UniProtKB - P29692 (EF1D_HUMAN)
Protein
Elongation factor 1-delta
Gene
EEF1D
Organism
Homo sapiens (Human)
Status
Functioni
Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
GO - Molecular functioni
- activating transcription factor binding Source: UniProtKB
- cadherin binding Source: BHF-UCL
- DNA binding Source: UniProtKB
- guanyl-nucleotide exchange factor activity Source: GO_Central
- heat shock protein binding Source: UniProtKB
- translation elongation factor activity Source: UniProtKB-KW
- translation factor activity, RNA binding Source: ProtInc
GO - Biological processi
- cellular response to ionizing radiation Source: UniProtKB
- mRNA transcription Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- regulation of cell death Source: UniProtKB
- translational elongation Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Elongation factor |
Biological process | Protein biosynthesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-HSA-156842 Eukaryotic Translation Elongation |
SIGNORi | P29692 |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 1-deltaShort name: EF-1-delta Alternative name(s): Antigen NY-CO-4 |
Gene namesi | Name:EEF1D Synonyms:EF1D |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3211 EEF1D |
MIMi | 130592 gene |
neXtProti | NX_P29692 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- fibrillar center Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- eukaryotic translation elongation factor 1 complex Source: ProtInc
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 1936 |
MalaCardsi | EEF1D |
OpenTargetsi | ENSG00000104529 |
PharmGKBi | PA27647 |
Miscellaneous databases
Pharosi | P29692 |
Polymorphism and mutation databases
BioMutai | EEF1D |
DMDMi | 20141357 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000155046 | 2 – 281 | Elongation factor 1-deltaAdd BLAST | 280 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | ||
Modified residuei | 17 | N6-acetyllysineCombined sources | 1 | ||
Modified residuei | 37 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 44 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 60 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 73 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 86 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 106 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 107 | N6-acetyllysineCombined sources | 1 | ||
Modified residuei | 117 | N6-acetyllysine; alternateCombined sources | 1 | ||
Modified residuei | 117 | N6-succinyllysine; alternateBy similarity | 1 | ||
Modified residuei | 119 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 129 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 133 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 147 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 162 | Phosphoserine; by CK2Combined sources1 Publication | 1 | ||
Isoform 3 (identifier: P29692-3) | |||||
Modified residuei | 40 | PhosphoserineCombined sources | 1 | ||
Isoform 2 (identifier: P29692-2) | |||||
Modified residuei | 91 | PhosphoserineCombined sourcesCurated | 1 | ||
Modified residuei | 94 | PhosphoserineCombined sourcesCurated | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-500 CPTAC-501 |
EPDi | P29692 |
jPOSTi | P29692 |
MassIVEi | P29692 |
MaxQBi | P29692 |
PaxDbi | P29692 |
PeptideAtlasi | P29692 |
PRIDEi | P29692 |
ProteomicsDBi | 19276 54603 [P29692-1] 54604 [P29692-2] 54605 [P29692-3] |
TopDownProteomicsi | P29692-1 [P29692-1] P29692-3 [P29692-3] P29692-4 [P29692-4] |
2D gel databases
OGPi | P29692 |
PTM databases
iPTMneti | P29692 |
PhosphoSitePlusi | P29692 |
SwissPalmi | P29692 |
Expressioni
Tissue specificityi
Isoform 2 is specifically expressed in brain, cerebellum and testis.
Inductioni
By homocysteine (HC), may mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress. Also induced following exposure to ionizing radiation.2 Publications
Gene expression databases
Bgeei | ENSG00000104529 Expressed in 236 organ(s), highest expression level in tendon of biceps brachii |
ExpressionAtlasi | P29692 baseline and differential |
Genevisiblei | P29692 HS |
Organism-specific databases
HPAi | HPA045101 HPA051002 |
Interactioni
Subunit structurei
EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma.
Isoform 2 interacts with HSF1 and NFE2L2.
Binary interactionsi
GO - Molecular functioni
- activating transcription factor binding Source: UniProtKB
- cadherin binding Source: BHF-UCL
- guanyl-nucleotide exchange factor activity Source: GO_Central
- heat shock protein binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 108256, 174 interactors |
IntActi | P29692, 127 interactors |
MINTi | P29692 |
STRINGi | 9606.ENSP00000410059 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P29692 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 115 | Leucine-zipperAdd BLAST | 36 | |
Regioni | 173 – 281 | Catalytic (GEF)Add BLAST | 109 |
Sequence similaritiesi
Belongs to the EF-1-beta/EF-1-delta family.Curated
Phylogenomic databases
eggNOGi | KOG1668 Eukaryota COG2092 LUCA |
GeneTreei | ENSGT00950000183014 |
HOGENOMi | HOG000207272 |
InParanoidi | P29692 |
KOi | K15410 |
OrthoDBi | 1464823at2759 |
PhylomeDBi | P29692 |
TreeFami | TF313134 |
Family and domain databases
CDDi | cd00292 EF1B, 1 hit |
Gene3Di | 3.30.70.60, 1 hit |
InterProi | View protein in InterPro IPR036219 eEF-1beta-like_sf IPR018940 EF-1_beta_acid_region_euk IPR014038 EF1B_bsu/dsu_GNE IPR014717 Transl_elong_EF1B/ribosomal_S6 IPR001326 Transl_elong_EF1B_B/D_CS |
Pfami | View protein in Pfam PF10587 EF-1_beta_acid, 1 hit PF00736 EF1_GNE, 1 hit |
SMARTi | View protein in SMART SM01182 EF-1_beta_acid, 1 hit SM00888 EF1_GNE, 1 hit |
SUPFAMi | SSF54984 SSF54984, 1 hit |
PROSITEi | View protein in PROSITE PS00824 EF1BD_1, 1 hit PS00825 EF1BD_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 39 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P29692-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI
60 70 80 90 100
ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE
110 120 130 140 150
LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE
160 170 180 190 200
DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL
210 220 230 240 250
DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE
260 270 280
DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I
Computationally mapped potential isoform sequencesi
There are 39 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PRY8 | E9PRY8_HUMAN | Elongation factor 1-delta | EEF1D | 697 | Annotation score: | ||
A0A087X1X7 | A0A087X1X7_HUMAN | Elongation factor 1-delta | EEF1D | 631 | Annotation score: | ||
E9PJV8 | E9PJV8_HUMAN | Elongation factor 1-delta | EEF1D | 179 | Annotation score: | ||
E9PN71 | E9PN71_HUMAN | Elongation factor 1-delta | EEF1D | 190 | Annotation score: | ||
E9PPY1 | E9PPY1_HUMAN | Elongation factor 1-delta | EEF1D | 157 | Annotation score: | ||
E9PQR8 | E9PQR8_HUMAN | Elongation factor 1-delta | EEF1D | 300 | Annotation score: | ||
E9PRL0 | E9PRL0_HUMAN | Elongation factor 1-delta | EEF1D | 257 | Annotation score: | ||
E9PN56 | E9PN56_HUMAN | Elongation factor 1-delta | EEF1D | 180 | Annotation score: | ||
E9PQZ1 | E9PQZ1_HUMAN | Elongation factor 1-delta | EEF1D | 238 | Annotation score: | ||
H0YE72 | H0YE72_HUMAN | Elongation factor 1-delta | EEF1D | 156 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 34 | A → R in CAA79716 (PubMed:8334168).Curated | 1 | ||
Sequence conflicti | 44 | S → T in CAA79716 (PubMed:8334168).Curated | 1 | ||
Sequence conflicti | 209 | T → A in BAG56855 (PubMed:14702039).Curated | 1 | ||
Isoform 2 (identifier: P29692-2) | |||||
Sequence conflicti | 189 | D → E in AAP35906 (Ref. 3) Combined sourcesCurated | 1 | ||
Sequence conflicti | 189 | D → E in EAW82228 (PubMed:14702039).Combined sourcesCurated | 1 | ||
Sequence conflicti | 189 | D → E in AAH07847 (PubMed:16421571).Combined sourcesCurated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037884 | 1 | M → MRSGKASCTLETVWEDKHKY EEAERRFYEHEATQAAASAQ QLPAEGPAMNGPGQDDPEDA DEAEAPDGGSRRDPRKSQDS RKPLQKKRKRSPKSGLGPAD LALLGLSAERVWLDKSLFDQ AESSYRQKLADVAAQAAWPP ALAPWGLCTHGNQVACHHVT WGIWVNKSSFDQAERAFVEW SQALLLAPDGSRRQGTPNTG QQVAVPDLAHQPSPPVNGQP PLGSLQALVREVWLEKPRYD AAERGFYEALFDGHPPGKVR LQERAGLAEGARRGRRDRRG RNILGNKRAGLRRADGEAPS ALPYCYFLQKDAEAPWLSKP AYDSAECRHHAAEALRVAWC LEAASLSHRPGPRSGLSVSS LRPNRKM in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_043812 | 40 – 63 | Missing in isoform 3. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_045960 | 78 – 96 | Missing in isoform 4. 1 PublicationAdd BLAST | 19 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MVM | NMR | - | A | 153-192 | [»] | |
2MVN | NMR | - | A | 153-192 | [»] | |
2N51 | NMR | - | A | 153-281 | [»] | |
5JPO | X-ray | 2.00 | E | 1-30 | [»] | |
SMRi | P29692 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 108256, 174 interactors |
IntActi | P29692, 127 interactors |
MINTi | P29692 |
STRINGi | 9606.ENSP00000410059 |
PTM databases
iPTMneti | P29692 |
PhosphoSitePlusi | P29692 |
SwissPalmi | P29692 |
Polymorphism and mutation databases
BioMutai | EEF1D |
DMDMi | 20141357 |
2D gel databases
OGPi | P29692 |
Proteomic databases
CPTACi | CPTAC-500 CPTAC-501 |
EPDi | P29692 |
jPOSTi | P29692 |
MassIVEi | P29692 |
MaxQBi | P29692 |
PaxDbi | P29692 |
PeptideAtlasi | P29692 |
PRIDEi | P29692 |
ProteomicsDBi | 19276 54603 [P29692-1] 54604 [P29692-2] 54605 [P29692-3] |
TopDownProteomicsi | P29692-1 [P29692-1] P29692-3 [P29692-3] P29692-4 [P29692-4] |
Protocols and materials databases
DNASUi | 1936 |
Genome annotation databases
Organism-specific databases
CTDi | 1936 |
DisGeNETi | 1936 |
GeneCardsi | EEF1D |
HGNCi | HGNC:3211 EEF1D |
HPAi | HPA045101 HPA051002 |
MalaCardsi | EEF1D |
MIMi | 130592 gene |
neXtProti | NX_P29692 |
OpenTargetsi | ENSG00000104529 |
PharmGKBi | PA27647 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1668 Eukaryota COG2092 LUCA |
GeneTreei | ENSGT00950000183014 |
HOGENOMi | HOG000207272 |
InParanoidi | P29692 |
KOi | K15410 |
OrthoDBi | 1464823at2759 |
PhylomeDBi | P29692 |
TreeFami | TF313134 |
Enzyme and pathway databases
Reactomei | R-HSA-156842 Eukaryotic Translation Elongation |
SIGNORi | P29692 |
Miscellaneous databases
ChiTaRSi | EEF1D human |
GeneWikii | EEF1D |
GenomeRNAii | 1936 |
Pharosi | P29692 |
PROi | PR:P29692 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000104529 Expressed in 236 organ(s), highest expression level in tendon of biceps brachii |
ExpressionAtlasi | P29692 baseline and differential |
Genevisiblei | P29692 HS |
Family and domain databases
CDDi | cd00292 EF1B, 1 hit |
Gene3Di | 3.30.70.60, 1 hit |
InterProi | View protein in InterPro IPR036219 eEF-1beta-like_sf IPR018940 EF-1_beta_acid_region_euk IPR014038 EF1B_bsu/dsu_GNE IPR014717 Transl_elong_EF1B/ribosomal_S6 IPR001326 Transl_elong_EF1B_B/D_CS |
Pfami | View protein in Pfam PF10587 EF-1_beta_acid, 1 hit PF00736 EF1_GNE, 1 hit |
SMARTi | View protein in SMART SM01182 EF-1_beta_acid, 1 hit SM00888 EF1_GNE, 1 hit |
SUPFAMi | SSF54984 SSF54984, 1 hit |
PROSITEi | View protein in PROSITE PS00824 EF1BD_1, 1 hit PS00825 EF1BD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EF1D_HUMAN | |
Accessioni | P29692Primary (citable) accession number: P29692 Secondary accession number(s): B4DDU4 Q96I38 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | November 13, 2019 | |
This is version 212 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - SIMILARITY comments
Index of protein domains and families - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references