UniProtKB - P29533 (VCAM1_MOUSE)
Protein
Vascular cell adhesion protein 1
Gene
Vcam1
Organism
Mus musculus (Mouse)
Status
Functioni
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
GO - Molecular functioni
- cell adhesion molecule binding Source: MGI
- integrin binding Source: MGI
- primary amine oxidase activity Source: MGI
GO - Biological processi
- amine metabolic process Source: MGI
- calcium-mediated signaling using intracellular calcium source Source: MGI
- cardiac neuron differentiation Source: MGI
- cell adhesion Source: MGI
- cell-cell adhesion in response to extracellular stimulus Source: MGI
- cell chemotaxis Source: MGI
- cell-matrix adhesion Source: MGI
- cellular response to amyloid-beta Source: MGI
- cellular response to glucose stimulus Source: MGI
- chorio-allantoic fusion Source: MGI
- embryonic placenta morphogenesis Source: MGI
- heart development Source: MGI
- heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
- heterotypic cell-cell adhesion Source: MGI
- innervation Source: MGI
- leukocyte cell-cell adhesion Source: MGI
- leukocyte tethering or rolling Source: MGI
- positive regulation of T cell proliferation Source: MGI
- viral process Source: UniProtKB-KW
- visceral serous pericardium development Source: DFLAT
Keywordsi
Biological process | Cell adhesion, Host-virus interaction |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-216083, Integrin cell surface interactions |
Names & Taxonomyi
Protein namesi | Recommended name: Vascular cell adhesion protein 1Short name: V-CAM 1 Short name: VCAM-1 Alternative name(s): CD_antigen: CD106 |
Gene namesi | Name:Vcam1 Synonyms:Vcam-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98926, Vcam1 |
Subcellular locationi
Plasma membrane
Plasma membrane
Cytoskeleton
- podosome Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Endosome
- early endosome Source: MGI
Extracellular region or secreted
- extracellular space Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Plasma Membrane
- alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex Source: MGI
- external side of plasma membrane Source: MGI
- integral component of plasma membrane Source: GO_Central
- sarcolemma Source: MGI
Other locations
- anchored component of membrane Source: UniProtKB-KW
- apical part of cell Source: MGI
- cell periphery Source: MGI
- cell surface Source: MGI
- filopodium Source: MGI
- microvillus Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 25 – 698 | ExtracellularSequence analysisAdd BLAST | 674 | |
Transmembranei | 699 – 720 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 721 – 739 | CytoplasmicSequence analysisAdd BLAST | 19 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | CuratedAdd BLAST | 24 | |
ChainiPRO_0000014998 | 25 – 739 | Vascular cell adhesion protein 1Add BLAST | 715 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Disulfide bondi | 47 ↔ 95 | PROSITE-ProRule annotation | |||
Disulfide bondi | 52 ↔ 99 | PROSITE-ProRule annotation | |||
Disulfide bondi | 137 ↔ 195 | PROSITE-ProRule annotation | |||
Glycosylationi | 225 | N-linked (GlcNAc...) asparagine1 Publication | 1 | ||
Disulfide bondi | 246 ↔ 291 | PROSITE-ProRule annotation | |||
Glycosylationi | 273 | N-linked (GlcNAc...) asparagine1 Publication | 1 | ||
Disulfide bondi | 335 ↔ 383 | PROSITE-ProRule annotation | |||
Glycosylationi | 424 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Glycosylationi | 531 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Disulfide bondi | 534 ↔ 579 | PROSITE-ProRule annotation | |||
Glycosylationi | 561 | N-linked (GlcNAc...) asparagine1 Publication | 1 | ||
Isoform 2 (identifier: P29533-2) | |||||
Lipidationi | 319 | GPI-anchor amidated asparagine1 Publication | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
CPTACi | non-CPTAC-3397 |
MaxQBi | P29533 |
PaxDbi | P29533 |
PeptideAtlasi | P29533 |
PRIDEi | P29533 |
PTM databases
GlyConnecti | 2815, 6 N-Linked glycans (2 sites) |
GlyGeni | P29533, 5 sites |
iPTMneti | P29533 |
PhosphoSitePlusi | P29533 |
Expressioni
Tissue specificityi
Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027962, Expressed in spleen and 297 other tissues |
Interactioni
Subunit structurei
(Microbial infection) Interacts with ECMV-D capsid proteins and acts as a receptor for this virus.
1 PublicationGO - Molecular functioni
- cell adhesion molecule binding Source: MGI
- integrin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204505, 3 interactors |
DIPi | DIP-29097N |
IntActi | P29533, 2 interactors |
MINTi | P29533 |
STRINGi | 10090.ENSMUSP00000029574 |
Miscellaneous databases
RNActi | P29533, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 111 | Ig-like C2-type 1Add BLAST | 87 | |
Domaini | 119 – 212 | Ig-like C2-type 2Add BLAST | 94 | |
Domaini | 223 – 309 | Ig-like C2-type 3Add BLAST | 87 | |
Domaini | 312 – 393 | Ig-like C2-type 4Add BLAST | 82 | |
Domaini | 408 – 506 | Ig-like C2-type 5Add BLAST | 99 | |
Domaini | 511 – 595 | Ig-like C2-type 6Add BLAST | 85 | |
Domaini | 600 – 682 | Ig-like C2-type 7Add BLAST | 83 |
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QSKQ, Eukaryota |
GeneTreei | ENSGT00940000156511 |
InParanoidi | P29533 |
PhylomeDBi | P29533 |
Family and domain databases
Gene3Di | 2.60.40.10, 7 hits |
InterProi | View protein in InterPro IPR003987, ICAM_VCAM_N IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR008424, Ig_C2-set IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013151, Immunoglobulin IPR003989, VCAM-1 |
PANTHERi | PTHR13771:SF14, PTHR13771:SF14, 5 hits |
Pfami | View protein in Pfam PF05790, C2-set, 2 hits PF07679, I-set, 3 hits PF00047, ig, 1 hit |
PRINTSi | PR01472, ICAMVCAM1 PR01474, VCAM1 |
SMARTi | View protein in SMART SM00409, IG, 5 hits SM00408, IGc2, 5 hits |
SUPFAMi | SSF48726, SSF48726, 7 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 5 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P29533-1) [UniParc]FASTAAdd to basket
Also known as: Long
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPVKMVAVLG ASTVLWILFA VSQAFKIEIS PEYKTIAQIG DSMALTCSTT
60 70 80 90 100
GCESPLFSWR TQIDSPLNAK VRTEGSKSVL TMEPVSFENE HSYLCTATCG
110 120 130 140 150
SGKLERSIHV DIYSFPKDPE IQFSGPLEVG KPVTVKCLAP DIYPVYRLEI
160 170 180 190 200
DLFKGDQLMN RQEFSSEEMT KSLETKSLEV TFTPVIEDIG KALVCRAKLH
210 220 230 240 250
IDQIDSTLKE RETVKELQVY ISPRNTTISV HPSTRLQEGG AVTMTCSSEG
260 270 280 290 300
LPAPEIFWGR KLDNEVLQLL SGNATLTLIA MRMEDSGVYV CEGVNLIGRD
310 320 330 340 350
KAEVELVVQE KPFIVDISPG SQVAAQVGDS VVLTCAAIGC DSPSFSWRTQ
360 370 380 390 400
TDSPLNGVVR NEGAKSTLVL SSVGFEDEHS YLCAVTCLQR TLEKRTQVEV
410 420 430 440 450
YSFPEDPVIK MSGPLVHGRP VTVNCTVPNV YPFDHLEIEL LKGETTLMKK
460 470 480 490 500
YFLEEMGIKS LETKILETTF IPTIEDTGKS LVCLARLHSG EMESEPKQRQ
510 520 530 540 550
SVQPLYVNVA PKETTIWVSP SPILEEGSPV NLTCSSDGIP APKILWSRQL
560 570 580 590 600
NNGELQPLSE NTTLTFMSTK RDDSGIYVCE GINEAGISRK SVELIIQVSP
610 620 630 640 650
KDIQLTVFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAK TGDMVLKSVD
660 670 680 690 700
GSYTIRQAQL QDAGIYECES KTEVGSQLRS LTLDVKGKEH NKDYFSPELL
710 720 730
ALYCASSLVI PAIGMIVYFA RKANMKGSYS LVEAQKSKV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3UPN1 | Q3UPN1_MOUSE | Vascular cell adhesion protein 1 | Vcam1 | 739 | Annotation score: | ||
A0A0G2JFP9 | A0A0G2JFP9_MOUSE | Vascular cell adhesion protein 1 | Vcam1 | 237 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 693 | D → N in AAA16921 (PubMed:7507076).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002581 | 310 – 345 | EKPFI…DSPSF → DGRMKSQITNGHQLTVHLMF AKSFYFICYLCLYLAL in isoform 2. 3 PublicationsAdd BLAST | 36 | |
Alternative sequenceiVSP_002582 | 346 – 739 | Missing in isoform 2. 3 PublicationsAdd BLAST | 394 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84487 mRNA Translation: AAA40545.1 X67783 mRNA Translation: CAA47989.1 L22355 , L22301, L22349, L22350, L22351, L22352, L22353, L22354 Genomic DNA Translation: AAA16921.1 L22350, L22301, L22349 Genomic DNA Translation: AAA16920.1 U12878 Genomic DNA Translation: AAB60659.1 Sequence problems. U12879 Genomic DNA Translation: AAB60660.1 Sequence problems. U12880 Genomic DNA Translation: AAB60661.1 Sequence problems. U12874 Genomic DNA Translation: AAB60662.1 Sequence problems. U12871 Genomic DNA Translation: AAB60663.1 Sequence problems. U12883 Genomic DNA Translation: AAB60664.1 Sequence problems. U12881 Genomic DNA Translation: AAA80010.1 Sequence problems. U12882 Genomic DNA Translation: AAA80011.1 Sequence problems. U12875 Genomic DNA Translation: AAA80012.1 Sequence problems. U12872 Genomic DNA Translation: AAA80013.1 Sequence problems. U12876 Genomic DNA Translation: AAA80014.1 Sequence problems. U12873 Genomic DNA Translation: AAA80015.1 Sequence problems. U12877 Genomic DNA Translation: AAA80016.1 Sequence problems. L08431 mRNA Translation: AAA40546.1 U12884 mRNA Translation: AAA64832.1 L12541 mRNA Translation: AAC37607.1 U42327 Genomic DNA Translation: AAB88576.1 |
CCDSi | CCDS17785.1 [P29533-1] |
PIRi | B48919, A46052 JN0581 |
Genome annotation databases
Ensembli | ENSMUST00000196449; ENSMUSP00000142876; ENSMUSG00000027962 [P29533-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding VCAM-1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84487 mRNA Translation: AAA40545.1 X67783 mRNA Translation: CAA47989.1 L22355 , L22301, L22349, L22350, L22351, L22352, L22353, L22354 Genomic DNA Translation: AAA16921.1 L22350, L22301, L22349 Genomic DNA Translation: AAA16920.1 U12878 Genomic DNA Translation: AAB60659.1 Sequence problems. U12879 Genomic DNA Translation: AAB60660.1 Sequence problems. U12880 Genomic DNA Translation: AAB60661.1 Sequence problems. U12874 Genomic DNA Translation: AAB60662.1 Sequence problems. U12871 Genomic DNA Translation: AAB60663.1 Sequence problems. U12883 Genomic DNA Translation: AAB60664.1 Sequence problems. U12881 Genomic DNA Translation: AAA80010.1 Sequence problems. U12882 Genomic DNA Translation: AAA80011.1 Sequence problems. U12875 Genomic DNA Translation: AAA80012.1 Sequence problems. U12872 Genomic DNA Translation: AAA80013.1 Sequence problems. U12876 Genomic DNA Translation: AAA80014.1 Sequence problems. U12873 Genomic DNA Translation: AAA80015.1 Sequence problems. U12877 Genomic DNA Translation: AAA80016.1 Sequence problems. L08431 mRNA Translation: AAA40546.1 U12884 mRNA Translation: AAA64832.1 L12541 mRNA Translation: AAC37607.1 U42327 Genomic DNA Translation: AAB88576.1 |
CCDSi | CCDS17785.1 [P29533-1] |
PIRi | B48919, A46052 JN0581 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 204505, 3 interactors |
DIPi | DIP-29097N |
IntActi | P29533, 2 interactors |
MINTi | P29533 |
STRINGi | 10090.ENSMUSP00000029574 |
PTM databases
GlyConnecti | 2815, 6 N-Linked glycans (2 sites) |
GlyGeni | P29533, 5 sites |
iPTMneti | P29533 |
PhosphoSitePlusi | P29533 |
Proteomic databases
CPTACi | non-CPTAC-3397 |
MaxQBi | P29533 |
PaxDbi | P29533 |
PeptideAtlasi | P29533 |
PRIDEi | P29533 |
Protocols and materials databases
Antibodypediai | 3955, 2073 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000196449; ENSMUSP00000142876; ENSMUSG00000027962 [P29533-2] |
Organism-specific databases
MGIi | MGI:98926, Vcam1 |
Phylogenomic databases
eggNOGi | ENOG502QSKQ, Eukaryota |
GeneTreei | ENSGT00940000156511 |
InParanoidi | P29533 |
PhylomeDBi | P29533 |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-216083, Integrin cell surface interactions |
Miscellaneous databases
ChiTaRSi | Vcam1, mouse |
PROi | PR:P29533 |
RNActi | P29533, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027962, Expressed in spleen and 297 other tissues |
Family and domain databases
Gene3Di | 2.60.40.10, 7 hits |
InterProi | View protein in InterPro IPR003987, ICAM_VCAM_N IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR008424, Ig_C2-set IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013151, Immunoglobulin IPR003989, VCAM-1 |
PANTHERi | PTHR13771:SF14, PTHR13771:SF14, 5 hits |
Pfami | View protein in Pfam PF05790, C2-set, 2 hits PF07679, I-set, 3 hits PF00047, ig, 1 hit |
PRINTSi | PR01472, ICAMVCAM1 PR01474, VCAM1 |
SMARTi | View protein in SMART SM00409, IG, 5 hits SM00408, IGc2, 5 hits |
SUPFAMi | SSF48726, SSF48726, 7 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 5 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VCAM1_MOUSE | |
Accessioni | P29533Primary (citable) accession number: P29533 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 191 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot