UniProtKB - P30282 (CCND3_MOUSE)
G1/S-specific cyclin-D3
Ccnd3
Functioni
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4.
By similarityGO - Molecular functioni
- cyclin-dependent protein serine/threonine kinase activity Source: MGI
- cyclin-dependent protein serine/threonine kinase regulator activity Source: GO_Central
- protein kinase binding Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- hyaluronan biosynthetic process Source: MGI
- mitotic cell cycle phase transition Source: GO_Central
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
- positive regulation of G1/S transition of mitotic cell cycle Source: GO_Central
- positive regulation of protein phosphorylation Source: MGI
- regulation of cell cycle Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
- regulation of insulin receptor signaling pathway Source: MGI
- response to peptide hormone Source: MGI
- signal transduction Source: MGI
- T cell proliferation Source: MGI
Keywordsi
Molecular function | Cyclin |
Biological process | Cell cycle, Cell division, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-69231, Cyclin D associated events in G1 R-MMU-8934593, Regulation of RUNX1 Expression and Activity |
Names & Taxonomyi
Protein namesi | Recommended name: G1/S-specific cyclin-D31 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88315, Ccnd3 |
VEuPathDBi | HostDB:ENSMUSG00000034165 |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cyclin D3-CDK4 complex Source: Ensembl
- cyclin-dependent protein kinase holoenzyme complex Source: MGI
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080443 | 1 – 292 | G1/S-specific cyclin-D3Add BLAST | 292 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 264 | PhosphoserineCombined sources | 1 | |
Modified residuei | 279 | PhosphoserineBy similarity | 1 | |
Modified residuei | 283 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P30282 |
jPOSTi | P30282 |
PaxDbi | P30282 |
PeptideAtlasi | P30282 |
PRIDEi | P30282 |
ProteomicsDBi | 281251 |
PTM databases
iPTMneti | P30282 |
PhosphoSitePlusi | P30282 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034165, Expressed in thymus and 321 other tissues |
ExpressionAtlasi | P30282, baseline and differential |
Genevisiblei | P30282, MM |
Interactioni
Subunit structurei
Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
Interacts with ATF5.
Interacts with EIF3K.
Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either CDKN1A or CDKN2A.
By similarityBinary interactionsi
P30282
With | #Exp. | IntAct |
---|---|---|
Cdk6 [Q64261] | 2 | EBI-847337,EBI-847380 |
GO - Molecular functioni
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198550, 12 interactors |
ComplexPortali | CPX-2077, Cyclin D3-CDK4 complex CPX-2079, Cyclin D3-CDK6 complex |
IntActi | P30282, 6 interactors |
STRINGi | 10090.ENSMUSP00000040488 |
Miscellaneous databases
RNActi | P30282, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 152 | Cyclin N-terminalAdd BLAST | 126 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 256 – 292 | DisorderedSequence analysisAdd BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 267 – 292 | Polar residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0656, Eukaryota |
GeneTreei | ENSGT00940000160743 |
InParanoidi | P30282 |
OMAi | TETIFMQ |
OrthoDBi | 1234739at2759 |
PhylomeDBi | P30282 |
TreeFami | TF101004 |
Family and domain databases
CDDi | cd00043, CYCLIN, 1 hit |
InterProi | View protein in InterPro IPR039361, Cyclin IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR015451, Cyclin_D IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit PTHR10177:SF65, PTHR10177:SF65, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 1 hit SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 11 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQK
60 70 80 90 100
EIKPHMRKML AYWMLEVCEE QRCEEDVFPL AMNYLDRYLS CVPTRKAQLQ
110 120 130 140 150
LLGTVCLLLA SKLRETTPLT IEKLCIYTDQ AVAPWQLREW EVLVLGKLKW
160 170 180 190 200
DLAAVIAHDF LALILHRLSL PSDRQALVKK HAQTFLALCA TDYTFAMYPP
210 220 230 240 250
SMIATGSIGA AVLGLGACSM SADELTELLA GITGTEVDCL RACQEQIEAA
260 270 280 290
LRESLREAAQ TAPSPVPKAP RGSSSQGPSQ TSTPTDVTAI HL
Computationally mapped potential isoform sequencesi
There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketS4R216 | S4R216_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 96 | Annotation score: | ||
S4R240 | S4R240_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 214 | Annotation score: | ||
S4R1D9 | S4R1D9_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 250 | Annotation score: | ||
S4R2N5 | S4R2N5_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 243 | Annotation score: | ||
S4R1C8 | S4R1C8_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 99 | Annotation score: | ||
S4R275 | S4R275_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 75 | Annotation score: | ||
S4R1R1 | S4R1R1_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 125 | Annotation score: | ||
S4R2I5 | S4R2I5_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 52 | Annotation score: | ||
S4R2N1 | S4R2N1_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 119 | Annotation score: | ||
S4R1L8 | S4R1L8_MOUSE | G1/S-specific cyclin-D3 | Ccnd3 | 43 | Annotation score: | ||
There is more potential isoformShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 239 | C → Y in AAH04076 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86183 mRNA Translation: AAA37504.1 U43844 Genomic DNA Translation: AAC53363.1 BC004076 mRNA Translation: AAH04076.1 BC005605 mRNA Translation: AAH05605.1 |
CCDSi | CCDS28850.1 |
PIRi | C40035 |
RefSeqi | NP_001075104.1, NM_001081635.1 NP_001075105.1, NM_001081636.1 NP_031658.1, NM_007632.2 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86183 mRNA Translation: AAA37504.1 U43844 Genomic DNA Translation: AAC53363.1 BC004076 mRNA Translation: AAH04076.1 BC005605 mRNA Translation: AAH05605.1 |
CCDSi | CCDS28850.1 |
PIRi | C40035 |
RefSeqi | NP_001075104.1, NM_001081635.1 NP_001075105.1, NM_001081636.1 NP_031658.1, NM_007632.2 |
3D structure databases
SMRi | P30282 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198550, 12 interactors |
ComplexPortali | CPX-2077, Cyclin D3-CDK4 complex CPX-2079, Cyclin D3-CDK6 complex |
IntActi | P30282, 6 interactors |
STRINGi | 10090.ENSMUSP00000040488 |
PTM databases
iPTMneti | P30282 |
PhosphoSitePlusi | P30282 |
Proteomic databases
EPDi | P30282 |
jPOSTi | P30282 |
PaxDbi | P30282 |
PeptideAtlasi | P30282 |
PRIDEi | P30282 |
ProteomicsDBi | 281251 |
Protocols and materials databases
Antibodypediai | 3521, 841 antibodies from 43 providers |
DNASUi | 12445 |
Genome annotation databases
Organism-specific databases
CTDi | 896 |
MGIi | MGI:88315, Ccnd3 |
VEuPathDBi | HostDB:ENSMUSG00000034165 |
Phylogenomic databases
eggNOGi | KOG0656, Eukaryota |
GeneTreei | ENSGT00940000160743 |
InParanoidi | P30282 |
OMAi | TETIFMQ |
OrthoDBi | 1234739at2759 |
PhylomeDBi | P30282 |
TreeFami | TF101004 |
Enzyme and pathway databases
Reactomei | R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-69231, Cyclin D associated events in G1 R-MMU-8934593, Regulation of RUNX1 Expression and Activity |
Miscellaneous databases
BioGRID-ORCSi | 12445, 10 hits in 67 CRISPR screens |
ChiTaRSi | Ccnd3, mouse |
PROi | PR:P30282 |
RNActi | P30282, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034165, Expressed in thymus and 321 other tissues |
ExpressionAtlasi | P30282, baseline and differential |
Genevisiblei | P30282, MM |
Family and domain databases
CDDi | cd00043, CYCLIN, 1 hit |
InterProi | View protein in InterPro IPR039361, Cyclin IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR015451, Cyclin_D IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit PTHR10177:SF65, PTHR10177:SF65, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 1 hit SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CCND3_MOUSE | |
Accessioni | P30282Primary (citable) accession number: P30282 Secondary accession number(s): Q62391, Q99KP2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families