UniProtKB - P30074 (CHS2_MEDSA)
Protein
Chalcone synthase 2
Gene
CHS2
Organism
Medicago sativa (Alfalfa)
Status
Functioni
The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.4 Publications
Catalytic activityi
- 4-coumaroyl-CoA + 2 H+ + 3 malonyl-CoA = 2',4,4',6'-tetrahydroxychalcone + 3 CO2 + 4 CoAPROSITE-ProRule annotationEC:2.3.1.74PROSITE-ProRule annotation
Kineticsi
- KM=4.1 µM for malonyl-CoA3 Publications
- KM=4.5 µM for 4-coumaroyl-CoA3 Publications
- KM=2.2 µM for benzoyl-CoA3 Publications
- KM=4.1 µM for hexanoyl-CoA3 Publications
- KM=5.1 µM for phenylacetyl-CoA3 Publications
- KM=5.2 µM for feruloyl-CoA3 Publications
: flavonoid biosynthesis Pathwayi
This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 164 | Acyl-thioester intermediateCurated | 1 | |
Binding sitei | 197 | Substrate1 Publication | 1 | |
Binding sitei | 308 | Coenzyme A; via amide nitrogen2 Publications | 1 |
GO - Molecular functioni
- naringenin-chalcone synthase activity Source: UniProtKB
GO - Biological processi
- chalcone biosynthetic process Source: UniProtKB
- flavonoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Flavonoid biosynthesis |
Enzyme and pathway databases
BRENDAi | 2.3.1.74, 3078 |
SABIO-RKi | P30074 |
UniPathwayi | UPA00154 |
Names & Taxonomyi
Protein namesi | Recommended name: Chalcone synthase 2 (EC:2.3.1.74)Alternative name(s): Naringenin-chalcone synthase 2 |
Gene namesi | Name:CHS2 |
Organismi | Medicago sativa (Alfalfa) |
Taxonomic identifieri | 3879 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › NPAAA clade › Hologalegina › IRL clade › Trifolieae › Medicago |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 164 | C → A, D or S: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 215 | F → S, W or Y: Drastically reduces catalytic efficiency. 2 Publications | 1 | |
Mutagenesisi | 256 | G → A: Decreases catalytic efficiency 2-fold. 1 Publication | 1 | |
Mutagenesisi | 256 | G → F or L: Drastically reduces catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 256 | G → V: Decreases catalytic efficiency 7-fold. 1 Publication | 1 | |
Mutagenesisi | 265 | F → V: Decreases catalytic efficiency 2-fold. 1 Publication | 1 | |
Mutagenesisi | 303 | H → A, D, N or T: Drastically reduces catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 303 | H → Q: Decreases catalytic efficiency 13-fold. 1 Publication | 1 | |
Mutagenesisi | 336 | N → A, D, H, K or Q: Drastically reduces catalytic efficiency. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000216007 | 1 – 389 | Chalcone synthase 2Add BLAST | 389 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P30074 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30074 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 55 – 62 | Coenzyme A binding | 8 | |
Regioni | 216 – 217 | Substrate binding | 2 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR012328, Chalcone/stilbene_synth_C IPR018088, Chalcone/stilbene_synthase_AS IPR001099, Chalcone/stilbene_synthase_N IPR011141, Polyketide_synthase_type-III IPR016039, Thiolase-like |
PANTHERi | PTHR11877, PTHR11877, 1 hit |
Pfami | View protein in Pfam PF02797, Chal_sti_synt_C, 1 hit PF00195, Chal_sti_synt_N, 1 hit |
PIRSFi | PIRSF000451, PKS_III, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
PROSITEi | View protein in PROSITE PS00441, CHALCONE_SYNTH, 1 hit |
i Sequence
Sequence statusi: Complete.
P30074-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSVSEIRKA QRAEGPATIL AIGTANPANC VEQSTYPDFY FKITNSEHKT
60 70 80 90 100
ELKEKFQRMC DKSMIKRRYM YLTEEILKEN PNVCEYMAPS LDARQDMVVV
110 120 130 140 150
EVPRLGKEAA VKAIKEWGQP KSKITHLIVC TTSGVDMPGA DYQLTKLLGL
160 170 180 190 200
RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEVTAVTFRG
210 220 230 240 250
PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV WTAQTIAPDS
260 270 280 290 300
EGAIDGHLRE AGLTFHLLKD VPGIVSKNIT KALVEAFEPL GISDYNSIFW
310 320 330 340 350
IAHPGGPAIL DQVEQKLALK PEKMNATREV LSEYGNMSSA CVLFILDEMR
360 370 380
KKSTQNGLKT TGEGLEWGVL FGFGPGLTIE TVVLRSVAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02902 mRNA Translation: AAA02824.1 |
PIRi | S35164 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02902 mRNA Translation: AAA02824.1 |
PIRi | S35164 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BI5 | X-ray | 1.56 | A | 1-389 | [»] | |
1BQ6 | X-ray | 1.56 | A | 2-389 | [»] | |
1CGK | X-ray | 1.84 | A | 1-389 | [»] | |
1CGZ | X-ray | 1.70 | A | 1-389 | [»] | |
1CHW | X-ray | 1.90 | A/B | 1-389 | [»] | |
1CML | X-ray | 1.69 | A | 1-389 | [»] | |
1D6F | X-ray | 1.69 | A | 1-389 | [»] | |
1D6H | X-ray | 2.15 | A | 3-389 | [»] | |
1D6I | X-ray | 2.00 | A/B | 2-389 | [»] | |
1I86 | X-ray | 1.50 | A | 1-389 | [»] | |
1I88 | X-ray | 1.45 | A/B | 1-389 | [»] | |
1I89 | X-ray | 1.86 | A/B | 1-389 | [»] | |
1I8B | X-ray | 1.95 | A/B | 1-389 | [»] | |
1JWX | X-ray | 1.63 | A | 1-389 | [»] | |
1U0V | X-ray | 1.90 | A/B | 1-389 | [»] | |
1U0W | X-ray | 2.00 | A/B/C/D | 1-389 | [»] | |
SMRi | P30074 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00154 |
BRENDAi | 2.3.1.74, 3078 |
SABIO-RKi | P30074 |
Miscellaneous databases
EvolutionaryTracei | P30074 |
Family and domain databases
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR012328, Chalcone/stilbene_synth_C IPR018088, Chalcone/stilbene_synthase_AS IPR001099, Chalcone/stilbene_synthase_N IPR011141, Polyketide_synthase_type-III IPR016039, Thiolase-like |
PANTHERi | PTHR11877, PTHR11877, 1 hit |
Pfami | View protein in Pfam PF02797, Chal_sti_synt_C, 1 hit PF00195, Chal_sti_synt_N, 1 hit |
PIRSFi | PIRSF000451, PKS_III, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
PROSITEi | View protein in PROSITE PS00441, CHALCONE_SYNTH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHS2_MEDSA | |
Accessioni | P30074Primary (citable) accession number: P30074 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | December 11, 2019 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families