UniProtKB - P30099 (C11B2_RAT)
Cytochrome P450 11B2, mitochondrial
Cyp11b2
Functioni
A cytochrome P450 monooxygenase that catalyzes the biosynthesis of adrenal mineralocorticoid aldosterone. Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone/21-hydroxyprogesterone, namely 11-beta hydroxylation followed with two successive oxidations at C18 to yield 18-hydroxy and then 18-aldehyde derivatives, resulting in the formation of aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
By similarityCatalytic activityi
- a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11β-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]By similarityEC:1.14.15.4By similarityThis reaction proceeds in the forwardBy similarity direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
- corticosterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxycorticosterone + H2O + 2 oxidized [adrenodoxin]By similarityEC:1.14.15.5By similarityThis reaction proceeds in the forwardBy similarity direction.
- 18-hydroxycorticosterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = aldosterone + 2 H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: Steroid biosynthesis Pathwayi
This protein is involved in Steroid biosynthesis.By similarityView all proteins of this organism that are known to be involved in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 391 | 11-deoxycorticosterone; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 457 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- corticosterone 18-monooxygenase activity Source: RGD
- heme binding Source: UniProtKB
- iron ion binding Source: InterPro
- steroid 11-beta-monooxygenase activity Source: RGD
GO - Biological processi
- aldosterone biosynthetic process Source: RGD
- C21-steroid hormone biosynthetic process Source: RGD
- cellular response to mechanical stimulus Source: RGD
- cellular response to nutrient Source: RGD
- cellular response to peptide hormone stimulus Source: RGD
- cellular response to potassium ion starvation Source: RGD
- cholesterol metabolic process Source: GO_Central
- cortisol metabolic process Source: GO_Central
- glucocorticoid biosynthetic process Source: RGD
- positive regulation of blood pressure Source: RGD
- response to insulin Source: RGD
- response to nutrient Source: RGD
- response to peptide hormone Source: RGD
- response to salt stress Source: RGD
- response to xenobiotic stimulus Source: RGD
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Steroidogenesis |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-194002, Glucocorticoid biosynthesis R-RNO-211976, Endogenous sterols |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 11B2, mitochondrialAlternative name(s): Aldosterone synthaseBy similarity Short name: ALDOS Aldosterone-synthesizing enzyme CYPXIB2 Corticosterone 18-monooxygenase, CYP11B2 (EC:1.14.15.5By similarity) Cytochrome P-450Aldo Cytochrome P-450C18 Cytochrome P450-Aldo-1 Steroid 11-beta-hydroxylase, CYP11B2By similarity (EC:1.14.15.4By similarity) Steroid 18-hydroxylase |
Gene namesi | Name:Cyp11b2 Synonyms:Cyp11b-2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2454, Cyp11b2 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: GO_Central
Other locations
- dendrite Source: RGD
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 34 | Mitochondrion1 PublicationAdd BLAST | 34 | |
ChainiPRO_0000003604 | 35 – 510 | Cytochrome P450 11B2, mitochondrialAdd BLAST | 476 |
PTM databases
iPTMneti | P30099 |
PhosphoSitePlusi | P30099 |
Expressioni
Tissue specificityi
Inductioni
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
InParanoidi | P30099 |
PhylomeDBi | P30099 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002399, Cyt_P450_mitochondrial IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00408, MITP450 PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGACDNDFIE LHSRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA
60 70 80 90 100
IPQYSRNKWL KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS
110 120 130 140 150
VMLPEDAEKL HQVESILPRR MHLEPWVAHR ELRGLRRGVF LLNGAEWRFN
160 170 180 190 200
RLKLNPNVLS PKAVQNFVPM VDEVARDFLE ALKKKVRQNA RGSLTMDVQQ
210 220 230 240 250
SLFNYTIEAS NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL
260 270 280 290 300
PRSLTRWTST QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV
310 320 330 340 350
AALITQGALP LDAIKANSME LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL
360 370 380 390 400
RQETLAAEAS IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILNSDL
410 420 430 440 450
VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF
460 470 480 490 500
GFGVRQCLGR RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM AYRFVLMPSS
510
SPVLTFRPIS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 13 | MGACD…IELHS → MNKAPAKAL in AAB60457 (PubMed:8333830).CuratedAdd BLAST | 13 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 84 | E → G1 Publication | 1 | |
Natural varianti | 146 | E → D. | 1 | |
Natural varianti | 261 | Q → R. | 1 | |
Natural varianti | 509 | I → V. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00567 mRNA Translation: BAA00444.1 U14908 mRNA Translation: AAB60457.1 |
PIRi | A35342 JN0615 |
Genome annotation databases
UCSCi | RGD:2454, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00567 mRNA Translation: BAA00444.1 U14908 mRNA Translation: AAB60457.1 |
PIRi | A35342 JN0615 |
3D structure databases
AlphaFoldDBi | P30099 |
SMRi | P30099 |
ModBasei | Search... |
Chemistry databases
BindingDBi | P30099 |
ChEMBLi | CHEMBL3237 |
PTM databases
iPTMneti | P30099 |
PhosphoSitePlusi | P30099 |
Genome annotation databases
UCSCi | RGD:2454, rat |
Organism-specific databases
RGDi | 2454, Cyp11b2 |
Phylogenomic databases
InParanoidi | P30099 |
PhylomeDBi | P30099 |
Enzyme and pathway databases
Reactomei | R-RNO-194002, Glucocorticoid biosynthesis R-RNO-211976, Endogenous sterols |
Miscellaneous databases
PROi | PR:P30099 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002399, Cyt_P450_mitochondrial IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00408, MITP450 PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C11B2_RAT | |
Accessioni | P30099Primary (citable) accession number: P30099 Secondary accession number(s): Q64540 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families