UniProtKB - P29595 (NEDD8_MOUSE)
Protein
NEDD8
Gene
Nedd8
Organism
Mus musculus (Mouse)
Status
Functioni
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.1 Publication
GO - Molecular functioni
- protein tag Source: GO_Central
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- modification-dependent protein catabolic process Source: GO_Central
- protein localization Source: MGI
- protein neddylation Source: MGI
- regulation of proteolysis Source: GO_Central
- regulation of transcription by RNA polymerase II Source: MGI
- response to organic cyclic compound Source: MGI
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-2173789, TGF-beta receptor signaling activates SMADs R-MMU-5689603, UCH proteinases R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8951664, Neddylation R-MMU-917937, Iron uptake and transport |
Names & Taxonomyi
Protein namesi | Recommended name: NEDD8Alternative name(s): Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 Short name: NEDD-8 Ubiquitin-like protein Nedd8 |
Gene namesi | Name:Nedd8 Synonyms:Nedd-8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97301, Nedd8 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Mainly nuclear.
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000042769 | 1 – 76 | NEDD8Add BLAST | 76 | |
PropeptideiPRO_0000042770 | 77 – 81 | By similarity | 5 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 76 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) |
Post-translational modificationi
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.By similarity
Keywords - PTMi
Acetylation, Isopeptide bondProteomic databases
CPTACi | non-CPTAC-4051 |
EPDi | P29595 |
jPOSTi | P29595 |
PaxDbi | P29595 |
PeptideAtlasi | P29595 |
PRIDEi | P29595 |
PTM databases
iPTMneti | P29595 |
PhosphoSitePlusi | P29595 |
Expressioni
Tissue specificityi
Ubiquitously expressed (at protein level).2 Publications
Developmental stagei
According to PubMed:1378265 and PubMed:15183309, down-regulated during embryonic development, but according to PubMed:10597293, expression does not change during embryonic development. Between 10 dpc and 12 dpc, expressed in heart and spinal ganglia. Between 13.5 dpc and 16.5 dpc, strongly expressed in spinal and sympathetic ganglia, neural epithelium of the retina, oral and olfactory epithelia and thymus.4 Publications
Gene expression databases
Bgeei | ENSMUSG00000010376, Expressed in female gonad and 320 other tissues |
ExpressionAtlasi | P29595, baseline and differential |
Genevisiblei | P29595, MM |
Interactioni
Subunit structurei
Directly interacts with NUB1. Covalently attached to cullins and p53 (By similarity).
Interacts with AHR.
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 8 | Interaction with UBE1CBy similarity | 1 | |
Sitei | 44 | Interaction with UBE1CBy similarity | 1 |
Binary interactionsi
P29595
With | #Exp. | IntAct |
---|---|---|
sseL [A0A0F6B423] from Salmonella typhimurium (strain 14028s / SGSC 2262). | 2 | EBI-13953910,EBI-13953897 |
GO - Molecular functioni
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201725, 31 interactors |
IntActi | P29595, 2 interactors |
STRINGi | 10090.ENSMUSP00000010520 |
Miscellaneous databases
RNActi | P29595, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 70 – 72 | Interaction with UBE1CBy similarity | 3 |
Sequence similaritiesi
Belongs to the ubiquitin family.Curated
Phylogenomic databases
eggNOGi | KOG0005, Eukaryota |
GeneTreei | ENSGT00940000155856 |
HOGENOMi | CLU_010412_6_4_1 |
InParanoidi | P29595 |
OMAi | YGGKQMS |
OrthoDBi | 1536766at2759 |
PhylomeDBi | P29595 |
TreeFami | TF300072 |
Family and domain databases
CDDi | cd01806, Ubl_NEDD8, 1 hit |
InterProi | View protein in InterPro IPR038738, Nedd8-like IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom |
Pfami | View protein in Pfam PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P29595-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM
60 70 80
NDEKTAADYK ILGGSVLHLV LALRGGGGLG Q
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BRG0 | A0A2I3BRG0_MOUSE | NEDD8 | Nedd8 | 18 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10918 mRNA Translation: BAA01719.1 BC004625 mRNA Translation: AAH04625.1 |
CCDSi | CCDS49498.1 |
PIRi | JN0710 |
RefSeqi | NP_032709.1, NM_008683.3 |
Genome annotation databases
Ensembli | ENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376 ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376 |
GeneIDi | 18002 |
KEGGi | mmu:18002 |
UCSCi | uc007uac.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10918 mRNA Translation: BAA01719.1 BC004625 mRNA Translation: AAH04625.1 |
CCDSi | CCDS49498.1 |
PIRi | JN0710 |
RefSeqi | NP_032709.1, NM_008683.3 |
3D structure databases
BMRBi | P29595 |
SMRi | P29595 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201725, 31 interactors |
IntActi | P29595, 2 interactors |
STRINGi | 10090.ENSMUSP00000010520 |
PTM databases
iPTMneti | P29595 |
PhosphoSitePlusi | P29595 |
Proteomic databases
CPTACi | non-CPTAC-4051 |
EPDi | P29595 |
jPOSTi | P29595 |
PaxDbi | P29595 |
PeptideAtlasi | P29595 |
PRIDEi | P29595 |
Protocols and materials databases
Antibodypediai | 22753, 470 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376 ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376 |
GeneIDi | 18002 |
KEGGi | mmu:18002 |
UCSCi | uc007uac.1, mouse |
Organism-specific databases
CTDi | 4738 |
MGIi | MGI:97301, Nedd8 |
Phylogenomic databases
eggNOGi | KOG0005, Eukaryota |
GeneTreei | ENSGT00940000155856 |
HOGENOMi | CLU_010412_6_4_1 |
InParanoidi | P29595 |
OMAi | YGGKQMS |
OrthoDBi | 1536766at2759 |
PhylomeDBi | P29595 |
TreeFami | TF300072 |
Enzyme and pathway databases
Reactomei | R-MMU-2173789, TGF-beta receptor signaling activates SMADs R-MMU-5689603, UCH proteinases R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8951664, Neddylation R-MMU-917937, Iron uptake and transport |
Miscellaneous databases
BioGRID-ORCSi | 18002, 9 hits in 18 CRISPR screens |
ChiTaRSi | Nedd8, mouse |
PROi | PR:P29595 |
RNActi | P29595, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000010376, Expressed in female gonad and 320 other tissues |
ExpressionAtlasi | P29595, baseline and differential |
Genevisiblei | P29595, MM |
Family and domain databases
CDDi | cd01806, Ubl_NEDD8, 1 hit |
InterProi | View protein in InterPro IPR038738, Nedd8-like IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom |
Pfami | View protein in Pfam PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEDD8_MOUSE | |
Accessioni | P29595Primary (citable) accession number: P29595 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families