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Protein

RNA polymerase sigma-54 factor 2

Gene

rpoN2

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi403 – 422H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processNitrogen fixation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciBDIA224911:G1G3J-730-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor 2
Gene namesi
Name:rpoN2
Ordered Locus Names:blr0723
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055251 – 537RNA polymerase sigma-54 factor 2Add BLAST537

Proteomic databases

PRIDEiP30333

Expressioni

Inductioni

Negatively autoregulated.

Interactioni

Protein-protein interaction databases

STRINGi224911.blr0723

Structurei

3D structure databases

ProteinModelPortaliP30333
SMRiP30333
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi492 – 500RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 29Gln-richAdd BLAST19

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW Bacteria
COG1508 LUCA
HOGENOMiHOG000071904
InParanoidiP30333
KOiK03092
OMAiVTTQKFM
OrthoDBiPOG091H02EW
PhylomeDBiP30333

Family and domain databases

Gene3Di1.10.10.1330, 1 hit
InterProiView protein in InterPro
IPR000394 RNA_pol_sigma_54
IPR007046 RNA_pol_sigma_54_core-bd
IPR007634 RNA_pol_sigma_54_DNA-bd
IPR038709 RpoN_core-bd_sf
PANTHERiPTHR32248 PTHR32248, 1 hit
PfamiView protein in Pfam
PF00309 Sigma54_AID, 1 hit
PF04963 Sigma54_CBD, 1 hit
PF04552 Sigma54_DBD, 1 hit
PIRSFiPIRSF000774 RpoN, 1 hit
PRINTSiPR00045 SIGMA54FCT
TIGRFAMsiTIGR02395 rpoN_sigma, 1 hit
PROSITEiView protein in PROSITE
PS00717 SIGMA54_1, 1 hit
PS00718 SIGMA54_2, 1 hit
PS50044 SIGMA54_3, 1 hit

Sequencei

Sequence statusi: Complete.

P30333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTQRLEFR QSQSLVMTPQ LMQAIKLLQL SNLDLTTFVE EELERNPLLE
60 70 80 90 100
RANDEASGGE APAEAGQFSD SDGGHNDEPG GGPGEAFEPG QEEWMSKDLG
110 120 130 140 150
TRAEIEQTLD TGLDNVFSEE PAEAAARNAQ DAAPTTYTEW GGGASGDEDY
160 170 180 190 200
NLEAFVAAEV TLGDHLAEQL SVAFTAPAQR MIGQYLIDLV DEAGYLPPDL
210 220 230 240 250
GQAAERLGAS QQEVEDVLAV LQKFDPPGVC ARNLSECLAI QLRELDRYDP
260 270 280 290 300
AMQALVEHLD LLAKRDIAGL RKVCGVDDED IADMIGEIRR LNPKPGMKFG
310 320 330 340 350
AARLQTMVPD VYVRPGPDGG WHVELNSDTL PRVLVNQTYY SELSKKIGKD
360 370 380 390 400
GDKSYFTDAL QNATWLVRAL DQRARTILKV ATEIVRQQDG FFTHGVAHLR
410 420 430 440 450
PLNLKAVADA IQMHESTVSR VTANKYMATN RGTFELKYFF TASIASADGG
460 470 480 490 500
EAHSAEAVRH HIKQLIDSEA PAAILSDDTI VERLRASGID IARRTVAKYR
510 520 530
EAMRIPSSVQ RRRDKQSALG NVLSTAMSDR SRNPEPA
Length:537
Mass (Da):58,832
Last modified:April 1, 1993 - v1
Checksum:i163D40BDAAE540EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59243 Genomic DNA Translation: AAA26241.1
BA000040 Genomic DNA Translation: BAC45988.1
PIRiB38179
RefSeqiNP_767363.1, NC_004463.1
WP_011083548.1, NZ_CP011360.1

Genome annotation databases

EnsemblBacteriaiBAC45988; BAC45988; BAC45988
GeneIDi1054195
KEGGibja:blr0723
PATRICifig|224911.44.peg.95

Similar proteinsi

Entry informationi

Entry nameiRP55_BRADU
AccessioniPrimary (citable) accession number: P30333
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 20, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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