UniProtKB - P29853 (BGAL_ASPNG)
Protein
Beta-galactosidase
Gene
lacA
Organism
Aspergillus niger
Status
Functioni
Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.
Catalytic activityi
- Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. EC:3.2.1.23
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 200 | Proton donorSequence analysis | 1 | |
Active sitei | 298 | NucleophileSequence analysis | 1 |
GO - Molecular functioni
- beta-galactosidase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16612 |
BRENDAi | 3.2.1.23, 518 |
Protein family/group databases
CAZyi | GH35, Glycoside Hydrolase Family 35 |
CLAEi | LAC35A_ASPNG |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-galactosidase (EC:3.2.1.23)Alternative name(s): Lactase-N Short name: Lactase INN: Tilactase |
Gene namesi | Name:lacA |
Organismi | Aspergillus niger |
Taxonomic identifieri | 5061 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Circumdati |
Pathology & Biotechi
Pharmaceutical usei
Capable of effecting hydrolysis of lactose in situ in the gastrointestinal tract of lactase-deficient subjects when given as replacement therapy at mealtime.
Chemistry databases
ChEMBLi | CHEMBL4753 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000012192 | 20 – 1006 | Beta-galactosidaseAdd BLAST | 987 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 156 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 373 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 402 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 422 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 478 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 522 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 622 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 739 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 760 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 777 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 805 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinInteractioni
Protein-protein interaction databases
STRINGi | 5061.CADANGAP00001173 |
Chemistry databases
BindingDBi | P29853 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 35 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG0496, Eukaryota |
Family and domain databases
Gene3Di | 2.102.20.10, 1 hit 2.60.120.260, 1 hit 2.60.390.10, 1 hit |
InterProi | View protein in InterPro IPR018954, Betagal_dom2 IPR037110, Betagal_dom2_sf IPR025972, BetaGal_dom3 IPR036833, BetaGal_dom3_sf IPR025300, BetaGal_jelly_roll_dom IPR008979, Galactose-bd-like_sf IPR031330, Gly_Hdrlase_35_cat IPR019801, Glyco_hydro_35_CS IPR001944, Glycoside_Hdrlase_35 IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR23421, PTHR23421, 2 hits |
Pfami | View protein in Pfam PF10435, BetaGal_dom2, 1 hit PF13363, BetaGal_dom3, 1 hit PF13364, BetaGal_dom4_5, 2 hits PF01301, Glyco_hydro_35, 1 hit |
PRINTSi | PR00742, GLHYDRLASE35 |
SMARTi | View protein in SMART SM01029, BetaGal_dom2, 1 hit |
SUPFAMi | SSF117100, SSF117100, 1 hit SSF49785, SSF49785, 2 hits SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS01182, GLYCOSYL_HYDROL_F35, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P29853-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLSSACAIA LLAAQAAGAS IKHRINGFTL TEHSDPAKRE LLQKYVTWDD
60 70 80 90 100
KSLFINGERI MIFSGEFHPF RLPVKELQLD IFQKVKALGF NCVSFYVDWA
110 120 130 140 150
LVEGKPGEYR ADGIFDLEPF FDAASEAGIY LLARPGPYIN AESSGGGFPG
160 170 180 190 200
WLQRVNGTLR SSDKAYLDAT DNYVSHVAAT IAKYQITNGG PIILYQPENE
210 220 230 240 250
YTSGCSGVEF PDPVYMQYVE DQARNAGVVI PLINNDASAS GNNAPGTGKG
260 270 280 290 300
AVDIYGHDSY PLGFDCANPT VWPSGDLPTN FRTLHLEQSP TTPYAIVEFQ
310 320 330 340 350
GGSYDPWGGP GFAACSELLN NEFERVFYKN DFSFQIAIMN LYMIFGGTNW
360 370 380 390 400
GNLGYPNGYT SYDYGSAVTE SRNITREKYS ELKLLGNFAK VSPGYLTASP
410 420 430 440 450
GNLTTSGYAD TTDLTVTPLL GNSTGSFFVV RHSDYSSEES TSYKLRLPTS
460 470 480 490 500
AGSVTIPQLG GTLTLNGRDS KIHVTDHNVS GTNIIYSTAE VFTWKKFADG
510 520 530 540 550
KVLVLYGGAG EHHELAISTK SNVTVIEGSE SGISSKQTSS SVVVGWDVST
560 570 580 590 600
TRRIIQVGDL KILLLDRNSA YNYWVPQLAT DGTSPGFSTP EKVASSIIVK
610 620 630 640 650
AGYLVRTAYL KGSGLYLTAD FNATTSVEVI GVPSTAKNLF INGDKTSHTV
660 670 680 690 700
DKNGIWSATV DYNAPDISLP SLKDLDWKYV DTLPEIQSSY DDSLWPAADL
710 720 730 740 750
KQTKNTLRSL TTPTSLYSSD YGFHTGYLLY RGHFTATGNE STFAIDTQGG
760 770 780 790 800
SAFGSSVWLN GTYLGSWTGL YANSDYNATY NLPQLQAGKT YVITVVIDNM
810 820 830 840 850
GLEENWTVGE DLMKSPRGIS TSCLPDGQAA PISWKLTGNL GGEDYEDKVR
860 870 880 890 900
GPLNEGGLYA ERQGFHQPEP PSQNWKSSSP LEGLSEAGIG FYSASFDLDL
910 920 930 940 950
PKDGMSHCSS TSVTALRHPR TACRSTSTDI VCEIHKQHRT SDQLPCPRGN
960 970 980 990 1000
PELSRNELVG GDPVALDSAG GKLESLELSY TTPVLTALGE VESVDQPKYK
KRKGAY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 206 | S → C in CAA00105 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06037 Genomic DNA Translation: AAA32696.1 S37150 mRNA Translation: AAC60538.1 A00968 Unassigned DNA Translation: CAA00105.1 |
PIRi | T31685 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06037 Genomic DNA Translation: AAA32696.1 S37150 mRNA Translation: AAC60538.1 A00968 Unassigned DNA Translation: CAA00105.1 |
PIRi | T31685 |
3D structure databases
SMRi | P29853 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 5061.CADANGAP00001173 |
Chemistry databases
BindingDBi | P29853 |
ChEMBLi | CHEMBL4753 |
Protein family/group databases
CAZyi | GH35, Glycoside Hydrolase Family 35 |
CLAEi | LAC35A_ASPNG |
Phylogenomic databases
eggNOGi | KOG0496, Eukaryota |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16612 |
BRENDAi | 3.2.1.23, 518 |
Family and domain databases
Gene3Di | 2.102.20.10, 1 hit 2.60.120.260, 1 hit 2.60.390.10, 1 hit |
InterProi | View protein in InterPro IPR018954, Betagal_dom2 IPR037110, Betagal_dom2_sf IPR025972, BetaGal_dom3 IPR036833, BetaGal_dom3_sf IPR025300, BetaGal_jelly_roll_dom IPR008979, Galactose-bd-like_sf IPR031330, Gly_Hdrlase_35_cat IPR019801, Glyco_hydro_35_CS IPR001944, Glycoside_Hdrlase_35 IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR23421, PTHR23421, 2 hits |
Pfami | View protein in Pfam PF10435, BetaGal_dom2, 1 hit PF13363, BetaGal_dom3, 1 hit PF13364, BetaGal_dom4_5, 2 hits PF01301, Glyco_hydro_35, 1 hit |
PRINTSi | PR00742, GLHYDRLASE35 |
SMARTi | View protein in SMART SM01029, BetaGal_dom2, 1 hit |
SUPFAMi | SSF117100, SSF117100, 1 hit SSF49785, SSF49785, 2 hits SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS01182, GLYCOSYL_HYDROL_F35, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BGAL_ASPNG | |
Accessioni | P29853Primary (citable) accession number: P29853 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 108 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
PharmaceuticalDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families