UniProtKB - P30305 (MPIP2_HUMAN)
Protein
M-phase inducer phosphatase 2
Gene
CDC25B
Organism
Homo sapiens (Human)
Status
Functioni
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.1 Publication
Catalytic activityi
- EC:3.1.3.48
Activity regulationi
Stimulated by B-type cyclins.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 487 | 1 |
GO - Molecular functioni
- phosphoprotein phosphatase activity Source: Reactome
- protein kinase binding Source: UniProtKB
- protein tyrosine phosphatase activity Source: GO_Central
GO - Biological processi
- cell division Source: UniProtKB-KW
- female meiosis I Source: Ensembl
- G2/M transition of mitotic cell cycle Source: GO_Central
- mitotic cell cycle Source: UniProtKB
- oocyte maturation Source: Ensembl
- positive regulation of cell population proliferation Source: ProtInc
- positive regulation of cytokinesis Source: UniProtKB
- positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
- positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
- positive regulation of mitotic cell cycle Source: UniProtKB
- positive regulation of protein kinase activity Source: Ensembl
- protein phosphorylation Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
BRENDAi | 3.1.3.48, 2681 |
PathwayCommonsi | P30305 |
Reactomei | R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69656, Cyclin A:Cdk2-associated events at S phase entry R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models |
SIGNORi | P30305 |
Names & Taxonomyi
Protein namesi | Recommended name: M-phase inducer phosphatase 2 (EC:3.1.3.48)Alternative name(s): Dual specificity phosphatase Cdc25B |
Gene namesi | Name:CDC25B Synonyms:CDC25HU2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000101224.17 |
HGNCi | HGNC:1726, CDC25B |
MIMi | 116949, gene |
neXtProti | NX_P30305 |
Subcellular locationi
Cytoskeleton
- centrosome 3 Publications
- spindle pole 1 Publication
Cytoskeleton
- centrosome Source: UniProtKB
- spindle pole Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Organism-specific databases
DisGeNETi | 994 |
OpenTargetsi | ENSG00000101224 |
PharmGKBi | PA26260 |
Miscellaneous databases
Pharosi | P30305, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4804 |
DrugBanki | DB03661, L-cysteic acid |
DrugCentrali | P30305 |
Polymorphism and mutation databases
BioMutai | CDC25B |
DMDMi | 21264471 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198644 | 1 – 580 | M-phase inducer phosphatase 2Add BLAST | 580 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 42 | PhosphoserineBy similarity | 1 | |
Modified residuei | 169 | Phosphoserine; by MELK1 Publication | 1 | |
Modified residuei | 249 | PhosphoserineCombined sources | 1 | |
Modified residuei | 323 | Phosphoserine; by MELK and MAPK14Combined sources3 Publications | 1 | |
Modified residuei | 353 | Phosphoserine; by AURKACombined sources1 Publication | 1 | |
Modified residuei | 375 | Phosphoserine; by BRSK1 and MAPK14Combined sources2 Publications | 1 | |
Modified residuei | 470 | PhosphoserineCombined sources | 1 | |
Modified residuei | 563 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.8 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
CPTACi | CPTAC-1253 CPTAC-1254 |
EPDi | P30305 |
jPOSTi | P30305 |
MassIVEi | P30305 |
MaxQBi | P30305 |
PaxDbi | P30305 |
PeptideAtlasi | P30305 |
PRIDEi | P30305 |
ProteomicsDBi | 54654 [P30305-1] 54655 [P30305-2] 54656 [P30305-3] 54657 [P30305-4] |
PTM databases
DEPODi | CDC25B |
iPTMneti | P30305 |
PhosphoSitePlusi | P30305 |
Expressioni
Gene expression databases
Bgeei | ENSG00000101224, Expressed in right hemisphere of cerebellum and 231 other tissues |
ExpressionAtlasi | P30305, baseline and differential |
Genevisiblei | P30305, HS |
Organism-specific databases
HPAi | ENSG00000101224, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with MAPK14 and 14-3-3 proteins.
1 PublicationBinary interactionsi
Hide detailsP30305
GO - Molecular functioni
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107429, 122 interactors |
DIPi | DIP-323N |
ELMi | P30305 |
IntActi | P30305, 18 interactors |
MINTi | P30305 |
STRINGi | 9606.ENSP00000245960 |
Chemistry databases
BindingDBi | P30305 |
Miscellaneous databases
RNActi | P30305, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P30305 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30305 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 431 – 538 | RhodanesePROSITE-ProRule annotationAdd BLAST | 108 |
Sequence similaritiesi
Belongs to the MPI phosphatase family.Curated
Phylogenomic databases
eggNOGi | KOG3772, Eukaryota |
GeneTreei | ENSGT00940000158524 |
InParanoidi | P30305 |
OMAi | RSKSFCH |
PhylomeDBi | P30305 |
TreeFami | TF101056 |
Family and domain databases
CDDi | cd01530, Cdc25, 1 hit |
Gene3Di | 3.40.250.10, 1 hit |
InterProi | View protein in InterPro IPR000751, MPI_Phosphatase IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf |
Pfami | View protein in Pfam PF06617, M-inducer_phosp, 1 hit PF00581, Rhodanese, 1 hit |
PRINTSi | PR00716, MPIPHPHTASE |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 3 (identifier: P30305-1) [UniParc]FASTAAdd to basket
Also known as: CDC25B3
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEVPQPEPAP GSALSPAGVC GGAQRPGHLP GLLLGSHGLL GSPVRAAASS
60 70 80 90 100
PVTTLTQTMH DLAGLGSETP KSQVGTLLFR SRSRLTHLSL SRRASESSLS
110 120 130 140 150
SESSESSDAG LCMDSPSPMD PHMAEQTFEQ AIQAASRIIR NEQFAIRRFQ
160 170 180 190 200
SMPVRLLGHS PVLRNITNSQ APDGRRKSEA GSGAASSSGE DKENDGFVFK
210 220 230 240 250
MPWKPTHPSS THALAEWASR REAFAQRPSS APDLMCLSPD RKMEVEELSP
260 270 280 290 300
LALGRFSLTP AEGDTEEDDG FVDILESDLK DDDAVPPGME SLISAPLVKT
310 320 330 340 350
LEKEEEKDLV MYSKCQRLFR SPSMPCSVIR PILKRLERPQ DRDTPVQNKR
360 370 380 390 400
RRSVTPPEEQ QEAEEPKARV LRSKSLCHDE IENLLDSDHR ELIGDYSKAF
410 420 430 440 450
LLQTVDGKHQ DLKYISPETM VALLTGKFSN IVDKFVIVDC RYPYEYEGGH
460 470 480 490 500
IKTAVNLPLE RDAESFLLKS PIAPCSLDKR VILIFHCEFS SERGPRMCRF
510 520 530 540 550
IRERDRAVND YPSLYYPEMY ILKGGYKEFF PQHPNFCEPQ DYRPMNHEAF
560 570 580
KDELKTFRLK TRSWAGERSR RELCSRLQDQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG8JLH2 | G8JLH2_HUMAN | Protein-tyrosine-phosphatase | CDC25B | 489 | Annotation score: | ||
B4DIG0 | B4DIG0_HUMAN | Protein-tyrosine-phosphatase | CDC25B | 516 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 575 | S → D in AAB21139 (PubMed:1662986).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020933 | 548 | E → K1 PublicationCorresponds to variant dbSNP:rs11570019Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000861 | 68 – 81 | Missing in isoform 1 and isoform 4. 2 PublicationsAdd BLAST | 14 | |
Alternative sequenceiVSP_000862 | 154 – 194 | Missing in isoform 2. 1 PublicationAdd BLAST | 41 | |
Alternative sequenceiVSP_012587 | 194 | N → NVRFWKAGVGALREEEGACW GGSLACEDPPLPSWLQ in isoform 4. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81934 mRNA Translation: AAA58416.1 S78187 mRNA Translation: AAB21139.1 X96436 Genomic DNA Translation: CAA65303.1 Z68092 mRNA Translation: CAA92108.1 AY494082 Genomic DNA Translation: AAR26469.1 AL109804 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10491.1 CH471133 Genomic DNA Translation: EAX10492.1 CH471133 Genomic DNA Translation: EAX10493.1 CH471133 Genomic DNA Translation: EAX10494.1 CH471133 Genomic DNA Translation: EAX10496.1 CH471133 Genomic DNA Translation: EAX10497.1 CH471133 Genomic DNA Translation: EAX10498.1 CH471133 Genomic DNA Translation: EAX10499.1 BC006395 mRNA Translation: AAH06395.1 BC009953 mRNA Translation: AAH09953.1 BC051711 mRNA Translation: AAH51711.1 AF036233 Genomic DNA Translation: AAB94622.1 AF036233 Genomic DNA Translation: AAB94624.1 |
CCDSi | CCDS13065.1 [P30305-2] CCDS13066.1 [P30305-3] CCDS13067.1 [P30305-1] |
PIRi | B41648 |
RefSeqi | NP_001274448.1, NM_001287519.1 NP_001274453.1, NM_001287524.1 NP_004349.1, NM_004358.4 [P30305-2] NP_068658.1, NM_021872.3 [P30305-3] NP_068659.1, NM_021873.3 [P30305-1] |
Genome annotation databases
Ensembli | ENST00000245960; ENSP00000245960; ENSG00000101224 [P30305-1] ENST00000340833; ENSP00000339170; ENSG00000101224 [P30305-3] ENST00000439880; ENSP00000405972; ENSG00000101224 [P30305-2] |
GeneIDi | 994 |
KEGGi | hsa:994 |
UCSCi | uc002wjn.5, human [P30305-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81934 mRNA Translation: AAA58416.1 S78187 mRNA Translation: AAB21139.1 X96436 Genomic DNA Translation: CAA65303.1 Z68092 mRNA Translation: CAA92108.1 AY494082 Genomic DNA Translation: AAR26469.1 AL109804 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10491.1 CH471133 Genomic DNA Translation: EAX10492.1 CH471133 Genomic DNA Translation: EAX10493.1 CH471133 Genomic DNA Translation: EAX10494.1 CH471133 Genomic DNA Translation: EAX10496.1 CH471133 Genomic DNA Translation: EAX10497.1 CH471133 Genomic DNA Translation: EAX10498.1 CH471133 Genomic DNA Translation: EAX10499.1 BC006395 mRNA Translation: AAH06395.1 BC009953 mRNA Translation: AAH09953.1 BC051711 mRNA Translation: AAH51711.1 AF036233 Genomic DNA Translation: AAB94622.1 AF036233 Genomic DNA Translation: AAB94624.1 |
CCDSi | CCDS13065.1 [P30305-2] CCDS13066.1 [P30305-3] CCDS13067.1 [P30305-1] |
PIRi | B41648 |
RefSeqi | NP_001274448.1, NM_001287519.1 NP_001274453.1, NM_001287524.1 NP_004349.1, NM_004358.4 [P30305-2] NP_068658.1, NM_021872.3 [P30305-3] NP_068659.1, NM_021873.3 [P30305-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CWR | X-ray | 2.10 | A | 370-580 | [»] | |
1CWS | X-ray | 2.00 | A | 370-580 | [»] | |
1CWT | X-ray | 2.30 | A | 388-565 | [»] | |
1QB0 | X-ray | 1.91 | A | 370-580 | [»] | |
1YM9 | X-ray | 2.00 | A | 391-564 | [»] | |
1YMD | X-ray | 1.70 | A | 391-564 | [»] | |
1YMK | X-ray | 1.70 | A | 391-564 | [»] | |
1YML | X-ray | 1.70 | A | 391-564 | [»] | |
1YS0 | X-ray | 2.00 | A | 391-564 | [»] | |
2A2K | X-ray | 1.52 | A | 391-564 | [»] | |
2IFD | X-ray | 2.00 | A | 391-564 | [»] | |
2IFV | X-ray | 1.60 | A | 391-564 | [»] | |
2UZQ | X-ray | 2.38 | A/B/C/D/E/F | 391-580 | [»] | |
3FQT | X-ray | 1.80 | C | 38-46 | [»] | |
3FQU | X-ray | 1.80 | C | 38-46 | [»] | |
4WH7 | X-ray | 1.62 | A | 386-565 | [»] | |
4WH9 | X-ray | 1.50 | A | 386-565 | [»] | |
SMRi | P30305 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107429, 122 interactors |
DIPi | DIP-323N |
ELMi | P30305 |
IntActi | P30305, 18 interactors |
MINTi | P30305 |
STRINGi | 9606.ENSP00000245960 |
Chemistry databases
BindingDBi | P30305 |
ChEMBLi | CHEMBL4804 |
DrugBanki | DB03661, L-cysteic acid |
DrugCentrali | P30305 |
PTM databases
DEPODi | CDC25B |
iPTMneti | P30305 |
PhosphoSitePlusi | P30305 |
Polymorphism and mutation databases
BioMutai | CDC25B |
DMDMi | 21264471 |
Proteomic databases
CPTACi | CPTAC-1253 CPTAC-1254 |
EPDi | P30305 |
jPOSTi | P30305 |
MassIVEi | P30305 |
MaxQBi | P30305 |
PaxDbi | P30305 |
PeptideAtlasi | P30305 |
PRIDEi | P30305 |
ProteomicsDBi | 54654 [P30305-1] 54655 [P30305-2] 54656 [P30305-3] 54657 [P30305-4] |
Protocols and materials databases
Antibodypediai | 3625, 920 antibodies |
CPTCi | P30305, 2 antibodies |
DNASUi | 994 |
Genome annotation databases
Ensembli | ENST00000245960; ENSP00000245960; ENSG00000101224 [P30305-1] ENST00000340833; ENSP00000339170; ENSG00000101224 [P30305-3] ENST00000439880; ENSP00000405972; ENSG00000101224 [P30305-2] |
GeneIDi | 994 |
KEGGi | hsa:994 |
UCSCi | uc002wjn.5, human [P30305-1] |
Organism-specific databases
CTDi | 994 |
DisGeNETi | 994 |
EuPathDBi | HostDB:ENSG00000101224.17 |
GeneCardsi | CDC25B |
HGNCi | HGNC:1726, CDC25B |
HPAi | ENSG00000101224, Low tissue specificity |
MIMi | 116949, gene |
neXtProti | NX_P30305 |
OpenTargetsi | ENSG00000101224 |
PharmGKBi | PA26260 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3772, Eukaryota |
GeneTreei | ENSGT00940000158524 |
InParanoidi | P30305 |
OMAi | RSKSFCH |
PhylomeDBi | P30305 |
TreeFami | TF101056 |
Enzyme and pathway databases
BRENDAi | 3.1.3.48, 2681 |
PathwayCommonsi | P30305 |
Reactomei | R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69656, Cyclin A:Cdk2-associated events at S phase entry R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models |
SIGNORi | P30305 |
Miscellaneous databases
BioGRID-ORCSi | 994, 136 hits in 849 CRISPR screens |
ChiTaRSi | CDC25B, human |
EvolutionaryTracei | P30305 |
GeneWikii | CDC25B |
GenomeRNAii | 994 |
Pharosi | P30305, Tchem |
PROi | PR:P30305 |
RNActi | P30305, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000101224, Expressed in right hemisphere of cerebellum and 231 other tissues |
ExpressionAtlasi | P30305, baseline and differential |
Genevisiblei | P30305, HS |
Family and domain databases
CDDi | cd01530, Cdc25, 1 hit |
Gene3Di | 3.40.250.10, 1 hit |
InterProi | View protein in InterPro IPR000751, MPI_Phosphatase IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf |
Pfami | View protein in Pfam PF06617, M-inducer_phosp, 1 hit PF00581, Rhodanese, 1 hit |
PRINTSi | PR00716, MPIPHPHTASE |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPIP2_HUMAN | |
Accessioni | P30305Primary (citable) accession number: P30305 Secondary accession number(s): D3DVY1 Q9BRA6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | May 27, 2002 | |
Last modified: | December 2, 2020 | |
This is version 209 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations