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UniProtKB - P29475 (NOS1_HUMAN)

Protein

Nitric oxide synthase, brain

Gene

NOS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:

Activity regulationi

Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi420Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi886 – 917FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi1032 – 1043FADBy similarityAdd BLAST12
Nucleotide bindingi1175 – 1185FADBy similarityAdd BLAST11
Nucleotide bindingi1250 – 1268NADPBy similarityAdd BLAST19
Nucleotide bindingi1348 – 1363NADPBy similarityAdd BLAST16

GO - Molecular functioni

  • arginine binding Source: BHF-UCL
  • cadmium ion binding Source: BHF-UCL
  • calmodulin binding Source: UniProtKB-KW
  • flavin adenine dinucleotide binding Source: BHF-UCL
  • FMN binding Source: BHF-UCL
  • heme binding Source: BHF-UCL
  • ion channel binding Source: BHF-UCL
  • NADP binding Source: BHF-UCL
  • NADPH-hemoprotein reductase activity Source: GO_Central
  • nitric-oxide synthase activity Source: CACAO
  • oxidoreductase activity Source: GO_Central
  • scaffold protein binding Source: BHF-UCL
  • sodium channel regulator activity Source: BHF-UCL
  • tetrahydrobiopterin binding Source: BHF-UCL
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:HS01647-MONOMER
BRENDAi1.14.13.39 2681
ReactomeiR-HSA-1222556 ROS, RNS production in phagocytes
R-HSA-392154 Nitric oxide stimulates guanylate cyclase
R-HSA-5578775 Ion homeostasis
SIGNORiP29475

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, brain (EC:1.14.13.39)
Alternative name(s):
Constitutive NOS
NC-NOS
NOS type I
Neuronal NOS
Short name:
N-NOS
Short name:
nNOS
Peptidyl-cysteine S-nitrosylase NOS1
bNOS
Gene namesi
Name:NOS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000089250.18
HGNCiHGNC:7872 NOS1
MIMi163731 gene
neXtProtiNX_P29475

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4842
MalaCardsiNOS1
OpenTargetsiENSG00000089250
Orphaneti930 Idiopathic achalasia
PharmGKBiPA252

Chemistry databases

ChEMBLiCHEMBL3568
DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose
DB01997 3-Bromo-7-Nitroindazole
DB03892 5-N-Allyl-Arginine
DB00997 Doxorubicin
DB03147 Flavin adenine dinucleotide
DB01942 Formic Acid
DB00155 L-Citrulline
DB02077 L-N(Omega)-Nitroarginine-(4r)-Amino-L-Proline Amide
DB09241 Methylene blue
DB02044 N-(3-(Aminomethyl)Benzyl)Acetamidine
DB03449 N-(4-(2-((3-Chlorophenylmethyl)Amino)Ethyl)Phenyl)-2-Thiophecarboxamidine
DB02727 N-Butyl-N'-Hydroxyguanidine
DB02143 N-Isopropyl-N'-Hydroxyguanidine
DB03144 N-Omega-Hydroxy-L-Arginine
DB02644 N-Omega-Propyl-L-Arginine
DB02027 N-{(4s)-4-Amino-5-[(2-Aminoethyl)Amino]Pentyl}-N'-Nitroguanidine
DB03710 N5-(1-Imino-3-Butenyl)-L-Ornithine
DB04223 Nitroarginine
DB06096 NXN-188
DB03247 Riboflavin Monophosphate
DB02991 S-Ethyl-N-[4-(Trifluoromethyl)Phenyl]Isothiourea
DB03707 S-Ethyl-N-Phenyl-Isothiourea
GuidetoPHARMACOLOGYi1251

Polymorphism and mutation databases

BioMutaiNOS1
DMDMi1709333

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001709211 – 1434Nitric oxide synthase, brainAdd BLAST1434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei852PhosphoserineBy similarity1
Modified residuei862PhosphoserineBy similarity1
Modified residuei863PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP29475
MaxQBiP29475
PaxDbiP29475
PeptideAtlasiP29475
PRIDEiP29475
ProteomicsDBi54578
54579 [P29475-2]
54580 [P29475-3]
54581 [P29475-4]

PTM databases

iPTMnetiP29475
PhosphoSitePlusiP29475

Miscellaneous databases

PMAP-CutDBiP29475

Expressioni

Tissue specificityi

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Gene expression databases

BgeeiENSG00000089250 Expressed in 115 organ(s), highest expression level in vastus lateralis
CleanExiHS_NOS1
ExpressionAtlasiP29475 baseline and differential
GenevisibleiP29475 HS

Organism-specific databases

HPAiCAB002167
HPA058312

Interactioni

Subunit structurei

Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with HTR4. Interacts with VAC14 (By similarity). Interacts with SLC6A4 (By similarity). Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
VAC14Q08AM65EBI-7164065,EBI-2107455

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi110905, 15 interactors
CORUMiP29475
DIPiDIP-40999N
IntActiP29475, 4 interactors
MINTiP29475
STRINGi9606.ENSP00000337459

Chemistry databases

BindingDBiP29475

Structurei

Secondary structure

11434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP29475
SMRiP29475
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 99PDZPROSITE-ProRule annotationAdd BLAST83
Domaini760 – 940Flavodoxin-likePROSITE-ProRule annotationAdd BLAST181
Domaini995 – 1242FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST248

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 205Interaction with NOSIPBy similarityAdd BLAST205
Regioni163 – 245PIN (nNOS-inhibiting protein) bindingAdd BLAST83
Regioni730 – 750Calmodulin-bindingSequence analysisAdd BLAST21
Regioni755 – 774Tetrahydrobiopterin-bindingBy similarityAdd BLAST20

Domaini

The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14 (By similarity).By similarity

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
COG0369 LUCA
COG4362 LUCA
GeneTreeiENSGT00840000129757
HOGENOMiHOG000220884
HOVERGENiHBG000159
InParanoidiP29475
KOiK13240
OMAiHNRSRET
OrthoDBiEOG091G10Z0
PhylomeDBiP29475
TreeFamiTF324410

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR003097 CysJ-like_FAD-binding
IPR017927 FAD-bd_FR_type
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR012144 NOS_euk
IPR004030 NOS_N
IPR036119 NOS_N_sf
IPR001433 OxRdtase_FAD/NAD-bd
IPR001478 PDZ
IPR036034 PDZ_sf
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PF02898 NO_synthase, 1 hit
PF00595 PDZ, 1 hit
PIRSFiPIRSF000333 NOS, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF56512 SSF56512, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit
PS60001 NOS, 1 hit
PS50106 PDZ, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Isoform 3 is produced by different alternative splicing events implicating either the untranslated exons TEX1 (TN-NOS) or TEX1B (TN-NOSB) leading to a N-terminally truncated protein which possesses enzymatic activity comparable to that of isoform 1. The C-terminally truncated isoform 4 is produced by insertion of the TEX2 exon between exons 3 and 4 of isoform 1, leading to a frameshift and a premature stop codon.1 Publication

This entry has 5 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P29475-1) [UniParc]FASTAAdd to basket
Also known as: N-NOS-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDHMFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG 
        60         70         80         90        100
AAEQSGLIQA GDIILAVNGR PLVDLSYDSA LEVLRGIASE THVVLILRGP 
       110        120        130        140        150
EGFTTHLETT FTGDGTPKTI RVTQPLGPPT KAVDLSHQPP AGKEQPLAVD 
       160        170        180        190        200
GASGPGNGPQ HAYDDGQEAG SLPHANGLAP RPPGQDPAKK ATRVSLQGRG 
       210        220        230        240        250
ENNELLKEIE PVLSLLTSGS RGVKGGAPAK AEMKDMGIQV DRDLDGKSHK 
       260        270        280        290        300
PLPLGVENDR VFNDLWGKGN VPVVLNNPYS EKEQPPTSGK QSPTKNGSPS 
       310        320        330        340        350
KCPRFLKVKN WETEVVLTDT LHLKSTLETG CTEYICMGSI MHPSQHARRP 
       360        370        380        390        400
EDVRTKGQLF PLAKEFIDQY YSSIKRFGSK AHMERLEEVN KEIDTTSTYQ 
       410        420        430        440        450
LKDTELIYGA KHAWRNASRC VGRIQWSKLQ VFDARDCTTA HGMFNYICNH 
       460        470        480        490        500
VKYATNKGNL RSAITIFPQR TDGKHDFRVW NSQLIRYAGY KQPDGSTLGD 
       510        520        530        540        550
PANVQFTEIC IQQGWKPPRG RFDVLPLLLQ ANGNDPELFQ IPPELVLEVP 
       560        570        580        590        600
IRHPKFEWFK DLGLKWYGLP AVSNMLLEIG GLEFSACPFS GWYMGTEIGV 
       610        620        630        640        650
RDYCDNSRYN ILEEVAKKMN LDMRKTSSLW KDQALVEINI AVLYSFQSDK 
       660        670        680        690        700
VTIVDHHSAT ESFIKHMENE YRCRGGCPAD WVWIVPPMSG SITPVFHQEM 
       710        720        730        740        750
LNYRLTPSFE YQPDPWNTHV WKGTNGTPTK RRAIGFKKLA EAVKFSAKLM 
       760        770        780        790        800
GQAMAKRVKA TILYATETGK SQAYAKTLCE IFKHAFDAKV MSMEEYDIVH 
       810        820        830        840        850
LEHETLVLVV TSTFGNGDPP ENGEKFGCAL MEMRHPNSVQ EERKSYKVRF 
       860        870        880        890        900
NSVSSYSDSQ KSSGDGPDLR DNFESAGPLA NVRFSVFGLG SRAYPHFCAF 
       910        920        930        940        950
GHAVDTLLEE LGGERILKMR EGDELCGQEE AFRTWAKKVF KAACDVFCVG 
       960        970        980        990       1000
DDVNIEKANN SLISNDRSWK RNKFRLTFVA EAPELTQGLS NVHKKRVSAA 
      1010       1020       1030       1040       1050
RLLSRQNLQS PKSSRSTIFV RLHTNGSQEL QYQPGDHLGV FPGNHEDLVN 
      1060       1070       1080       1090       1100
ALIERLEDAP PVNQMVKVEL LEERNTALGV ISNWTDELRL PPCTIFQAFK 
      1110       1120       1130       1140       1150
YYLDITTPPT PLQLQQFASL ATSEKEKQRL LVLSKGLQEY EEWKWGKNPT 
      1160       1170       1180       1190       1200
IVEVLEEFPS IQMPATLLLT QLSLLQPRYY SISSSPDMYP DEVHLTVAIV 
      1210       1220       1230       1240       1250
SYRTRDGEGP IHHGVCSSWL NRIQADELVP CFVRGAPSFH LPRNPQVPCI 
      1260       1270       1280       1290       1300
LVGPGTGIAP FRSFWQQRQF DIQHKGMNPC PMVLVFGCRQ SKIDHIYREE 
      1310       1320       1330       1340       1350
TLQAKNKGVF RELYTAYSRE PDKPKKYVQD ILQEQLAESV YRALKEQGGH 
      1360       1370       1380       1390       1400
IYVCGDVTMA ADVLKAIQRI MTQQGKLSAE DAGVFISRMR DDNRYHEDIF 
      1410       1420       1430 
GVTLRTYEVT NRLRSESIAF IEESKKDTDE VFSS
Length:1,434
Mass (Da):160,970
Last modified:October 1, 1996 - v2
Checksum:i99235793B953BF37
GO
Isoform 2 (identifier: P29475-2) [UniParc]FASTAAdd to basket
Also known as: N-NOS-2

The sequence of this isoform differs from the canonical sequence as follows:
     509-613: Missing.

Show »
Length:1,329
Mass (Da):148,919
Checksum:iDF791B80FDB12302
GO
Isoform 3 (identifier: P29475-3) [UniParc]FASTAAdd to basket
Also known as: TN-NOS, TN-NOSB

The sequence of this isoform differs from the canonical sequence as follows:
     1-336: Missing.

Show »
Length:1,098
Mass (Da):125,113
Checksum:iA1CD5C5012436233
GO
Isoform 4 (identifier: P29475-4) [UniParc]FASTAAdd to basket
Also known as: TEX2-insertion

The sequence of this isoform differs from the canonical sequence as follows:
     285-407: PPTSGKQSPT...TYQLKDTELI → MRKLRITEGF...PKPTWKGWKR
     408-1434: Missing.

Show »
Length:407
Mass (Da):43,838
Checksum:i7E9420C658EFACD2
GO
Isoform 5 (identifier: P29475-5) [UniParc]FASTAAdd to basket
Also known as: nNOSmu

The sequence of this isoform differs from the canonical sequence as follows:
     844-844: K → KYPEPLRFFPRKGPPLPNGDTEVHGLAAARDSQHR

Show »
Length:1,468
Mass (Da):164,779
Checksum:i95906AC90699A0E2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J5P6C9J5P6_HUMAN
Nitric oxide synthase
NOS1
1,433Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131K → E in AAB49040 (PubMed:8879752).Curated1
Sequence conflicti178 – 184LAPRPPG → WPQAPR (PubMed:7678401).Curated7
Sequence conflicti178 – 184LAPRPPG → WPQAPR (PubMed:8879752).Curated7
Sequence conflicti492 – 493QP → HR in AAA36376 (PubMed:7678401).Curated2
Sequence conflicti549V → L in AAA36376 (PubMed:7678401).Curated1
Sequence conflicti563G → A in AAA36376 (PubMed:7678401).Curated1
Sequence conflicti1407Y → I in AAA36376 (PubMed:7678401).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018948228P → S1 PublicationCorresponds to variant dbSNP:rs9658279Ensembl.1
Natural variantiVAR_018949394D → A1 PublicationCorresponds to variant dbSNP:rs9658356Ensembl.1
Natural variantiVAR_018950725N → D1 PublicationCorresponds to variant dbSNP:rs9658403Ensembl.1
Natural variantiVAR_018951864G → D1 PublicationCorresponds to variant dbSNP:rs9658445Ensembl.1
Natural variantiVAR_0189521064Q → R1 PublicationCorresponds to variant dbSNP:rs9658482Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0035711 – 336Missing in isoform 3. CuratedAdd BLAST336
Alternative sequenceiVSP_003572285 – 407PPTSG…DTELI → MRKLRITEGFGVQRGSHNHP PPQENSPPQRMAAPPSVHAS SRSRTGRLRWFSLTPSTLRA HWKRDALSTSAWAPSCILLS MQGGLKTSAQKDSSSLSPKS LLINTIHQLKDLAPKPTWKG WKR in isoform 4. CuratedAdd BLAST123
Alternative sequenceiVSP_003573408 – 1434Missing in isoform 4. CuratedAdd BLAST1027
Alternative sequenceiVSP_003574509 – 613Missing in isoform 2. 2 PublicationsAdd BLAST105
Alternative sequenceiVSP_044916844K → KYPEPLRFFPRKGPPLPNGD TEVHGLAAARDSQHR in isoform 5. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17327 mRNA Translation: AAA62405.1
U17326
, U17299, U17300, U17301, U17302, U17303, U17304, U17305, U17307, U17308, U17309, U17310, U17311, U17312, U17313, U17314, U17315, U17316, U17317, U17318, U17319, U17320, U17321, U17322, U17323, U17324, U17325 Genomic DNA Translation: AAB60654.1 Sequence problems.
D16408 mRNA Translation: BAA03895.1
L02881 mRNA Translation: AAA36376.1
U31466 mRNA Translation: AAB49040.1
U66362 Genomic DNA No translation available.
AY445095 Genomic DNA Translation: AAR07069.1
AC026364 Genomic DNA No translation available.
AC068799 Genomic DNA No translation available.
AC073864 Genomic DNA No translation available.
AJ004918 mRNA Translation: CAA06218.1
CCDSiCCDS41842.1 [P29475-1]
CCDS55890.1 [P29475-5]
PIRiG01946
RefSeqiNP_000611.1, NM_000620.4 [P29475-1]
NP_001191142.1, NM_001204213.1 [P29475-3]
NP_001191143.1, NM_001204214.1 [P29475-3]
NP_001191147.1, NM_001204218.1 [P29475-5]
XP_011536700.1, XM_011538398.2
XP_016874834.1, XM_017019345.1
XP_016874835.1, XM_017019346.1
XP_016874836.1, XM_017019347.1
UniGeneiHs.654410
Hs.684465
Hs.684466
Hs.684467
Hs.735734

Genome annotation databases

EnsembliENST00000317775; ENSP00000320758; ENSG00000089250 [P29475-1]
ENST00000338101; ENSP00000337459; ENSG00000089250 [P29475-5]
ENST00000618760; ENSP00000477999; ENSG00000089250 [P29475-5]
GeneIDi4842
KEGGihsa:4842
UCSCiuc001twm.3 human [P29475-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs
Wikipedia

Nitric oxide synthase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17327 mRNA Translation: AAA62405.1
U17326
, U17299, U17300, U17301, U17302, U17303, U17304, U17305, U17307, U17308, U17309, U17310, U17311, U17312, U17313, U17314, U17315, U17316, U17317, U17318, U17319, U17320, U17321, U17322, U17323, U17324, U17325 Genomic DNA Translation: AAB60654.1 Sequence problems.
D16408 mRNA Translation: BAA03895.1
L02881 mRNA Translation: AAA36376.1
U31466 mRNA Translation: AAB49040.1
U66362 Genomic DNA No translation available.
AY445095 Genomic DNA Translation: AAR07069.1
AC026364 Genomic DNA No translation available.
AC068799 Genomic DNA No translation available.
AC073864 Genomic DNA No translation available.
AJ004918 mRNA Translation: CAA06218.1
CCDSiCCDS41842.1 [P29475-1]
CCDS55890.1 [P29475-5]
PIRiG01946
RefSeqiNP_000611.1, NM_000620.4 [P29475-1]
NP_001191142.1, NM_001204213.1 [P29475-3]
NP_001191143.1, NM_001204214.1 [P29475-3]
NP_001191147.1, NM_001204218.1 [P29475-5]
XP_011536700.1, XM_011538398.2
XP_016874834.1, XM_017019345.1
XP_016874835.1, XM_017019346.1
XP_016874836.1, XM_017019347.1
UniGeneiHs.654410
Hs.684465
Hs.684466
Hs.684467
Hs.735734

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4D1NX-ray2.03A/B/C/D302-721[»]
4UCHX-ray2.20A/B302-723[»]
4UH5X-ray1.98A/B302-722[»]
4UH6X-ray1.98A/B302-722[»]
4V3UX-ray2.30A/B/C/D302-721[»]
5ADFX-ray1.97A/B302-722[»]
5ADGX-ray1.98A/B302-722[»]
5ADIX-ray2.20A/B302-722[»]
5FVUX-ray2.22A/B302-722[»]
5FVVX-ray2.05A/B302-722[»]
5FVWX-ray2.20A/B302-722[»]
5FVXX-ray2.30A/B302-722[»]
5UO1X-ray1.90A/B302-722[»]
5UO2X-ray1.95A/B302-722[»]
5UO3X-ray2.20A/B302-722[»]
5UO4X-ray2.00A/B302-722[»]
5UO5X-ray2.00A/B302-722[»]
5UO6X-ray1.96A/B302-722[»]
5UO7X-ray2.06A/B302-722[»]
5VUVX-ray1.98A/B302-722[»]
5VUWX-ray2.03A/B302-722[»]
5VUXX-ray2.30A/B302-722[»]
5VUYX-ray2.15A/B302-722[»]
5VUZX-ray1.97A/B302-722[»]
5VV0X-ray1.80A/B302-722[»]
5VV1X-ray1.95A/B302-722[»]
5VV2X-ray2.00A/B302-722[»]
5VV3X-ray2.18A/B302-722[»]
5VV4X-ray2.10A/B302-722[»]
5VV5X-ray2.15A/B302-722[»]
6AUYX-ray1.92A/B302-722[»]
6AUZX-ray2.00A/B302-722[»]
6AV0X-ray2.00A/B302-722[»]
6AV1X-ray2.45A/B302-722[»]
6AV2X-ray2.10A/B302-722[»]
6AV3X-ray1.95A/B302-722[»]
6AV4X-ray1.87A/B302-722[»]
6AV5X-ray1.90A/B302-722[»]
ProteinModelPortaliP29475
SMRiP29475
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110905, 15 interactors
CORUMiP29475
DIPiDIP-40999N
IntActiP29475, 4 interactors
MINTiP29475
STRINGi9606.ENSP00000337459

Chemistry databases

BindingDBiP29475
ChEMBLiCHEMBL3568
DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose
DB01997 3-Bromo-7-Nitroindazole
DB03892 5-N-Allyl-Arginine
DB00997 Doxorubicin
DB03147 Flavin adenine dinucleotide
DB01942 Formic Acid
DB00155 L-Citrulline
DB02077 L-N(Omega)-Nitroarginine-(4r)-Amino-L-Proline Amide
DB09241 Methylene blue
DB02044 N-(3-(Aminomethyl)Benzyl)Acetamidine
DB03449 N-(4-(2-((3-Chlorophenylmethyl)Amino)Ethyl)Phenyl)-2-Thiophecarboxamidine
DB02727 N-Butyl-N'-Hydroxyguanidine
DB02143 N-Isopropyl-N'-Hydroxyguanidine
DB03144 N-Omega-Hydroxy-L-Arginine
DB02644 N-Omega-Propyl-L-Arginine
DB02027 N-{(4s)-4-Amino-5-[(2-Aminoethyl)Amino]Pentyl}-N'-Nitroguanidine
DB03710 N5-(1-Imino-3-Butenyl)-L-Ornithine
DB04223 Nitroarginine
DB06096 NXN-188
DB03247 Riboflavin Monophosphate
DB02991 S-Ethyl-N-[4-(Trifluoromethyl)Phenyl]Isothiourea
DB03707 S-Ethyl-N-Phenyl-Isothiourea
GuidetoPHARMACOLOGYi1251

PTM databases

iPTMnetiP29475
PhosphoSitePlusiP29475

Polymorphism and mutation databases

BioMutaiNOS1
DMDMi1709333

Proteomic databases

EPDiP29475
MaxQBiP29475
PaxDbiP29475
PeptideAtlasiP29475
PRIDEiP29475
ProteomicsDBi54578
54579 [P29475-2]
54580 [P29475-3]
54581 [P29475-4]

Protocols and materials databases

DNASUi4842
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000317775; ENSP00000320758; ENSG00000089250 [P29475-1]
ENST00000338101; ENSP00000337459; ENSG00000089250 [P29475-5]
ENST00000618760; ENSP00000477999; ENSG00000089250 [P29475-5]
GeneIDi4842
KEGGihsa:4842
UCSCiuc001twm.3 human [P29475-1]

Organism-specific databases

CTDi4842
DisGeNETi4842
EuPathDBiHostDB:ENSG00000089250.18
GeneCardsiNOS1
HGNCiHGNC:7872 NOS1
HPAiCAB002167
HPA058312
MalaCardsiNOS1
MIMi163731 gene
neXtProtiNX_P29475
OpenTargetsiENSG00000089250
Orphaneti930 Idiopathic achalasia
PharmGKBiPA252
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
COG0369 LUCA
COG4362 LUCA
GeneTreeiENSGT00840000129757
HOGENOMiHOG000220884
HOVERGENiHBG000159
InParanoidiP29475
KOiK13240
OMAiHNRSRET
OrthoDBiEOG091G10Z0
PhylomeDBiP29475
TreeFamiTF324410

Enzyme and pathway databases

BioCyciMetaCyc:HS01647-MONOMER
BRENDAi1.14.13.39 2681
ReactomeiR-HSA-1222556 ROS, RNS production in phagocytes
R-HSA-392154 Nitric oxide stimulates guanylate cyclase
R-HSA-5578775 Ion homeostasis
SIGNORiP29475

Miscellaneous databases

ChiTaRSiNOS1 human
GeneWikiiNOS1
GenomeRNAii4842
PMAP-CutDBiP29475
PROiPR:P29475
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000089250 Expressed in 115 organ(s), highest expression level in vastus lateralis
CleanExiHS_NOS1
ExpressionAtlasiP29475 baseline and differential
GenevisibleiP29475 HS

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR003097 CysJ-like_FAD-binding
IPR017927 FAD-bd_FR_type
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR012144 NOS_euk
IPR004030 NOS_N
IPR036119 NOS_N_sf
IPR001433 OxRdtase_FAD/NAD-bd
IPR001478 PDZ
IPR036034 PDZ_sf
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PF02898 NO_synthase, 1 hit
PF00595 PDZ, 1 hit
PIRSFiPIRSF000333 NOS, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF56512 SSF56512, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit
PS60001 NOS, 1 hit
PS50106 PDZ, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOS1_HUMAN
AccessioniPrimary (citable) accession number: P29475
Secondary accession number(s): E9PH30, O75713
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 208 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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