UniProtKB - P30044 (PRDX5_HUMAN)
Protein
Peroxiredoxin-5, mitochondrial
Gene
PRDX5
Organism
Homo sapiens (Human)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.4 Publications
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys Prx, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.2 Publications
Catalytic activityi
- EC:1.11.1.241 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 100 | Cysteine sulfenic acid (-SOH) intermediate2 Publications | 1 |
GO - Molecular functioni
- antioxidant activity Source: UniProtKB
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
- peroxidase activity Source: UniProtKB
- peroxiredoxin activity Source: UniProtKB
- peroxynitrite reductase activity Source: UniProtKB
- RNA polymerase III transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
- thioredoxin peroxidase activity Source: Reactome
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- cellular response to oxidative stress Source: GO_Central
- cellular response to reactive oxygen species Source: UniProtKB
- hydrogen peroxide catabolic process Source: UniProtKB
- inflammatory response Source: UniProtKB
- NADPH oxidation Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of oxidoreductase activity Source: UniProtKB
- negative regulation of transcription by RNA polymerase III Source: UniProtKB
- positive regulation of collagen biosynthetic process Source: UniProtKB
- reactive nitrogen species metabolic process Source: UniProtKB
- regulation of apoptosis involved in tissue homeostasis Source: UniProtKB
- response to oxidative stress Source: UniProtKB
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05016-MONOMER |
BRENDAi | 1.11.1.15, 2681 |
PathwayCommonsi | P30044 |
Reactomei | R-HSA-3299685, Detoxification of Reactive Oxygen Species R-HSA-5628897, TP53 Regulates Metabolic Genes [P30044-2] |
SIGNORi | P30044 |
Protein family/group databases
PeroxiBasei | 4448, HsPrxV |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxiredoxin-5, mitochondrial (EC:1.11.1.241 Publication)Alternative name(s): Alu corepressor 1 Antioxidant enzyme B166 Short name: AOEB166 Liver tissue 2D-page spot 71B PLP Peroxiredoxin V Short name: Prx-V Peroxisomal antioxidant enzyme TPx type VI Thioredoxin peroxidase PMP20 Thioredoxin-dependent peroxiredoxin 5Curated |
Gene namesi | ORF Names:SBBI10 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000126432.13 |
HGNCi | HGNC:9355, PRDX5 |
MIMi | 606583, gene |
neXtProti | NX_P30044 |
Subcellular locationi
Mitochondrion
- Mitochondrion 3 Publications
Peroxisome
- Peroxisome matrix 3 Publications
Other locations
- Cytoplasm 2 Publications
Note: Imported into peroxisomes via peroxisomal targeting signal 1 receptor PEX5.1 Publication
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: UniProtKB
Mitochondrion
- mitochondrial matrix Source: Reactome
- mitochondrion Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Peroxisome
- peroxisomal matrix Source: UniProtKB
- peroxisome Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB
- intracellular membrane-bounded organelle Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, PeroxisomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 100 | C → S: Loss of antioxidant activity. Loss of S-palmitoylation. 2 Publications | 1 | |
Mutagenesisi | 125 | C → S: No change in antioxidant activity. No change in S-palmitoylation levels. 2 Publications | 1 | |
Mutagenesisi | 204 | C → S: Loss of antioxidant activity. No change in S-palmitoylation levels. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 25824 |
OpenTargetsi | ENSG00000126432 |
PharmGKBi | PA33726 |
Miscellaneous databases
Pharosi | P30044, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3627586 |
DrugBanki | DB00995, Auranofin DB03608, Diminazene DB09221, Polaprezinc |
Polymorphism and mutation databases
BioMutai | PRDX5 |
DMDMi | 317373539 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 52 | MitochondrionSequence analysisAdd BLAST | 52 | |
ChainiPRO_0000023793 | 53 – 214 | Peroxiredoxin-5, mitochondrialAdd BLAST | 162 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 75 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 83 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 83 | N6-succinyllysine; alternateBy similarity | 1 | |
Disulfide bondi | 100 ↔ 204 | Redox-activePROSITE-ProRule annotation2 Publications | ||
Lipidationi | 100 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 116 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 171 | PhosphoserineBy similarity | 1 | |
Modified residuei | 182 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
S-palmitoylated (PubMed:31740833). Palmitoylation occurs on the active site, inhibiting its reactivity; therefore PRDX5 palmitoylation status determines its antioxidant capacity (PubMed:31740833).1 Publication
Keywords - PTMi
Acetylation, Disulfide bond, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
CPTACi | CPTAC-572 |
EPDi | P30044 |
jPOSTi | P30044 |
MassIVEi | P30044 |
MaxQBi | P30044 |
PaxDbi | P30044 |
PeptideAtlasi | P30044 |
PRIDEi | P30044 |
ProteomicsDBi | 1092 54624 [P30044-1] 54625 [P30044-2] 797 |
TopDownProteomicsi | P30044-1 [P30044-1] P30044-2 [P30044-2] |
2D gel databases
OGPi | P30044 |
REPRODUCTION-2DPAGEi | IPI00759663 |
SWISS-2DPAGEi | P30044 |
UCD-2DPAGEi | P30044 |
PTM databases
iPTMneti | P30044 |
MetOSitei | P30044 |
PhosphoSitePlusi | P30044 |
SwissPalmi | P30044 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000126432, Expressed in bronchial epithelial cell and 209 other tissues |
Genevisiblei | P30044, HS |
Organism-specific databases
HPAi | ENSG00000126432, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
1 PublicationBinary interactionsi
Hide detailsP30044
With | #Exp. | IntAct |
---|---|---|
CDKN2D [P55273] | 3 | EBI-722161,EBI-745859 |
NUFIP2 [Q7Z417] | 3 | EBI-722161,EBI-1210753 |
SMCP [P49901] | 3 | EBI-722161,EBI-750494 |
SOD1 [P00441] | 10 | EBI-722161,EBI-990792 |
GO - Molecular functioni
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 117352, 134 interactors |
IntActi | P30044, 41 interactors |
MINTi | P30044 |
STRINGi | 9606.ENSP00000265462 |
Miscellaneous databases
RNActi | P30044, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P30044 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P30044 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 214 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 159 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 212 – 214 | Microbody targeting signal1 Publication | 3 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Transit peptidePhylogenomic databases
eggNOGi | KOG0541, Eukaryota |
GeneTreei | ENSGT00390000018173 |
HOGENOMi | CLU_072440_3_1_1 |
InParanoidi | P30044 |
OMAi | GYINHPK |
OrthoDBi | 1281610at2759 |
PhylomeDBi | P30044 |
TreeFami | TF105182 |
Family and domain databases
CDDi | cd03013, PRX5_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR037944, PRX5-like IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10430, PTHR10430, 1 hit |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketIsoform Mitochondrial (identifier: P30044-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGLAGVCALR RSAGYILVGG AGGQSAAAAA RRYSEGEWAS GGVRSFSRAA
60 70 80 90 100
AAMAPIKVGD AIPAVEVFEG EPGNKVNLAE LFKGKKGVLF GVPGAFTPGC
110 120 130 140 150
SKTHLPGFVE QAEALKAKGV QVVACLSVND AFVTGEWGRA HKAEGKVRLL
160 170 180 190 200
ADPTGAFGKE TDLLLDDSLV SIFGNRRLKR FSMVVQDGIV KALNVEPDGT
210
GLTCSLAPNI ISQL
Isoform Cytoplasmic+peroxisomal (identifier: P30044-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-52: Missing.
Sequence cautioni
The sequence AAF17200 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 141 | H → T in AAF04856 (PubMed:10679306).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025049 | 33 | Y → C7 PublicationsCorresponds to variant dbSNP:rs7938623Ensembl. | 1 | |
Natural variantiVAR_036406 | 157 | F → L in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018829 | 1 – 52 | Missing in isoform Cytoplasmic+peroxisomal. 4 PublicationsAdd BLAST | 52 | |
Alternative sequenceiVSP_046682 | 57 – 145 | Missing in isoform 4. 1 PublicationAdd BLAST | 89 | |
Alternative sequenceiVSP_045783 | 102 – 145 | Missing in isoform 3. 1 PublicationAdd BLAST | 44 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF231705 mRNA Translation: AAF78899.1 AF124993 mRNA Translation: AAF27531.1 AF110731 mRNA Translation: AAF03750.1 AF197952 mRNA Translation: AAF04856.1 AJ249483 mRNA Translation: CAB62210.1 AF242525 mRNA Translation: AAF99605.1 AF112212 mRNA Translation: AAF17200.1 Frameshift. CR457203 mRNA Translation: CAG33484.1 DQ247769 Genomic DNA Translation: ABB05181.1 AP001453 Genomic DNA No translation available. AP003774 Genomic DNA No translation available. BC110983 mRNA Translation: AAI10984.1 BC113723 mRNA Translation: AAI13724.1 BC113725 mRNA Translation: AAI13726.1 BC143849 mRNA Translation: AAI43850.1 BC171733 mRNA Translation: AAI71733.1 |
CCDSi | CCDS8069.1 [P30044-1] CCDS8070.1 [P30044-3] CCDS8071.1 [P30044-4] |
RefSeqi | NP_036226.1, NM_012094.4 [P30044-1] NP_857634.1, NM_181651.2 [P30044-3] NP_857635.1, NM_181652.2 [P30044-4] |
Genome annotation databases
Ensembli | ENST00000265462; ENSP00000265462; ENSG00000126432 [P30044-1] ENST00000347941; ENSP00000335363; ENSG00000126432 [P30044-4] ENST00000352435; ENSP00000335334; ENSG00000126432 [P30044-3] |
GeneIDi | 25824 |
KEGGi | hsa:25824 |
UCSCi | uc001nzu.4, human [P30044-1] |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF231705 mRNA Translation: AAF78899.1 AF124993 mRNA Translation: AAF27531.1 AF110731 mRNA Translation: AAF03750.1 AF197952 mRNA Translation: AAF04856.1 AJ249483 mRNA Translation: CAB62210.1 AF242525 mRNA Translation: AAF99605.1 AF112212 mRNA Translation: AAF17200.1 Frameshift. CR457203 mRNA Translation: CAG33484.1 DQ247769 Genomic DNA Translation: ABB05181.1 AP001453 Genomic DNA No translation available. AP003774 Genomic DNA No translation available. BC110983 mRNA Translation: AAI10984.1 BC113723 mRNA Translation: AAI13724.1 BC113725 mRNA Translation: AAI13726.1 BC143849 mRNA Translation: AAI43850.1 BC171733 mRNA Translation: AAI71733.1 |
CCDSi | CCDS8069.1 [P30044-1] CCDS8070.1 [P30044-3] CCDS8071.1 [P30044-4] |
RefSeqi | NP_036226.1, NM_012094.4 [P30044-1] NP_857634.1, NM_181651.2 [P30044-3] NP_857635.1, NM_181652.2 [P30044-4] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H4O | X-ray | 1.95 | A/B/C/D/E/F/G/H | 54-214 | [»] | |
1HD2 | X-ray | 1.50 | A | 54-214 | [»] | |
1OC3 | X-ray | 2.00 | A/B/C | 54-214 | [»] | |
1URM | X-ray | 1.70 | A | 54-214 | [»] | |
2VL2 | X-ray | 1.92 | A/B/C | 54-214 | [»] | |
2VL3 | X-ray | 1.83 | A/B/C | 54-214 | [»] | |
2VL9 | X-ray | 2.70 | A/B/C/D | 54-214 | [»] | |
3MNG | X-ray | 1.45 | A | 54-214 | [»] | |
4K7I | X-ray | 2.25 | A/B/C | 54-214 | [»] | |
4K7N | X-ray | 2.30 | A/B/C | 54-214 | [»] | |
4K7O | X-ray | 1.98 | A/B/C | 54-214 | [»] | |
4MMM | X-ray | 1.47 | A/C/E/G | 54-214 | [»] | |
SMRi | P30044 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117352, 134 interactors |
IntActi | P30044, 41 interactors |
MINTi | P30044 |
STRINGi | 9606.ENSP00000265462 |
Chemistry databases
ChEMBLi | CHEMBL3627586 |
DrugBanki | DB00995, Auranofin DB03608, Diminazene DB09221, Polaprezinc |
Protein family/group databases
PeroxiBasei | 4448, HsPrxV |
PTM databases
iPTMneti | P30044 |
MetOSitei | P30044 |
PhosphoSitePlusi | P30044 |
SwissPalmi | P30044 |
Polymorphism and mutation databases
BioMutai | PRDX5 |
DMDMi | 317373539 |
2D gel databases
OGPi | P30044 |
REPRODUCTION-2DPAGEi | IPI00759663 |
SWISS-2DPAGEi | P30044 |
UCD-2DPAGEi | P30044 |
Proteomic databases
CPTACi | CPTAC-572 |
EPDi | P30044 |
jPOSTi | P30044 |
MassIVEi | P30044 |
MaxQBi | P30044 |
PaxDbi | P30044 |
PeptideAtlasi | P30044 |
PRIDEi | P30044 |
ProteomicsDBi | 1092 54624 [P30044-1] 54625 [P30044-2] 797 |
TopDownProteomicsi | P30044-1 [P30044-1] P30044-2 [P30044-2] |
Protocols and materials databases
Antibodypediai | 3276, 423 antibodies |
DNASUi | 25824 |
Genome annotation databases
Ensembli | ENST00000265462; ENSP00000265462; ENSG00000126432 [P30044-1] ENST00000347941; ENSP00000335363; ENSG00000126432 [P30044-4] ENST00000352435; ENSP00000335334; ENSG00000126432 [P30044-3] |
GeneIDi | 25824 |
KEGGi | hsa:25824 |
UCSCi | uc001nzu.4, human [P30044-1] |
Organism-specific databases
CTDi | 25824 |
DisGeNETi | 25824 |
EuPathDBi | HostDB:ENSG00000126432.13 |
GeneCardsi | PRDX5 |
HGNCi | HGNC:9355, PRDX5 |
HPAi | ENSG00000126432, Low tissue specificity |
MIMi | 606583, gene |
neXtProti | NX_P30044 |
OpenTargetsi | ENSG00000126432 |
PharmGKBi | PA33726 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0541, Eukaryota |
GeneTreei | ENSGT00390000018173 |
HOGENOMi | CLU_072440_3_1_1 |
InParanoidi | P30044 |
OMAi | GYINHPK |
OrthoDBi | 1281610at2759 |
PhylomeDBi | P30044 |
TreeFami | TF105182 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05016-MONOMER |
BRENDAi | 1.11.1.15, 2681 |
PathwayCommonsi | P30044 |
Reactomei | R-HSA-3299685, Detoxification of Reactive Oxygen Species R-HSA-5628897, TP53 Regulates Metabolic Genes [P30044-2] |
SIGNORi | P30044 |
Miscellaneous databases
BioGRID-ORCSi | 25824, 9 hits in 850 CRISPR screens |
ChiTaRSi | PRDX5, human |
EvolutionaryTracei | P30044 |
GeneWikii | PRDX5 |
GenomeRNAii | 25824 |
Pharosi | P30044, Tbio |
PROi | PR:P30044 |
RNActi | P30044, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000126432, Expressed in bronchial epithelial cell and 209 other tissues |
Genevisiblei | P30044, HS |
Family and domain databases
CDDi | cd03013, PRX5_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR037944, PRX5-like IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10430, PTHR10430, 1 hit |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRDX5_HUMAN | |
Accessioni | P30044Primary (citable) accession number: P30044 Secondary accession number(s): A6NC19 Q9UKX4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | January 11, 2011 | |
Last modified: | December 2, 2020 | |
This is version 214 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations