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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85NucleophileBy similarity1
Active sitei172By similarity1
Active sitei174By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi227 – 233ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processGMP biosynthesis, Purine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU06360-MONOMER
UniPathwayiUPA00189; UER00296

Protein family/group databases

MEROPSiC26.957

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
Ordered Locus Names:BSU06360
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001400931 – 513GMP synthase [glutamine-hydrolyzing]Add BLAST513

Proteomic databases

PRIDEiP29727

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP29727, 2 interactors
MINTiP29727

Structurei

3D structure databases

ProteinModelPortaliP29727
SMRiP29727
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 198Glutamine amidotransferase type-1Add BLAST191
Domaini199 – 388GMPS ATP-PPaseAdd BLAST190

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000223964
InParanoidiP29727
KOiK01951
OMAiKRKIIGH
PhylomeDBiP29727

Family and domain databases

CDDicd01742 GATase1_GMP_Synthase, 1 hit
cd01997 GMP_synthase_C, 1 hit
Gene3Di3.40.50.620, 1 hit
3.40.50.880, 1 hit
HAMAPiMF_00344 GMP_synthase, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR001674 GMP_synth_C
IPR004739 GMP_synth_GATase
IPR022955 GMP_synthase
IPR025777 GMPS_ATP_PPase_dom
IPR022310 NAD/GMP_synthase
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00958 GMP_synt_C, 1 hit
PF02540 NAD_synthase, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00884 guaA_Cterm, 1 hit
TIGR00888 guaA_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS51553 GMPS_ATP_PPASE, 1 hit

Sequencei

Sequence statusi: Complete.

P29727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLVNEMIL VLDFGSQYNQ LITRRIREFG VYSELHPHTL TAEEIKKMNP
60 70 80 90 100
KGIILSGGPN SVYDENSFRC DEKIFELDIP VLGICYGMQL MTHYLGGKVE
110 120 130 140 150
AASQREYGKA NIRIEGTPDL FRDLPNEQVV WMSHGDLVVE VPEGFTVDAT
160 170 180 190 200
SHHCPNSAMS KADKKWYGVQ FHPEVRHSEY GNDLLKNFVF GVCECEGEWS
210 220 230 240 250
MENFIEIEMQ KIRETVGDKQ VLCALSGGVD SSVVAVLIHK AIGDQLTCIF
260 270 280 290 300
VDHGLLRKGE AEGVMKTFSE GFNMNVIKVD AKDRFLNKLK GVSDPEQKRK
310 320 330 340 350
IIGNEFIYVF DDEADKLKGI DYLAQGTLYT DIIESGTATA QTIKSHHNVG
360 370 380 390 400
GLPEDMQFEL IEPLNTLFKD EVRALGTELG IPDEIVWRQP FPGPGLGIRV
410 420 430 440 450
LGEVTEEKLE IVRESDAILR EEIANHGLER DIWQYFTVLP DIRSVGVMGD
460 470 480 490 500
ARTYDYTIGI RAVTSIDGMT SDWARIPWDV LEVISTRIVN EVKHINRVVY
510
DITSKPPATI EWE
Length:513
Mass (Da):57,849
Last modified:June 16, 2009 - v3
Checksum:iBBD6426FE37D41BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103S → T in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti149A → AA in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti149A → AA in AAB62311 (PubMed:8969499).Curated1
Sequence conflicti162A → G in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti167Y → H in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti195C → S in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti215T → Q in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti224A → G in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti261A → R in AAA22497 (PubMed:1312531).Curated1
Sequence conflicti459 – 463GIRAV → ESRR in AAA22497 (PubMed:1312531).Curated5
Sequence conflicti459 – 463GIRAV → ESRR in AAB62311 (PubMed:8969499).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83691 Genomic DNA Translation: AAA22497.1
U51115 Genomic DNA Translation: AAB62311.1
AL009126 Genomic DNA Translation: CAB12455.2
PIRiC69638
RefSeqiNP_388517.2, NC_000964.3
WP_003244399.1, NZ_JNCM01000032.1

Genome annotation databases

EnsemblBacteriaiCAB12455; CAB12455; BSU06360
GeneIDi936028
KEGGibsu:BSU06360
PATRICifig|224308.179.peg.691

Similar proteinsi

Entry informationi

Entry nameiGUAA_BACSU
AccessioniPrimary (citable) accession number: P29727
Secondary accession number(s): O34531
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 16, 2009
Last modified: February 28, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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