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Protein

M-phase inducer phosphatase 2

Gene

Cdc25b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Stimulated by B-type cyclins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei483By similarity1

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • female meiosis I Source: MGI
  • mitotic cell cycle Source: MGI
  • oocyte maturation Source: MGI
  • positive regulation of cell cycle G2/M phase transition Source: InterPro
  • positive regulation of cytokinesis Source: MGI
  • positive regulation of mitotic cell cycle Source: MGI
  • positive regulation of protein kinase activity Source: MGI
  • protein phosphorylation Source: MGI

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-MMU-69656 Cyclin A:Cdk2-associated events at S phase entry

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 2 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25B
Gene namesi
Name:Cdc25b
Synonyms:Cdc25m2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:99701 Cdc25b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2723

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001986451 – 576M-phase inducer phosphatase 2Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42PhosphoserineBy similarity1
Modified residuei167Phosphoserine; by MELKBy similarity1
Modified residuei248PhosphoserineBy similarity1
Modified residuei321Phosphoserine; by MELK and MAPK14By similarity1
Modified residuei351Phosphoserine; by AURKABy similarity1
Modified residuei372Phosphoserine; by BRSK1 and MAPK14By similarity1
Modified residuei559PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-351 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-167 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP30306
PaxDbiP30306
PRIDEiP30306

PTM databases

iPTMnetiP30306
PhosphoSitePlusiP30306

Expressioni

Tissue specificityi

Expressed predominantly in spleen, lung, heart, brain, intestine, and muscle.

Developmental stagei

Detected at the one-cell stage followed by a decrease in signal intensity at the two-cell stage. Detectable at higher level in the four-cell stage and expressed through the eight-cell, 16-cell and morula stages. Maximal expression at the blastocyst stage.

Gene expression databases

BgeeiENSMUSG00000027330
CleanExiMM_CDC25B
ExpressionAtlasiP30306 baseline and differential
GenevisibleiP30306 MM

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198622, 3 interactors
IntActiP30306, 3 interactors
MINTiP30306
STRINGi10090.ENSMUSP00000028804

Chemistry databases

BindingDBiP30306

Structurei

3D structure databases

ProteinModelPortaliP30306
SMRiP30306
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini427 – 534RhodanesePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated

Phylogenomic databases

eggNOGiKOG3772 Eukaryota
COG5105 LUCA
GeneTreeiENSGT00390000018747
HOGENOMiHOG000082672
HOVERGENiHBG052501
InParanoidiP30306
KOiK05866
OMAiTHALAEW
OrthoDBiEOG091G0H0D
PhylomeDBiP30306
TreeFamiTF101056

Family and domain databases

CDDicd01530 Cdc25, 1 hit
Gene3Di3.40.250.10, 1 hit
InterProiView protein in InterPro
IPR000751 MPI_Phosphatase
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
PfamiView protein in Pfam
PF06617 M-inducer_phosp, 1 hit
PF00581 Rhodanese, 1 hit
PRINTSiPR00716 MPIPHPHTASE
SMARTiView protein in SMART
SM00450 RHOD, 1 hit
SUPFAMiSSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P30306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS
60 70 80 90 100
PVTTLTQTMH NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE
110 120 130 140 150
SSESSDAGLC MDSPSPVDPQ MAERTFEQAI QAASRVIQNE QFTIKRFRSL
160 170 180 190 200
PVRLLEHSPV LQSITNSRAL DSWRKTEAGY RAAANSPGED KENDGYIFKM
210 220 230 240 250
PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW KMEVEELSPV
260 270 280 290 300
AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD
310 320 330 340 350
KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK
360 370 380 390 400
SVTPLEEQQL EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT
410 420 430 440 450
VDGKHQDLKY ISPETMVALL TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA
460 470 480 490 500
VNLPLERDAE TFLLQRPIMP CSLDKRIILI FHCEFSSERG PRMCRFIRER
510 520 530 540 550
DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP MNHEAFRDEL
560 570
RNFRLKTRSW AGERSRRELC SRLQDQ
Length:576
Mass (Da):65,490
Last modified:April 1, 1993 - v1
Checksum:i5086B676581CD570
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti532F → L in AAH02287 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S93521 mRNA Translation: AAB22026.1
BC002287 mRNA Translation: AAH02287.1
CCDSiCCDS16758.1
PIRiA42236
RefSeqiNP_075606.1, NM_023117.4
UniGeneiMm.38444

Genome annotation databases

EnsembliENSMUST00000028804; ENSMUSP00000028804; ENSMUSG00000027330
GeneIDi12531
KEGGimmu:12531
UCSCiuc008mky.3 mouse

Similar proteinsi

Entry informationi

Entry nameiMPIP2_MOUSE
AccessioniPrimary (citable) accession number: P30306
Secondary accession number(s): Q99LP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 20, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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