UniProtKB - P29704 (FDFT_YEAST)
Protein
Squalene synthase
Gene
ERG9
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. May also have a regulatory role regulating the flux of isoprene intermediates through the sterol pathway. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. ERG9 is also essential for cell growth in yeast.3 Publications
Miscellaneous
Present with 10800 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Cofactori
Mg2+1 Publication
: lanosterol biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Squalene synthase (ERG9)
- Squalene monooxygenase (ERG1)
- Lanosterol synthase (ERG7)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.
GO - Molecular functioni
- farnesyl-diphosphate farnesyltransferase activity Source: SGD
- squalene synthase activity Source: SGD
GO - Biological processi
- ergosterol biosynthetic process Source: SGD
- farnesyl diphosphate metabolic process Source: GO_Central
- isoprenoid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Multifunctional enzyme, Transferase |
Biological process | Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Magnesium, NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:YHR190W-MONOMER |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
UniPathwayi | UPA00767;UER00751 |
Names & Taxonomyi
Protein namesi | Recommended name: Squalene synthase (EC:2.5.1.21)Short name: SQS Short name: SS Alternative name(s): FPP:FPP farnesyltransferase Farnesyl-diphosphate farnesyltransferase |
Gene namesi | Name:ERG9 Ordered Locus Names:YHR190W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001233, ERG9 |
VEuPathDBi | FungiDB:YHR190W |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
- Microsome 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: GO_Central
Mitochondrion
- mitochondrial outer membrane Source: SGD
- mitochondrion Source: SGD
Other locations
- integral component of membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 291 – 311 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 421 – 441 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000067454 | 1 – 444 | Squalene synthaseAdd BLAST | 444 |
Proteomic databases
MaxQBi | P29704 |
PaxDbi | P29704 |
PRIDEi | P29704 |
PTM databases
iPTMneti | P29704 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 36624, 353 interactors |
DIPi | DIP-4576N |
IntActi | P29704, 9 interactors |
MINTi | P29704 |
STRINGi | 4932.YHR190W |
Miscellaneous databases
RNActi | P29704, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the phytoene/squalene synthase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1459, Eukaryota |
GeneTreei | ENSGT00390000016034 |
HOGENOMi | CLU_031981_2_1_1 |
InParanoidi | P29704 |
OMAi | NGPNEKD |
Family and domain databases
CDDi | cd00683, Trans_IPPS_HH, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR002060, Squ/phyt_synthse IPR006449, Squal_synth-like IPR019845, Squalene/phytoene_synthase_CS IPR033904, Trans_IPPS_HH |
SFLDi | SFLDG01018, Squalene/Phytoene_Synthase_Lik, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
TIGRFAMsi | TIGR01559, squal_synth, 1 hit |
PROSITEi | View protein in PROSITE PS01044, SQUALEN_PHYTOEN_SYN_1, 1 hit PS01045, SQUALEN_PHYTOEN_SYN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P29704-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKLLQLALH PVEMKAALKL KFCRTPLFSI YDQSTSPYLL HCFELLNLTS
60 70 80 90 100
RSFAAVIREL HPELRNCVTL FYLILRALDT IEDDMSIEHD LKIDLLRHFH
110 120 130 140 150
EKLLLTKWSF DGNAPDVKDR AVLTDFESIL IEFHKLKPEY QEVIKEITEK
160 170 180 190 200
MGNGMADYIL DENYNLNGLQ TVHDYDVYCH YVAGLVGDGL TRLIVIAKFA
210 220 230 240 250
NESLYSNEQL YESMGLFLQK TNIIRDYNED LVDGRSFWPK EIWSQYAPQL
260 270 280 290 300
KDFMKPENEQ LGLDCINHLV LNALSHVIDV LTYLAGIHEQ STFQFCAIPQ
310 320 330 340 350
VMAIATLALV FNNREVLHGN VKIRKGTTCY LILKSRTLRG CVEIFDYYLR
360 370 380 390 400
DIKSKLAVQD PNFLKLNIQI SKIEQFMEEM YQDKLPPNVK PNETPIFLKV
410 420 430 440
KERSRYDDEL VPTQQEEEYK FNMVLSIILS VLLGFYYIYT LHRA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 48 | L → F in CAA42583 (PubMed:1807826).Curated | 1 | |
Sequence conflicti | 286 | G → S in AAA34597 (PubMed:2068081).Curated | 1 | |
Sequence conflicti | 320 | N → D in CAA42583 (PubMed:1807826).Curated | 1 | |
Sequence conflicti | 330 | Y → C in CAA42583 (PubMed:1807826).Curated | 1 | |
Sequence conflicti | 429 | L → S in CAA42583 (PubMed:1807826).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63979 Genomic DNA Translation: AAA34597.1 X59959 Genomic DNA Translation: CAA42583.1 U00030 Genomic DNA Translation: AAB68360.1 BK006934 Genomic DNA Translation: DAA06882.1 |
PIRi | S46682 |
RefSeqi | NP_012060.1, NM_001179321.1 |
Genome annotation databases
EnsemblFungii | YHR190W_mRNA; YHR190W; YHR190W |
GeneIDi | 856597 |
KEGGi | sce:YHR190W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63979 Genomic DNA Translation: AAA34597.1 X59959 Genomic DNA Translation: CAA42583.1 U00030 Genomic DNA Translation: AAB68360.1 BK006934 Genomic DNA Translation: DAA06882.1 |
PIRi | S46682 |
RefSeqi | NP_012060.1, NM_001179321.1 |
3D structure databases
SMRi | P29704 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36624, 353 interactors |
DIPi | DIP-4576N |
IntActi | P29704, 9 interactors |
MINTi | P29704 |
STRINGi | 4932.YHR190W |
Chemistry databases
ChEMBLi | CHEMBL3271930 |
PTM databases
iPTMneti | P29704 |
Proteomic databases
MaxQBi | P29704 |
PaxDbi | P29704 |
PRIDEi | P29704 |
Genome annotation databases
EnsemblFungii | YHR190W_mRNA; YHR190W; YHR190W |
GeneIDi | 856597 |
KEGGi | sce:YHR190W |
Organism-specific databases
SGDi | S000001233, ERG9 |
VEuPathDBi | FungiDB:YHR190W |
Phylogenomic databases
eggNOGi | KOG1459, Eukaryota |
GeneTreei | ENSGT00390000016034 |
HOGENOMi | CLU_031981_2_1_1 |
InParanoidi | P29704 |
OMAi | NGPNEKD |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00751 |
BioCyci | MetaCyc:YHR190W-MONOMER |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
Miscellaneous databases
PROi | PR:P29704 |
RNActi | P29704, protein |
Family and domain databases
CDDi | cd00683, Trans_IPPS_HH, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR002060, Squ/phyt_synthse IPR006449, Squal_synth-like IPR019845, Squalene/phytoene_synthase_CS IPR033904, Trans_IPPS_HH |
SFLDi | SFLDG01018, Squalene/Phytoene_Synthase_Lik, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
TIGRFAMsi | TIGR01559, squal_synth, 1 hit |
PROSITEi | View protein in PROSITE PS01044, SQUALEN_PHYTOEN_SYN_1, 1 hit PS01045, SQUALEN_PHYTOEN_SYN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FDFT_YEAST | |
Accessioni | P29704Primary (citable) accession number: P29704 Secondary accession number(s): D3DLD8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names