UniProtKB - P30139 (THIG_ECOLI)
Protein
Thiazole synthase
Gene
thiG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.1 Publication
Catalytic activityi
- 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H+ + 2 H2OEC:2.8.1.10
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 95 | Schiff-base intermediate with DXPBy similarity | 1 | |
Binding sitei | 156 | DXP; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- sulfurtransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- thiamine biosynthetic process Source: EcoCyc
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Thiamine biosynthesis |
Ligand | Schiff base |
Enzyme and pathway databases
BioCyci | EcoCyc:THIG-MONOMER MetaCyc:THIG-MONOMER |
BRENDAi | 2.8.1.10, 2026 |
UniPathwayi | UPA00060 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:thiG Ordered Locus Names:b3991, JW5549 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162815 | 1 – 256 | Thiazole synthaseAdd BLAST | 256 |
Proteomic databases
PaxDbi | P30139 |
PRIDEi | P30139 |
Interactioni
Subunit structurei
Homotetramer. Forms heterodimers with either ThiH or ThiS.
1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4262656, 16 interactors |
ComplexPortali | CPX-2135, ThiG-ThiH thiazole phosphate synthase complex |
DIPi | DIP-6868N |
IntActi | P30139, 10 interactors |
STRINGi | 511145.b3991 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 182 – 183 | DXP bindingBy similarity | 2 | |
Regioni | 204 – 205 | DXP bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the ThiG family.Curated
Phylogenomic databases
eggNOGi | COG2022, Bacteria |
HOGENOMi | CLU_062233_1_0_6 |
InParanoidi | P30139 |
PhylomeDBi | P30139 |
Family and domain databases
CDDi | cd04728, ThiG, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00443, ThiG, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR033983, Thiazole_synthase_ThiG IPR008867, ThiG |
PANTHERi | PTHR34266, PTHR34266, 1 hit |
Pfami | View protein in Pfam PF05690, ThiG, 1 hit |
i Sequence
Sequence statusi: Complete.
P30139-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRIADKTFD SHLFTGTGKF ASSQLMVEAI RASGSQLVTL AMKRVDLRQH
60 70 80 90 100
NDAILEPLIA AGVTLLPNTS GAKTAEEAIF AAHLAREALG TNWLKLEIHP
110 120 130 140 150
DARWLLPDPI ETLKAAETLV QQGFVVLPYC GADPVLCKRL EEVGCAAVMP
160 170 180 190 200
LGAPIGSNQG LETRAMLEII IQQATVPVVV DAGIGVPSHA AQALEMGADA
210 220 230 240 250
VLVNTAIAVA DDPVNMAKAF RLAVEAGLLA RQSGPGSRSY FAHATSPLTG
FLEASA
Sequence cautioni
The sequence AAC43089 differs from that shown. Reason: Erroneous initiation.Curated
Mass spectrometryi
Molecular mass is 26893.3 Da. Determined by ESI. 1 Publication
Molecular mass is 26896.5 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88701 Genomic DNA Translation: AAB95621.1 U00006 Genomic DNA Translation: AAC43089.1 Different initiation. U00096 Genomic DNA Translation: AAC76965.2 AP009048 Genomic DNA Translation: BAE77329.1 |
PIRi | B65206 |
RefSeqi | NP_418418.2, NC_000913.3 WP_000944104.1, NZ_SSZK01000047.1 |
Genome annotation databases
EnsemblBacteriai | AAC76965; AAC76965; b3991 BAE77329; BAE77329; BAE77329 |
GeneIDi | 948493 |
KEGGi | ecj:JW5549 eco:b3991 |
PATRICi | fig|1411691.4.peg.2721 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88701 Genomic DNA Translation: AAB95621.1 U00006 Genomic DNA Translation: AAC43089.1 Different initiation. U00096 Genomic DNA Translation: AAC76965.2 AP009048 Genomic DNA Translation: BAE77329.1 |
PIRi | B65206 |
RefSeqi | NP_418418.2, NC_000913.3 WP_000944104.1, NZ_SSZK01000047.1 |
3D structure databases
SMRi | P30139 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262656, 16 interactors |
ComplexPortali | CPX-2135, ThiG-ThiH thiazole phosphate synthase complex |
DIPi | DIP-6868N |
IntActi | P30139, 10 interactors |
STRINGi | 511145.b3991 |
Proteomic databases
PaxDbi | P30139 |
PRIDEi | P30139 |
Genome annotation databases
EnsemblBacteriai | AAC76965; AAC76965; b3991 BAE77329; BAE77329; BAE77329 |
GeneIDi | 948493 |
KEGGi | ecj:JW5549 eco:b3991 |
PATRICi | fig|1411691.4.peg.2721 |
Organism-specific databases
EchoBASEi | EB1547 |
Phylogenomic databases
eggNOGi | COG2022, Bacteria |
HOGENOMi | CLU_062233_1_0_6 |
InParanoidi | P30139 |
PhylomeDBi | P30139 |
Enzyme and pathway databases
UniPathwayi | UPA00060 |
BioCyci | EcoCyc:THIG-MONOMER MetaCyc:THIG-MONOMER |
BRENDAi | 2.8.1.10, 2026 |
Miscellaneous databases
PROi | PR:P30139 |
Family and domain databases
CDDi | cd04728, ThiG, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00443, ThiG, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR033983, Thiazole_synthase_ThiG IPR008867, ThiG |
PANTHERi | PTHR34266, PTHR34266, 1 hit |
Pfami | View protein in Pfam PF05690, ThiG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THIG_ECOLI | |
Accessioni | P30139Primary (citable) accession number: P30139 Secondary accession number(s): P76779, Q2M8S7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | August 29, 2001 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families