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Protein

Subtilisin Savinase

Gene
N/A
Organism
Bacillus lentus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Miscellaneous

Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2Calcium 11
Active sitei32Charge relay system1
Metal bindingi40Calcium 11
Active sitei62Charge relay system1
Metal bindingi73Calcium 1; via carbonyl oxygen1
Metal bindingi75Calcium 11
Metal bindingi77Calcium 1; via carbonyl oxygen1
Metal bindingi79Calcium 1; via carbonyl oxygen1
Metal bindingi163Calcium 2; via carbonyl oxygen1
Metal bindingi165Calcium 2; via carbonyl oxygen1
Metal bindingi168Calcium 2; via carbonyl oxygen1
Active sitei215Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processSporulation
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.62 668

Protein family/group databases

MEROPSiS08.003

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin Savinase (EC:3.4.21.62)
Alternative name(s):
Alkaline protease
OrganismiBacillus lentus
Taxonomic identifieri1467 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

Used as a detergent protease. Sold under the name Savinase by Novozymes.

Protein family/group databases

Allergomei903 Bac l Subtilisin

Chemistry databases

DrugBankiDB01973 O-Benzylsulfonyl-Serine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764181 – 269Subtilisin SavinaseAdd BLAST269

Proteomic databases

PRIDEiP29600

Structurei

Secondary structure

1269
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP29600
SMRiP29600
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29600

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 268Peptidase S8Add BLAST242

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

P29600-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
AQSVPWGISR VQAPAAHNRG LTGSGVKVAV LDTGISTHPD LNIRGGASFV
60 70 80 90 100
PGEPSTQDGN GHGTHVAGTI AALNNSIGVL GVAPSAELYA VKVLGASGSG
110 120 130 140 150
SVSSIAQGLE WAGNNGMHVA NLSLGSPSPS ATLEQAVNSA TSRGVLVVAA
160 170 180 190 200
SGNSGAGSIS YPARYANAMA VGATDQNNNR ASFSQYGAGL DIVAPGVNVQ
210 220 230 240 250
STYPGSTYAS LNGTSMATPH VAGAAALVKQ KNPSWSNVQI RNHLKNTATS
260
LGSTNLYGSG LVNAEAATR
Length:269
Mass (Da):26,698
Last modified:April 1, 1993 - v1
Checksum:i4D89F8778999BF8D
GO

Similar proteinsi

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9JX-ray1.80A1-267[»]
1C9MX-ray1.67A1-269[»]
1C9NX-ray1.50A1-267[»]
1GCIX-ray0.78A1-269[»]
1IAVX-ray1.80A1-269[»]
1JEAX-ray2.00A1-269[»]
1NDQX-ray1.80A1-269[»]
1NDUX-ray1.60A1-269[»]
1Q5PX-ray1.60A1-269[»]
1SVNX-ray1.40A1-269[»]
1TK2X-ray1.54A1-263[»]
3BX1X-ray1.85A/B1-269[»]
4CFYX-ray1.17A1-269[»]
4CFZX-ray1.57A1-269[»]
4CG0X-ray1.36A1-269[»]
5AQEX-ray1.10A1-269[»]
5ARBX-ray1.15A1-269[»]
5ARCX-ray1.10A1-269[»]
5ARDX-ray1.55A1-269[»]
ProteinModelPortaliP29600
SMRiP29600
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB01973 O-Benzylsulfonyl-Serine

Protein family/group databases

Allergomei903 Bac l Subtilisin
MEROPSiS08.003

Proteomic databases

PRIDEiP29600

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.21.62 668

Miscellaneous databases

EvolutionaryTraceiP29600

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSUBS_BACLE
AccessioniPrimary (citable) accession number: P29600
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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