UniProtKB - P30115 (GSTA3_MOUSE)
Protein
Glutathione S-transferase A3
Gene
Gsta3
Organism
Mus musculus (Mouse)
Status
Functioni
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta5-androstene-3,17-dione and delta5-pregnene-3,20-dione, precursors to testosterone and progesterone, respectively (By similarity). Has a high catalytic activity for aflatoxin B1-8,9 epoxide (PubMed:1637297).By similarity1 Publication
Catalytic activityi
- EC:2.5.1.18By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 9 | GlutathioneBy similarity | 1 | |
Binding sitei | 45 | GlutathioneBy similarity | 1 |
GO - Molecular functioni
- glutathione transferase activity Source: MGI
GO - Biological processi
- aflatoxin catabolic process Source: MGI
- glutathione metabolic process Source: UniProtKB
- ureteric bud development Source: UniProtKB
- xenobiotic metabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-156590, Glutathione conjugation R-MMU-189483, Heme degradation |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione S-transferase A3Curated (EC:2.5.1.18)Alternative name(s): GST class-alpha member 3 Glutathione S-transferase Ya3 Glutathione S-transferase Yc |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95856, Gsta3 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000185790 | 2 – 221 | Glutathione S-transferase A3Add BLAST | 220 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 4 | N6-succinyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | non-CPTAC-3324 |
jPOSTi | P30115 |
MaxQBi | P30115 |
PaxDbi | P30115 |
PeptideAtlasi | P30115 |
PRIDEi | P30115 |
2D gel databases
SWISS-2DPAGEi | P30115 |
PTM databases
iPTMneti | P30115 |
PhosphoSitePlusi | P30115 |
SwissPalmi | P30115 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025934, Expressed in liver and 234 other tissues |
Genevisiblei | P30115, MM |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
IntActi | P30115, 1 interactor |
STRINGi | 10090.ENSMUSP00000027067 |
Miscellaneous databases
RNActi | P30115, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 83 | GST N-terminalAdd BLAST | 81 | |
Domaini | 85 – 207 | GST C-terminalAdd BLAST | 123 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 54 – 55 | Glutathione bindingBy similarity | 2 | |
Regioni | 67 – 68 | Glutathione bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1695, Eukaryota |
GeneTreei | ENSGT00940000163367 |
HOGENOMi | CLU_039475_4_0_1 |
InParanoidi | P30115 |
OMAi | RITNDEW |
OrthoDBi | 1162336at2759 |
PhylomeDBi | P30115 |
TreeFami | TF105321 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR003080, GST_alpha IPR004046, GST_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
PRINTSi | PR01266, GSTRNSFRASEA |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P30115-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA
110 120 130 140 150
DLEIMILYYP HMPPEEKEAS LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLSRADIAL VELLYHVEEL DPGVVDNFPL LKALRSRVSN LPTVKKFLQP
210 220
GSQRKPFDDA KCVESAKKIF S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WQI6 | A0A087WQI6_MOUSE | Glutathione S-transferase | Gsta3 | 133 | Annotation score: |
Mass spectrometryi
Molecular mass is 25271.4±2 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65021 mRNA Translation: CAA46155.1 M73483 mRNA Translation: AAA37751.1 AK014076 mRNA Translation: BAB29143.1 AK149543 mRNA Translation: BAE28948.1 CH466536 Genomic DNA Translation: EDL14389.1 CH466536 Genomic DNA Translation: EDL14390.1 BC147272 mRNA Translation: AAI47273.1 BC147273 mRNA Translation: AAI47274.1 |
CCDSi | CCDS14847.1 |
PIRi | S24322 |
RefSeqi | NP_001070821.1, NM_001077353.2 NP_034486.2, NM_010356.4 |
Genome annotation databases
Ensembli | ENSMUST00000027067; ENSMUSP00000027067; ENSMUSG00000025934 ENSMUST00000121676; ENSMUSP00000113262; ENSMUSG00000025934 |
GeneIDi | 14859 |
KEGGi | mmu:14859 |
UCSCi | uc007alk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65021 mRNA Translation: CAA46155.1 M73483 mRNA Translation: AAA37751.1 AK014076 mRNA Translation: BAB29143.1 AK149543 mRNA Translation: BAE28948.1 CH466536 Genomic DNA Translation: EDL14389.1 CH466536 Genomic DNA Translation: EDL14390.1 BC147272 mRNA Translation: AAI47273.1 BC147273 mRNA Translation: AAI47274.1 |
CCDSi | CCDS14847.1 |
PIRi | S24322 |
RefSeqi | NP_001070821.1, NM_001077353.2 NP_034486.2, NM_010356.4 |
3D structure databases
SMRi | P30115 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P30115, 1 interactor |
STRINGi | 10090.ENSMUSP00000027067 |
PTM databases
iPTMneti | P30115 |
PhosphoSitePlusi | P30115 |
SwissPalmi | P30115 |
2D gel databases
SWISS-2DPAGEi | P30115 |
Proteomic databases
CPTACi | non-CPTAC-3324 |
jPOSTi | P30115 |
MaxQBi | P30115 |
PaxDbi | P30115 |
PeptideAtlasi | P30115 |
PRIDEi | P30115 |
Protocols and materials databases
Antibodypediai | 30946, 68 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027067; ENSMUSP00000027067; ENSMUSG00000025934 ENSMUST00000121676; ENSMUSP00000113262; ENSMUSG00000025934 |
GeneIDi | 14859 |
KEGGi | mmu:14859 |
UCSCi | uc007alk.2, mouse |
Organism-specific databases
CTDi | 2940 |
MGIi | MGI:95856, Gsta3 |
Phylogenomic databases
eggNOGi | KOG1695, Eukaryota |
GeneTreei | ENSGT00940000163367 |
HOGENOMi | CLU_039475_4_0_1 |
InParanoidi | P30115 |
OMAi | RITNDEW |
OrthoDBi | 1162336at2759 |
PhylomeDBi | P30115 |
TreeFami | TF105321 |
Enzyme and pathway databases
Reactomei | R-MMU-156590, Glutathione conjugation R-MMU-189483, Heme degradation |
Miscellaneous databases
BioGRID-ORCSi | 14859, 1 hit in 18 CRISPR screens |
ChiTaRSi | Gsta3, mouse |
PROi | PR:P30115 |
RNActi | P30115, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025934, Expressed in liver and 234 other tissues |
Genevisiblei | P30115, MM |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR003080, GST_alpha IPR004046, GST_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
PRINTSi | PR01266, GSTRNSFRASEA |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSTA3_MOUSE | |
Accessioni | P30115Primary (citable) accession number: P30115 Secondary accession number(s): Q544Y6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families