UniProtKB - P29728 (OAS2_HUMAN)
Protein
2'-5'-oligoadenylate synthase 2
Gene
OAS2
Organism
Homo sapiens (Human)
Status
Functioni
Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response (PubMed:10464285, PubMed:9880569). Activated by detection of double stranded RNA (dsRNA): polymerizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNASEL) leading to its dimerization and subsequent activation (PubMed:10464285, PubMed:9880569, PubMed:11682059). Activation of RNASEL leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication (PubMed:10464285, PubMed:9880569). Can mediate the antiviral effect via the classical RNASEL-dependent pathway or an alternative antiviral pathway independent of RNASEL (PubMed:21142819). In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation (PubMed:21142819). May act as a negative regulator of lactation, stopping lactation in virally infected mammary gland lobules, thereby preventing transmission of viruses to neonates (By similarity). Non-infected lobules would not be affected, allowing efficient pup feeding during infection (By similarity).1 PublicationBy similarity4 Publications
Catalytic activityi
- EC:2.7.7.844 Publications
Cofactori
Mg2+1 Publication
Activity regulationi
Produced as a latent enzyme which is activated by double stranded RNA (dsRNA) generated during the course of viral infection(PubMed:9880569). The dsRNA activator must be at least 15 nucleotides long, and no modification of the 2'-hydroxyl group is tolerated (PubMed:9880569). ssRNA or dsDNA do not act as activators (PubMed:9880569). Strongly inhibited by copper, iron and zinc ions (PubMed:11682059). Partially inhibited by cobalt and nickel ions (PubMed:11682059).2 Publications
Kineticsi
- KM=2.1 mM for ATP3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 396 | ATPBy similarity | 1 | |
Metal bindingi | 408 | Magnesium; catalyticSequence analysis | 1 | |
Metal bindingi | 410 | Magnesium; catalyticSequence analysis | 1 | |
Metal bindingi | 481 | Magnesium; catalyticSequence analysis | 1 | |
Binding sitei | 544 | ATP1 Publication | 1 | |
Binding sitei | 547 | ATP1 Publication | 1 |
GO - Molecular functioni
- 2'-5'-oligoadenylate synthetase activity Source: UniProtKB
- ATP binding Source: UniProtKB
- double-stranded RNA binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: UniProtKB
- interferon-gamma-mediated signaling pathway Source: Reactome
- negative regulation of viral genome replication Source: GO_Central
- nucleobase-containing compound metabolic process Source: ProtInc
- regulation of lactation Source: UniProtKB
- regulation of ribonuclease activity Source: GO_Central
- response to bacterium Source: Ensembl
- response to virus Source: UniProtKB
- RNA catabolic process Source: Ensembl
- type I interferon signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase |
Biological process | Antiviral defense, Immunity, Innate immunity |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000111335-MONOMER |
PathwayCommonsi | P29728 |
Reactomei | R-HSA-877300, Interferon gamma signaling R-HSA-8983711, OAS antiviral response R-HSA-909733, Interferon alpha/beta signaling |
SABIO-RKi | P29728 |
Names & Taxonomyi
Protein namesi | Recommended name: 2'-5'-oligoadenylate synthase 2 (EC:2.7.7.843 Publications)Short name: (2-5')oligo(A) synthase 2 Short name: 2-5A synthase 2 Alternative name(s): p69 OAS / p71 OAS1 Publication Short name: p69OAS / p71OAS1 Publication |
Gene namesi | Name:OAS2Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8087, OAS2 |
MIMi | 603350, gene |
neXtProti | NX_P29728 |
VEuPathDBi | HostDB:ENSG00000111335.12 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- perinuclear region 1 Publication
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: ProtInc
- membrane Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A or D: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 408 | D → A: Loss of activity; when associated with A-410. 1 Publication | 1 | |
Mutagenesisi | 410 | D → A: Loss of activity; when associated with A-408. 1 Publication | 1 | |
Mutagenesisi | 421 | Y → P or A: Significant loss of activity. 1 Publication | 1 | |
Mutagenesisi | 481 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 544 | R → A or Y: Significant loss of activity. 1 Publication | 1 | |
Mutagenesisi | 547 | K → A: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 668 | C → A: Loss of activity; when associated with A-669 and A-670. 1 Publication | 1 | |
Mutagenesisi | 669 | F → A: Loss of activity; when associated with A-668 and A-670. 1 Publication | 1 | |
Mutagenesisi | 670 | K → A: Loss of activity; when associated with A-668 and A-669. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 4939 |
OpenTargetsi | ENSG00000111335 |
PharmGKBi | PA31876 |
Miscellaneous databases
Pharosi | P29728, Tbio |
Genetic variation databases
BioMutai | OAS2 |
DMDMi | 116242687 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000160264 | 2 – 719 | 2'-5'-oligoadenylate synthase 2Add BLAST | 718 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Modified residuei | 378 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Myristoylation is not essential for its activity.2 Publications
Glycosylated. Glycosylation is essential for its activity.1 Publication
Keywords - PTMi
Acetylation, Glycoprotein, Lipoprotein, MyristateProteomic databases
EPDi | P29728 |
jPOSTi | P29728 |
MassIVEi | P29728 |
MaxQBi | P29728 |
PaxDbi | P29728 |
PeptideAtlasi | P29728 |
PRIDEi | P29728 |
ProteomicsDBi | 54606 [P29728-1] 54607 [P29728-2] 54608 [P29728-3] |
PTM databases
iPTMneti | P29728 |
PhosphoSitePlusi | P29728 |
Expressioni
Inductioni
By type I interferon (IFN) and viruses.1 Publication
Gene expression databases
Bgeei | ENSG00000111335, Expressed in leukocyte and 186 other tissues |
ExpressionAtlasi | P29728, baseline and differential |
Genevisiblei | P29728, HS |
Organism-specific databases
HPAi | ENSG00000111335, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsBinary interactionsi
Hide detailsP29728
With | #Exp. | IntAct |
---|---|---|
CLK3 [P49761] | 4 | EBI-10211452,EBI-745579 |
Isoform p69 [P29728-2]
With | #Exp. | IntAct |
---|---|---|
CLK3 [P49761] | 5 | EBI-12270678,EBI-745579 |
FBXO17 [Q96EF6] | 3 | EBI-12270678,EBI-2510157 |
Protein-protein interaction databases
BioGRIDi | 110993, 8 interactors |
IntActi | P29728, 5 interactors |
STRINGi | 9606.ENSP00000342278 |
Miscellaneous databases
RNActi | P29728, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 11 – 335 | OAS domain 1Add BLAST | 325 | |
Regioni | 343 – 683 | OAS domain 2Add BLAST | 341 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 326 – 346 | Pro-rich (linker)Add BLAST | 21 |
Sequence similaritiesi
Belongs to the 2-5A synthase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502S649, Eukaryota |
GeneTreei | ENSGT00510000046406 |
HOGENOMi | CLU_026275_0_0_1 |
OrthoDBi | 611234at2759 |
PhylomeDBi | P29728 |
TreeFami | TF329749 |
Family and domain databases
CDDi | cd05400, NT_2-5OAS_ClassI-CCAase, 2 hits |
Gene3Di | 1.10.1410.20, 2 hits 3.30.460.10, 2 hits |
InterProi | View protein in InterPro IPR018952, 2-5-oligoAdlate_synth_1_dom2/C IPR038121, 2-5-oligoAdlate_synth_2_sf IPR026774, 2-5A_synthase IPR006117, 2-5OAS_C_CS IPR043518, 2-5OAS_N_CS IPR006116, NT_2-5OAS_ClassI-CCAase IPR043519, NT_sf IPR002934, Polymerase_NTP_transf_dom |
PANTHERi | PTHR11258, PTHR11258, 1 hit |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF10421, OAS1_C, 2 hits |
SUPFAMi | SSF81301, SSF81301, 2 hits |
PROSITEi | View protein in PROSITE PS00832, 25A_SYNTH_1, 2 hits PS00833, 25A_SYNTH_2, 2 hits PS50152, 25A_SYNTH_3, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform p711 Publication (identifier: P29728-1) [UniParc]FASTAAdd to basket
Also known as: 71 kDa1 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNGESQLSS VPAQKLGWFI QEYLKPYEEC QTLIDEMVNT ICDVLQEPEQ
60 70 80 90 100
FPLVQGVAIG GSYGRKTVLR GNSDGTLVLF FSDLKQFQDQ KRSQRDILDK
110 120 130 140 150
TGDKLKFCLF TKWLKNNFEI QKSLDGFTIQ VFTKNQRISF EVLAAFNALS
160 170 180 190 200
LNDNPSPWIY RELKRSLDKT NASPGEFAVC FTELQQKFFD NRPGKLKDLI
210 220 230 240 250
LLIKHWHQQC QKKIKDLPSL SPYALELLTV YAWEQGCRKD NFDIAEGVRT
260 270 280 290 300
VLELIKCQEK LCIYWMVNYN FEDETIRNIL LHQLQSARPV ILDPVDPTNN
310 320 330 340 350
VSGDKICWQW LKKEAQTWLT SPNLDNELPA PSWNVLPAPL FTTPGHLLDK
360 370 380 390 400
FIKEFLQPNK CFLEQIDSAV NIIRTFLKEN CFRQSTAKIQ IVRGGSTAKG
410 420 430 440 450
TALKTGSDAD LVVFHNSLKS YTSQKNERHK IVKEIHEQLK AFWREKEEEL
460 470 480 490 500
EVSFEPPKWK APRVLSFSLK SKVLNESVSF DVLPAFNALG QLSSGSTPSP
510 520 530 540 550
EVYAGLIDLY KSSDLPGGEF STCFTVLQRN FIRSRPTKLK DLIRLVKHWY
560 570 580 590 600
KECERKLKPK GSLPPKYALE LLTIYAWEQG SGVPDFDTAE GFRTVLELVT
610 620 630 640 650
QYQQLCIFWK VNYNFEDETV RKFLLSQLQK TRPVILDPAE PTGDVGGGDR
660 670 680 690 700
WCWHLLAKEA KEWLSSPCFK DGTGNPIPPW KVPTMQTPGS CGARIHPIVN
710
EMFSSRSHRI LNNNSKRNF
Isoform p692 Publications (identifier: P29728-2) [UniParc]FASTAAdd to basket
Also known as: 69 kDa2 Publications
The sequence of this isoform differs from the canonical sequence as follows:
684-687: TMQT → VKVI
688-719: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087X0V5 | A0A087X0V5_HUMAN | 2'-5' oligoadenylate synthase | OAS2 | 726 | Annotation score: | ||
F8W1C5 | F8W1C5_HUMAN | 2'-5'-oligoadenylate synthase 2 | OAS2 | 270 | Annotation score: | ||
F8VPI5 | F8VPI5_HUMAN | 2'-5'-oligoadenylate synthase 2 | OAS2 | 60 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 45 – 47 | LQE → CRN in AAA60607 (PubMed:1577824).Curated | 3 | |
Sequence conflicti | 45 – 47 | LQE → CRN in AAA60606 (PubMed:1577824).Curated | 3 | |
Sequence conflicti | 127 | F → S in AAA60607 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 127 | F → S in AAA60606 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 606 | C → G in AAA60607 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 606 | C → G in AAA60606 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 639 | A → G in AAA60607 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 639 | A → G in AAA60606 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 657 | A → D in AAA60607 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 657 | A → D in AAA60606 (PubMed:1577824).Curated | 1 | |
Sequence conflicti | 662 – 663 | EW → VR in AAA60607 (PubMed:1577824).Curated | 2 | |
Sequence conflicti | 662 – 663 | EW → VR in AAA60606 (PubMed:1577824).Curated | 2 | |
Sequence conflicti | 719 | F → FWRSSGNRF in AAA60607 (PubMed:1577824).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043354 | 151 – 172 | LNDNP…DKTNA → KHCWVSGEKSQRSGCQTALC NL in isoform 3. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_043355 | 173 – 719 | Missing in isoform 3. 1 PublicationAdd BLAST | 547 | |
Alternative sequenceiVSP_003741 | 684 – 687 | TMQT → VKVI in isoform p69. 3 Publications | 4 | |
Alternative sequenceiVSP_003742 | 688 – 719 | Missing in isoform p69. 3 PublicationsAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87434 mRNA Translation: AAA60607.1 M87284 mRNA Translation: AAA60606.1 AK292796 mRNA Translation: BAF85485.1 AC004551 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW98027.1 BC023637 mRNA Translation: AAH23637.1 BC049215 mRNA Translation: AAH49215.1 |
CCDSi | CCDS31906.1 [P29728-1] CCDS41839.1 [P29728-2] CCDS44982.1 [P29728-3] |
PIRi | B42665 |
RefSeqi | NP_001027903.1, NM_001032731.1 [P29728-3] NP_002526.2, NM_002535.2 [P29728-2] NP_058197.2, NM_016817.2 [P29728-1] |
Genome annotation databases
Ensembli | ENST00000342315; ENSP00000342278; ENSG00000111335 [P29728-1] ENST00000392583; ENSP00000376362; ENSG00000111335 [P29728-2] ENST00000449768; ENSP00000411763; ENSG00000111335 [P29728-3] |
GeneIDi | 4939 |
KEGGi | hsa:4939 |
UCSCi | uc001tuh.4, human [P29728-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87434 mRNA Translation: AAA60607.1 M87284 mRNA Translation: AAA60606.1 AK292796 mRNA Translation: BAF85485.1 AC004551 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW98027.1 BC023637 mRNA Translation: AAH23637.1 BC049215 mRNA Translation: AAH49215.1 |
CCDSi | CCDS31906.1 [P29728-1] CCDS41839.1 [P29728-2] CCDS44982.1 [P29728-3] |
PIRi | B42665 |
RefSeqi | NP_001027903.1, NM_001032731.1 [P29728-3] NP_002526.2, NM_002535.2 [P29728-2] NP_058197.2, NM_016817.2 [P29728-1] |
3D structure databases
SMRi | P29728 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110993, 8 interactors |
IntActi | P29728, 5 interactors |
STRINGi | 9606.ENSP00000342278 |
PTM databases
iPTMneti | P29728 |
PhosphoSitePlusi | P29728 |
Genetic variation databases
BioMutai | OAS2 |
DMDMi | 116242687 |
Proteomic databases
EPDi | P29728 |
jPOSTi | P29728 |
MassIVEi | P29728 |
MaxQBi | P29728 |
PaxDbi | P29728 |
PeptideAtlasi | P29728 |
PRIDEi | P29728 |
ProteomicsDBi | 54606 [P29728-1] 54607 [P29728-2] 54608 [P29728-3] |
Protocols and materials databases
Antibodypediai | 31214, 412 antibodies |
Genome annotation databases
Ensembli | ENST00000342315; ENSP00000342278; ENSG00000111335 [P29728-1] ENST00000392583; ENSP00000376362; ENSG00000111335 [P29728-2] ENST00000449768; ENSP00000411763; ENSG00000111335 [P29728-3] |
GeneIDi | 4939 |
KEGGi | hsa:4939 |
UCSCi | uc001tuh.4, human [P29728-1] |
Organism-specific databases
CTDi | 4939 |
DisGeNETi | 4939 |
GeneCardsi | OAS2 |
HGNCi | HGNC:8087, OAS2 |
HPAi | ENSG00000111335, Low tissue specificity |
MIMi | 603350, gene |
neXtProti | NX_P29728 |
OpenTargetsi | ENSG00000111335 |
PharmGKBi | PA31876 |
VEuPathDBi | HostDB:ENSG00000111335.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S649, Eukaryota |
GeneTreei | ENSGT00510000046406 |
HOGENOMi | CLU_026275_0_0_1 |
OrthoDBi | 611234at2759 |
PhylomeDBi | P29728 |
TreeFami | TF329749 |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000111335-MONOMER |
PathwayCommonsi | P29728 |
Reactomei | R-HSA-877300, Interferon gamma signaling R-HSA-8983711, OAS antiviral response R-HSA-909733, Interferon alpha/beta signaling |
SABIO-RKi | P29728 |
Miscellaneous databases
BioGRID-ORCSi | 4939, 7 hits in 994 CRISPR screens |
ChiTaRSi | OAS2, human |
GeneWikii | OAS2 |
GenomeRNAii | 4939 |
Pharosi | P29728, Tbio |
PROi | PR:P29728 |
RNActi | P29728, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111335, Expressed in leukocyte and 186 other tissues |
ExpressionAtlasi | P29728, baseline and differential |
Genevisiblei | P29728, HS |
Family and domain databases
CDDi | cd05400, NT_2-5OAS_ClassI-CCAase, 2 hits |
Gene3Di | 1.10.1410.20, 2 hits 3.30.460.10, 2 hits |
InterProi | View protein in InterPro IPR018952, 2-5-oligoAdlate_synth_1_dom2/C IPR038121, 2-5-oligoAdlate_synth_2_sf IPR026774, 2-5A_synthase IPR006117, 2-5OAS_C_CS IPR043518, 2-5OAS_N_CS IPR006116, NT_2-5OAS_ClassI-CCAase IPR043519, NT_sf IPR002934, Polymerase_NTP_transf_dom |
PANTHERi | PTHR11258, PTHR11258, 1 hit |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF10421, OAS1_C, 2 hits |
SUPFAMi | SSF81301, SSF81301, 2 hits |
PROSITEi | View protein in PROSITE PS00832, 25A_SYNTH_1, 2 hits PS00833, 25A_SYNTH_2, 2 hits PS50152, 25A_SYNTH_3, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OAS2_HUMAN | |
Accessioni | P29728Primary (citable) accession number: P29728 Secondary accession number(s): A8K9T1, Q6PJ33, Q86XX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 183 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families