UniProtKB - P30085 (KCY_HUMAN)
Protein
UMP-CMP kinase
Gene
CMPK1
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.UniRule annotation3 Publications
Catalytic activityi
- EC:2.7.4.14UniRule annotation
- EC:2.7.4.14UniRule annotation
- EC:2.7.4.14UniRule annotation
- a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADPUniRule annotationEC:2.7.4.6UniRule annotation
- EC:2.7.4.6UniRule annotation
Cofactori
Mg2+Note: Binds 1 Mg2+ ion per monomer.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | NMPUniRule annotation | 1 | |
Binding sitei | 100 | CMPUniRule annotation | 1 | |
Binding sitei | 134 | ATPUniRule annotation | 1 | |
Binding sitei | 140 | NMPUniRule annotation | 1 | |
Binding sitei | 151 | NMPUniRule annotation | 1 | |
Binding sitei | 179 | ATP; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 13 – 18 | ATPUniRule annotation | 6 | |
Nucleotide bindingi | 61 – 63 | NMPUniRule annotation | 3 | |
Nucleotide bindingi | 93 – 96 | NMPUniRule annotation | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cytidylate kinase activity Source: GO_Central
- nucleoside diphosphate kinase activity Source: UniProtKB
- UMP kinase activity Source: GO_Central
- uridine kinase activity Source: ProtInc
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
- CDP biosynthetic process Source: GO_Central
- nucleobase-containing small molecule interconversion Source: Reactome
- nucleoside diphosphate phosphorylation Source: UniProtKB
- nucleoside triphosphate biosynthetic process Source: UniProtKB
- purine nucleotide metabolic process Source: GO_Central
- pyrimidine ribonucleotide biosynthetic process Source: ProtInc
- UDP biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Pyrimidine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS08663-MONOMER |
BRENDAi | 2.7.4.14, 2681 |
PathwayCommonsi | P30085 |
Reactomei | R-HSA-499943, Interconversion of nucleotide di- and triphosphates |
SABIO-RKi | P30085 |
Names & Taxonomyi
Protein namesi | Recommended name: UMP-CMP kinaseUniRule annotation (EC:2.7.4.14UniRule annotation)Alternative name(s): Deoxycytidylate kinaseUniRule annotation Short name: CKUniRule annotation Short name: dCMP kinaseUniRule annotation Nucleoside-diphosphate kinaseUniRule annotation (EC:2.7.4.6UniRule annotation) Uridine monophosphate/cytidine monophosphate kinaseUniRule annotation Short name: UMP/CMP kinaseUniRule annotation Short name: UMP/CMPKUniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18170, CMPK1 |
MIMi | 191710, gene |
neXtProti | NX_P30085 |
VEuPathDBi | HostDB:ENSG00000162368.13 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 51727 |
OpenTargetsi | ENSG00000162368 |
PharmGKBi | PA162382539 |
Miscellaneous databases
Pharosi | P30085, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5681 |
DrugBanki | DB03403, Cytidine-5'-Monophosphate DB00441, Gemcitabine DB00709, Lamivudine DB08934, Sofosbuvir DB04444, Tetrafluoroaluminate Ion DB03435, Uridine-5'-Diphosphate |
DrugCentrali | P30085 |
Genetic variation databases
BioMutai | CMPK1 |
DMDMi | 12644008 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000158949 | 1 – 196 | UMP-CMP kinaseAdd BLAST | 196 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 33 | PhosphoserineCombined sources | 1 | |
Modified residuei | 43 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 55 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 73 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 106 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 180 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P30085 |
jPOSTi | P30085 |
MassIVEi | P30085 |
MaxQBi | P30085 |
PaxDbi | P30085 |
PeptideAtlasi | P30085 |
PRIDEi | P30085 |
ProteomicsDBi | 20330 54633 [P30085-1] |
TopDownProteomicsi | P30085-1 [P30085-1] |
2D gel databases
OGPi | P30085 |
SWISS-2DPAGEi | P30085 |
PTM databases
iPTMneti | P30085 |
MetOSitei | P30085 |
PhosphoSitePlusi | P30085 |
SwissPalmi | P30085 |
Expressioni
Tissue specificityi
Ubiquitously expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000162368, Expressed in nasal cavity mucosa and 244 other tissues |
ExpressionAtlasi | P30085, baseline and differential |
Genevisiblei | P30085, HS |
Organism-specific databases
HPAi | ENSG00000162368, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
UniRule annotationBinary interactionsi
Hide detailsIsoform 3 [P30085-3]
With | #Exp. | IntAct |
---|---|---|
CAPN1 [P07384] | 3 | EBI-23373346,EBI-1542113 |
CIDEB [Q9UHD4] | 3 | EBI-23373346,EBI-7062247 |
SGTB [Q96EQ0] | 3 | EBI-23373346,EBI-744081 |
Protein-protein interaction databases
BioGRIDi | 119700, 56 interactors |
IntActi | P30085, 14 interactors |
MINTi | P30085 |
STRINGi | 9606.ENSP00000360939 |
Miscellaneous databases
RNActi | P30085, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P30085 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30085 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 63 | NMPbind1 PublicationAdd BLAST | 31 | |
Regioni | 133 – 143 | LID1 PublicationAdd BLAST | 11 |
Domaini
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation1 Publication
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3079, Eukaryota |
GeneTreei | ENSGT00940000160589 |
HOGENOMi | CLU_032354_0_2_1 |
InParanoidi | P30085 |
OMAi | RFQFTHL |
OrthoDBi | 1378291at2759 |
PhylomeDBi | P30085 |
TreeFami | TF354283 |
Family and domain databases
CDDi | cd01428, ADK, 1 hit |
HAMAPi | MF_00235, Adenylate_kinase_Adk, 1 hit MF_03172, Adenylate_kinase_UMP_CMP_kin, 1 hit |
InterProi | View protein in InterPro IPR000850, Adenylat/UMP-CMP_kin IPR033690, Adenylat_kinase_CS IPR027417, P-loop_NTPase IPR006266, UMP_CMP_kinase |
PANTHERi | PTHR23359, PTHR23359, 1 hit |
PRINTSi | PR00094, ADENYLTKNASE |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01359, UMP_CMP_kin_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00113, ADENYLATE_KINASE, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P30085-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKPLVVFVLG GPGAGKGTQC ARIVEKYGYT HLSAGELLRD ERKNPDSQYG
60 70 80 90 100
ELIEKYIKEG KIVPVEITIS LLKREMDQTM AANAQKNKFL IDGFPRNQDN
110 120 130 140 150
LQGWNKTMDG KADVSFVLFF DCNNEICIER CLERGKSSGR SDDNRESLEK
160 170 180 190
RIQTYLQSTK PIIDLYEEMG KVKKIDASKS VDEVFDEVVQ IFDKEG
Isoform 3 (identifier: P30085-3) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MLSRCRSGLLHVLGLSFLLQTRRPILLCSPRLM
Note: May be produced from an in-frame upstream initiation codon. However, experimental evidence indicates that use of the downstream initiation codon is more likely (isoform 1 sequence).Curated
Show »Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5T0D2 | Q5T0D2_HUMAN | Nucleoside-diphosphate kinase | CMPK1 | 169 | Annotation score: | ||
A0A494BXC7 | A0A494BXC7_HUMAN | Nucleoside-diphosphate kinase | CMPK1 | 179 | Annotation score: | ||
E9PJD2 | E9PJD2_HUMAN | UMP-CMP kinase | CMPK1 | 58 | Annotation score: |
Sequence cautioni
The sequence BAG60709 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 27 | Y → I AA sequence (PubMed:8313870).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060085 | 1 | M → MLSRCRSGLLHVLGLSFLLQ TRRPILLCSPRLM in isoform 3. | 1 | |
Alternative sequenceiVSP_046683 | 26 – 74 | Missing in isoform 2. 1 PublicationAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF070416 mRNA Translation: AAF17709.1 AF259961 mRNA Translation: AAG22609.1 AF110643 mRNA Translation: AAD48583.1 AF087865 mRNA Translation: AAP97174.1 AF112216 mRNA Translation: AAF17204.1 AK223014 mRNA Translation: BAD96734.1 AK298502 mRNA Translation: BAG60709.1 Different initiation. AK312693 mRNA Translation: BAG35572.1 AL513322 Genomic DNA No translation available. AL607122 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06869.1 CH471059 Genomic DNA Translation: EAX06871.1 BC014961 mRNA Translation: AAH14961.1 |
CCDSi | CCDS549.1 [P30085-3] |
PIRi | B45482 |
RefSeqi | NP_001129612.1, NM_001136140.1 NP_057392.1, NM_016308.2 [P30085-3] |
Genome annotation databases
Ensembli | ENST00000371873; ENSP00000360939; ENSG00000162368 [P30085-3] |
GeneIDi | 51727 |
KEGGi | hsa:51727 |
UCSCi | uc001cri.3, human [P30085-1] |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF070416 mRNA Translation: AAF17709.1 AF259961 mRNA Translation: AAG22609.1 AF110643 mRNA Translation: AAD48583.1 AF087865 mRNA Translation: AAP97174.1 AF112216 mRNA Translation: AAF17204.1 AK223014 mRNA Translation: BAD96734.1 AK298502 mRNA Translation: BAG60709.1 Different initiation. AK312693 mRNA Translation: BAG35572.1 AL513322 Genomic DNA No translation available. AL607122 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06869.1 CH471059 Genomic DNA Translation: EAX06871.1 BC014961 mRNA Translation: AAH14961.1 |
CCDSi | CCDS549.1 [P30085-3] |
PIRi | B45482 |
RefSeqi | NP_001129612.1, NM_001136140.1 NP_057392.1, NM_016308.2 [P30085-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1TEV | X-ray | 2.10 | A | 1-196 | [»] | |
SMRi | P30085 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119700, 56 interactors |
IntActi | P30085, 14 interactors |
MINTi | P30085 |
STRINGi | 9606.ENSP00000360939 |
Chemistry databases
ChEMBLi | CHEMBL5681 |
DrugBanki | DB03403, Cytidine-5'-Monophosphate DB00441, Gemcitabine DB00709, Lamivudine DB08934, Sofosbuvir DB04444, Tetrafluoroaluminate Ion DB03435, Uridine-5'-Diphosphate |
DrugCentrali | P30085 |
PTM databases
iPTMneti | P30085 |
MetOSitei | P30085 |
PhosphoSitePlusi | P30085 |
SwissPalmi | P30085 |
Genetic variation databases
BioMutai | CMPK1 |
DMDMi | 12644008 |
2D gel databases
OGPi | P30085 |
SWISS-2DPAGEi | P30085 |
Proteomic databases
EPDi | P30085 |
jPOSTi | P30085 |
MassIVEi | P30085 |
MaxQBi | P30085 |
PaxDbi | P30085 |
PeptideAtlasi | P30085 |
PRIDEi | P30085 |
ProteomicsDBi | 20330 54633 [P30085-1] |
TopDownProteomicsi | P30085-1 [P30085-1] |
Protocols and materials databases
Antibodypediai | 32884, 464 antibodies |
DNASUi | 51727 |
Genome annotation databases
Ensembli | ENST00000371873; ENSP00000360939; ENSG00000162368 [P30085-3] |
GeneIDi | 51727 |
KEGGi | hsa:51727 |
UCSCi | uc001cri.3, human [P30085-1] |
Organism-specific databases
CTDi | 51727 |
DisGeNETi | 51727 |
GeneCardsi | CMPK1 |
HGNCi | HGNC:18170, CMPK1 |
HPAi | ENSG00000162368, Low tissue specificity |
MIMi | 191710, gene |
neXtProti | NX_P30085 |
OpenTargetsi | ENSG00000162368 |
PharmGKBi | PA162382539 |
VEuPathDBi | HostDB:ENSG00000162368.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3079, Eukaryota |
GeneTreei | ENSGT00940000160589 |
HOGENOMi | CLU_032354_0_2_1 |
InParanoidi | P30085 |
OMAi | RFQFTHL |
OrthoDBi | 1378291at2759 |
PhylomeDBi | P30085 |
TreeFami | TF354283 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS08663-MONOMER |
BRENDAi | 2.7.4.14, 2681 |
PathwayCommonsi | P30085 |
Reactomei | R-HSA-499943, Interconversion of nucleotide di- and triphosphates |
SABIO-RKi | P30085 |
Miscellaneous databases
BioGRID-ORCSi | 51727, 604 hits in 864 CRISPR screens |
ChiTaRSi | CMPK1, human |
EvolutionaryTracei | P30085 |
GeneWikii | CMPK |
GenomeRNAii | 51727 |
Pharosi | P30085, Tbio |
PROi | PR:P30085 |
RNActi | P30085, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000162368, Expressed in nasal cavity mucosa and 244 other tissues |
ExpressionAtlasi | P30085, baseline and differential |
Genevisiblei | P30085, HS |
Family and domain databases
CDDi | cd01428, ADK, 1 hit |
HAMAPi | MF_00235, Adenylate_kinase_Adk, 1 hit MF_03172, Adenylate_kinase_UMP_CMP_kin, 1 hit |
InterProi | View protein in InterPro IPR000850, Adenylat/UMP-CMP_kin IPR033690, Adenylat_kinase_CS IPR027417, P-loop_NTPase IPR006266, UMP_CMP_kinase |
PANTHERi | PTHR23359, PTHR23359, 1 hit |
PRINTSi | PR00094, ADENYLTKNASE |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01359, UMP_CMP_kin_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00113, ADENYLATE_KINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCY_HUMAN | |
Accessioni | P30085Primary (citable) accession number: P30085 Secondary accession number(s): B2R6S5 Q9UIA2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 209 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families