UniProtKB - P29703 (FNTA_YEAST)
Protein
Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
Gene
RAM2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Essential subunit of both the farnesyltransferase and the geranylgeranyltransferase complex. Contributes to the transfer of a farnesyl or geranylgeranyl moiety from farnesyl or geranylgeranyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. If X is Ser, Ala, Met, Cys, or Gln, then the protein is farnesylated, if X is Leu or Phe, then the protein is geranylgeranylated. The alpha subunit wraps partly around the beta subunit forming part of the active site. The beta subunit is responsible for isoprenoid and peptide-binding.5 Publications
Miscellaneous
Present with 396 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- (2E,6E)-farnesyl diphosphate + L-cysteinyl-[protein] = diphosphate + S-(2E,6E)-farnesyl-L-cysteinyl-[protein]3 PublicationsEC:2.5.1.583 PublicationsThis reaction proceeds in the forward3 Publications direction.
- geranylgeranyl diphosphate + L-cysteinyl-[protein] = diphosphate + S-geranylgeranyl-L-cysteinyl-[protein]3 PublicationsEC:2.5.1.593 PublicationsThis reaction proceeds in the forward3 Publications direction.
Cofactori
Mg2+2 Publications
GO - Molecular functioni
- CAAX-protein geranylgeranyltransferase activity Source: SGD
- protein farnesyltransferase activity Source: SGD
- protein geranylgeranyltransferase activity Source: SGD
GO - Biological processi
- peptide pheromone maturation Source: SGD
- protein farnesylation Source: UniProtKB
- protein geranylgeranylation Source: SGD
Keywordsi
Molecular function | Prenyltransferase, Transferase |
Ligand | Magnesium |
Enzyme and pathway databases
Reactomei | R-SCE-9648002, RAS processing |
Names & Taxonomyi
Protein namesi | Recommended name: Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (EC:2.5.1.583 Publications, EC:2.5.1.593 Publications)Short name: PFTase/PGGTase I alpha Alternative name(s): CAAX farnesyltransferase subunit alpha FTase-alpha Ras proteins prenyltransferase subunit alpha Type I protein geranyl-geranyltransferase subunit alpha Short name: GGTase-I-alpha |
Gene namesi | Name:RAM2 Ordered Locus Names:YKL019W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YKL019W |
SGDi | S000001502, RAM2 |
Subcellular locationi
Other locations
- Cytoplasm Curated
Other locations
- CAAX-protein geranylgeranyltransferase complex Source: SGD
- cytoplasm Source: GO_Central
- protein farnesyltransferase complex Source: SGD
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 140 | D → N: Decreases catalytic activity 5-fold, but retains substrate binding properties. 1 Publication | 1 | |
Mutagenesisi | 143 | N → K in ram2-1; completely abolishes farnesyltransferase activity, but still has about 30% geranyl-geranyl transferase activity. 2 Publications | 1 | |
Mutagenesisi | 145 | H → D: Increases KM for peptide substrate 4-fold, but retains catalytic activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2406 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119754 | 1 – 316 | Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alphaAdd BLAST | 316 |
Proteomic databases
MaxQBi | P29703 |
PaxDbi | P29703 |
PRIDEi | P29703 |
Interactioni
Subunit structurei
Farnesyltransferase and geranyl-geranyltransferase share a common alpha subunit (RAM2) (Probable). Protein farnesyltransferase is a heterodimer of alpha subunit RAM2 and beta subunit RAM1 (PubMed:1763050). Protein geranyl-geranyltransferase type-1 is a heterodimer of alpha subunit RAM2 and beta subunit CDC43 (PubMed:1400380, PubMed:9174352).
1 Publication3 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 34112, 136 interactors |
ComplexPortali | CPX-1634, Protein farnesyltransferase complex CPX-1635, Protein geranylgeranyltransferase type I complex |
DIPi | DIP-1233N |
IntActi | P29703, 6 interactors |
MINTi | P29703 |
STRINGi | 4932.YKL019W |
Chemistry databases
BindingDBi | P29703 |
Miscellaneous databases
RNActi | P29703, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 47 – 81 | PFTA 1PROSITE-ProRule annotationAdd BLAST | 35 | |
Repeati | 89 – 123 | PFTA 2PROSITE-ProRule annotationAdd BLAST | 35 | |
Repeati | 125 – 159 | PFTA 3PROSITE-ProRule annotationAdd BLAST | 35 | |
Repeati | 160 – 193 | PFTA 4PROSITE-ProRule annotationAdd BLAST | 34 | |
Repeati | 199 – 233 | PFTA 5PROSITE-ProRule annotationAdd BLAST | 35 |
Sequence similaritiesi
Belongs to the protein prenyltransferase subunit alpha family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0530, Eukaryota |
GeneTreei | ENSGT00550000074935 |
HOGENOMi | CLU_026582_1_0_1 |
InParanoidi | P29703 |
OMAi | KGNESAW |
Family and domain databases
InterProi | View protein in InterPro IPR002088, Prenyl_trans_a |
Pfami | View protein in Pfam PF01239, PPTA, 4 hits |
PROSITEi | View protein in PROSITE PS51147, PFTA, 5 hits |
i Sequence
Sequence statusi: Complete.
P29703-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEEYDYSDVK PLPIETDLQD ELCRIMYTED YKRLMGLARA LISLNELSPR
60 70 80 90 100
ALQLTAEIID VAPAFYTIWN YRFNIVRHMM SESEDTVLYL NKELDWLDEV
110 120 130 140 150
TLNNPKNYQI WSYRQSLLKL HPSPSFKREL PILKLMIDDD SKNYHVWSYR
160 170 180 190 200
KWCCLFFSDF QHELAYASDL IETDIYNNSA WTHRMFYWVN AKDVISKVEL
210 220 230 240 250
ADELQFIMDK IQLVPQNISP WTYLRGFQEL FHDRLQWDSK VVDFATTFIG
260 270 280 290 300
DVLSLPIGSP EDLPEIESSY ALEFLAYHWG ADPCTRDNAV KAYSLLAIKY
310
DPIRKNLWHH KINNLN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88584 Genomic DNA Translation: AAA34957.1 Z28019 Genomic DNA Translation: CAA81854.1 L24760 Genomic DNA Translation: AAA20574.1 BK006944 Genomic DNA Translation: DAA09136.1 |
PIRi | A41626 |
RefSeqi | NP_012906.3, NM_001179585.3 |
Genome annotation databases
EnsemblFungii | YKL019W_mRNA; YKL019W; YKL019W |
GeneIDi | 853849 |
KEGGi | sce:YKL019W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88584 Genomic DNA Translation: AAA34957.1 Z28019 Genomic DNA Translation: CAA81854.1 L24760 Genomic DNA Translation: AAA20574.1 BK006944 Genomic DNA Translation: DAA09136.1 |
PIRi | A41626 |
RefSeqi | NP_012906.3, NM_001179585.3 |
3D structure databases
SMRi | P29703 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34112, 136 interactors |
ComplexPortali | CPX-1634, Protein farnesyltransferase complex CPX-1635, Protein geranylgeranyltransferase type I complex |
DIPi | DIP-1233N |
IntActi | P29703, 6 interactors |
MINTi | P29703 |
STRINGi | 4932.YKL019W |
Chemistry databases
BindingDBi | P29703 |
ChEMBLi | CHEMBL2406 |
Proteomic databases
MaxQBi | P29703 |
PaxDbi | P29703 |
PRIDEi | P29703 |
Genome annotation databases
EnsemblFungii | YKL019W_mRNA; YKL019W; YKL019W |
GeneIDi | 853849 |
KEGGi | sce:YKL019W |
Organism-specific databases
EuPathDBi | FungiDB:YKL019W |
SGDi | S000001502, RAM2 |
Phylogenomic databases
eggNOGi | KOG0530, Eukaryota |
GeneTreei | ENSGT00550000074935 |
HOGENOMi | CLU_026582_1_0_1 |
InParanoidi | P29703 |
OMAi | KGNESAW |
Enzyme and pathway databases
Reactomei | R-SCE-9648002, RAS processing |
Miscellaneous databases
PROi | PR:P29703 |
RNActi | P29703, protein |
Family and domain databases
InterProi | View protein in InterPro IPR002088, Prenyl_trans_a |
Pfami | View protein in Pfam PF01239, PPTA, 4 hits |
PROSITEi | View protein in PROSITE PS51147, PFTA, 5 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FNTA_YEAST | |
Accessioni | P29703Primary (citable) accession number: P29703 Secondary accession number(s): D6VXR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD