UniProtKB - P29595 (NEDD8_MOUSE)
NEDD8
Nedd8
Functioni
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:11696557).
Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (By similarity).
By similarity1 PublicationGO - Molecular functioni
- protein tag Source: GO_Central
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- modification-dependent protein catabolic process Source: GO_Central
- protein localization Source: MGI
- protein neddylation Source: MGI
- regulation of proteolysis Source: GO_Central
- regulation of transcription by RNA polymerase II Source: MGI
- response to organic cyclic compound Source: MGI
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-2173789, TGF-beta receptor signaling activates SMADs R-MMU-5689603, UCH proteinases R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8951664, Neddylation R-MMU-917937, Iron uptake and transport |
Names & Taxonomyi
Protein namesi | Recommended name: NEDD8Alternative name(s): Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 Short name: NEDD-8 Ubiquitin-like protein Nedd8 |
Gene namesi | Name:Nedd8 Synonyms:Nedd-8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97301, Nedd8 |
VEuPathDBi | HostDB:ENSMUSG00000010376 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Mainly nuclear.1 Publication
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000042769 | 1 – 76 | NEDD8Add BLAST | 76 | |
PropeptideiPRO_0000042770 | 77 – 81 | By similarity | 5 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 76 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bondProteomic databases
CPTACi | non-CPTAC-4051 |
EPDi | P29595 |
jPOSTi | P29595 |
PaxDbi | P29595 |
PeptideAtlasi | P29595 |
PRIDEi | P29595 |
ProteomicsDBi | 286177 |
PTM databases
iPTMneti | P29595 |
PhosphoSitePlusi | P29595 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000010376, Expressed in female gonad and 324 other tissues |
ExpressionAtlasi | P29595, baseline and differential |
Genevisiblei | P29595, MM |
Interactioni
Subunit structurei
Interacts with AHR; interaction is direct.
Interacts with NUB1; interaction is direct.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 8 | Interaction with UBE1CBy similarity | 1 | |
Sitei | 44 | Interaction with UBE1CBy similarity | 1 |
Binary interactionsi
P29595
With | #Exp. | IntAct |
---|---|---|
sseL [A0A0F6B423] from Salmonella typhimurium (strain 14028s / SGSC 2262). | 2 | EBI-13953910,EBI-13953897 |
GO - Molecular functioni
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201725, 31 interactors |
IntActi | P29595, 2 interactors |
STRINGi | 10090.ENSMUSP00000010520 |
Miscellaneous databases
RNActi | P29595, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 70 – 72 | Interaction with UBE1CBy similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0005, Eukaryota |
GeneTreei | ENSGT00940000155856 |
HOGENOMi | CLU_010412_6_4_1 |
InParanoidi | P29595 |
OMAi | VAHIKEK |
OrthoDBi | 1536766at2759 |
PhylomeDBi | P29595 |
TreeFami | TF300072 |
Family and domain databases
CDDi | cd01806, Ubl_NEDD8, 1 hit |
InterProi | View protein in InterPro IPR038738, Nedd8-like IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom |
Pfami | View protein in Pfam PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM
60 70 80
NDEKTAADYK ILGGSVLHLV LALRGGGGLG Q
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BRG0 | A0A2I3BRG0_MOUSE | NEDD8 | Nedd8 | 18 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10918 mRNA Translation: BAA01719.1 BC004625 mRNA Translation: AAH04625.1 |
CCDSi | CCDS49498.1 |
PIRi | JN0710 |
RefSeqi | NP_032709.1, NM_008683.3 |
Genome annotation databases
Ensembli | ENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376 ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376 |
GeneIDi | 18002 |
KEGGi | mmu:18002 |
UCSCi | uc007uac.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10918 mRNA Translation: BAA01719.1 BC004625 mRNA Translation: AAH04625.1 |
CCDSi | CCDS49498.1 |
PIRi | JN0710 |
RefSeqi | NP_032709.1, NM_008683.3 |
3D structure databases
BMRBi | P29595 |
SMRi | P29595 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201725, 31 interactors |
IntActi | P29595, 2 interactors |
STRINGi | 10090.ENSMUSP00000010520 |
PTM databases
iPTMneti | P29595 |
PhosphoSitePlusi | P29595 |
Proteomic databases
CPTACi | non-CPTAC-4051 |
EPDi | P29595 |
jPOSTi | P29595 |
PaxDbi | P29595 |
PeptideAtlasi | P29595 |
PRIDEi | P29595 |
ProteomicsDBi | 286177 |
Protocols and materials databases
Antibodypediai | 22753, 504 antibodies from 38 providers |
DNASUi | 18002 |
Genome annotation databases
Ensembli | ENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376 ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376 |
GeneIDi | 18002 |
KEGGi | mmu:18002 |
UCSCi | uc007uac.1, mouse |
Organism-specific databases
CTDi | 4738 |
MGIi | MGI:97301, Nedd8 |
VEuPathDBi | HostDB:ENSMUSG00000010376 |
Phylogenomic databases
eggNOGi | KOG0005, Eukaryota |
GeneTreei | ENSGT00940000155856 |
HOGENOMi | CLU_010412_6_4_1 |
InParanoidi | P29595 |
OMAi | VAHIKEK |
OrthoDBi | 1536766at2759 |
PhylomeDBi | P29595 |
TreeFami | TF300072 |
Enzyme and pathway databases
Reactomei | R-MMU-2173789, TGF-beta receptor signaling activates SMADs R-MMU-5689603, UCH proteinases R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8951664, Neddylation R-MMU-917937, Iron uptake and transport |
Miscellaneous databases
BioGRID-ORCSi | 18002, 24 hits in 60 CRISPR screens |
ChiTaRSi | Nedd8, mouse |
PROi | PR:P29595 |
RNActi | P29595, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000010376, Expressed in female gonad and 324 other tissues |
ExpressionAtlasi | P29595, baseline and differential |
Genevisiblei | P29595, MM |
Family and domain databases
CDDi | cd01806, Ubl_NEDD8, 1 hit |
InterProi | View protein in InterPro IPR038738, Nedd8-like IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom |
Pfami | View protein in Pfam PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NEDD8_MOUSE | |
Accessioni | P29595Primary (citable) accession number: P29595 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families