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Protein

Guanine nucleotide-binding protein subunit beta-4

Gene

Gnb4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

GO - Biological processi

  • G protein-coupled receptor signaling pathway Source: MGI

Keywordsi

Molecular functionTransducer

Enzyme and pathway databases

ReactomeiR-MMU-163359 Glucagon signaling in metabolic regulation
R-MMU-202040 G-protein activation
R-MMU-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-MMU-392170 ADP signalling through P2Y purinoceptor 12
R-MMU-392451 G beta:gamma signalling through PI3Kgamma
R-MMU-392851 Prostacyclin signalling through prostacyclin receptor
R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion
R-MMU-4086398 Ca2+ pathway
R-MMU-416476 G alpha (q) signalling events
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-418217 G beta:gamma signalling through PLC beta
R-MMU-418555 G alpha (s) signalling events
R-MMU-418592 ADP signalling through P2Y purinoceptor 1
R-MMU-418594 G alpha (i) signalling events
R-MMU-418597 G alpha (z) signalling events
R-MMU-420092 Glucagon-type ligand receptors
R-MMU-428930 Thromboxane signalling through TP receptor
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-MMU-500657 Presynaptic function of Kainate receptors
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-4
Alternative name(s):
Transducin beta chain 4
Gene namesi
Name:Gnb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104581 Gnb4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001277032 – 340Guanine nucleotide-binding protein subunit beta-4Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei266PhosphohistidineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP29387
MaxQBiP29387
PaxDbiP29387
PeptideAtlasiP29387
PRIDEiP29387

2D gel databases

REPRODUCTION-2DPAGEiP29387

PTM databases

iPTMnetiP29387
PhosphoSitePlusiP29387
SwissPalmiP29387

Expressioni

Gene expression databases

BgeeiENSMUSG00000027669 Expressed in 284 organ(s), highest expression level in urinary bladder
ExpressionAtlasiP29387 baseline and differential
GenevisibleiP29387 MM

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma.

Protein-protein interaction databases

BioGridi199980, 1 interactor
DIPiDIP-604N
IntActiP29387, 3 interactors
MINTiP29387
STRINGi10090.ENSMUSP00000103869

Structurei

3D structure databases

ProteinModelPortaliP29387
SMRiP29387
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati53 – 92WD 1Add BLAST40
Repeati95 – 134WD 2Add BLAST40
Repeati141 – 179WD 3Add BLAST39
Repeati182 – 221WD 4Add BLAST40
Repeati224 – 263WD 5Add BLAST40
Repeati268 – 307WD 6Add BLAST40
Repeati310 – 339WD 7Add BLAST30

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286 Eukaryota
ENOG410XQUX LUCA
GeneTreeiENSGT00760000119239
HOGENOMiHOG000176356
HOVERGENiHBG000188
InParanoidiP29387
KOiK04538
OMAiDYKTFVS
OrthoDBiEOG091G0A7T
PhylomeDBiP29387
TreeFamiTF106149

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR016346 Guanine_nucleotide-bd_bsu
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19850 PTHR19850, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 7 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P29387-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSELEQLRQE AEQLRNQIQD ARKACNDATL VQITSNMDSV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGYDSRLL VSASQDGKLI IWDSYTTNKM HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELP GHTGYLSCCR
160 170 180 190 200
FLDDGQIITS SGDTTCALWD IETGQQTTTF TGHSGDVMSL SLSPDLKTFV
210 220 230 240 250
SGACDASSKL WDIRDGMCRQ SFTGHISDIN AVSFFPSGYA FATGSDDATC
260 270 280 290 300
RLFDLRADQE LLLYSHDNII CGITSVAFSK SGRLLLAGYD DFNCSVWDAL
310 320 330 340
KGGRSGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLRIWN
Length:340
Mass (Da):37,379
Last modified:January 23, 2007 - v4
Checksum:iF06827EBC2F0E966
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V9GWY1V9GWY1_MOUSE
Guanine nucleotide-binding protein ...
Gnb4
83Annotation score:
A0A0A6YVN9A0A0A6YVN9_MOUSE
Guanine nucleotide-binding protein ...
Gnb4
73Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132N → D in AAB21609 (PubMed:1543505).Curated1
Sequence conflicti132N → D in AAA37756 (PubMed:1543505).Curated1
Sequence conflicti132N → D in AAA37664 (PubMed:1543505).Curated1
Sequence conflicti140P → A in AAB21609 (PubMed:1543505).Curated1
Sequence conflicti140P → A in AAA37756 (PubMed:1543505).Curated1
Sequence conflicti140P → A in AAA37664 (PubMed:1543505).Curated1
Sequence conflicti238G → V in AAM15922 (PubMed:11842130).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S86124 mRNA Translation: AAB21609.1
M87286 mRNA Translation: AAA37756.1
M63658 mRNA Translation: AAA37664.1
AF277893 mRNA Translation: AAF82124.1
AF501886 mRNA Translation: AAM15922.1
AK036816 mRNA Translation: BAC29589.1
AK152227 mRNA Translation: BAE31054.1
AK167416 mRNA Translation: BAE39504.1
BC028753 mRNA Translation: AAH28753.1
CCDSiCCDS17297.1
PIRiJS0669 RGMSB4
RefSeqiNP_001334991.1, NM_001348062.1
NP_001335033.1, NM_001348104.1
NP_038559.2, NM_013531.5
XP_006535466.1, XM_006535403.3
UniGeneiMm.139192
Mm.393826
Mm.477166

Genome annotation databases

EnsembliENSMUST00000108234; ENSMUSP00000103869; ENSMUSG00000027669
ENSMUST00000155737; ENSMUSP00000121127; ENSMUSG00000027669
GeneIDi14696
KEGGimmu:14696
UCSCiuc008owl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S86124 mRNA Translation: AAB21609.1
M87286 mRNA Translation: AAA37756.1
M63658 mRNA Translation: AAA37664.1
AF277893 mRNA Translation: AAF82124.1
AF501886 mRNA Translation: AAM15922.1
AK036816 mRNA Translation: BAC29589.1
AK152227 mRNA Translation: BAE31054.1
AK167416 mRNA Translation: BAE39504.1
BC028753 mRNA Translation: AAH28753.1
CCDSiCCDS17297.1
PIRiJS0669 RGMSB4
RefSeqiNP_001334991.1, NM_001348062.1
NP_001335033.1, NM_001348104.1
NP_038559.2, NM_013531.5
XP_006535466.1, XM_006535403.3
UniGeneiMm.139192
Mm.393826
Mm.477166

3D structure databases

ProteinModelPortaliP29387
SMRiP29387
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199980, 1 interactor
DIPiDIP-604N
IntActiP29387, 3 interactors
MINTiP29387
STRINGi10090.ENSMUSP00000103869

PTM databases

iPTMnetiP29387
PhosphoSitePlusiP29387
SwissPalmiP29387

2D gel databases

REPRODUCTION-2DPAGEiP29387

Proteomic databases

EPDiP29387
MaxQBiP29387
PaxDbiP29387
PeptideAtlasiP29387
PRIDEiP29387

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108234; ENSMUSP00000103869; ENSMUSG00000027669
ENSMUST00000155737; ENSMUSP00000121127; ENSMUSG00000027669
GeneIDi14696
KEGGimmu:14696
UCSCiuc008owl.1 mouse

Organism-specific databases

CTDi59345
MGIiMGI:104581 Gnb4

Phylogenomic databases

eggNOGiKOG0286 Eukaryota
ENOG410XQUX LUCA
GeneTreeiENSGT00760000119239
HOGENOMiHOG000176356
HOVERGENiHBG000188
InParanoidiP29387
KOiK04538
OMAiDYKTFVS
OrthoDBiEOG091G0A7T
PhylomeDBiP29387
TreeFamiTF106149

Enzyme and pathway databases

ReactomeiR-MMU-163359 Glucagon signaling in metabolic regulation
R-MMU-202040 G-protein activation
R-MMU-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-MMU-392170 ADP signalling through P2Y purinoceptor 12
R-MMU-392451 G beta:gamma signalling through PI3Kgamma
R-MMU-392851 Prostacyclin signalling through prostacyclin receptor
R-MMU-400042 Adrenaline,noradrenaline inhibits insulin secretion
R-MMU-4086398 Ca2+ pathway
R-MMU-416476 G alpha (q) signalling events
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-418217 G beta:gamma signalling through PLC beta
R-MMU-418555 G alpha (s) signalling events
R-MMU-418592 ADP signalling through P2Y purinoceptor 1
R-MMU-418594 G alpha (i) signalling events
R-MMU-418597 G alpha (z) signalling events
R-MMU-420092 Glucagon-type ligand receptors
R-MMU-428930 Thromboxane signalling through TP receptor
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-MMU-500657 Presynaptic function of Kainate receptors
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Miscellaneous databases

PROiPR:P29387
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027669 Expressed in 284 organ(s), highest expression level in urinary bladder
ExpressionAtlasiP29387 baseline and differential
GenevisibleiP29387 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR016346 Guanine_nucleotide-bd_bsu
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19850 PTHR19850, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 7 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGBB4_MOUSE
AccessioniPrimary (citable) accession number: P29387
Secondary accession number(s): Q3TJJ1, Q8R475, Q9JHX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 157 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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