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UniProtKB - P30304 (MPIP1_HUMAN)

Entry version 196 (12 Aug 2020)
Sequence version 2 (19 Jul 2004)
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Protein

M-phase inducer phosphatase 1

Gene

CDC25A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Stimulated by B-type cyclins. Stimulated by PIM1-mediated phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei4311

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		3.1.3.48, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P30304

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-1362277, Transcription of E2F targets under negative control by DREAM complex
R-HSA-156711, Polo-like kinase mediated events
R-HSA-176187, Activation of ATR in response to replication stress
R-HSA-5689880, Ub-specific processing proteases
R-HSA-69202, Cyclin E associated events during G1/S transition
R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition
R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-69656, Cyclin A:Cdk2-associated events at S phase entry
R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models

SIGNOR Signaling Network Open Resource

More...SIGNORi		P30304

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
M-phase inducer phosphatase 1 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25A
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CDC25A
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...EuPathDBi		HostDB:ENSG00000164045.11

Human Gene Nomenclature Database

More...HGNCi		HGNC:1725, CDC25A

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		116947, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P30304

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi76S → A: Abolishes ubiquitination and impairs CHEK1-dependent degradation following checkpoint activation. 2 Publications1
Mutagenesisi79S → A: Abrogates interactions with BTRC and FBXW11 and prevents ubiquitination. 1 Publication1
Mutagenesisi81D → A: Abrogates interactions with BTRC and FBXW11 and prevents ubiquitination. 1 Publication1
Mutagenesisi82S → A: Abrogates interactions with BTRC and FBXW11 and prevents ubiquitination. 1 Publication1
Mutagenesisi124S → A: Abrogates phosphorylation by CHEK2 and infrared-induced degradation. Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-178; A-279 and A-293. 4 Publications1
Mutagenesisi141 – 143KEN → AAA: Prevents ubiquitination and subsequent degradation by the APC/C ubiquitin ligase complex. 1 Publication3
Mutagenesisi178S → A: Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-124; A-279 and A-293. Abrogates 14-3-3 protein binding. 3 Publications1
Mutagenesisi279S → A: Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-124; A-178 and A-293. 1 Publication1
Mutagenesisi293S → A: Increases basal stability and impairs CHEK1-dependent degradation following checkpoint activation; when associated with A-124; A-178 and A-279. 1 Publication1
Mutagenesisi431C → S: Abolishes phosphatase activity. 2 Publications1
Mutagenesisi507T → A: Abrogates 14-3-3 protein binding; increases binding to cyclin B1. 1 Publication1
Mutagenesisi513S → A: Increased stability following IR treatment. 1 Publication1
Mutagenesisi513S → D: Mimicks phosphorylation state, leading to promote degradation following IR treatment. 1 Publication1
Mutagenesisi514K → L: Abrogates binding to CCNB1; when associated with L-520. 1 Publication1
Mutagenesisi519S → A: Increased stability following IR treatment. 1 Publication1
Mutagenesisi519S → D: Mimicks phosphorylation state, leading to promote degradation following IR treatment. 1 Publication1
Mutagenesisi520R → L: Abrogates binding to CCNB1; when associated with L-514. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		993

Open Targets

More...OpenTargetsi		ENSG00000164045

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA26259

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P30304, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL3775

DrugCentral

More...DrugCentrali		P30304

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		CDC25A

Domain mapping of disease mutations (DMDM)

More...DMDMi		50403734

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001986411 – 524M-phase inducer phosphatase 1Add BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei76Phosphoserine; by CHEK12 Publications1
Modified residuei79Phosphoserine; by NEK111 Publication1
Modified residuei82Phosphoserine; by NEK112 Publications1
Modified residuei88Phosphoserine; by NEK112 Publications1
Modified residuei107PhosphoserineCombined sources1
Modified residuei124Phosphoserine; by CHEK1 and CHEK25 Publications1
Modified residuei178Phosphoserine; by CHEK13 Publications1
Modified residuei279Phosphoserine; by CHEK1 and CHEK21 Publication1
Modified residuei293Phosphoserine; by CHEK1 and CHEK21 Publication1
Modified residuei321PhosphoserineCombined sources1
Modified residuei507Phosphothreonine; by CHEK11 Publication1
Modified residuei513Phosphoserine; by PLK31 Publication1
Modified residuei519Phosphoserine; by PLK31 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.11 Publications
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P30304

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P30304

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P30304

MaxQB - The MaxQuant DataBase

More...MaxQBi		P30304

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P30304

PeptideAtlas

More...PeptideAtlasi		P30304

PRoteomics IDEntifications database

More...PRIDEi		P30304

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		54652 [P30304-1]
54653 [P30304-2]

PTM databases

DEPOD human dephosphorylation database

More...DEPODi		CDC25A

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P30304

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P30304

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000164045, Expressed in secondary oocyte and 149 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P30304, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P30304, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000164045, Tissue enhanced (bone marrow, lymphoid tissue, testis)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with CCNB1/cyclin B1.

Interacts with YWHAE/14-3-3 epsilon when phosphorylated.

Interacts with CUL1 specifically when CUL1 is neddylated and active.

Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage.

Interacts with PIM1.

Interacts with CHEK2; mediates CDC25A phosphorylation and degradation in response to infrared-induced DNA damages.

Interacts with HSP90AB1; prevents heat shock-mediated CDC25A degradation and contributes to cell cycle progression (PubMed:22843495).

5 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		107428, 121 interactors

Database of interacting proteins

More...DIPi		DIP-166N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

More...ELMi		P30304

Protein interaction database and analysis system

More...IntActi		P30304, 46 interactors

Molecular INTeraction database

More...MINTi		P30304

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000303706

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P30304

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P30304, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1524
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P30304

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P30304

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini376 – 482RhodanesePROSITE-ProRule annotationAdd BLAST107

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi74 – 84PhosphodegronAdd BLAST11
Motifi141 – 143KEN box3

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG3772, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000160737

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_014464_0_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P30304

KEGG Orthology (KO)

More...KOi		K06645

Identification of Orthologs from Complete Genome Data

More...OMAi		GRTTQKR

Database for complete collections of gene phylogenies

More...PhylomeDBi		P30304

TreeFam database of animal gene trees

More...TreeFami		TF101056

Family and domain databases

Conserved Domains Database

More...CDDi		cd01530, Cdc25, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.250.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR000751, MPI_Phosphatase
IPR001763, Rhodanese-like_dom
IPR036873, Rhodanese-like_dom_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF06617, M-inducer_phosp, 1 hit
PF00581, Rhodanese, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00716, MPIPHPHTASE

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00450, RHOD, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF52821, SSF52821, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50206, RHODANESE_3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P30304-1) [UniParc]FASTAAdd to basket
Also known as: CDC25A1

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELGPEPPHR RRLLFACSPP PASQPVVKAL FGASAAGGLS PVTNLTVTMD 
        60         70         80         90        100
QLQGLGSDYE QPLEVKNNSN LQRMGSSEST DSGFCLDSPG PLDSKENLEN 
       110        120        130        140        150
PMRRIHSLPQ KLLGCSPALK RSHSDSLDHD IFQLIDPDEN KENEAFEFKK 
       160        170        180        190        200
PVRPVSRGCL HSHGLQEGKD LFTQRQNSAP ARMLSSNERD SSEPGNFIPL 
       210        220        230        240        250
FTPQSPVTAT LSDEDDGFVD LLDGENLKNE EETPSCMASL WTAPLVMRTT 
       260        270        280        290        300
NLDNRCKLFD SPSLCSSSTR SVLKRPERSQ EESPPGSTKR RKSMSGASPK 
       310        320        330        340        350
ESTNPEKAHE TLHQSLSLAS SPKGTIENIL DNDPRDLIGD FSKGYLFHTV 
       360        370        380        390        400
AGKHQDLKYI SPEIMASVLN GKFANLIKEF VIIDCRYPYE YEGGHIKGAV 
       410        420        430        440        450
NLHMEEEVED FLLKKPIVPT DGKRVIVVFH CEFSSERGPR MCRYVRERDR 
       460        470        480        490        500
LGNEYPKLHY PELYVLKGGY KEFFMKCQSY CEPPSYRPMH HEDFKEDLKK 
       510        520 
FRTKSRTWAG EKSKREMYSR LKKL
Length:524
Mass (Da):59,087
Last modified:July 19, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB2F6B792D4E6122B
GO
Isoform 2 (identifier: P30304-2) [UniParc]FASTAAdd to basket
Also known as: CDC25A2

The sequence of this isoform differs from the canonical sequence as follows:
     144-183: Missing.

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Length:484
Mass (Da):54,551
Checksum:iAE6326F34B704D4B
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JH94C9JH94_HUMAN
M-phase inducer phosphatase 1
CDC25A
143Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
C9JFR5C9JFR5_HUMAN
M-phase inducer phosphatase 1
CDC25A
252Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti6 – 10EPPHR → SPAP in AAA58415 (PubMed:1836978).Curated5
Sequence conflicti180 – 181PA → QL in AAA58415 (PubMed:1836978).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_02093288S → F1 PublicationCorresponds to variant dbSNP:rs3731499Ensembl.1
Natural variantiVAR_023532182R → G1 PublicationCorresponds to variant dbSNP:rs6771386Ensembl.1
Natural variantiVAR_023533182R → W. Corresponds to variant dbSNP:rs6771386Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_000860144 – 183Missing in isoform 2. 2 PublicationsAdd BLAST40

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
M81933 mRNA Translation: AAA58415.1
AY137580 mRNA Translation: AAN11305.1
AF527417 Genomic DNA Translation: AAM77917.1
BC007401 mRNA Translation: AAH07401.1
BC018642 mRNA Translation: AAH18642.1
AF277722 mRNA Translation: AAG41884.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS2760.1 [P30304-1]
CCDS2761.1 [P30304-2]

Protein sequence database of the Protein Information Resource

More...PIRi		A41648

NCBI Reference Sequences

More...RefSeqi		NP_001780.2, NM_001789.2 [P30304-1]
NP_963861.1, NM_201567.1 [P30304-2]
XP_011532618.1, XM_011534316.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000302506; ENSP00000303706; ENSG00000164045 [P30304-1]
ENST00000351231; ENSP00000343166; ENSG00000164045 [P30304-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		993

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:993

UCSC genome browser

More...UCSCi		uc003csh.2, human [P30304-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81933 mRNA Translation: AAA58415.1
AY137580 mRNA Translation: AAN11305.1
AF527417 Genomic DNA Translation: AAM77917.1
BC007401 mRNA Translation: AAH07401.1
BC018642 mRNA Translation: AAH18642.1
AF277722 mRNA Translation: AAG41884.1
CCDSiCCDS2760.1 [P30304-1]
CCDS2761.1 [P30304-2]
PIRiA41648
RefSeqiNP_001780.2, NM_001789.2 [P30304-1]
NP_963861.1, NM_201567.1 [P30304-2]
XP_011532618.1, XM_011534316.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C25X-ray2.30A337-496[»]
SMRiP30304
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi107428, 121 interactors
DIPiDIP-166N
ELMiP30304
IntActiP30304, 46 interactors
MINTiP30304
STRINGi9606.ENSP00000303706

Chemistry databases

BindingDBiP30304
ChEMBLiCHEMBL3775
DrugCentraliP30304

PTM databases

DEPODiCDC25A
iPTMnetiP30304
PhosphoSitePlusiP30304

Polymorphism and mutation databases

BioMutaiCDC25A
DMDMi50403734

Proteomic databases

EPDiP30304
jPOSTiP30304
MassIVEiP30304
MaxQBiP30304
PaxDbiP30304
PeptideAtlasiP30304
PRIDEiP30304
ProteomicsDBi54652 [P30304-1]
54653 [P30304-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		1619, 1664 antibodies

The DNASU plasmid repository

More...DNASUi		993

Genome annotation databases

EnsembliENST00000302506; ENSP00000303706; ENSG00000164045 [P30304-1]
ENST00000351231; ENSP00000343166; ENSG00000164045 [P30304-2]
GeneIDi993
KEGGihsa:993
UCSCiuc003csh.2, human [P30304-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		993
DisGeNETi993
EuPathDBiHostDB:ENSG00000164045.11

GeneCards: human genes, protein and diseases

More...GeneCardsi		CDC25A
HGNCiHGNC:1725, CDC25A
HPAiENSG00000164045, Tissue enhanced (bone marrow, lymphoid tissue, testis)
MIMi116947, gene
neXtProtiNX_P30304
OpenTargetsiENSG00000164045
PharmGKBiPA26259

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG3772, Eukaryota
GeneTreeiENSGT00940000160737
HOGENOMiCLU_014464_0_1_1
InParanoidiP30304
KOiK06645
OMAiGRTTQKR
PhylomeDBiP30304
TreeFamiTF101056

Enzyme and pathway databases

BRENDAi3.1.3.48, 2681
PathwayCommonsiP30304
ReactomeiR-HSA-1362277, Transcription of E2F targets under negative control by DREAM complex
R-HSA-156711, Polo-like kinase mediated events
R-HSA-176187, Activation of ATR in response to replication stress
R-HSA-5689880, Ub-specific processing proteases
R-HSA-69202, Cyclin E associated events during G1/S transition
R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition
R-HSA-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-69656, Cyclin A:Cdk2-associated events at S phase entry
R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models
SIGNORiP30304

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		993, 49 hits in 876 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		CDC25A, human
EvolutionaryTraceiP30304

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		CDC25A

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		993
PharosiP30304, Tchem

Protein Ontology

More...PROi		PR:P30304
RNActiP30304, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000164045, Expressed in secondary oocyte and 149 other tissues
ExpressionAtlasiP30304, baseline and differential
GenevisibleiP30304, HS

Family and domain databases

CDDicd01530, Cdc25, 1 hit
Gene3Di3.40.250.10, 1 hit
InterProiView protein in InterPro
IPR000751, MPI_Phosphatase
IPR001763, Rhodanese-like_dom
IPR036873, Rhodanese-like_dom_sf
PfamiView protein in Pfam
PF06617, M-inducer_phosp, 1 hit
PF00581, Rhodanese, 1 hit
PRINTSiPR00716, MPIPHPHTASE
SMARTiView protein in SMART
SM00450, RHOD, 1 hit
SUPFAMiSSF52821, SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206, RHODANESE_3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMPIP1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P30304
Secondary accession number(s): Q8IZH5, Q96IL3, Q9H2F2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 19, 2004
Last modified: August 12, 2020
This is version 196 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families
  6. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
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