UniProtKB - P29975 (AQP1_RAT)
Protein
Aquaporin-1
Gene
Aqp1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Miscellaneous
Pharmacologically inhibited by submillimolar concentrations of mercury.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 56 | Substrate discriminationBy similarity | 1 | |
Sitei | 180 | Substrate discriminationBy similarity | 1 | |
Sitei | 189 | Hg(2+)-sensitive residue | 1 | |
Sitei | 195 | Substrate discriminationBy similarity | 1 |
GO - Molecular functioni
- ammonium transmembrane transporter activity Source: UniProtKB
- carbon dioxide transmembrane transporter activity Source: UniProtKB
- ephrin receptor binding Source: RGD
- glycerol transmembrane transporter activity Source: UniProtKB
- identical protein binding Source: RGD
- intracellular cGMP-activated cation channel activity Source: UniProtKB
- nitric oxide transmembrane transporter activity Source: UniProtKB
- potassium channel activity Source: UniProtKB
- potassium ion transmembrane transporter activity Source: RGD
- transmembrane transporter activity Source: RGD
- water channel activity Source: RGD
- water transmembrane transporter activity Source: RGD
GO - Biological processi
- ammonium transport Source: UniProtKB
- camera-type eye morphogenesis Source: RGD
- carbon dioxide transmembrane transport Source: UniProtKB
- carbon dioxide transport Source: UniProtKB
- cellular homeostasis Source: UniProtKB
- cellular hyperosmotic response Source: UniProtKB
- cellular response to cAMP Source: UniProtKB
- cellular response to copper ion Source: UniProtKB
- cellular response to dexamethasone stimulus Source: UniProtKB
- cellular response to hydrogen peroxide Source: UniProtKB
- cellular response to hypoxia Source: UniProtKB
- cellular response to inorganic substance Source: UniProtKB
- cellular response to mechanical stimulus Source: UniProtKB
- cellular response to mercury ion Source: UniProtKB
- cellular response to nitric oxide Source: RGD
- cellular response to retinoic acid Source: UniProtKB
- cellular response to salt stress Source: UniProtKB
- cellular response to UV Source: UniProtKB
- cellular water homeostasis Source: UniProtKB
- cell volume homeostasis Source: UniProtKB
- cerebrospinal fluid secretion Source: RGD
- cGMP-mediated signaling Source: UniProtKB
- corticotropin secretion Source: RGD
- establishment or maintenance of actin cytoskeleton polarity Source: UniProtKB
- glomerular filtration Source: RGD
- glycerol transport Source: UniProtKB
- hyperosmotic response Source: RGD
- hyperosmotic salinity response Source: RGD
- lateral ventricle development Source: UniProtKB
- lipid digestion Source: RGD
- maintenance of symbiont-containing vacuole by host Source: UniProtKB
- metanephric descending thin limb development Source: RGD
- metanephric glomerulus vasculature development Source: RGD
- metanephric proximal convoluted tubule segment 2 development Source: RGD
- metanephric proximal straight tubule development Source: RGD
- multicellular organismal water homeostasis Source: RGD
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
- nitric oxide transport Source: UniProtKB
- odontogenesis Source: RGD
- pancreatic juice secretion Source: RGD
- positive regulation of angiogenesis Source: UniProtKB
- positive regulation of cell migration Source: RGD
- positive regulation of epithelial cell migration Source: RGD
- positive regulation of fibroblast proliferation Source: UniProtKB
- positive regulation of lamellipodium assembly Source: RGD
- positive regulation of saliva secretion Source: UniProtKB
- potassium ion transport Source: RGD
- renal water absorption Source: RGD
- renal water transport Source: UniProtKB
- response to drug Source: RGD
- response to estrogen Source: RGD
- response to hormone Source: RGD
- secretion by cell Source: RGD
- secretory granule organization Source: RGD
- sensory perception of pain Source: RGD
- transepithelial water transport Source: UniProtKB
- water homeostasis Source: RGD
- water transport Source: RGD
- wound healing Source: RGD
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
Reactomei | R-RNO-1237044, Erythrocytes take up carbon dioxide and release oxygen R-RNO-1247673, Erythrocytes take up oxygen and release carbon dioxide R-RNO-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-RNO-432047, Passive transport by Aquaporins |
Names & Taxonomyi
Protein namesi | Recommended name: Aquaporin-1Short name: AQP-1 Alternative name(s): Aquaporin-CHIP Water channel protein for red blood cells and kidney proximal tubule |
Gene namesi | Name:Aqp1 Synonyms:Chip28 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2141, Aqp1 |
Subcellular locationi
Plasma membrane
Extracellular region or secreted
- extracellular exosome Source: RGD
Nucleus
- nuclear membrane Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- basal plasma membrane Source: UniProtKB
- basolateral plasma membrane Source: UniProtKB
- brush border membrane Source: UniProtKB
- caveola Source: RGD
- neuronal cell body membrane Source: RGD
- plasma membrane Source: UniProtKB
- sarcolemma Source: UniProtKB
Other locations
- apical part of cell Source: RGD
- axon Source: RGD
- axon terminus Source: RGD
- brush border Source: UniProtKB
- cytoplasm Source: UniProtKB
- integral component of membrane Source: RGD
- membrane Source: RGD
- neuron projection Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicBy similarity | 7 | |
Transmembranei | 8 – 36 | Helical; Name=Helix 1By similarityAdd BLAST | 29 | |
Topological domaini | 37 – 48 | ExtracellularBy similarityAdd BLAST | 12 | |
Transmembranei | 49 – 66 | Helical; Name=Helix 2By similarityAdd BLAST | 18 | |
Topological domaini | 67 – 70 | CytoplasmicBy similarity | 4 | |
Intramembranei | 71 – 76 | By similarity | 6 | |
Intramembranei | 77 – 84 | Helical; Name=Helix BBy similarity | 8 | |
Topological domaini | 85 – 94 | CytoplasmicBy similarity | 10 | |
Transmembranei | 95 – 115 | Helical; Name=Helix 3By similarityAdd BLAST | 21 | |
Topological domaini | 116 – 136 | ExtracellularBy similarityAdd BLAST | 21 | |
Transmembranei | 137 – 155 | Helical; Name=Helix 4By similarityAdd BLAST | 19 | |
Topological domaini | 156 – 166 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 167 – 183 | Helical; Name=Helix 5By similarityAdd BLAST | 17 | |
Topological domaini | 184 – 186 | ExtracellularBy similarity | 3 | |
Intramembranei | 187 – 192 | By similarity | 6 | |
Intramembranei | 193 – 200 | Helical; Name=Helix EBy similarity | 8 | |
Topological domaini | 201 – 207 | ExtracellularBy similarity | 7 | |
Transmembranei | 208 – 228 | Helical; Name=Helix 6By similarityAdd BLAST | 21 | |
Topological domaini | 229 – 269 | CytoplasmicBy similarityAdd BLAST | 41 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063924 | 1 – 269 | Aquaporin-1Add BLAST | 269 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 42 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 205 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 247 | PhosphoserineBy similarity | 1 | |
Modified residuei | 253 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 262 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P29975 |
PRIDEi | P29975 |
PTM databases
GlyGeni | P29975, 2 sites |
iPTMneti | P29975 |
PhosphoSitePlusi | P29975 |
SwissPalmi | P29975 |
Expressioni
Tissue specificityi
Erythrocytes and renal tubules.
Gene expression databases
Bgeei | ENSRNOG00000011648, Expressed in adult mammalian kidney and 20 other tissues |
ExpressionAtlasi | P29975, baseline and differential |
Genevisiblei | P29975, RN |
Interactioni
Subunit structurei
Homotetramer (By similarity).
Interacts with EPHB2; involved in endolymph production in the inner ear.
Identified in a complex with STOM (By similarity).
By similarityGO - Molecular functioni
- ephrin receptor binding Source: RGD
- identical protein binding Source: RGD
Protein-protein interaction databases
CORUMi | P29975 |
STRINGi | 10116.ENSRNOP00000015692 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 76 – 78 | NPA 1 | 3 | |
Motifi | 192 – 194 | NPA 2 | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 159 – 162 | Poly-Arg | 4 |
Domaini
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000157015 |
InParanoidi | P29975 |
OMAi | ELFGTFW |
OrthoDBi | 1152704at2759 |
PhylomeDBi | P29975 |
TreeFami | TF312940 |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR023274, Aquaporin_1 IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR02013, AQUAPORIN1 PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P29975-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASEIKKKLF WRAVVAEFLA MTLFVFISIG SALGFNYPLE RNQTLVQDNV
60 70 80 90 100
KVSLAFGLSI ATLAQSVGHI SGAHLNPAVT LGLLLSCQIS ILRAVMYIIA
110 120 130 140 150
QCVGAIVASA ILSGITSSLL ENSLGRNDLA RGVNSGQGLG IEIIGTLQLV
160 170 180 190 200
LCVLATTDRR RRDLGGSAPL AIGLSVALGH LLAIDYTGCG INPARSFGSA
210 220 230 240 250
VLTRNFSNHW IFWVGPFIGS ALAVLIYDFI LAPRSSDFTD RMKVWTSGQV
260
EEYDLDADDI NSRVEMKPK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K3E0 | A0A0G2K3E0_RAT | Aquaporin-1 | Aqp1 | 247 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | S → T in CAA50395 (PubMed:8508530).Curated | 1 | |
Sequence conflicti | 5 | I → F in CAA49761 (PubMed:8421053).Curated | 1 | |
Sequence conflicti | 75 | L → S in AAB46624 (PubMed:7530838).Curated | 1 | |
Sequence conflicti | 159 | R → Q in CAA50395 (PubMed:8508530).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67948 mRNA Translation: CAA48134.1 L07268 mRNA Translation: AAB46624.1 X70257 mRNA Translation: CAA49761.1 X71069 mRNA Translation: CAA50395.1 BC090068 mRNA Translation: AAH90068.1 |
PIRi | JC1320 |
RefSeqi | NP_036910.1, NM_012778.1 |
Genome annotation databases
Ensembli | ENSRNOT00000015692; ENSRNOP00000015692; ENSRNOG00000011648 |
GeneIDi | 25240 |
KEGGi | rno:25240 |
UCSCi | RGD:2141, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67948 mRNA Translation: CAA48134.1 L07268 mRNA Translation: AAB46624.1 X70257 mRNA Translation: CAA49761.1 X71069 mRNA Translation: CAA50395.1 BC090068 mRNA Translation: AAH90068.1 |
PIRi | JC1320 |
RefSeqi | NP_036910.1, NM_012778.1 |
3D structure databases
SMRi | P29975 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | P29975 |
STRINGi | 10116.ENSRNOP00000015692 |
PTM databases
GlyGeni | P29975, 2 sites |
iPTMneti | P29975 |
PhosphoSitePlusi | P29975 |
SwissPalmi | P29975 |
Proteomic databases
PaxDbi | P29975 |
PRIDEi | P29975 |
Genome annotation databases
Ensembli | ENSRNOT00000015692; ENSRNOP00000015692; ENSRNOG00000011648 |
GeneIDi | 25240 |
KEGGi | rno:25240 |
UCSCi | RGD:2141, rat |
Organism-specific databases
CTDi | 358 |
RGDi | 2141, Aqp1 |
Phylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000157015 |
InParanoidi | P29975 |
OMAi | ELFGTFW |
OrthoDBi | 1152704at2759 |
PhylomeDBi | P29975 |
TreeFami | TF312940 |
Enzyme and pathway databases
Reactomei | R-RNO-1237044, Erythrocytes take up carbon dioxide and release oxygen R-RNO-1247673, Erythrocytes take up oxygen and release carbon dioxide R-RNO-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-RNO-432047, Passive transport by Aquaporins |
Miscellaneous databases
PROi | PR:P29975 |
Gene expression databases
Bgeei | ENSRNOG00000011648, Expressed in adult mammalian kidney and 20 other tissues |
ExpressionAtlasi | P29975, baseline and differential |
Genevisiblei | P29975, RN |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR023274, Aquaporin_1 IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR02013, AQUAPORIN1 PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AQP1_RAT | |
Accessioni | P29975Primary (citable) accession number: P29975 Secondary accession number(s): Q5EB50, Q7TN36 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 183 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families