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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

nadC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119SubstrateBy similarity1
Binding sitei176SubstrateBy similarity1
Binding sitei186SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei236SubstrateBy similarity1

GO - Molecular functioni

  • nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: EcoCyc

GO - Biological processi

  • 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
  • NAD biosynthetic process Source: EcoliWiki
  • quinolinate catabolic process Source: GO_Central

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:QUINOPRIBOTRANS-MONOMER
MetaCyc:QUINOPRIBOTRANS-MONOMER
BRENDAi2.4.2.19 2026
UniPathwayiUPA00253; UER00331

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:nadC
Ordered Locus Names:b0109, JW0105
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11546 nadC

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001559422 – 297Nicotinate-nucleotide pyrophosphorylase [carboxylating]Add BLAST296

Proteomic databases

EPDiP30011
PaxDbiP30011
PRIDEiP30011

Interactioni

Subunit structurei

Homodimer Hexamer formed by 3 homodimers (By similarity). Homodimer.By similarity

Protein-protein interaction databases

BioGridi4263544, 20 interactors
DIPiDIP-10293N
IntActiP30011, 4 interactors
STRINGi316385.ECDH10B_0089

Structurei

3D structure databases

ProteinModelPortaliP30011
SMRiP30011
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 154Substrate bindingBy similarity3
Regioni259 – 261Substrate bindingBy similarity3
Regioni280 – 282Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

eggNOGiENOG4105D18 Bacteria
COG0157 LUCA
HOGENOMiHOG000224022
InParanoidiP30011
KOiK00767
OMAiVIMLDNM
PhylomeDBiP30011

Family and domain databases

CDDicd01572 QPRTase, 1 hit
Gene3Di3.20.20.70, 1 hit
3.90.1170.20, 2 hits
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR004393 NadC
IPR027277 NadC/ModD
IPR036068 Nicotinate_pribotase_like_C
IPR037128 Quinolinate_PRibosylTase_N_sf
IPR002638 Quinolinate_PRibosylTrfase_C
IPR022412 Quinolinate_PRibosylTrfase_N
PANTHERiPTHR32179 PTHR32179, 1 hit
PfamiView protein in Pfam
PF01729 QRPTase_C, 1 hit
PF02749 QRPTase_N, 1 hit
PIRSFiPIRSF006250 NadC_ModD, 1 hit
SUPFAMiSSF51690 SSF51690, 1 hit
TIGRFAMsiTIGR00078 nadC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRRYNPDT RRDELLERIN LDIPGAVAQA LREDLGGTVD ANNDITAKLL
60 70 80 90 100
PENSRSHATV ITRENGVFCG KRWVEEVFIQ LAGDDVTIIW HVDDGDVINA
110 120 130 140 150
NQSLFELEGP SRVLLTGERT ALNFVQTLSG VASKVRHYVE LLEGTNTQLL
160 170 180 190 200
DTRKTLPGLR SALKYAVLCG GGANHRLGLS DAFLIKENHI IASGSVRQAV
210 220 230 240 250
EKASWLHPDA PVEVEVENLE ELDEALKAGA DIIMLDNFET EQMREAVKRT
260 270 280 290
NGKALLEVSG NVTDKTLREF AETGVDFISV GALTKHVQAL DLSMRFR
Length:297
Mass (Da):32,762
Last modified:January 23, 2007 - v7
Checksum:i0B5DA69D9B67EE39
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119R → P in AAB00467 (PubMed:8561507).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28105 Genomic DNA Translation: AAC36922.1
L20833 Genomic DNA Translation: AAB00467.1
U00096 Genomic DNA Translation: AAC73220.1
AP009048 Genomic DNA Translation: BAE76041.1
X15237 Genomic DNA No translation available.
PIRiE64733
RefSeqiNP_414651.1, NC_000913.3
WP_001135174.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73220; AAC73220; b0109
BAE76041; BAE76041; BAE76041
GeneIDi948869
KEGGiecj:JW0105
eco:b0109
PATRICifig|1411691.4.peg.2173

Entry informationi

Entry nameiNADC_ECOLI
AccessioniPrimary (citable) accession number: P30011
Secondary accession number(s): Q2MCG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 154 of the entry and version 7 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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