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Protein

Alpha-amylase

Gene

tam

Organism
Thermomonospora curvata
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Temperature dependencei

Optimum temperature is 65 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi130CalciumBy similarity1
Metal bindingi189CalciumBy similarity1
Active sitei219NucleophileBy similarity1
Metal bindingi223Calcium; via carbonyl oxygenBy similarity1
Active sitei253Proton donorBy similarity1
Sitei313Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:tam
OrganismiThermomonospora curvata
Taxonomic identifieri2020 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesThermomonosporaceaeThermomonospora

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000000134634 – 605Alpha-amylaseAdd BLAST572

Expressioni

Inductioni

By maltose or maltodextrins, even in the presence of glucose.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP29750
SMRiP29750
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini500 – 605CBM20PROSITE-ProRule annotationAdd BLAST106

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF00686 CBM_20, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29750-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGVRRSLAAL LAALLGCATS LVALTVAASP AHAAPSGNRD VIVHLFQWRW
60 70 80 90 100
KSIADECRTT LGPHGFGAVQ VSPPQEHVVL PAEDYPWWQD YQPVSYKLDQ
110 120 130 140 150
TRRGSRADFI DMVNTCREAG VKIYVDAVIN HMTGTGSAGA GPGSAGSSYS
160 170 180 190 200
KYDYPGIYQS QDFNDCRRDI TNWNDKWEVQ HCELVGLADL KTSSPYVQDR
210 220 230 240 250
IAAYLNELID LGVAGFRIDA AKHIPEGDLQ AILSRLKNVH PAWGGGKPYI
260 270 280 290 300
FQEVIADSTI STGSYTHLGS VTEFQYHRDI SHAFANGNIA HLTGLGSGLT
310 320 330 340 350
PSDKAVVFVV NHDTQRYEPI LTHTDRARYD LAQKFMLAHP YGTPKVMSSY
360 370 380 390 400
TWSGDDKAGP PMHSDGTTRP TDCSADRWLC EHRAVAGMVG FHNAVAGQGI
410 420 430 440 450
GSAVTDGNGR LAFARGSAGY AAFNATNTAW TRTFTTSLPD GVYCDVANGT
460 470 480 490 500
FVDGVCDGPS YQVSGGKFTA TVPANGAVAL HVEAPGSCGP DGCGTPPGGG
510 520 530 540 550
DDCTTVTARF HATVTTWYGQ EVAVVGSIPE LGSWQPAQGV RLRTDSGTYP
560 570 580 590 600
VWSGAVDLPA GVGFEYKYVK LNRTAPWSGS RAATASPPWM TSGGGCSQNF

YDSWR
Length:605
Mass (Da):64,735
Last modified:April 1, 1993 - v1
Checksum:i80ABB8F08A69B69A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59159 Genomic DNA Translation: CAA41881.1
PIRiJH0638

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59159 Genomic DNA Translation: CAA41881.1
PIRiJH0638

3D structure databases

ProteinModelPortaliP29750
SMRiP29750
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF00686 CBM_20, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY_THECU
AccessioniPrimary (citable) accession number: P29750
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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