1 AY142593 mRNA Translation: AAN13162.1 |
PIRi | S20882 |
RefSeqi | NP_180501.1, NM_128494.5 |
3D structure databases
SMRi | P30366 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2839, 1 interactor |
IntActi | P30366, 1 interactor |
STRINGi | 3702.AT2G29400.1 |
Proteomic databases
PaxDbi | P30366 |
PRIDEi | P30366 |
ProteomicsDBi | 250579 |
Genome annotation databases
EnsemblPlantsi | AT2G29400.1; AT2G29400.1; AT2G29400 |
GeneIDi | 817489 |
Gramenei | AT2G29400.1; AT2G29400.1; AT2G29400 |
KEGGi | ath:AT2G29400 |
Organism-specific databases
Araporti | AT2G29400 |
TAIRi | locus:2043122, AT2G29400 |
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P30366 |
OMAi | RNIARPM |
OrthoDBi | 766640at2759 |
PhylomeDBi | P30366 |
Miscellaneous databases
PROi | PR:P30366 |
Gene expression databases
ExpressionAtlasi | P30366, baseline and differential |
Genevisiblei | P30366, AT |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
SUPFAMi | SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PP11_ARATH | |
Accessioni | P30366Primary (citable) accession number: P30366 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | September 29, 2021 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families