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Protein

Interleukin-12 subunit alpha

Gene

IL12A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.3 Publications

GO - Molecular functioni

  • cytokine activity Source: UniProtKB-KW
  • growth factor activity Source: UniProtKB-KW
  • interleukin-12 beta subunit binding Source: AgBase
  • interleukin-12 receptor binding Source: UniProtKB
  • interleukin-27 binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-HSA-6783783 Interleukin-10 signaling
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-8984722 Interleukin-35 Signalling
R-HSA-9020591 Interleukin-12 signaling
SignaLinkiP29459
SIGNORiP29459

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit alpha
Short name:
IL-12A
Alternative name(s):
Cytotoxic lymphocyte maturation factor 35 kDa subunit
Short name:
CLMF p35
IL-12 subunit p35
NK cell stimulatory factor chain 1
Short name:
NKSF1
Gene namesi
Name:IL12A
Synonyms:NKSF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000168811.6
HGNCiHGNC:5969 IL12A
MIMi161560 gene
neXtProtiNX_P29459

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi3592
MalaCardsiIL12A
Orphaneti117 Behcet disease
186 Primary biliary cholangitis
PharmGKBiPA29784

Chemistry databases

ChEMBLiCHEMBL2364153

Polymorphism and mutation databases

BioMutaiIL12A
DMDMi20141534

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000001560423 – 219Interleukin-12 subunit alphaAdd BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi64 ↔ 1961 Publication
Disulfide bondi85 ↔ 1231 Publication
Glycosylationi93N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi96Interchain (with C-199 in IL12B)1 Publication
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP29459
PeptideAtlasiP29459
PRIDEiP29459
ProteomicsDBi54569

PTM databases

iPTMnetiP29459
PhosphoSitePlusiP29459

Expressioni

Inductioni

(Microbial infection) Down-regulated in response to enterovirus 71 (EV71) infection.1 Publication

Gene expression databases

BgeeiENSG00000168811 Expressed in 81 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_IL12A
ExpressionAtlasiP29459 baseline and differential
GenevisibleiP29459 HS

Organism-specific databases

HPAiHPA001886

Interactioni

Subunit structurei

Heterodimer with IL12B; disulfide-linked (PubMed:1674604, PubMed:10899108). This heterodimer is known as interleukin IL-12 (PubMed:1674604). Heterodimer with EBI3/IL27B; not disulfide-linked (PubMed:9342359). This heterodimer is known as interleukin IL-35 (PubMed:9342359).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IL12BP294602EBI-1029636,EBI-1029614

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109806, 7 interactors
ComplexPortaliCPX-381 Interleukin-12 complex
CPX-382 Interleukin-12-receptor complex
CORUMiP29459
DIPiDIP-3772N
IntActiP29459, 3 interactors
STRINGi9606.ENSP00000303231

Structurei

Secondary structure

1219
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP29459
SMRiP29459
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29459

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVIR Eukaryota
ENOG410ZGHJ LUCA
HOVERGENiHBG063691
InParanoidiP29459
KOiK05406
OrthoDBiEOG091G13SK
PhylomeDBiP29459
TreeFamiTF330814

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR004281 IL-12_alpha
PANTHERiPTHR45311 PTHR45311, 1 hit
PfamiView protein in Pfam
PF03039 IL12, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P29459-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCPARSLLLV ATLVLLDHLS LARNLPVATP DPGMFPCLHH SQNLLRAVSN
60 70 80 90 100
MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR
110 120 130 140 150
ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK
160 170 180 190 200
RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH
210
AFRIRAVTID RVMSYLNAS
Length:219
Mass (Da):24,874
Last modified:January 23, 2002 - v2
Checksum:i7C658AB7716112B2
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7ENE1E7ENE1_HUMAN
Interleukin-12 subunit alpha
IL12A
215Annotation score:
E9PGR3E9PGR3_HUMAN
Interleukin-12 subunit alpha
IL12A
239Annotation score:

Sequence cautioni

The sequence AAA59937 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213M → T in AAA35694 (PubMed:1674604).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65291 mRNA Translation: AAA59937.1 Different initiation.
M65271 mRNA Translation: AAA35694.1
AF404773 Genomic DNA Translation: AAK84425.1
AC010370 Genomic DNA No translation available.
RefSeqiNP_000873.2, NM_000882.3
UniGeneiHs.673

Genome annotation databases

EnsembliENST00000305579; ENSP00000303231; ENSG00000168811
GeneIDi3592
KEGGihsa:3592
UCSCiuc003fcx.4 human

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Interleukin-12 entry

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65291 mRNA Translation: AAA59937.1 Different initiation.
M65271 mRNA Translation: AAA35694.1
AF404773 Genomic DNA Translation: AAK84425.1
AC010370 Genomic DNA No translation available.
RefSeqiNP_000873.2, NM_000882.3
UniGeneiHs.673

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F45X-ray2.80B23-219[»]
3HMXX-ray3.00B23-219[»]
ProteinModelPortaliP29459
SMRiP29459
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109806, 7 interactors
ComplexPortaliCPX-381 Interleukin-12 complex
CPX-382 Interleukin-12-receptor complex
CORUMiP29459
DIPiDIP-3772N
IntActiP29459, 3 interactors
STRINGi9606.ENSP00000303231

Chemistry databases

ChEMBLiCHEMBL2364153

PTM databases

iPTMnetiP29459
PhosphoSitePlusiP29459

Polymorphism and mutation databases

BioMutaiIL12A
DMDMi20141534

Proteomic databases

PaxDbiP29459
PeptideAtlasiP29459
PRIDEiP29459
ProteomicsDBi54569

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000305579; ENSP00000303231; ENSG00000168811
GeneIDi3592
KEGGihsa:3592
UCSCiuc003fcx.4 human

Organism-specific databases

CTDi3592
DisGeNETi3592
EuPathDBiHostDB:ENSG00000168811.6
GeneCardsiIL12A
HGNCiHGNC:5969 IL12A
HPAiHPA001886
MalaCardsiIL12A
MIMi161560 gene
neXtProtiNX_P29459
Orphaneti117 Behcet disease
186 Primary biliary cholangitis
PharmGKBiPA29784
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVIR Eukaryota
ENOG410ZGHJ LUCA
HOVERGENiHBG063691
InParanoidiP29459
KOiK05406
OrthoDBiEOG091G13SK
PhylomeDBiP29459
TreeFamiTF330814

Enzyme and pathway databases

ReactomeiR-HSA-6783783 Interleukin-10 signaling
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-8984722 Interleukin-35 Signalling
R-HSA-9020591 Interleukin-12 signaling
SignaLinkiP29459
SIGNORiP29459

Miscellaneous databases

EvolutionaryTraceiP29459
GeneWikiiIL12A
GenomeRNAii3592
PROiPR:P29459
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168811 Expressed in 81 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_IL12A
ExpressionAtlasiP29459 baseline and differential
GenevisibleiP29459 HS

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR004281 IL-12_alpha
PANTHERiPTHR45311 PTHR45311, 1 hit
PfamiView protein in Pfam
PF03039 IL12, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIL12A_HUMAN
AccessioniPrimary (citable) accession number: P29459
Secondary accession number(s): Q96QZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2002
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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