UniProtKB - P30304 (MPIP1_HUMAN)
M-phase inducer phosphatase 1
CDC25A
Functioni
Catalytic activityi
- EC:3.1.3.48
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 431 | 1 |
GO - Molecular functioni
- chaperone binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- protein tyrosine phosphatase activity Source: GO_Central
GO - Biological processi
- cell division Source: UniProtKB-KW
- cell population proliferation Source: UniProtKB
- cellular response to UV Source: UniProtKB
- G1/S transition of mitotic cell cycle Source: Reactome
- G2/M transition of mitotic cell cycle Source: GO_Central
- positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
- positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
- protein deubiquitination Source: Reactome
- regulation of cell cycle Source: Reactome
- regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
- response to radiation Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
BRENDAi | 3.1.3.48, 2681 |
PathwayCommonsi | P30304 |
Reactomei | R-HSA-1362277, Transcription of E2F targets under negative control by DREAM complex R-HSA-156711, Polo-like kinase mediated events R-HSA-176187, Activation of ATR in response to replication stress R-HSA-5689880, Ub-specific processing proteases R-HSA-69202, Cyclin E associated events during G1/S transition R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-69656, Cyclin A:Cdk2-associated events at S phase entry R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models |
SIGNORi | P30304 |
Names & Taxonomyi
Protein namesi | Recommended name: M-phase inducer phosphatase 1 (EC:3.1.3.48)Alternative name(s): Dual specificity phosphatase Cdc25A |
Gene namesi | Name:CDC25A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1725, CDC25A |
MIMi | 116947, gene |
neXtProti | NX_P30304 |
VEuPathDBi | HostDB:ENSG00000164045.11 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 76 | S → A: Abolishes ubiquitination and impairs CHEK1-dependent degradation following checkpoint activation. 2 Publications | 1 | |
Mutagenesisi | 79 | S → A: Abrogates interactions with BTRC and FBXW11 and prevents ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 81 | D → A: Abrogates interactions with BTRC and FBXW11 and prevents ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 82 | S → A: Abrogates interactions with BTRC and FBXW11 and prevents ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 124 | S → A: Abrogates phosphorylation by CHEK2 and infrared-induced degradation. Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-178; A-279 and A-293. 4 Publications | 1 | |
Mutagenesisi | 141 – 143 | KEN → AAA: Prevents ubiquitination and subsequent degradation by the APC/C ubiquitin ligase complex. 1 Publication | 3 | |
Mutagenesisi | 178 | S → A: Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-124; A-279 and A-293. Abrogates 14-3-3 protein binding. 3 Publications | 1 | |
Mutagenesisi | 279 | S → A: Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-124; A-178 and A-293. 1 Publication | 1 | |
Mutagenesisi | 293 | S → A: Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-124; A-178 and A-279. 1 Publication | 1 | |
Mutagenesisi | 431 | C → S: Abolishes phosphatase activity. 2 Publications | 1 | |
Mutagenesisi | 507 | T → A: Abrogates 14-3-3 protein binding; increases binding to cyclin B1. 1 Publication | 1 | |
Mutagenesisi | 513 | S → A: Increased stability following IR treatment. 1 Publication | 1 | |
Mutagenesisi | 513 | S → D: Mimicks phosphorylation state, leading to promote degradation following IR treatment. 1 Publication | 1 | |
Mutagenesisi | 514 | K → L: Abrogates binding to CCNB1; when associated with L-520. 1 Publication | 1 | |
Mutagenesisi | 519 | S → A: Increased stability following IR treatment. 1 Publication | 1 | |
Mutagenesisi | 519 | S → D: Mimicks phosphorylation state, leading to promote degradation following IR treatment. 1 Publication | 1 | |
Mutagenesisi | 520 | R → L: Abrogates binding to CCNB1; when associated with L-514. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 993 |
OpenTargetsi | ENSG00000164045 |
PharmGKBi | PA26259 |
Miscellaneous databases
Pharosi | P30304, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3775 |
DrugCentrali | P30304 |
Genetic variation databases
BioMutai | CDC25A |
DMDMi | 50403734 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198641 | 1 – 524 | M-phase inducer phosphatase 1Add BLAST | 524 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 76 | Phosphoserine; by CHEK12 Publications | 1 | |
Modified residuei | 79 | Phosphoserine; by NEK111 Publication | 1 | |
Modified residuei | 82 | Phosphoserine; by NEK112 Publications | 1 | |
Modified residuei | 88 | Phosphoserine; by NEK112 Publications | 1 | |
Modified residuei | 107 | PhosphoserineCombined sources | 1 | |
Modified residuei | 124 | Phosphoserine; by CHEK1 and CHEK25 Publications | 1 | |
Modified residuei | 178 | Phosphoserine; by CHEK13 Publications | 1 | |
Modified residuei | 279 | Phosphoserine; by CHEK1 and CHEK21 Publication | 1 | |
Modified residuei | 293 | Phosphoserine; by CHEK1 and CHEK21 Publication | 1 | |
Modified residuei | 321 | PhosphoserineCombined sources | 1 | |
Modified residuei | 507 | Phosphothreonine; by CHEK11 Publication | 1 | |
Modified residuei | 513 | Phosphoserine; by PLK31 Publication | 1 | |
Modified residuei | 519 | Phosphoserine; by PLK31 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P30304 |
jPOSTi | P30304 |
MassIVEi | P30304 |
MaxQBi | P30304 |
PaxDbi | P30304 |
PeptideAtlasi | P30304 |
PRIDEi | P30304 |
ProteomicsDBi | 54652 [P30304-1] 54653 [P30304-2] |
PTM databases
DEPODi | CDC25A |
iPTMneti | P30304 |
PhosphoSitePlusi | P30304 |
Expressioni
Gene expression databases
Bgeei | ENSG00000164045, Expressed in secondary oocyte and 149 other tissues |
ExpressionAtlasi | P30304, baseline and differential |
Genevisiblei | P30304, HS |
Organism-specific databases
HPAi | ENSG00000164045, Tissue enhanced (bone marrow, lymphoid tissue, testis) |
Interactioni
Subunit structurei
Interacts with CCNB1/cyclin B1.
Interacts with YWHAE/14-3-3 epsilon when phosphorylated.
Interacts with CUL1 specifically when CUL1 is neddylated and active.
Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage.
Interacts with PIM1.
Interacts with CHEK2; mediates CDC25A phosphorylation and degradation in response to infrared-induced DNA damages.
Interacts with HSP90AB1; prevents heat shock-mediated CDC25A degradation and contributes to cell cycle progression (PubMed:22843495).
5 PublicationsBinary interactionsi
Hide detailsP30304
With | #Exp. | IntAct |
---|---|---|
NAA11 [Q9BSU3] | 3 | EBI-747671,EBI-2585120 |
RAF1 [P04049] | 4 | EBI-747671,EBI-365996 |
YWHAB [P31946] | 10 | EBI-747671,EBI-359815 |
YWHAQ [P27348] | 3 | EBI-747671,EBI-359854 |
YWHAZ [P63104] | 2 | EBI-747671,EBI-347088 |
GO - Molecular functioni
- chaperone binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107428, 128 interactors |
DIPi | DIP-166N |
ELMi | P30304 |
IntActi | P30304, 46 interactors |
MINTi | P30304 |
STRINGi | 9606.ENSP00000303706 |
Chemistry databases
BindingDBi | P30304 |
Miscellaneous databases
RNActi | P30304, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P30304 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30304 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 376 – 482 | RhodanesePROSITE-ProRule annotationAdd BLAST | 107 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 74 – 84 | PhosphodegronAdd BLAST | 11 | |
Motifi | 141 – 143 | KEN box | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3772, Eukaryota |
GeneTreei | ENSGT00940000160737 |
HOGENOMi | CLU_014464_0_1_1 |
InParanoidi | P30304 |
OMAi | GRTTQKR |
PhylomeDBi | P30304 |
TreeFami | TF101056 |
Family and domain databases
CDDi | cd01530, Cdc25, 1 hit |
Gene3Di | 3.40.250.10, 1 hit |
InterProi | View protein in InterPro IPR000751, MPI_Phosphatase IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf |
Pfami | View protein in Pfam PF06617, M-inducer_phosp, 1 hit PF00581, Rhodanese, 1 hit |
PRINTSi | PR00716, MPIPHPHTASE |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELGPEPPHR RRLLFACSPP PASQPVVKAL FGASAAGGLS PVTNLTVTMD
60 70 80 90 100
QLQGLGSDYE QPLEVKNNSN LQRMGSSEST DSGFCLDSPG PLDSKENLEN
110 120 130 140 150
PMRRIHSLPQ KLLGCSPALK RSHSDSLDHD IFQLIDPDEN KENEAFEFKK
160 170 180 190 200
PVRPVSRGCL HSHGLQEGKD LFTQRQNSAP ARMLSSNERD SSEPGNFIPL
210 220 230 240 250
FTPQSPVTAT LSDEDDGFVD LLDGENLKNE EETPSCMASL WTAPLVMRTT
260 270 280 290 300
NLDNRCKLFD SPSLCSSSTR SVLKRPERSQ EESPPGSTKR RKSMSGASPK
310 320 330 340 350
ESTNPEKAHE TLHQSLSLAS SPKGTIENIL DNDPRDLIGD FSKGYLFHTV
360 370 380 390 400
AGKHQDLKYI SPEIMASVLN GKFANLIKEF VIIDCRYPYE YEGGHIKGAV
410 420 430 440 450
NLHMEEEVED FLLKKPIVPT DGKRVIVVFH CEFSSERGPR MCRYVRERDR
460 470 480 490 500
LGNEYPKLHY PELYVLKGGY KEFFMKCQSY CEPPSYRPMH HEDFKEDLKK
510 520
FRTKSRTWAG EKSKREMYSR LKKL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JH94 | C9JH94_HUMAN | M-phase inducer phosphatase 1 | CDC25A | 143 | Annotation score: | ||
C9JFR5 | C9JFR5_HUMAN | M-phase inducer phosphatase 1 | CDC25A | 252 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 – 10 | EPPHR → SPAP in AAA58415 (PubMed:1836978).Curated | 5 | |
Sequence conflicti | 180 – 181 | PA → QL in AAA58415 (PubMed:1836978).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020932 | 88 | S → F1 PublicationCorresponds to variant dbSNP:rs3731499Ensembl. | 1 | |
Natural variantiVAR_023532 | 182 | R → G1 PublicationCorresponds to variant dbSNP:rs6771386Ensembl. | 1 | |
Natural variantiVAR_023533 | 182 | R → W. Corresponds to variant dbSNP:rs6771386Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000860 | 144 – 183 | Missing in isoform 2. 2 PublicationsAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81933 mRNA Translation: AAA58415.1 AY137580 mRNA Translation: AAN11305.1 AF527417 Genomic DNA Translation: AAM77917.1 BC007401 mRNA Translation: AAH07401.1 BC018642 mRNA Translation: AAH18642.1 AF277722 mRNA Translation: AAG41884.1 |
CCDSi | CCDS2760.1 [P30304-1] CCDS2761.1 [P30304-2] |
PIRi | A41648 |
RefSeqi | NP_001780.2, NM_001789.2 [P30304-1] NP_963861.1, NM_201567.1 [P30304-2] XP_011532618.1, XM_011534316.1 |
Genome annotation databases
Ensembli | ENST00000302506; ENSP00000303706; ENSG00000164045 [P30304-1] ENST00000351231; ENSP00000343166; ENSG00000164045 [P30304-2] |
GeneIDi | 993 |
KEGGi | hsa:993 |
UCSCi | uc003csh.2, human [P30304-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81933 mRNA Translation: AAA58415.1 AY137580 mRNA Translation: AAN11305.1 AF527417 Genomic DNA Translation: AAM77917.1 BC007401 mRNA Translation: AAH07401.1 BC018642 mRNA Translation: AAH18642.1 AF277722 mRNA Translation: AAG41884.1 |
CCDSi | CCDS2760.1 [P30304-1] CCDS2761.1 [P30304-2] |
PIRi | A41648 |
RefSeqi | NP_001780.2, NM_001789.2 [P30304-1] NP_963861.1, NM_201567.1 [P30304-2] XP_011532618.1, XM_011534316.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C25 | X-ray | 2.30 | A | 337-496 | [»] | |
SMRi | P30304 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107428, 128 interactors |
DIPi | DIP-166N |
ELMi | P30304 |
IntActi | P30304, 46 interactors |
MINTi | P30304 |
STRINGi | 9606.ENSP00000303706 |
Chemistry databases
BindingDBi | P30304 |
ChEMBLi | CHEMBL3775 |
DrugCentrali | P30304 |
PTM databases
DEPODi | CDC25A |
iPTMneti | P30304 |
PhosphoSitePlusi | P30304 |
Genetic variation databases
BioMutai | CDC25A |
DMDMi | 50403734 |
Proteomic databases
EPDi | P30304 |
jPOSTi | P30304 |
MassIVEi | P30304 |
MaxQBi | P30304 |
PaxDbi | P30304 |
PeptideAtlasi | P30304 |
PRIDEi | P30304 |
ProteomicsDBi | 54652 [P30304-1] 54653 [P30304-2] |
Protocols and materials databases
Antibodypediai | 1619, 1707 antibodies |
DNASUi | 993 |
Genome annotation databases
Ensembli | ENST00000302506; ENSP00000303706; ENSG00000164045 [P30304-1] ENST00000351231; ENSP00000343166; ENSG00000164045 [P30304-2] |
GeneIDi | 993 |
KEGGi | hsa:993 |
UCSCi | uc003csh.2, human [P30304-1] |
Organism-specific databases
CTDi | 993 |
DisGeNETi | 993 |
GeneCardsi | CDC25A |
HGNCi | HGNC:1725, CDC25A |
HPAi | ENSG00000164045, Tissue enhanced (bone marrow, lymphoid tissue, testis) |
MIMi | 116947, gene |
neXtProti | NX_P30304 |
OpenTargetsi | ENSG00000164045 |
PharmGKBi | PA26259 |
VEuPathDBi | HostDB:ENSG00000164045.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3772, Eukaryota |
GeneTreei | ENSGT00940000160737 |
HOGENOMi | CLU_014464_0_1_1 |
InParanoidi | P30304 |
OMAi | GRTTQKR |
PhylomeDBi | P30304 |
TreeFami | TF101056 |
Enzyme and pathway databases
BRENDAi | 3.1.3.48, 2681 |
PathwayCommonsi | P30304 |
Reactomei | R-HSA-1362277, Transcription of E2F targets under negative control by DREAM complex R-HSA-156711, Polo-like kinase mediated events R-HSA-176187, Activation of ATR in response to replication stress R-HSA-5689880, Ub-specific processing proteases R-HSA-69202, Cyclin E associated events during G1/S transition R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-69656, Cyclin A:Cdk2-associated events at S phase entry R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models |
SIGNORi | P30304 |
Miscellaneous databases
BioGRID-ORCSi | 993, 54 hits in 1003 CRISPR screens |
ChiTaRSi | CDC25A, human |
EvolutionaryTracei | P30304 |
GeneWikii | CDC25A |
GenomeRNAii | 993 |
Pharosi | P30304, Tchem |
PROi | PR:P30304 |
RNActi | P30304, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164045, Expressed in secondary oocyte and 149 other tissues |
ExpressionAtlasi | P30304, baseline and differential |
Genevisiblei | P30304, HS |
Family and domain databases
CDDi | cd01530, Cdc25, 1 hit |
Gene3Di | 3.40.250.10, 1 hit |
InterProi | View protein in InterPro IPR000751, MPI_Phosphatase IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf |
Pfami | View protein in Pfam PF06617, M-inducer_phosp, 1 hit PF00581, Rhodanese, 1 hit |
PRINTSi | PR00716, MPIPHPHTASE |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPIP1_HUMAN | |
Accessioni | P30304Primary (citable) accession number: P30304 Secondary accession number(s): Q8IZH5, Q96IL3, Q9H2F2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | July 19, 2004 | |
Last modified: | April 7, 2021 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families