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Protein

Kallistatin

Gene

SERPINA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.1 Publication

Miscellaneous

Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei388 – 389Reactive bond2

GO - Molecular functioni

  • serine-type endopeptidase inhibitor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation

Protein family/group databases

MEROPSiI04.003

Names & Taxonomyi

Protein namesi
Recommended name:
Kallistatin
Alternative name(s):
Kallikrein inhibitor
Peptidase inhibitor 4
Short name:
PI-4
Serpin A4
Gene namesi
Name:SERPINA4
Synonyms:KST, PI4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100665.11
HGNCiHGNC:8948 SERPINA4
MIMi147935 gene
neXtProtiNX_P29622

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5267
OpenTargetsiENSG00000100665
PharmGKBiPA35514

Polymorphism and mutation databases

DMDMi68067608

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003242521 – 427KallistatinAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi108N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi157N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi238N-linked (GlcNAc...) (complex) asparagine2 Publications1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP29622
PeptideAtlasiP29622
PRIDEiP29622
ProteomicsDBi54602
TopDownProteomicsiP29622

PTM databases

GlyConnecti1429
iPTMnetiP29622
PhosphoSitePlusiP29622

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiENSG00000100665 Expressed in 45 organ(s), highest expression level in liver
ExpressionAtlasiP29622 baseline and differential
GenevisibleiP29622 HS

Organism-specific databases

HPAiHPA003607

Interactioni

Subunit structurei

Monomer and some homodimers.

Protein-protein interaction databases

BioGridi111285, 15 interactors
IntActiP29622, 10 interactors
MINTiP29622
STRINGi9606.ENSP00000298841

Structurei

3D structure databases

ProteinModelPortaliP29622
SMRiP29622
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392 Eukaryota
COG4826 LUCA
GeneTreeiENSGT00760000118839
HOGENOMiHOG000238521
HOVERGENiHBG005957
InParanoidiP29622
KOiK04525
OMAiQVPMMLQ
OrthoDBiEOG091G0ION
PhylomeDBiP29622
TreeFamiTF343201

Family and domain databases

InterProiView protein in InterPro
IPR023795 Serpin_CS
IPR023796 Serpin_dom
IPR000215 Serpin_fam
IPR036186 Serpin_sf
PANTHERiPTHR11461 PTHR11461, 1 hit
PfamiView protein in Pfam
PF00079 Serpin, 1 hit
SMARTiView protein in SMART
SM00093 SERPIN, 1 hit
SUPFAMiSSF56574 SSF56574, 1 hit
PROSITEiView protein in PROSITE
PS00284 SERPIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29622-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHLIDYLLLL LVGLLALSHG QLHVEHDGES CSNSSHQQIL ETGEGSPSLK
60 70 80 90 100
IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ
110 120 130 140 150
ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK
160 170 180 190 200
FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE
210 220 230 240 250
LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ
260 270 280 290 300
EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR
310 320 330 340 350
WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG
360 370 380 390 400
ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN
410 420
RPFLVVIFST STQSVLFLGK VVDPTKP
Length:427
Mass (Da):48,542
Last modified:June 21, 2005 - v3
Checksum:i68EBE7AF956BFB77
GO

Sequence cautioni

The sequence CAD66567 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382S → T (PubMed:8227002).Curated1
Sequence conflicti382S → T (PubMed:7835886).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19684 mRNA Translation: AAA59454.1
L28101 Genomic DNA Translation: AAC41706.1
BX248009 mRNA Translation: CAD62337.1
BX248760 mRNA Translation: CAD66567.1 Different initiation.
BC014992 mRNA Translation: AAH14992.1
CCDSiCCDS9927.1
PIRiA49518
RefSeqiNP_001275961.1, NM_001289032.1
NP_001275962.1, NM_001289033.1
NP_006206.2, NM_006215.3
UniGeneiHs.719893

Genome annotation databases

EnsembliENST00000298841; ENSP00000298841; ENSG00000100665
ENST00000555095; ENSP00000451172; ENSG00000100665
ENST00000557004; ENSP00000450838; ENSG00000100665
GeneIDi5267
KEGGihsa:5267
UCSCiuc001ydk.5 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19684 mRNA Translation: AAA59454.1
L28101 Genomic DNA Translation: AAC41706.1
BX248009 mRNA Translation: CAD62337.1
BX248760 mRNA Translation: CAD66567.1 Different initiation.
BC014992 mRNA Translation: AAH14992.1
CCDSiCCDS9927.1
PIRiA49518
RefSeqiNP_001275961.1, NM_001289032.1
NP_001275962.1, NM_001289033.1
NP_006206.2, NM_006215.3
UniGeneiHs.719893

3D structure databases

ProteinModelPortaliP29622
SMRiP29622
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111285, 15 interactors
IntActiP29622, 10 interactors
MINTiP29622
STRINGi9606.ENSP00000298841

Protein family/group databases

MEROPSiI04.003

PTM databases

GlyConnecti1429
iPTMnetiP29622
PhosphoSitePlusiP29622

Polymorphism and mutation databases

DMDMi68067608

Proteomic databases

PaxDbiP29622
PeptideAtlasiP29622
PRIDEiP29622
ProteomicsDBi54602
TopDownProteomicsiP29622

Protocols and materials databases

DNASUi5267
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298841; ENSP00000298841; ENSG00000100665
ENST00000555095; ENSP00000451172; ENSG00000100665
ENST00000557004; ENSP00000450838; ENSG00000100665
GeneIDi5267
KEGGihsa:5267
UCSCiuc001ydk.5 human

Organism-specific databases

CTDi5267
DisGeNETi5267
EuPathDBiHostDB:ENSG00000100665.11
GeneCardsiSERPINA4
HGNCiHGNC:8948 SERPINA4
HPAiHPA003607
MIMi147935 gene
neXtProtiNX_P29622
OpenTargetsiENSG00000100665
PharmGKBiPA35514
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2392 Eukaryota
COG4826 LUCA
GeneTreeiENSGT00760000118839
HOGENOMiHOG000238521
HOVERGENiHBG005957
InParanoidiP29622
KOiK04525
OMAiQVPMMLQ
OrthoDBiEOG091G0ION
PhylomeDBiP29622
TreeFamiTF343201

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation

Miscellaneous databases

GeneWikiiSERPINA4
GenomeRNAii5267
PROiPR:P29622
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100665 Expressed in 45 organ(s), highest expression level in liver
ExpressionAtlasiP29622 baseline and differential
GenevisibleiP29622 HS

Family and domain databases

InterProiView protein in InterPro
IPR023795 Serpin_CS
IPR023796 Serpin_dom
IPR000215 Serpin_fam
IPR036186 Serpin_sf
PANTHERiPTHR11461 PTHR11461, 1 hit
PfamiView protein in Pfam
PF00079 Serpin, 1 hit
SMARTiView protein in SMART
SM00093 SERPIN, 1 hit
SUPFAMiSSF56574 SSF56574, 1 hit
PROSITEiView protein in PROSITE
PS00284 SERPIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKAIN_HUMAN
AccessioniPrimary (citable) accession number: P29622
Secondary accession number(s): Q53XB5, Q86TR9, Q96BZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 21, 2005
Last modified: November 7, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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