UniProtKB - P29598 (UROK_RAT)
Urokinase-type plasminogen activator
Plau
Functioni
Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
By similarityCatalytic activityi
- Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. EC:3.4.21.73
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 225 | Charge relay system | 1 | |
Active sitei | 276 | Charge relay system | 1 | |
Active sitei | 377 | Charge relay system | 1 |
GO - Molecular functioni
- peptidase activity Source: RGD
- serine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- angiogenesis Source: RGD
- cellular response to fluid shear stress Source: RGD
- cellular response to glucose stimulus Source: RGD
- cellular response to hepatocyte growth factor stimulus Source: RGD
- cellular response to hypoxia Source: RGD
- cellular response to lipopolysaccharide Source: RGD
- cellular response to organic substance Source: RGD
- cellular response to staurosporine Source: RGD
- embryo implantation Source: RGD
- fibrinolysis Source: RGD
- neuron death Source: RGD
- plasminogen activation Source: RGD
- positive regulation of cell migration Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of ovulation Source: RGD
- positive regulation of reactive oxygen species metabolic process Source: RGD
- positive regulation of smooth muscle cell migration Source: RGD
- proteolysis Source: GO_Central
- regulation of cell adhesion mediated by integrin Source: RGD
- regulation of cell population proliferation Source: RGD
- regulation of hepatocyte proliferation Source: RGD
- regulation of signaling receptor activity Source: RGD
- regulation of smooth muscle cell-matrix adhesion Source: RGD
- regulation of smooth muscle cell migration Source: RGD
- response to activity Source: RGD
- response to hepatocyte growth factor Source: RGD
- response to hyperoxia Source: RGD
- response to hypoxia Source: RGD
- skeletal muscle tissue regeneration Source: RGD
- smooth muscle cell migration Source: RGD
- spermatogenesis Source: RGD
- wound healing Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Plasminogen activation |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation R-RNO-75205, Dissolution of Fibrin Clot |
Protein family/group databases
MEROPSi | S01.231 |
Names & Taxonomyi
Protein namesi | Recommended name: Urokinase-type plasminogen activator (EC:3.4.21.73)Short name: U-plasminogen activator Short name: uPA Cleaved into the following 3 chains: |
Gene namesi | Name:Plau |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3343, Plau |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: RGD
Other locations
- cell surface Source: RGD
- membrane Source: RGD
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000028333 | 20 – 432 | Urokinase-type plasminogen activatorAdd BLAST | 413 | |
ChainiPRO_0000028334 | 20 – 177 | Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST | 158 | |
ChainiPRO_0000028335 | 156 – 177 | Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST | 22 | |
ChainiPRO_0000028336 | 179 – 432 | Urokinase-type plasminogen activator chain BBy similarityAdd BLAST | 254 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 39 | By similarity | ||
Disulfide bondi | 33 ↔ 51 | By similarity | ||
Disulfide bondi | 53 ↔ 62 | By similarity | ||
Disulfide bondi | 70 ↔ 151 | By similarity | ||
Disulfide bondi | 91 ↔ 133 | By similarity | ||
Disulfide bondi | 122 ↔ 146 | By similarity | ||
Modified residuei | 158 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 168 ↔ 300 | Interchain (between A and B chains)PROSITE-ProRule annotation | ||
Disulfide bondi | 210 ↔ 226 | By similarity | ||
Disulfide bondi | 218 ↔ 289 | By similarity | ||
Disulfide bondi | 314 ↔ 383 | By similarity | ||
Disulfide bondi | 346 ↔ 362 | By similarity | ||
Disulfide bondi | 373 ↔ 401 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Phosphoprotein, ZymogenProteomic databases
PaxDbi | P29598 |
PTM databases
PhosphoSitePlusi | P29598 |
Interactioni
Subunit structurei
Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation.
Interacts with MRC2.
Interacts with PLAUR. In complex with SERPINE1, interacts with PLAUR/uPAR.
Interacts with SORL1 and LRP1, either alone or in complex with SERPINE1; these interactions are abolished in the presence of LRPAP1/RAP. The ternary complex composed of PLAUR-PLAU-PAI1 also interacts with SORLA.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014273 |
Chemistry databases
BindingDBi | P29598 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 63 | EGF-likePROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 70 – 151 | KringlePROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 179 – 425 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 247 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 57 | Binds urokinase plasminogen activator surface receptorAdd BLAST | 24 | |
Regioni | 152 – 178 | Connecting peptideAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Kringle, SignalPhylogenomic databases
eggNOGi | ENOG502QRMI, Eukaryota |
InParanoidi | P29598 |
PhylomeDBi | P29598 |
Family and domain databases
CDDi | cd00108, KR, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR033116, TRYPSIN_SER IPR034814, Urokinase |
PANTHERi | PTHR24264:SF38, PTHR24264:SF38, 1 hit |
Pfami | View protein in Pfam PF00051, Kringle, 1 hit PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS50026, EGF_3, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MRVWLASLFL CALVANSEGG SELEASDESN CGCQNGGVCV SYKYFSSIRR
60 70 80 90 100
CSCPKKFKGE HCEIDTSKTC YHGNGQSYRG KANTDTKGRP CLAWNSPAVL
110 120 130 140 150
QQTYNAHRSD ALSLGLGKHN YCRNPDNQRR PWCYVQIGLK QFVQECMVQD
160 170 180 190 200
CSLSKKPSST VDQQGFQCGQ KALRPRFKIV GGEFTVVENQ PWFAAIYLKN
210 220 230 240 250
KGGSPPSFKC GGSLISPCWV ASATHCFVNQ PKKEEYVVYL GQSKRNSYNP
260 270 280 290 300
GEMKFEVEQL ILHEDFSDET LAFHNDIALL KIRTSTGQCA QPSRTIQTIC
310 320 330 340 350
LPPRFGDAPF GSDCEITGFG QESATDYFYP KDLKMSVVKI ISHEQCKQPH
360 370 380 390 400
YYGSEINYKM LCAADPEWKT DSCSGDSGGP LICNIDGRPT LSGIVSWGSG
410 420 430
CAEKNKPGVY TRVSYFLNWI QSHIGEENGL AF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ3KR76 | Q3KR76_RAT | Urokinase-type plasminogen activato... | Plau rCG_41849 | 432 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | N → H in CAA46601 (Ref. 2) Curated | 1 | |
Sequence conflicti | 24 | E → G in CAA46601 (Ref. 2) Curated | 1 | |
Sequence conflicti | 332 | D → N in CAA46601 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63434 mRNA Translation: CAA45028.1 X65651 mRNA Translation: CAA46601.1 X66907 Genomic DNA Translation: CAA47356.1 |
PIRi | S24604, S18932 |
Genome annotation databases
UCSCi | RGD:3343, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63434 mRNA Translation: CAA45028.1 X65651 mRNA Translation: CAA46601.1 X66907 Genomic DNA Translation: CAA47356.1 |
PIRi | S24604, S18932 |
3D structure databases
SMRi | P29598 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014273 |
Chemistry databases
BindingDBi | P29598 |
ChEMBLi | CHEMBL1075245 |
Protein family/group databases
MEROPSi | S01.231 |
PTM databases
PhosphoSitePlusi | P29598 |
Proteomic databases
PaxDbi | P29598 |
Genome annotation databases
UCSCi | RGD:3343, rat |
Organism-specific databases
RGDi | 3343, Plau |
Phylogenomic databases
eggNOGi | ENOG502QRMI, Eukaryota |
InParanoidi | P29598 |
PhylomeDBi | P29598 |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation R-RNO-75205, Dissolution of Fibrin Clot |
Miscellaneous databases
PROi | PR:P29598 |
Family and domain databases
CDDi | cd00108, KR, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR033116, TRYPSIN_SER IPR034814, Urokinase |
PANTHERi | PTHR24264:SF38, PTHR24264:SF38, 1 hit |
Pfami | View protein in Pfam PF00051, Kringle, 1 hit PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS50026, EGF_3, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UROK_RAT | |
Accessioni | P29598Primary (citable) accession number: P29598 Secondary accession number(s): Q6LBK5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | September 29, 2021 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families