UniProtKB - P30131 (HYPF_ECOLI)
Carbamoyltransferase HypF
hypF
Functioni
Involved in the maturation of [NiFe] hydrogenases (PubMed:8661925, PubMed:12377778, PubMed:12586941).
Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases (PubMed:12586941).
HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide (PubMed:12586941, PubMed:15291820).
In the absence of any other substrate, displays carbamoyl-phosphate phosphatase activity (PubMed:12377778).
4 PublicationsCatalytic activityi
- ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H+ + phosphate2 Publications
: [NiFe] hydrogenase maturation Pathwayi
This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 109 – 134 | C4-type1 PublicationAdd BLAST | 26 | |
Zinc fingeri | 159 – 184 | C4-type1 PublicationAdd BLAST | 26 |
GO - Molecular functioni
- carboxyl- or carbamoyltransferase activity Source: EcoCyc
- double-stranded RNA binding Source: InterPro
- ligase activity Source: UniProtKB-KW
- zinc ion binding Source: EcoCyc
GO - Biological processi
- peptidyl-S-carbamoyl-L-cysteine dehydration Source: ComplexPortal
- protein carbamoylation Source: EcoCyc
- protein maturation Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11551-MONOMER |
UniPathwayi | UPA00335 |
Names & Taxonomyi
Protein namesi | Recommended name: Carbamoyltransferase HypFCurated (EC:6.2.-.-2 Publications)Alternative name(s): Carbamoyl phosphate-converting enzyme HypF [NiFe]-hydrogenase maturation factor HypF Short name: Hydrogenase maturation protein HypF |
Gene namesi | Name:hypF1 Publication Synonyms:hydA1 Publication Ordered Locus Names:b2712, JW5433 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | R → E or H: Loss of activity. 2 Publications | 1 | |
Mutagenesisi | 23 | R → Q: Diminishes activity. 2 Publications | 1 | |
Mutagenesisi | 109 | C → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 159 | C → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 475 | H → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 476 | H → A: Carbamoyl phosphate-dependent ATP hydrolysis activity diminished in vitro but sufficient for wild-type-like phenotype. 2 Publications | 1 | |
Mutagenesisi | 479 | H → A: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000071614 | 1 – 750 | Carbamoyltransferase HypFAdd BLAST | 750 |
Proteomic databases
jPOSTi | P30131 |
PaxDbi | P30131 |
PRIDEi | P30131 |
Interactioni
Subunit structurei
Binary interactionsi
P30131
With | #Exp. | IntAct |
---|---|---|
hypE [P24193] | 2 | EBI-9128507,EBI-552743 |
Protein-protein interaction databases
BioGRIDi | 4259427, 14 interactors 849360, 8 interactors |
ComplexPortali | CPX-5281, HypEF Ni-hydrogenase maturation complex |
DIPi | DIP-10000N |
IntActi | P30131, 8 interactors |
STRINGi | 511145.b2712 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P30131 |
SMRi | P30131 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30131 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 92 | Acylphosphatase-likePROSITE-ProRule annotationAdd BLAST | 85 | |
Domaini | 200 – 376 | YrdC-likePROSITE-ProRule annotationAdd BLAST | 177 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 23 | Substrate binding1 Publication | 5 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 109 – 134 | C4-type1 PublicationAdd BLAST | 26 | |
Zinc fingeri | 159 – 184 | C4-type1 PublicationAdd BLAST | 26 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | COG0068, Bacteria |
HOGENOMi | CLU_009164_0_0_6 |
InParanoidi | P30131 |
OMAi | VQHHYAH |
PhylomeDBi | P30131 |
Family and domain databases
InterProi | View protein in InterPro IPR001792, Acylphosphatase-like_dom IPR036046, Acylphosphatase-like_dom_sf IPR017968, Acylphosphatase_CS IPR004421, Carbamoyltransferase_HypF IPR017945, DHBP_synth_RibB-like_a/b_dom IPR041440, HypF_C IPR006070, YrdC-like_dom IPR011125, Znf_HypF |
Pfami | View protein in Pfam PF00708, Acylphosphatase, 1 hit PF17788, HypF_C, 1 hit PF01300, Sua5_yciO_yrdC, 1 hit PF07503, zf-HYPF, 2 hits |
PIRSFi | PIRSF006256, CMPcnvr_hdrg_mat, 1 hit |
SUPFAMi | SSF54975, SSF54975, 1 hit SSF55821, SSF55821, 1 hit |
TIGRFAMsi | TIGR00143, hypF, 1 hit |
PROSITEi | View protein in PROSITE PS00150, ACYLPHOSPHATASE_1, 1 hit PS51160, ACYLPHOSPHATASE_3, 1 hit PS51163, YRDC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAKNTSCGVQ LRIRGKVQGV GFRPFVWQLA QQLNLHGDVC NDGDGVEVRL
60 70 80 90 100
REDPETFLVQ LYQHCPPLAR IDSVEREPFI WSQLPTEFTI RQSTGGTMNT
110 120 130 140 150
QIVPDAATCP ACLAEMNTPG ERRYRYPFIN CTHCGPRFTI IRAMPYDRPF
160 170 180 190 200
TVMAAFPLCP ACDKEYRDPL DRRFHAQPVA CPECGPHLEW VSHGEHAEQE
210 220 230 240 250
AALQAAIAQL KMGKIVAIKG IGGFHLACDA RNSNAVATLR ARKHRPAKPL
260 270 280 290 300
AVMLPVADGL PDAARQLLTT PAAPIVLVDK KYVPELCDDI APDLNEVGVM
310 320 330 340 350
LPANPLQHLL LQELQCPLVM TSGNLSGKPP AISNEQALAD LQGIADGFLI
360 370 380 390 400
HNRDIVQRMD DSVVRESGEM LRRSRGYVPD ALALPPGFKN VPPVLCLGAD
410 420 430 440 450
LKNTFCLVRG EQAVLSQHLG DLSDDGIQMQ WREALRLMQN IYDFTPQYVV
460 470 480 490 500
HDAHPGYVSS QWAREMNLPT QTVLHHHAHA AACLAEHQWP LDGGDVIALT
510 520 530 540 550
LDGIGMGENG ALWGGECLRV NYRECEHLGG LPAVALPGGD LAAKQPWRNL
560 570 580 590 600
LAQCLRFVPE WQNYSETASV QQQNWSVLAR AIERGINAPL ASSCGRFFDA
610 620 630 640 650
VAAALGCAPA TLSYEGEAAC ALEALAASCH GVTHPVTMPR VDNQLDLATF
660 670 680 690 700
WQQWLNWQAP VNQRAWAFHD ALAQGFAALM REQATMRGIT TLVFSGGVIH
710 720 730 740 750
NRLLRARLAH YLADFTLLFP QSLPAGDGGL SLGQGVIAAA RWLAGEVQNG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 – 32 | QQ → RE in BAA03315 (Ref. 1) Curated | 2 | |
Sequence conflicti | 367 – 386 | SGEML…LALPP → RRNAAPFAGVCAGCAGFAS in BAA03315 (Ref. 1) CuratedAdd BLAST | 20 | |
Sequence conflicti | 431 – 432 | WR → CA in BAA03315 (Ref. 1) Curated | 2 | |
Sequence conflicti | 435 | L → S in BAA03315 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14422 Genomic DNA Translation: BAA03315.1 U29579 Genomic DNA Translation: AAA69222.1 U00096 Genomic DNA Translation: AAC75754.1 AP009048 Genomic DNA Translation: BAE76789.1 |
PIRi | D65051 |
RefSeqi | NP_417192.1, NC_000913.3 WP_001107704.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75754; AAC75754; b2712 BAE76789; BAE76789; BAE76789 |
GeneIDi | 944963 |
KEGGi | ecj:JW5433 eco:b2712 |
PATRICi | fig|1411691.4.peg.4030 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14422 Genomic DNA Translation: BAA03315.1 U29579 Genomic DNA Translation: AAA69222.1 U00096 Genomic DNA Translation: AAC75754.1 AP009048 Genomic DNA Translation: BAE76789.1 |
PIRi | D65051 |
RefSeqi | NP_417192.1, NC_000913.3 WP_001107704.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GXT | X-ray | 1.30 | A | 1-91 | [»] | |
1GXU | X-ray | 1.27 | A | 1-91 | [»] | |
AlphaFoldDBi | P30131 | |||||
SMRi | P30131 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259427, 14 interactors 849360, 8 interactors |
ComplexPortali | CPX-5281, HypEF Ni-hydrogenase maturation complex |
DIPi | DIP-10000N |
IntActi | P30131, 8 interactors |
STRINGi | 511145.b2712 |
Proteomic databases
jPOSTi | P30131 |
PaxDbi | P30131 |
PRIDEi | P30131 |
Genome annotation databases
EnsemblBacteriai | AAC75754; AAC75754; b2712 BAE76789; BAE76789; BAE76789 |
GeneIDi | 944963 |
KEGGi | ecj:JW5433 eco:b2712 |
PATRICi | fig|1411691.4.peg.4030 |
Organism-specific databases
EchoBASEi | EB1512 |
Phylogenomic databases
eggNOGi | COG0068, Bacteria |
HOGENOMi | CLU_009164_0_0_6 |
InParanoidi | P30131 |
OMAi | VQHHYAH |
PhylomeDBi | P30131 |
Enzyme and pathway databases
UniPathwayi | UPA00335 |
BioCyci | EcoCyc:EG11551-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P30131 |
PROi | PR:P30131 |
Family and domain databases
InterProi | View protein in InterPro IPR001792, Acylphosphatase-like_dom IPR036046, Acylphosphatase-like_dom_sf IPR017968, Acylphosphatase_CS IPR004421, Carbamoyltransferase_HypF IPR017945, DHBP_synth_RibB-like_a/b_dom IPR041440, HypF_C IPR006070, YrdC-like_dom IPR011125, Znf_HypF |
Pfami | View protein in Pfam PF00708, Acylphosphatase, 1 hit PF17788, HypF_C, 1 hit PF01300, Sua5_yciO_yrdC, 1 hit PF07503, zf-HYPF, 2 hits |
PIRSFi | PIRSF006256, CMPcnvr_hdrg_mat, 1 hit |
SUPFAMi | SSF54975, SSF54975, 1 hit SSF55821, SSF55821, 1 hit |
TIGRFAMsi | TIGR00143, hypF, 1 hit |
PROSITEi | View protein in PROSITE PS00150, ACYLPHOSPHATASE_1, 1 hit PS51160, ACYLPHOSPHATASE_3, 1 hit PS51163, YRDC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HYPF_ECOLI | |
Accessioni | P30131Primary (citable) accession number: P30131 Secondary accession number(s): Q2MAB7, Q46878 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families