UniProtKB - P30138 (THIF_ECOLI)
Protein
Sulfur carrier protein ThiS adenylyltransferase
Gene
thiF
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS.
Catalytic activityi
- [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + ATP + H+ = [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + diphosphate1 PublicationEC:2.7.7.731 Publication
Cofactori
Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.2 Publications
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 11 | ATP | 1 | |
Binding sitei | 38 | ATP; via amide nitrogen | 1 | |
Binding sitei | 59 | ATP | 1 | |
Binding sitei | 70 | ATP | 1 | |
Binding sitei | 83 | ATP | 1 | |
Binding sitei | 107 | ATP; via amide nitrogen | 1 | |
Metal bindingi | 169 | Zinc1 Publication | 1 | |
Metal bindingi | 172 | Zinc1 Publication | 1 | |
Active sitei | 184 | Glycyl persulfide ester intermediate | 1 | |
Metal bindingi | 240 | Zinc1 Publication | 1 | |
Metal bindingi | 243 | Zinc1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 127 – 131 | ATP | 5 |
GO - Molecular functioni
- ATP binding Source: EcoCyc
- magnesium ion binding Source: EcoCyc
- metal ion binding Source: EcoCyc
- nucleotidyltransferase activity Source: EcoCyc
- ubiquitin-like modifier activating enzyme activity Source: InterPro
- zinc ion binding Source: EcoCyc
GO - Biological processi
- thiamine biosynthetic process Source: UniProtKB-KW
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Thiamine biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:THIF-MONOMER MetaCyc:THIF-MONOMER |
BRENDAi | 2.7.7.73, 2026 |
UniPathwayi | UPA00060 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:thiF Ordered Locus Names:b3992, JW3956 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 174 | W → A: No adenylation of ThiS. 1 Publication | 1 | |
Mutagenesisi | 184 | C → S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120578 | 1 – 251 | Sulfur carrier protein ThiS adenylyltransferaseAdd BLAST | 251 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 184 | Glycyl cysteine dithioester (Cys-Gly) (interchain with G-Cter in ThiS) |
Keywords - PTMi
Thioester bondProteomic databases
PaxDbi | P30138 |
PRIDEi | P30138 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4262657, 10 interactors 852794, 2 interactors |
ComplexPortali | CPX-2134, ThiF-ThiS complex |
IntActi | P30138, 8 interactors |
STRINGi | 511145.b3992 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P30138 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30138 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 59 – 62 | Poly-Asp | 4 |
Sequence similaritiesi
Belongs to the HesA/MoeB/ThiF family.Curated
Phylogenomic databases
eggNOGi | COG0476, Bacteria |
HOGENOMi | CLU_013325_10_3_6 |
InParanoidi | P30138 |
PhylomeDBi | P30138 |
Family and domain databases
InterProi | View protein in InterPro IPR012731, Adenyl_ThiF IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
TIGRFAMsi | TIGR02356, adenyl_thiF, 1 hit |
i Sequence
Sequence statusi: Complete.
P30138-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNDRDFMRYS RQILLDDIAL DGQQKLLDSQ VLIIGLGGLG TPAALYLAGA
60 70 80 90 100
GVGTLVLADD DDVHLSNLQR QILFTTEDID RPKSQVSQQR LTQLNPDIQL
110 120 130 140 150
TALQQRLTGE ALKDAVARAD VVLDCTDNMA TRQEINAACV ALNTPLITAS
160 170 180 190 200
AVGFGGQLMV LTPPWEQGCY RCLWPDNQEP ERNCRTAGVV GPVVGVMGTL
210 220 230 240 250
QALEAIKLLS GIETPAGELR LFDGKSSQWR SLALRRASGC PVCGGSNADP
V
Sequence cautioni
The sequence AAC43090 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 229 – 230 | WR → C in AAB95618 (PubMed:8432721).Curated | 2 |
Mass spectrometryi
Molecular mass is 26970.2 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88701 Genomic DNA Translation: AAB95618.1 U00006 Genomic DNA Translation: AAC43090.1 Different initiation. U00096 Genomic DNA Translation: AAC76966.2 AP009048 Genomic DNA Translation: BAE77327.1 |
PIRi | C65206 |
RefSeqi | NP_418420.4, NC_000913.3 WP_000999737.1, NZ_STEB01000045.1 |
Genome annotation databases
EnsemblBacteriai | AAC76966; AAC76966; b3992 BAE77327; BAE77327; BAE77327 |
GeneIDi | 57730783 948500 |
KEGGi | ecj:JW3956 eco:b3992 |
PATRICi | fig|1411691.4.peg.2719 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88701 Genomic DNA Translation: AAB95618.1 U00006 Genomic DNA Translation: AAC43090.1 Different initiation. U00096 Genomic DNA Translation: AAC76966.2 AP009048 Genomic DNA Translation: BAE77327.1 |
PIRi | C65206 |
RefSeqi | NP_418420.4, NC_000913.3 WP_000999737.1, NZ_STEB01000045.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZFN | X-ray | 2.75 | A/B/C/D | 1-251 | [»] | |
1ZKM | X-ray | 2.95 | A/B/C/D | 1-251 | [»] | |
1ZUD | X-ray | 1.98 | 1/3 | 1-251 | [»] | |
SMRi | P30138 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262657, 10 interactors 852794, 2 interactors |
ComplexPortali | CPX-2134, ThiF-ThiS complex |
IntActi | P30138, 8 interactors |
STRINGi | 511145.b3992 |
Proteomic databases
PaxDbi | P30138 |
PRIDEi | P30138 |
Genome annotation databases
EnsemblBacteriai | AAC76966; AAC76966; b3992 BAE77327; BAE77327; BAE77327 |
GeneIDi | 57730783 948500 |
KEGGi | ecj:JW3956 eco:b3992 |
PATRICi | fig|1411691.4.peg.2719 |
Organism-specific databases
EchoBASEi | EB1546 |
Phylogenomic databases
eggNOGi | COG0476, Bacteria |
HOGENOMi | CLU_013325_10_3_6 |
InParanoidi | P30138 |
PhylomeDBi | P30138 |
Enzyme and pathway databases
UniPathwayi | UPA00060 |
BioCyci | EcoCyc:THIF-MONOMER MetaCyc:THIF-MONOMER |
BRENDAi | 2.7.7.73, 2026 |
Miscellaneous databases
EvolutionaryTracei | P30138 |
PROi | PR:P30138 |
Family and domain databases
InterProi | View protein in InterPro IPR012731, Adenyl_ThiF IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
TIGRFAMsi | TIGR02356, adenyl_thiF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THIF_ECOLI | |
Accessioni | P30138Primary (citable) accession number: P30138 Secondary accession number(s): P76780, Q2M8S9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families