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Protein

Chalcone synthase 2

Gene

CHS2

Organism
Medicago sativa (Alfalfa)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.4 Publications

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Kineticsi

  1. KM=4.1 µM for malonyl-CoA3 Publications
  2. KM=4.5 µM for 4-coumaroyl-CoA3 Publications
  3. KM=2.2 µM for benzoyl-CoA3 Publications
  4. KM=4.1 µM for hexanoyl-CoA3 Publications
  5. KM=5.1 µM for phenylacetyl-CoA3 Publications
  6. KM=5.2 µM for feruloyl-CoA3 Publications

    Pathwayi: flavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei164Acyl-thioester intermediateCurated1
    Binding sitei197Substrate1 Publication1
    Binding sitei308Coenzyme A; via amide nitrogen2 Publications1

    GO - Molecular functioni

    • naringenin-chalcone synthase activity Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processFlavonoid biosynthesis

    Enzyme and pathway databases

    BRENDAi2.3.1.74 3078
    SABIO-RKiP30074
    UniPathwayi
    UPA00154

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chalcone synthase 2 (EC:2.3.1.74)
    Alternative name(s):
    Naringenin-chalcone synthase 2
    Gene namesi
    Name:CHS2
    OrganismiMedicago sativa (Alfalfa)
    Taxonomic identifieri3879 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideae50 kb inversion cladeNPAAA cladeHologaleginaIRL cladeTrifolieaeMedicago

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi164C → A, D or S: Loss of activity. 1 Publication1
    Mutagenesisi215F → S, W or Y: Drastically reduces catalytic efficiency. 2 Publications1
    Mutagenesisi256G → A: Decreases catalytic efficiency 2-fold. 1 Publication1
    Mutagenesisi256G → F or L: Drastically reduces catalytic efficiency. 1 Publication1
    Mutagenesisi256G → V: Decreases catalytic efficiency 7-fold. 1 Publication1
    Mutagenesisi265F → V: Decreases catalytic efficiency 2-fold. 1 Publication1
    Mutagenesisi303H → A, D, N or T: Drastically reduces catalytic efficiency. 1 Publication1
    Mutagenesisi303H → Q: Decreases catalytic efficiency 13-fold. 1 Publication1
    Mutagenesisi336N → A, D, H, K or Q: Drastically reduces catalytic efficiency. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002160071 – 389Chalcone synthase 2Add BLAST389

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Structurei

    Secondary structure

    1389
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP30074
    SMRiP30074
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP30074

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni55 – 62Coenzyme A binding8
    Regioni216 – 217Substrate binding2

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.Curated

    Family and domain databases

    Gene3Di3.40.47.10, 2 hits
    InterProiView protein in InterPro
    IPR012328 Chalcone/stilbene_synth_C
    IPR018088 Chalcone/stilbene_synthase_AS
    IPR001099 Chalcone/stilbene_synthase_N
    IPR011141 Polyketide_synthase_type-III
    IPR016039 Thiolase-like
    PANTHERiPTHR11877 PTHR11877, 1 hit
    PfamiView protein in Pfam
    PF02797 Chal_sti_synt_C, 1 hit
    PF00195 Chal_sti_synt_N, 1 hit
    PIRSFiPIRSF000451 PKS_III, 1 hit
    SUPFAMiSSF53901 SSF53901, 2 hits
    PROSITEiView protein in PROSITE
    PS00441 CHALCONE_SYNTH, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P30074-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVSVSEIRKA QRAEGPATIL AIGTANPANC VEQSTYPDFY FKITNSEHKT
    60 70 80 90 100
    ELKEKFQRMC DKSMIKRRYM YLTEEILKEN PNVCEYMAPS LDARQDMVVV
    110 120 130 140 150
    EVPRLGKEAA VKAIKEWGQP KSKITHLIVC TTSGVDMPGA DYQLTKLLGL
    160 170 180 190 200
    RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEVTAVTFRG
    210 220 230 240 250
    PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV WTAQTIAPDS
    260 270 280 290 300
    EGAIDGHLRE AGLTFHLLKD VPGIVSKNIT KALVEAFEPL GISDYNSIFW
    310 320 330 340 350
    IAHPGGPAIL DQVEQKLALK PEKMNATREV LSEYGNMSSA CVLFILDEMR
    360 370 380
    KKSTQNGLKT TGEGLEWGVL FGFGPGLTIE TVVLRSVAI
    Length:389
    Mass (Da):42,706
    Last modified:April 1, 1993 - v1
    Checksum:iE03422EE332A5408
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L02902 mRNA Translation: AAA02824.1
    PIRiS35164

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L02902 mRNA Translation: AAA02824.1
    PIRiS35164

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1BI5X-ray1.56A1-389[»]
    1BQ6X-ray1.56A2-389[»]
    1CGKX-ray1.84A1-389[»]
    1CGZX-ray1.70A1-389[»]
    1CHWX-ray1.90A/B1-389[»]
    1CMLX-ray1.69A1-389[»]
    1D6FX-ray1.69A1-389[»]
    1D6HX-ray2.15A3-389[»]
    1D6IX-ray2.00A/B2-389[»]
    1I86X-ray1.50A1-389[»]
    1I88X-ray1.45A/B1-389[»]
    1I89X-ray1.86A/B1-389[»]
    1I8BX-ray1.95A/B1-389[»]
    1JWXX-ray1.63A1-389[»]
    1U0VX-ray1.90A/B1-389[»]
    1U0WX-ray2.00A/B/C/D1-389[»]
    ProteinModelPortaliP30074
    SMRiP30074
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayi
    UPA00154

    BRENDAi2.3.1.74 3078
    SABIO-RKiP30074

    Miscellaneous databases

    EvolutionaryTraceiP30074

    Family and domain databases

    Gene3Di3.40.47.10, 2 hits
    InterProiView protein in InterPro
    IPR012328 Chalcone/stilbene_synth_C
    IPR018088 Chalcone/stilbene_synthase_AS
    IPR001099 Chalcone/stilbene_synthase_N
    IPR011141 Polyketide_synthase_type-III
    IPR016039 Thiolase-like
    PANTHERiPTHR11877 PTHR11877, 1 hit
    PfamiView protein in Pfam
    PF02797 Chal_sti_synt_C, 1 hit
    PF00195 Chal_sti_synt_N, 1 hit
    PIRSFiPIRSF000451 PKS_III, 1 hit
    SUPFAMiSSF53901 SSF53901, 2 hits
    PROSITEiView protein in PROSITE
    PS00441 CHALCONE_SYNTH, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCHS2_MEDSA
    AccessioniPrimary (citable) accession number: P30074
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: September 12, 2018
    This is version 93 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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