WEE1

Functionⁱ

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Catalytic activityⁱ

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactorⁱ

Mg²⁺Note: Binds 2 magnesium ions per subunit.

Activity regulationⁱ

Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	328	ATPPROSITE-ProRule annotation	1
Active siteⁱ	426	Proton acceptorPROSITE-ProRule annotation	1
Metal bindingⁱ	431	Magnesium; via carbonyl oxygen1 Publication	1
Metal bindingⁱ	463	Magnesium; via carbonyl oxygen1 Publication	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	305 – 313	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
kinase activity Source: Reactome
magnesium ion binding Source: InterPro
non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
protein tyrosine kinase activity
Source: ProtInc
Traceable author statementⁱ
- 7774574

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell division Source: UniProtKB-KW
establishment of cell polarity Source: Ensembl
G2/M transition of mitotic cell cycle Source: Reactome
microtubule cytoskeleton organization Source: Ensembl
negative regulation of G1/S transition of mitotic cell cycle Source: Reactome
neuron projection morphogenesis Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase, Tyrosine-protein kinase
Biological process	Cell cycle, Cell division, Mitosis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.10.2 2681
Reactomeⁱ	R-HSA-156711 Polo-like kinase mediated events R-HSA-69202 Cyclin E associated events during G1/S transition R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69478 G2/M DNA replication checkpoint R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry R-HSA-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkⁱ	P30291
SIGNORⁱ	P30291

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Wee1-like protein kinase (EC:2.7.10.2) Short name: WEE1hu Alternative name(s): Wee1A kinase
Gene namesⁱ	Name:WEE1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 11

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000166483.10
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:12761 WEE1
MIMⁱ	193525 gene
neXtProtⁱ	NX_P30291

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	53	S → A: Abolishes phosphorylation by PLK1 and CDK1 and binding of the SCF(BTRC) complex, leading to stabilization of the protein; when associated with A-123. 1 Publication	1
Mutagenesisⁱ	116 – 117	EE → AA: Impairs binding of the SCF(BTRC) complex. 1 Publication	2
Mutagenesisⁱ	123	S → A: Abolishes phosphorylation by PLK1 and CDK1 and binding of the SCF(BTRC) complex, leading to stabilization of the protein; when associated with A-53. 1 Publication	1
Mutagenesisⁱ	328	K → R: Abolishes activity. 1 Publication	1
Mutagenesisⁱ	642	S → A: Abolishes phosphorylation by BRSK1 and BRSK2. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	7465
Open Targets More...OpenTargetsⁱ	ENSG00000166483
PharmGKBⁱ	PA366

Chemistry databases

ChEMBLⁱ	CHEMBL5491
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2278

Polymorphism and mutation databases

BioMutaⁱ	WEE1
DMDMⁱ	1351419

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086810	1 – 646	Wee1-like protein kinaseAdd BLAST		646

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	53	Phosphoserine; by PLK11 Publication	1
Modified residueⁱ	78	PhosphoserineBy similarity	1
Modified residueⁱ	85	PhosphoserineCombined sources	1
Modified residueⁱ	123	Phosphoserine; by CDK11 Publication	1
Modified residueⁱ	127	PhosphoserineCombined sources	1
Modified residueⁱ	137	PhosphoserineCombined sources	1
Modified residueⁱ	139	PhosphoserineCombined sources	1
Modified residueⁱ	150	PhosphoserineCombined sources	1
Modified residueⁱ	165	PhosphoserineBy similarity	1
Modified residueⁱ	187	PhosphothreonineCombined sources	1
Modified residueⁱ	190	PhosphothreonineCombined sources	1
Modified residueⁱ	239	Phosphothreonine1 Publication	1
Modified residueⁱ	270	PhosphoserineCombined sources	1
Modified residueⁱ	307	PhosphoserineCombined sources	1
Modified residueⁱ	312	PhosphoserineCombined sources	1
Modified residueⁱ	642	Phosphoserine; by BRSK1 and BRSK22 Publications	1

Post-translational modificationⁱ

Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.4 Publications

Dephosphorylated at Thr-239 by CTDP1.

Ubiquitinated and degraded at the onset of G2/M phase.1 Publication

Keywords - PTMⁱ

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	P30291
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P30291
PaxDbⁱ	P30291
PeptideAtlas More...PeptideAtlasⁱ	P30291
PRIDEⁱ	P30291
ProteomicsDBⁱ	54650

PTM databases

iPTMnetⁱ	P30291
PhosphoSitePlusⁱ	P30291

Miscellaneous databases

PMAP-CutDBⁱ	P30291

PMAP-CutDBⁱ

P30291

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000166483 Expressed in 203 organ(s), highest expression level in female gonad
CleanExⁱ	HS_WEE1
ExpressionAtlasⁱ	P30291 baseline and differential
Genevisibleⁱ	P30291 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB004619 HPA068845

HPAⁱ

CAB004619
HPA068845

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
BTRC	Q9Y297	2	EBI-914695,EBI-307461
FBXW11	Q9UKB1	3	EBI-914695,EBI-355189
YWHAZ	P63104	3	EBI-914695,EBI-347088

Protein-protein interaction databases

BioGridⁱ	113303, 76 interactors
CORUMⁱ	P30291
Database of interacting proteins More...DIPⁱ	DIP-37969N
IntActⁱ	P30291, 21 interactors
Molecular INTeraction database More...MINTⁱ	P30291
STRINGⁱ	9606.ENSP00000402084

Chemistry databases

BindingDBⁱ

P30291

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	294 – 298	Combined sources	5
Beta strandⁱ	299 – 308	Combined sources	10
Beta strandⁱ	311 – 318	Combined sources	8
Turnⁱ	319 – 321	Combined sources	3
Beta strandⁱ	324 – 331	Combined sources	8
Beta strandⁱ	335 – 337	Combined sources	3
Helixⁱ	338 – 352	Combined sources	15
Beta strandⁱ	362 – 368	Combined sources	7
Beta strandⁱ	371 – 377	Combined sources	7
Helixⁱ	384 – 394	Combined sources	11
Helixⁱ	400 – 419	Combined sources	20
Helixⁱ	429 – 431	Combined sources	3
Beta strandⁱ	432 – 435	Combined sources	4
Beta strandⁱ	458 – 461	Combined sources	4
Helixⁱ	464 – 466	Combined sources	3
Beta strandⁱ	468 – 472	Combined sources	5
Helixⁱ	480 – 482	Combined sources	3
Helixⁱ	485 – 488	Combined sources	4
Helixⁱ	495 – 510	Combined sources	16
Beta strandⁱ	518 – 520	Combined sources	3
Helixⁱ	521 – 527	Combined sources	7
Helixⁱ	540 – 549	Combined sources	10
Helixⁱ	554 – 556	Combined sources	3
Helixⁱ	560 – 564	Combined sources	5
Turnⁱ	567 – 569	Combined sources	3

Show more details

3D structure databases

Database of protein disorder More...DisProtⁱ	DP00611
ProteinModelPortalⁱ	P30291
SMRⁱ	P30291
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P30291

EvolutionaryTraceⁱ

P30291

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	299 – 569	Protein kinasePROSITE-ProRule annotationAdd BLAST		271

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	9 – 12	Poly-Pro		4
Compositional biasⁱ	34 – 43	Poly-Glu		10

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG0601 Eukaryota ENOG410XS1M LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063230
HOGENOMⁱ	HOG000004824
HOVERGENⁱ	HBG005050
InParanoidⁱ	P30291
KEGG Orthology (KO) More...KOⁱ	K06632
OMAⁱ	WNDSCGE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G049H
PhylomeDBⁱ	P30291
TreeFamⁱ	TF101088

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS IPR017164 Wee1-like_protein_kinase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
PIRSFⁱ	PIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: P30291-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFLSRQQPP PPRRAGAACT LRQKLIFSPC SDCEEEEEEE EEEGSGHSTG 
        60         70         80         90        100
EDSAFQEPDS PLPPARSPTE PGPERRRSPG PAPGSPGELE EDLLLPGACP 
       110        120        130        140        150
GADEAGGGAE GDSWEEEGFG SSSPVKSPAA PYFLGSSFSP VRCGGPGDAS 
       160        170        180        190        200
PRGCGARRAG EGRRSPRPDH PGTPPHKTFR KLRLFDTPHT PKSLLSKARG 
       210        220        230        240        250
IDSSSVKLRG SSLFMDTEKS GKREFDVRQT PQVNINPFTP DSLLLHSSGQ 
       260        270        280        290        300
CRRRKRTYWN DSCGEDMEAS DYELEDETRP AKRITITESN MKSRYTTEFH 
       310        320        330        340        350
ELEKIGSGEF GSVFKCVKRL DGCIYAIKRS KKPLAGSVDE QNALREVYAH 
       360        370        380        390        400
AVLGQHSHVV RYFSAWAEDD HMLIQNEYCN GGSLADAISE NYRIMSYFKE 
       410        420        430        440        450
AELKDLLLQV GRGLRYIHSM SLVHMDIKPS NIFISRTSIP NAASEEGDED 
       460        470        480        490        500
DWASNKVMFK IGDLGHVTRI SSPQVEEGDS RFLANEVLQE NYTHLPKADI 
       510        520        530        540        550
FALALTVVCA AGAEPLPRNG DQWHEIRQGR LPRIPQVLSQ EFTELLKVMI 
       560        570        580        590        600
HPDPERRPSA MALVKHSVLL SASRKSAEQL RIELNAEKFK NSLLQKELKK 
       610        620        630        640 
AQMAKAAAEE RALFTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY

Length:646

Mass (Da):71,597

Last modified:February 1, 1996 - v2

Checksum:ⁱDB00623D304562A0

GO

Isoform 2 (identifier: P30291-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-214: Missing.

« Hide

        10         20         30         40         50
MDTEKSGKRE FDVRQTPQVN INPFTPDSLL LHSSGQCRRR KRTYWNDSCG 
        60         70         80         90        100
EDMEASDYEL EDETRPAKRI TITESNMKSR YTTEFHELEK IGSGEFGSVF 
       110        120        130        140        150
KCVKRLDGCI YAIKRSKKPL AGSVDEQNAL REVYAHAVLG QHSHVVRYFS 
       160        170        180        190        200
AWAEDDHMLI QNEYCNGGSL ADAISENYRI MSYFKEAELK DLLLQVGRGL 
       210        220        230        240        250
RYIHSMSLVH MDIKPSNIFI SRTSIPNAAS EEGDEDDWAS NKVMFKIGDL 
       260        270        280        290        300
GHVTRISSPQ VEEGDSRFLA NEVLQENYTH LPKADIFALA LTVVCAAGAE 
       310        320        330        340        350
PLPRNGDQWH EIRQGRLPRI PQVLSQEFTE LLKVMIHPDP ERRPSAMALV 
       360        370        380        390        400
KHSVLLSASR KSAEQLRIEL NAEKFKNSLL QKELKKAQMA KAAAEERALF 
       410        420        430 
TDRMATRSTT QSNRTSRLIG KKMNRSVSLT IY

Note: No experimental confirmation available.

Show »

Length:432

Mass (Da):49,072

Checksum:ⁱ48F8E9EC16E4184A

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

E9PRI3	E9PRI3_HUMAN	Wee1-like protein kinase Wee1-like protein kinase	WEE1	99	Annotation score:
E9PRU3	E9PRU3_HUMAN	Wee1-like protein kinase Wee1-like protein kinase	WEE1	214	Annotation score:
H0YEJ2	H0YEJ2_HUMAN	Wee1-like protein kinase Wee1-like protein kinase	WEE1	145	Annotation score:
E9PQ51	E9PQ51_HUMAN	Wee1-like protein kinase Wee1-like protein kinase	WEE1	162	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	65	A → E in CAA43979 (Ref. 6) Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_041302	210	G → C1 PublicationCorresponds to variant dbSNP:rs34412975Ensembl.		1
Natural variantⁱVAR_041303	472	S → I1 PublicationCorresponds to variant dbSNP:rs56411856Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_044959	1 – 214	Missing in isoform 2. 1 PublicationAdd BLAST		214

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U10564 mRNA Translation: AAB60401.1 AJ277546 Genomic DNA Translation: CAC14173.1 AK122837 mRNA Translation: BAG53753.1 AC011979 Genomic DNA No translation available. AC122179 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68586.1 CH471064 Genomic DNA Translation: EAW68587.1 CH471064 Genomic DNA Translation: EAW68588.1 X62048 mRNA Translation: CAA43979.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS44536.1 [P30291-2] CCDS7800.1 [P30291-1]
PIRⁱ	S55048
NCBI Reference Sequences More...RefSeqⁱ	NP_001137448.1, NM_001143976.1 [P30291-2] NP_003381.1, NM_003390.3 [P30291-1]
UniGeneⁱ	Hs.249441

Genome annotation databases

Ensemblⁱ	ENST00000299613; ENSP00000299613; ENSG00000166483 [P30291-2] ENST00000450114; ENSP00000402084; ENSG00000166483 [P30291-1]
GeneIDⁱ	7465
KEGGⁱ	hsa:7465
UCSC genome browser More...UCSCⁱ	uc001mhs.4 human [P30291-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P30291	Wee1-like protein kinase (Fragment)		HUMAN		214	UniRef100_P30291
Isoform 2 of Wee1-like protein kinase		HUMAN		432
UPI000181D0EF		HUMAN		285

Protein	Similar proteins		Species	Score	Length	Source
P30291	Wee1-like protein kinase (Fragment)		HUMAN		214	UniRef90_P30291
WEE1 G2 checkpoint kinase		CANLF		665
Wee1-like protein kinase (Fragment)		MEPME		30
Wee1-like protein kinase (Fragment)		ARCPU		30
Wee1 kinase (Fragment)		SHEEP		165
+108

Protein	Similar proteins		Species	Score	Length	Source
P30291	Wee1-like protein kinase 1-A		XENLA		571	UniRef50_P30291
Wee1-like protein kinase		RAT		646
Wee1-like protein kinase		MOUSE		646
Wee1-like protein kinase (Fragment)		HUMAN		214
WEE1 G2 checkpoint kinase		CANLF		665
+265

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U10564 mRNA Translation: AAB60401.1 AJ277546 Genomic DNA Translation: CAC14173.1 AK122837 mRNA Translation: BAG53753.1 AC011979 Genomic DNA No translation available. AC122179 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68586.1 CH471064 Genomic DNA Translation: EAW68587.1 CH471064 Genomic DNA Translation: EAW68588.1 X62048 mRNA Translation: CAA43979.1
CCDSⁱ	CCDS44536.1 [P30291-2] CCDS7800.1 [P30291-1]
PIRⁱ	S55048
RefSeqⁱ	NP_001137448.1, NM_001143976.1 [P30291-2] NP_003381.1, NM_003390.3 [P30291-1]
UniGeneⁱ	Hs.249441

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1X8B	X-ray	1.81	A	291-575	[»]
	2IN6	X-ray	1.90	A	291-575	[»]
	2IO6	X-ray	2.20	A	291-575	[»]
	2Z2W	X-ray	2.22	A	291-575	[»]
	3BI6	X-ray	2.20	A	291-575	[»]
	3BIZ	X-ray	2.20	A	291-575	[»]
	3CQE	X-ray	2.50	A	291-575	[»]
	3CR0	X-ray	2.30	A	291-575	[»]
	5V5Y	X-ray	1.90	A	291-575	[»]
	5VC3	X-ray	1.97	A	291-575	[»]
	5VC4	X-ray	2.10	A	291-575	[»]
	5VC5	X-ray	1.93	A	291-575	[»]
	5VC6	X-ray	2.00	A	291-575	[»]
	5VD2	X-ray	2.05	A	291-575	[»]
	5VD4	X-ray	2.02	A	291-575	[»]
	5VD5	X-ray	2.05	A	291-575	[»]
	5VD7	X-ray	2.08	A	291-575	[»]
	5VD8	X-ray	1.85	A	291-575	[»]
	5VD9	X-ray	1.87	A	291-575	[»]
	5VDA	X-ray	2.10	A	291-575	[»]
DisProtⁱ	DP00611
ProteinModelPortalⁱ	P30291
SMRⁱ	P30291
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	113303, 76 interactors
CORUMⁱ	P30291
DIPⁱ	DIP-37969N
IntActⁱ	P30291, 21 interactors
MINTⁱ	P30291
STRINGⁱ	9606.ENSP00000402084

Chemistry databases

BindingDBⁱ	P30291
ChEMBLⁱ	CHEMBL5491
GuidetoPHARMACOLOGYⁱ	2278

PTM databases

iPTMnetⁱ	P30291
PhosphoSitePlusⁱ	P30291

Polymorphism and mutation databases

BioMutaⁱ	WEE1
DMDMⁱ	1351419

Proteomic databases

EPDⁱ	P30291
MaxQBⁱ	P30291
PaxDbⁱ	P30291
PeptideAtlasⁱ	P30291
PRIDEⁱ	P30291
ProteomicsDBⁱ	54650

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	7465
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000299613; ENSP00000299613; ENSG00000166483 [P30291-2] ENST00000450114; ENSP00000402084; ENSG00000166483 [P30291-1]
GeneIDⁱ	7465
KEGGⁱ	hsa:7465
UCSCⁱ	uc001mhs.4 human [P30291-1]

Organism-specific databases

CTDⁱ	7465
DisGeNETⁱ	7465
EuPathDBⁱ	HostDB:ENSG00000166483.10
GeneCardsⁱ	WEE1
HGNCⁱ	HGNC:12761 WEE1
HPAⁱ	CAB004619 HPA068845
MIMⁱ	193525 gene
neXtProtⁱ	NX_P30291
OpenTargetsⁱ	ENSG00000166483
PharmGKBⁱ	PA366
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0601 Eukaryota ENOG410XS1M LUCA
GeneTreeⁱ	ENSGT00530000063230
HOGENOMⁱ	HOG000004824
HOVERGENⁱ	HBG005050
InParanoidⁱ	P30291
KOⁱ	K06632
OMAⁱ	WNDSCGE
OrthoDBⁱ	EOG091G049H
PhylomeDBⁱ	P30291
TreeFamⁱ	TF101088

Enzyme and pathway databases

BRENDAⁱ	2.7.10.2 2681
Reactomeⁱ	R-HSA-156711 Polo-like kinase mediated events R-HSA-69202 Cyclin E associated events during G1/S transition R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition R-HSA-69478 G2/M DNA replication checkpoint R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry R-HSA-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkⁱ	P30291
SIGNORⁱ	P30291

Miscellaneous databases

ChiTaRSⁱ	WEE1 human
EvolutionaryTraceⁱ	P30291
GeneWikiⁱ	Wee1-like_protein_kinase
GenomeRNAiⁱ	7465
PMAP-CutDBⁱ	P30291
Protein Ontology More...PROⁱ	PR:P30291
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000166483 Expressed in 203 organ(s), highest expression level in female gonad
CleanExⁱ	HS_WEE1
ExpressionAtlasⁱ	P30291 baseline and differential
Genevisibleⁱ	P30291 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS IPR017164 Wee1-like_protein_kinase
Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
PIRSFⁱ	PIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	WEE1_HUMAN
Accessionⁱ	P30291Primary (citable) accession number: P30291 Secondary accession number(s): B3KVE1, D3DQV0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
	Last sequence update:	February 1, 1996
	Last modified:	September 12, 2018
	This is version 196 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human and mouse protein kinases
Human and mouse protein kinases: classification and index
SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - P30291 (WEE1_HUMAN)

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Wee1-like protein kinase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

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<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ