UniProtKB - P29692 (EF1D_HUMAN)
Protein
Elongation factor 1-delta
Gene
EEF1D
Organism
Homo sapiens (Human)
Status
Functioni
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
GO - Molecular functioni
- activating transcription factor binding Source: UniProtKB
- cadherin binding Source: BHF-UCL
- DNA binding Source: UniProtKB
- guanyl-nucleotide exchange factor activity Source: GO_Central
- heat shock protein binding Source: UniProtKB
- translation elongation factor activity Source: UniProtKB-KW
- translation factor activity, RNA binding Source: ProtInc
GO - Biological processi
- cellular response to ionizing radiation Source: UniProtKB
- mRNA transcription Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- regulation of cell death Source: UniProtKB
- translational elongation Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Elongation factor |
Biological process | Protein biosynthesis, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P29692 |
Reactomei | R-HSA-156842, Eukaryotic Translation Elongation |
SIGNORi | P29692 |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 1-deltaShort name: EF-1-delta Alternative name(s): Antigen NY-CO-4 |
Gene namesi | Name:EEF1D Synonyms:EF1D |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3211, EEF1D |
MIMi | 130592, gene |
neXtProti | NX_P29692 |
VEuPathDBi | HostDB:ENSG00000104529.17 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- fibrillar center Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- eukaryotic translation elongation factor 1 complex Source: ProtInc
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 1936 |
MalaCardsi | EEF1D |
OpenTargetsi | ENSG00000104529 |
PharmGKBi | PA27647 |
Miscellaneous databases
Pharosi | P29692, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295739 |
Genetic variation databases
BioMutai | EEF1D |
DMDMi | 20141357 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000155046 | 2 – 281 | Elongation factor 1-deltaAdd BLAST | 280 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | ||
Modified residuei | 17 | N6-acetyllysineCombined sources | 1 | ||
Modified residuei | 37 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 44 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 60 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 73 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 86 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 106 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 107 | N6-acetyllysineCombined sources | 1 | ||
Modified residuei | 117 | N6-acetyllysine; alternateCombined sources | 1 | ||
Modified residuei | 117 | N6-succinyllysine; alternateBy similarity | 1 | ||
Modified residuei | 119 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 129 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 133 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 147 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 162 | Phosphoserine; by CK2Combined sources1 Publication | 1 | ||
Isoform 3 (identifier: P29692-3) | |||||
Modified residuei | 40 | PhosphoserineCombined sources | 1 | ||
Isoform 2 (identifier: P29692-2) | |||||
Modified residuei | 91 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 94 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-500 CPTAC-501 |
EPDi | P29692 |
jPOSTi | P29692 |
MassIVEi | P29692 |
MaxQBi | P29692 |
PaxDbi | P29692 |
PeptideAtlasi | P29692 |
PRIDEi | P29692 |
ProteomicsDBi | 19276 54603 [P29692-1] 54604 [P29692-2] 54605 [P29692-3] |
TopDownProteomicsi | P29692-1 [P29692-1] P29692-3 [P29692-3] P29692-4 [P29692-4] |
2D gel databases
OGPi | P29692 |
PTM databases
iPTMneti | P29692 |
MetOSitei | P29692 |
PhosphoSitePlusi | P29692 |
SwissPalmi | P29692 |
Expressioni
Tissue specificityi
Isoform 2 is specifically expressed in brain, cerebellum and testis.
Inductioni
By homocysteine (HC), may mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress. Also induced following exposure to ionizing radiation.2 Publications
Gene expression databases
Bgeei | ENSG00000104529, Expressed in tendon of biceps brachii and 246 other tissues |
ExpressionAtlasi | P29692, baseline and differential |
Genevisiblei | P29692, HS |
Organism-specific databases
HPAi | ENSG00000104529, Low tissue specificity |
Interactioni
Subunit structurei
EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma.
Isoform 2 interacts with HSF1 and NFE2L2.
Binary interactionsi
Hide detailsP29692
Isoform 2 [P29692-2]
GO - Molecular functioni
- activating transcription factor binding Source: UniProtKB
- cadherin binding Source: BHF-UCL
- heat shock protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 108256, 217 interactors |
IntActi | P29692, 144 interactors |
MINTi | P29692 |
STRINGi | 9606.ENSP00000410059 |
Miscellaneous databases
RNActi | P29692, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P29692 |
SMRi | P29692 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 115 | Leucine-zipperAdd BLAST | 36 | |
Regioni | 173 – 281 | Catalytic (GEF)Add BLAST | 109 |
Sequence similaritiesi
Belongs to the EF-1-beta/EF-1-delta family.Curated
Phylogenomic databases
eggNOGi | KOG1668, Eukaryota |
GeneTreei | ENSGT00950000183014 |
HOGENOMi | CLU_020166_0_0_1 |
InParanoidi | P29692 |
OMAi | GFYEAMF |
OrthoDBi | 1464823at2759 |
PhylomeDBi | P29692 |
TreeFami | TF313134 |
Family and domain databases
CDDi | cd00292, EF1B, 1 hit |
Gene3Di | 3.30.70.60, 1 hit |
InterProi | View protein in InterPro IPR036219, eEF-1beta-like_sf IPR018940, EF-1_beta_acid_region_euk IPR014038, EF1B_bsu/dsu_GNE IPR014717, Transl_elong_EF1B/ribosomal_S6 IPR001326, Transl_elong_EF1B_B/D_CS |
Pfami | View protein in Pfam PF10587, EF-1_beta_acid, 1 hit PF00736, EF1_GNE, 1 hit |
SMARTi | View protein in SMART SM01182, EF-1_beta_acid, 1 hit SM00888, EF1_GNE, 1 hit |
SUPFAMi | SSF54984, SSF54984, 1 hit |
PROSITEi | View protein in PROSITE PS00824, EF1BD_1, 1 hit PS00825, EF1BD_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 39 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P29692-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI
60 70 80 90 100
ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE
110 120 130 140 150
LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE
160 170 180 190 200
DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL
210 220 230 240 250
DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE
260 270 280
DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I
Computationally mapped potential isoform sequencesi
There are 39 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PRY8 | E9PRY8_HUMAN | Elongation factor 1-delta | EEF1D | 697 | Annotation score: | ||
A0A087X1X7 | A0A087X1X7_HUMAN | Elongation factor 1-delta | EEF1D | 631 | Annotation score: | ||
E9PN71 | E9PN71_HUMAN | Elongation factor 1-delta | EEF1D | 190 | Annotation score: | ||
E9PRL0 | E9PRL0_HUMAN | Elongation factor 1-delta | EEF1D | 257 | Annotation score: | ||
E9PJV8 | E9PJV8_HUMAN | Elongation factor 1-delta | EEF1D | 179 | Annotation score: | ||
E9PPY1 | E9PPY1_HUMAN | Elongation factor 1-delta | EEF1D | 157 | Annotation score: | ||
E9PQR8 | E9PQR8_HUMAN | Elongation factor 1-delta | EEF1D | 300 | Annotation score: | ||
E9PN56 | E9PN56_HUMAN | Elongation factor 1-delta | EEF1D | 180 | Annotation score: | ||
E9PQZ1 | E9PQZ1_HUMAN | Elongation factor 1-delta | EEF1D | 238 | Annotation score: | ||
E9PK01 | E9PK01_HUMAN | Elongation factor 1-delta | EEF1D | 261 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 34 | A → R in CAA79716 (PubMed:8334168).Curated | 1 | ||
Sequence conflicti | 44 | S → T in CAA79716 (PubMed:8334168).Curated | 1 | ||
Sequence conflicti | 209 | T → A in BAG56855 (PubMed:14702039).Curated | 1 | ||
Isoform 2 (identifier: P29692-2) | |||||
Sequence conflicti | 189 | D → E in AAP35906 (Ref. 3) Curated | 1 | ||
Sequence conflicti | 189 | D → E in EAW82228 (PubMed:14702039).Curated | 1 | ||
Sequence conflicti | 189 | D → E in AAH07847 (PubMed:16421571).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037884 | 1 | M → MRSGKASCTLETVWEDKHKY EEAERRFYEHEATQAAASAQ QLPAEGPAMNGPGQDDPEDA DEAEAPDGGSRRDPRKSQDS RKPLQKKRKRSPKSGLGPAD LALLGLSAERVWLDKSLFDQ AESSYRQKLADVAAQAAWPP ALAPWGLCTHGNQVACHHVT WGIWVNKSSFDQAERAFVEW SQALLLAPDGSRRQGTPNTG QQVAVPDLAHQPSPPVNGQP PLGSLQALVREVWLEKPRYD AAERGFYEALFDGHPPGKVR LQERAGLAEGARRGRRDRRG RNILGNKRAGLRRADGEAPS ALPYCYFLQKDAEAPWLSKP AYDSAECRHHAAEALRVAWC LEAASLSHRPGPRSGLSVSS LRPNRKM in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_043812 | 40 – 63 | Missing in isoform 3. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_045960 | 78 – 96 | Missing in isoform 4. 1 PublicationAdd BLAST | 19 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MVM | NMR | - | A | 153-192 | [»] | |
2MVN | NMR | - | A | 153-192 | [»] | |
2N51 | NMR | - | A | 153-281 | [»] | |
5JPO | X-ray | 2.00 | E | 1-30 | [»] | |
BMRBi | P29692 | |||||
SMRi | P29692 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108256, 217 interactors |
IntActi | P29692, 144 interactors |
MINTi | P29692 |
STRINGi | 9606.ENSP00000410059 |
Chemistry databases
ChEMBLi | CHEMBL4295739 |
PTM databases
iPTMneti | P29692 |
MetOSitei | P29692 |
PhosphoSitePlusi | P29692 |
SwissPalmi | P29692 |
Genetic variation databases
BioMutai | EEF1D |
DMDMi | 20141357 |
2D gel databases
OGPi | P29692 |
Proteomic databases
CPTACi | CPTAC-500 CPTAC-501 |
EPDi | P29692 |
jPOSTi | P29692 |
MassIVEi | P29692 |
MaxQBi | P29692 |
PaxDbi | P29692 |
PeptideAtlasi | P29692 |
PRIDEi | P29692 |
ProteomicsDBi | 19276 54603 [P29692-1] 54604 [P29692-2] 54605 [P29692-3] |
TopDownProteomicsi | P29692-1 [P29692-1] P29692-3 [P29692-3] P29692-4 [P29692-4] |
Protocols and materials databases
Antibodypediai | 27948, 303 antibodies |
DNASUi | 1936 |
Genome annotation databases
Organism-specific databases
CTDi | 1936 |
DisGeNETi | 1936 |
GeneCardsi | EEF1D |
HGNCi | HGNC:3211, EEF1D |
HPAi | ENSG00000104529, Low tissue specificity |
MalaCardsi | EEF1D |
MIMi | 130592, gene |
neXtProti | NX_P29692 |
OpenTargetsi | ENSG00000104529 |
PharmGKBi | PA27647 |
VEuPathDBi | HostDB:ENSG00000104529.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1668, Eukaryota |
GeneTreei | ENSGT00950000183014 |
HOGENOMi | CLU_020166_0_0_1 |
InParanoidi | P29692 |
OMAi | GFYEAMF |
OrthoDBi | 1464823at2759 |
PhylomeDBi | P29692 |
TreeFami | TF313134 |
Enzyme and pathway databases
PathwayCommonsi | P29692 |
Reactomei | R-HSA-156842, Eukaryotic Translation Elongation |
SIGNORi | P29692 |
Miscellaneous databases
BioGRID-ORCSi | 1936, 10 hits in 997 CRISPR screens |
ChiTaRSi | EEF1D, human |
GeneWikii | EEF1D |
GenomeRNAii | 1936 |
Pharosi | P29692, Tbio |
PROi | PR:P29692 |
RNActi | P29692, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000104529, Expressed in tendon of biceps brachii and 246 other tissues |
ExpressionAtlasi | P29692, baseline and differential |
Genevisiblei | P29692, HS |
Family and domain databases
CDDi | cd00292, EF1B, 1 hit |
Gene3Di | 3.30.70.60, 1 hit |
InterProi | View protein in InterPro IPR036219, eEF-1beta-like_sf IPR018940, EF-1_beta_acid_region_euk IPR014038, EF1B_bsu/dsu_GNE IPR014717, Transl_elong_EF1B/ribosomal_S6 IPR001326, Transl_elong_EF1B_B/D_CS |
Pfami | View protein in Pfam PF10587, EF-1_beta_acid, 1 hit PF00736, EF1_GNE, 1 hit |
SMARTi | View protein in SMART SM01182, EF-1_beta_acid, 1 hit SM00888, EF1_GNE, 1 hit |
SUPFAMi | SSF54984, SSF54984, 1 hit |
PROSITEi | View protein in PROSITE PS00824, EF1BD_1, 1 hit PS00825, EF1BD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EF1D_HUMAN | |
Accessioni | P29692Primary (citable) accession number: P29692 Secondary accession number(s): B4DDU4 Q96I38 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 221 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families