UniProtKB - P29539 (RIF1_YEAST)
Telomere length regulator protein RIF1
RIF1
Functioni
Negatively regulates telomere length by preventing telomere elongation or promoting degradation of the telomere ends. Recruited to telomeres by interaction with the C-terminus of RAP1, which binds directly to telomeric repeat DNA. This may create a negative feedback loop in which the addition of new telomere repeats creates binding sites for inhibitors of telomere length extension. May also influence the balance of transcriptional silencing at telomeres and the silent mating type locus HMR, which is mediated by SIR (Silent Information Regulator) proteins including SIR3 and SIR4. RIF1 competes with SIR proteins for binding to the C-terminus of RAP1. In the absence of RIF1, a limiting cellular pool of SIR proteins may preferentially associate with RAP1 at sub-telomeric loci, causing enhanced telomeric silencing and attenuated silencing of the HMR locus.
5 PublicationsGO - Molecular functioni
- telomeric DNA binding Source: SGD
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- DNA double-strand break processing Source: SGD
- DNA replication initiation Source: SGD
- negative regulation of DNA-dependent DNA replication initiation Source: SGD
- negative regulation of mitotic DNA replication initiation from late origin Source: SGD
- protein localization to chromosome, telomeric region Source: SGD
- regulation of DNA stability Source: SGD
- silent mating-type cassette heterochromatin assembly Source: SGD
- telomere capping Source: SGD
- telomere maintenance Source: SGD
Keywordsi
Biological process | Cell cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Telomere length regulator protein RIF1Alternative name(s): RAP1-interacting factor 1 |
Gene namesi | Name:RIF1 Ordered Locus Names:YBR275C ORF Names:YBR1743 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000479, RIF1 |
VEuPathDBi | FungiDB:YBR275C |
Subcellular locationi
Nucleus
Other locations
Note: Localizes to telomeres. Telomere association begins in G1/S, attains a peak during late G2/S phase of the cell cycle, and is lost during telophase. Localization to telomeres may be increased by telomere uncapping caused by expression of a mutant telomerase RNA subunit (TLC1).
Nucleus
- nucleus Source: GO_Central
- shelterin complex Source: SGD
Other locations
- chromosome, telomeric region Source: SGD
Keywords - Cellular componenti
Chromosome, Nucleus, TelomerePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097336 | 1 – 1916 | Telomere length regulator protein RIF1Add BLAST | 1916 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 97 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1637 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1795 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1852 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P29539 |
PaxDbi | P29539 |
PRIDEi | P29539 |
PTM databases
iPTMneti | P29539 |
SwissPalmi | P29539 |
Interactioni
Subunit structurei
Interacts with RIF2 and RAP1.
2 PublicationsBinary interactionsi
P29539
With | #Exp. | IntAct |
---|---|---|
RAP1 [P11938] | 3 | EBI-2083307,EBI-14821 |
Protein-protein interaction databases
BioGRIDi | 32970, 127 interactors |
ComplexPortali | CPX-2112, Telosome |
DIPi | DIP-801N |
IntActi | P29539, 8 interactors |
MINTi | P29539 |
STRINGi | 4932.YBR275C |
Miscellaneous databases
RNActi | P29539, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P29539 |
SMRi | P29539 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 71 | DisorderedSequence analysisAdd BLAST | 71 | |
Regioni | 126 – 149 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 1314 – 1372 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 1441 – 1476 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 1606 – 1678 | DisorderedSequence analysisAdd BLAST | 73 | |
Regioni | 1789 – 1811 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 1829 – 1854 | DisorderedSequence analysisAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 24 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 46 – 64 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 1314 – 1357 | Polar residuesSequence analysisAdd BLAST | 44 | |
Compositional biasi | 1441 – 1461 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 1462 – 1476 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1627 – 1647 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 1650 – 1668 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 1797 – 1811 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QPT7, Eukaryota |
HOGENOMi | CLU_002800_0_0_1 |
InParanoidi | P29539 |
OMAi | CDARRIN |
Family and domain databases
DisProti | DP01607 |
IDEALi | IID50206 |
InterProi | View protein in InterPro IPR028566, Rif1 IPR022031, Rif1_N |
PANTHERi | PTHR22928, PTHR22928, 1 hit |
Pfami | View protein in Pfam PF12231, Rif1_N, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKDFSDKKK HTIDRIDQHI LRRSQHDNYS NGSSPWMKTN LPPPSPQAHM
60 70 80 90 100
HIQSDLSPTP KRRKLASSSD CENKQFDLSA INKNLYPEDT GSRLMQSLPE
110 120 130 140 150
LSASNSDNVS PVTKSVAFSD RIESSPIYRI PGSSPKPSPS SKPGKSILRN
160 170 180 190 200
RLPSVRTVSD LSYNKLQYTQ HKLHNGNIFT SPYKETRVNP RALEYWVSGE
210 220 230 240 250
IHGLVDNESV SEFKEIIEGG LGILRQESED YVARRFEVYA TFNNIIPILT
260 270 280 290 300
TKNVNEVDQK FNILIVNIES IIEICIPHLQ IAQDTLLSSS EKKNPFVIRL
310 320 330 340 350
YVQIVRFFSA IMSNFKIVKW LTKRPDLVNK LKVIYRWTTG ALRNENSNKI
360 370 380 390 400
IITAQVSFLR DEKFGTFFLS NEEIKPIIST FTEIMEINSH NLIYEKLLLI
410 420 430 440 450
RGFLSKYPKL MIETVTSWLP GEVLPRIIIG DEIYSMKILI TSIVVLLELL
460 470 480 490 500
KKCLDFVDEH ERIYQCIMLS PVCETIPEKF LSKLPLNSYD SANLDKVTIG
510 520 530 540 550
HLLTQQIKNY IVVKNDNKIA MDLWLSMTGL LYDSGKRVYD LTSESNKVWF
560 570 580 590 600
DLNNLCFINN HPKTRLMSIK VWRIITYCIC TKISQKNQEG NKSLLSLLRT
610 620 630 640 650
PFQMTLPYVN DPSAREGIIY HLLGVVYTAF TSNKNLSTDM FELFWDHLIT
660 670 680 690 700
PIYEDYVFKY DSIHLQNVLF TVLHLLIGGK NADVALERKY KKHIHPMSVI
710 720 730 740 750
ASEGVKLKDI SSLPPQIIKR EYDKIMKVVF QAVEVAISNV NLAHDLILTS
760 770 780 790 800
LKHLPEDRKD QTHLESFSSL ILKVTQNNKD TPIFRDFFGA VTSSFVYTFL
810 820 830 840 850
DLFLRKNDSS LVNFNIQISK VGISQGNMTL DLLKDVIRKA RNETSEFLII
860 870 880 890 900
EKFLELDDKK TEVYAQNWVG STLLPPNISF REFQSLANIV NKVPNENSIE
910 920 930 940 950
NFLDLCLKLS FPVNLFTLLH VSMWSNNNFI YFIQSYVSKN ENKLNVDLIT
960 970 980 990 1000
LLKTSLPGNP ELFSGLLPFL RRNKFMDILE YCIHSNPNLL NSIPDLNSDL
1010 1020 1030 1040 1050
LLKLLPRSRA SYFAANIKLF KCSEQLTLVR WLLKGQQLEQ LNQNFSEIEN
1060 1070 1080 1090 1100
VLQNASDSEL EKSEIIRELL HLAMANPIEP LFSGLLNFCI KNNMADHLDE
1110 1120 1130 1140 1150
FCGNMTSEVL FKISPELLLK LLTYKEKPNG KLLAAVIEKI ENGDDDYILE
1160 1170 1180 1190 1200
LLEKIIIQKE IQILEKLKEP LLVFFLNPVS SNMQKHKKST NMLRELVLLY
1210 1220 1230 1240 1250
LTKPLSRSAA KKFFSMLISI LPPNPNYQTI DMVNLLIDLI KSHNRKFKDK
1260 1270 1280 1290 1300
RTYNATLKTI GKWIQESGVV HQGDSSKEIE AIPDTKSMYI PCEGSENKLS
1310 1320 1330 1340 1350
NLQRKVDSQD IQVPATQGMK EPPSSIQISS QISAKDSDSI SLKNTAIMNS
1360 1370 1380 1390 1400
SQQESHANRS RSIDDETLEE VDNESIREID QQMKSTQLDK NVANHSNICS
1410 1420 1430 1440 1450
TKSDEVDVTE LHESIDTQSS EVNAYQPIEV LTSELKAVTN RSIKTNPDHN
1460 1470 1480 1490 1500
VVNSDNPLKR PSKETPTSEN KRSKGHETMV DVLVSEEQAV SPSSDVICTN
1510 1520 1530 1540 1550
IKSIANEESS LALRNSIKVE TNCNENSLNV TLDLDQQTIT KEDGKGQVEH
1560 1570 1580 1590 1600
VQRQENQESM NKINSKSFTQ DNIAQYKSVK KARPNNEGEN NDYACNVEQA
1610 1620 1630 1640 1650
SPVRNEVPGD GIQIPSGTIL LNSSKQTEKS KVDDLRSDED EHGTVAQEKH
1660 1670 1680 1690 1700
QVGAINSRNK NNDRMDSTPI QGTEEESREV VMTEEGINVR LEDSGTCELN
1710 1720 1730 1740 1750
KNLKGPLKGD KDANINDDFV PVEENVRDEG FLKSMEHAVS KETGLEEQPE
1760 1770 1780 1790 1800
VADISVLPEI RIPIFNSLKM QGSKSQIKEK LKKRLQRNEL MPPDSPPRMT
1810 1820 1830 1840 1850
ENTNINAQNG LDTVPKTIGG KEKHHEIQLG QAHTEADGEP LLGGDGNEDA
1860 1870 1880 1890 1900
TSREATPSLK VHFFSKKSRR LVARLRGFTP GDLNGISVEE RRNLRIELLD
1910
FMMRLEYYSN RDNDMN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 580 | C → S in CAA47121 (PubMed:1577274).Curated | 1 | |
Sequence conflicti | 732 | A → T in CAA85238 (PubMed:7813418).Curated | 1 | |
Sequence conflicti | 732 | A → T in CAA53638 (PubMed:8091861).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66501 Genomic DNA Translation: CAA47121.1 Z36144 Genomic DNA Translation: CAA85238.1 Z36145 Genomic DNA Translation: CAA85240.1 X76053 Genomic DNA Translation: CAA53638.1 BK006936 Genomic DNA Translation: DAA07391.2 |
PIRi | S46157 |
RefSeqi | NP_009834.4, NM_001178623.4 |
Genome annotation databases
EnsemblFungii | YBR275C_mRNA; YBR275C; YBR275C |
GeneIDi | 852578 |
KEGGi | sce:YBR275C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66501 Genomic DNA Translation: CAA47121.1 Z36144 Genomic DNA Translation: CAA85238.1 Z36145 Genomic DNA Translation: CAA85240.1 X76053 Genomic DNA Translation: CAA53638.1 BK006936 Genomic DNA Translation: DAA07391.2 |
PIRi | S46157 |
RefSeqi | NP_009834.4, NM_001178623.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4BJS | X-ray | 1.94 | A/B/C/D | 1857-1916 | [»] | |
4BJT | X-ray | 1.61 | D/E/F | 1752-1771 | [»] | |
5NVR | X-ray | 3.95 | A | 177-1282 | [»] | |
5NW5 | X-ray | 6.50 | A/B | 100-1321 | [»] | |
AlphaFoldDBi | P29539 | |||||
SMRi | P29539 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32970, 127 interactors |
ComplexPortali | CPX-2112, Telosome |
DIPi | DIP-801N |
IntActi | P29539, 8 interactors |
MINTi | P29539 |
STRINGi | 4932.YBR275C |
PTM databases
iPTMneti | P29539 |
SwissPalmi | P29539 |
Proteomic databases
MaxQBi | P29539 |
PaxDbi | P29539 |
PRIDEi | P29539 |
Genome annotation databases
EnsemblFungii | YBR275C_mRNA; YBR275C; YBR275C |
GeneIDi | 852578 |
KEGGi | sce:YBR275C |
Organism-specific databases
SGDi | S000000479, RIF1 |
VEuPathDBi | FungiDB:YBR275C |
Phylogenomic databases
eggNOGi | ENOG502QPT7, Eukaryota |
HOGENOMi | CLU_002800_0_0_1 |
InParanoidi | P29539 |
OMAi | CDARRIN |
Miscellaneous databases
PROi | PR:P29539 |
RNActi | P29539, protein |
Family and domain databases
DisProti | DP01607 |
IDEALi | IID50206 |
InterProi | View protein in InterPro IPR028566, Rif1 IPR022031, Rif1_N |
PANTHERi | PTHR22928, PTHR22928, 1 hit |
Pfami | View protein in Pfam PF12231, Rif1_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RIF1_YEAST | |
Accessioni | P29539Primary (citable) accession number: P29539 Secondary accession number(s): D6VQS1, P89507 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 173 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families