UniProtKB - P29413 (CALR_DROME)
Protein
Calreticulin
Gene
Calr
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | CarbohydrateBy similarity | 1 | |
Binding sitei | 111 | CarbohydrateBy similarity | 1 | |
Binding sitei | 128 | CarbohydrateBy similarity | 1 | |
Binding sitei | 135 | CarbohydrateBy similarity | 1 | |
Binding sitei | 317 | CarbohydrateBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: GO_Central
- carbohydrate binding Source: UniProtKB-KW
- unfolded protein binding Source: InterPro
GO - Biological processi
- endoplasmic reticulum unfolded protein response Source: GO_Central
- olfactory behavior Source: FlyBase
- protein folding Source: GO_Central
- sleep Source: FlyBase
Keywordsi
Molecular function | Chaperone |
Ligand | Calcium, Lectin, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-901042, Calnexin/calreticulin cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Calreticulin1 Publication |
Gene namesi | ORF Names:CG9429Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0005585, Calr |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
- endoplasmic reticulum membrane Source: GO_Central
Extracellular region or secreted
- extracellular space Source: FlyBase
Other locations
- endomembrane system Source: FlyBase
- fusome Source: FlyBase
Keywords - Cellular componenti
Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
ChainiPRO_0000004182 | 18 – 406 | CalreticulinAdd BLAST | 389 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 105 ↔ 137 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P29413 |
PRIDEi | P29413 |
Expressioni
Gene expression databases
Bgeei | FBgn0005585, Expressed in imaginal disc and 65 other tissues |
ExpressionAtlasi | P29413, baseline and differential |
Genevisiblei | P29413, DM |
Interactioni
GO - Molecular functioni
- unfolded protein binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 66325, 93 interactors |
DIPi | DIP-19046N |
IntActi | P29413, 3 interactors |
STRINGi | 7227.FBpp0081581 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 191 – 202 | 1-1Add BLAST | 12 | |
Repeati | 210 – 221 | 1-2Add BLAST | 12 | |
Repeati | 227 – 238 | 1-3Add BLAST | 12 | |
Repeati | 244 – 255 | 1-4Add BLAST | 12 | |
Repeati | 259 – 269 | 2-1Add BLAST | 11 | |
Repeati | 273 – 283 | 2-2Add BLAST | 11 | |
Repeati | 287 – 297 | 2-3Add BLAST | 11 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 191 – 255 | 4 X approximate repeatsAdd BLAST | 65 | |
Regioni | 259 – 297 | 3 X approximate repeatsAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 353 – 402 | Asp/Glu/Lys-richAdd BLAST | 50 |
Domaini
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity
Sequence similaritiesi
Belongs to the calreticulin family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG0674, Eukaryota |
HOGENOMi | CLU_018224_0_2_1 |
InParanoidi | P29413 |
OMAi | VGFELWQ |
OrthoDBi | 822188at2759 |
PhylomeDBi | P29413 |
Family and domain databases
Gene3Di | 2.10.250.10, 2 hits |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009169, Calreticulin IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 2 hits |
PIRSFi | PIRSF002356, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 3 hits PS00014, ER_TARGET, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P29413-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMWCKTVIVL LATVGFISAE VYLKENFDNE NWEDTWIYSK HPGKEFGKFV
60 70 80 90 100
LTPGTFYNDA EADKGIQTSQ DARFYAASRK FDGFSNEDKP LVVQFSVKHE
110 120 130 140 150
QNIDCGGGYV KLFDCSLDQT DMHGESPYEI MFGPDICGPG TKKVHVIFSY
160 170 180 190 200
KGKNHLISKD IRCKDDVYTH FYTLIVRPDN TYEVLIDNEK VESGNLEDDW
210 220 230 240 250
DFLAPKKIKD PTATKPEDWD DRATIPDPDD KKPEDWDKPE HIPDPDATKP
260 270 280 290 300
EDWDDEMDGE WEPPMIDNPE FKGEWQPKQL DNPNYKGAWE HPEIANPEYV
310 320 330 340 350
PDDKLYLRKE ICTLGFDLWQ VKSGTIFDNV LITDDVELAA KAAAEVKNTQ
360 370 380 390 400
AGEKKMKEAQ DEVQRKKDEE EAKKASDKDD EDEDDDDEEK DDESKQDKDQ
SEHDEL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ53YH3 | Q53YH3_DROME | Calreticulin | Calr anon-EST:fe3D7, anon-EST:Liang-2.11, anon-EST:Liang-2.17, chr3R:5454619..5454778, clone 2.11 | 406 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 107 | G → A AA sequence (PubMed:2365822).Curated | 1 | |
Sequence conflicti | 184 | V → L AA sequence (PubMed:2365822).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64461 Genomic DNA Translation: CAA45791.1 AE014297 Genomic DNA Translation: AAF54416.1 |
PIRi | A56637 |
RefSeqi | NP_001262430.1, NM_001275501.1 NP_524293.2, NM_079569.5 |
Genome annotation databases
EnsemblMetazoai | FBtr0082103; FBpp0081581; FBgn0005585 FBtr0334618; FBpp0306685; FBgn0005585 |
GeneIDi | 41166 |
KEGGi | dme:Dmel_CG9429 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64461 Genomic DNA Translation: CAA45791.1 AE014297 Genomic DNA Translation: AAF54416.1 |
PIRi | A56637 |
RefSeqi | NP_001262430.1, NM_001275501.1 NP_524293.2, NM_079569.5 |
3D structure databases
SMRi | P29413 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 66325, 93 interactors |
DIPi | DIP-19046N |
IntActi | P29413, 3 interactors |
STRINGi | 7227.FBpp0081581 |
Proteomic databases
PaxDbi | P29413 |
PRIDEi | P29413 |
Genome annotation databases
EnsemblMetazoai | FBtr0082103; FBpp0081581; FBgn0005585 FBtr0334618; FBpp0306685; FBgn0005585 |
GeneIDi | 41166 |
KEGGi | dme:Dmel_CG9429 |
Organism-specific databases
CTDi | 811 |
FlyBasei | FBgn0005585, Calr |
Phylogenomic databases
eggNOGi | KOG0674, Eukaryota |
HOGENOMi | CLU_018224_0_2_1 |
InParanoidi | P29413 |
OMAi | VGFELWQ |
OrthoDBi | 822188at2759 |
PhylomeDBi | P29413 |
Enzyme and pathway databases
Reactomei | R-DME-901042, Calnexin/calreticulin cycle |
Miscellaneous databases
BioGRID-ORCSi | 41166, 1 hit in 3 CRISPR screens |
ChiTaRSi | Calr, fly |
GenomeRNAii | 41166 |
PROi | PR:P29413 |
Gene expression databases
Bgeei | FBgn0005585, Expressed in imaginal disc and 65 other tissues |
ExpressionAtlasi | P29413, baseline and differential |
Genevisiblei | P29413, DM |
Family and domain databases
Gene3Di | 2.10.250.10, 2 hits |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009169, Calreticulin IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 2 hits |
PIRSFi | PIRSF002356, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 3 hits PS00014, ER_TARGET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CALR_DROME | |
Accessioni | P29413Primary (citable) accession number: P29413 Secondary accession number(s): Q9VHA3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families