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Protein

DNA-3-methyladenine glycosylase

Gene

MPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Activity regulationi

Binding to SSBP1 in mitochondria inhibits glycosylase activity in the context of a single-stranded DNA (ssDNA), but not a double-stranded DNA (dsDNA) substrates.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BRENDAi3.2.2.21 2681
ReactomeiR-HSA-110330 Recognition and association of DNA glycosylase with site containing an affected purine
R-HSA-110331 Cleavage of the damaged purine
R-HSA-110357 Displacement of DNA glycosylase by APEX1
SABIO-RKiP29372

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-alkyladenine DNA glycosylase
3-methyladenine DNA glycosidase
ADPG
N-methylpurine-DNA glycosylase
Gene namesi
Name:MPG
Synonyms:AAG, ANPG, MID1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103152.11
HGNCiHGNC:7211 MPG
MIMi156565 gene
neXtProtiNX_P29372

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4350
OpenTargetsiENSG00000103152
PharmGKBiPA30917

Chemistry databases

ChEMBLiCHEMBL3396943

Polymorphism and mutation databases

BioMutaiMPG

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
ChainiPRO_000010006518 – 298DNA-3-methyladenine glycosylaseAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP29372
PaxDbiP29372
PeptideAtlasiP29372
PRIDEiP29372
ProteomicsDBi54553
54554 [P29372-2]
TopDownProteomicsiP29372-1 [P29372-1]

PTM databases

iPTMnetiP29372
PhosphoSitePlusiP29372

Expressioni

Gene expression databases

BgeeiENSG00000103152 Expressed in 222 organ(s), highest expression level in tibial artery
CleanExiHS_MID1
HS_MPG
ExpressionAtlasiP29372 baseline and differential
GenevisibleiP29372 HS

Organism-specific databases

HPAiHPA006531

Interactioni

Subunit structurei

Binds MBD1. Binds SSBP1.

Binary interactionsi

Protein-protein interaction databases

BioGridi110490, 70 interactors
IntActiP29372, 15 interactors
MINTiP29372
STRINGi9606.ENSP00000219431

Chemistry databases

BindingDBiP29372

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP29372
SMRiP29372
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29372

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA glycosylase MPG family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4486 Eukaryota
COG2094 LUCA
GeneTreeiENSGT00390000009825
HOGENOMiHOG000224224
HOVERGENiHBG000019
InParanoidiP29372
KOiK03652
OMAiVEAYHHT
OrthoDBiEOG091G0NE4
PhylomeDBiP29372
TreeFamiTF331768

Family and domain databases

CDDicd00540 AAG, 1 hit
Gene3Di3.10.300.10, 1 hit
HAMAPiMF_00527 3MGH, 1 hit
InterProiView protein in InterPro
IPR011034 Formyl_transferase-like_C_sf
IPR003180 MPG
IPR036995 MPG_sf
PANTHERiPTHR10429 PTHR10429, 1 hit
PfamiView protein in Pfam
PF02245 Pur_DNA_glyco, 1 hit
SUPFAMiSSF50486 SSF50486, 1 hit
TIGRFAMsiTIGR00567 3mg, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Experimental confirmation may be lacking for some isoforms.

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P29372-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVTPALQMKK PKQFCRRMGQ KKQRPARAGQ PHSSSDAAQA PAEQPHSSSD
60 70 80 90 100
AAQAPCPRER CLGPPTTPGP YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR
110 120 130 140 150
AFLGQVLVRR LPNGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF
160 170 180 190 200
MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGL ETMRQLRSTL
210 220 230 240 250
RKGTASRVLK DRELCSGPSK LCQALAINKS FDQRDLAQDE AVWLERGPLE
260 270 280 290
PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDTQA
Length:298
Mass (Da):32,869
Last modified:September 23, 2008 - v3
Checksum:iBEA8C4CB250D572B
GO
Isoform 2 (identifier: P29372-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MVTPALQMKKPK → MPARSGA
     195-196: QL → HV

Show »
Length:293
Mass (Da):32,181
Checksum:i81BE31859298D43C
GO
Isoform 3 (identifier: P29372-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MVTPALQMKKPK → MPARSGA

Show »
Length:293
Mass (Da):32,186
Checksum:i6F710D76409B2D81
GO
Isoform 4 (identifier: P29372-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Gene prediction based on EST data.
Show »
Length:281
Mass (Da):30,824
Checksum:i6826A6C692AABA9C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2IDA3A2IDA3_HUMAN
DNA-3-methyladenine glycosylase
MPG Z69720.1-004
251Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13Q → QV in AAK61213 (PubMed:11157797).Curated1
Sequence conflicti29 – 31GQP → ARA in AAB19537 (PubMed:1874728).Curated3
Sequence conflicti44Q → R in AAB19537 (PubMed:1874728).Curated1
Sequence conflicti69 – 71GPY → SKD in AAA58369 (PubMed:1645538).Curated3
Sequence conflicti69 – 71GPY → SKD in CAA39875 (PubMed:1645538).Curated3
Sequence conflicti82H → L in AAA58369 (PubMed:1645538).Curated1
Sequence conflicti82H → L in CAA39875 (PubMed:1645538).Curated1
Sequence conflicti134A → P in AAA58627 (PubMed:1924375).Curated1
Sequence conflicti287V → E in AAA58369 (PubMed:1645538).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01913822K → Q1 PublicationCorresponds to variant dbSNP:rs3176383Ensembl.1
Natural variantiVAR_01483164P → L. Corresponds to variant dbSNP:rs2308315Ensembl.1
Natural variantiVAR_01483271Y → H1 PublicationCorresponds to variant dbSNP:rs2266607Ensembl.1
Natural variantiVAR_05009693Q → R. Corresponds to variant dbSNP:rs25671Ensembl.1
Natural variantiVAR_014833120R → C. Corresponds to variant dbSNP:rs2308313Ensembl.1
Natural variantiVAR_014834141R → Q. Corresponds to variant dbSNP:rs2308312Ensembl.1
Natural variantiVAR_014835258A → V1 PublicationCorresponds to variant dbSNP:rs769193Ensembl.1
Natural variantiVAR_014836298A → S1 PublicationCorresponds to variant dbSNP:rs2234949Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0466781 – 17Missing in isoform 4. CuratedAdd BLAST17
Alternative sequenceiVSP_0032491 – 12MVTPA…MKKPK → MPARSGA in isoform 2 and isoform 3. 3 PublicationsAdd BLAST12
Alternative sequenceiVSP_035485195 – 196QL → HV in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74905 mRNA Translation: AAA58627.1
L10752 mRNA Translation: AAF77073.1
AY258284 mRNA Translation: AAP82229.1
AY305873 mRNA Translation: AAQ95215.1
AF499437 Genomic DNA Translation: AAM14628.1
AE006462 Genomic DNA Translation: AAK61213.1
Z69720 Genomic DNA Translation: CAA93540.1
Z69720 Genomic DNA Translation: CAI95610.1
CH471112 Genomic DNA Translation: EAW85871.1
BC014991 mRNA Translation: AAH14991.1
S51033 mRNA Translation: AAB19537.1
X56528 mRNA Translation: CAA39875.1
M71215 mRNA Translation: AAA58369.1
M99626 mRNA Translation: AAB46421.1
CCDSiCCDS32345.1 [P29372-4]
CCDS32346.1 [P29372-1]
CCDS42087.1 [P29372-5]
PIRiA40798
A41230
A47471
JN0062
RefSeqiNP_001015052.1, NM_001015052.2 [P29372-4]
NP_001015054.1, NM_001015054.2 [P29372-5]
NP_002425.2, NM_002434.3 [P29372-1]
UniGeneiHs.459596

Genome annotation databases

EnsembliENST00000219431; ENSP00000219431; ENSG00000103152 [P29372-1]
ENST00000356432; ENSP00000348809; ENSG00000103152 [P29372-4]
ENST00000397817; ENSP00000380918; ENSG00000103152 [P29372-5]
GeneIDi4350
KEGGihsa:4350
UCSCiuc002cfm.4 human [P29372-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74905 mRNA Translation: AAA58627.1
L10752 mRNA Translation: AAF77073.1
AY258284 mRNA Translation: AAP82229.1
AY305873 mRNA Translation: AAQ95215.1
AF499437 Genomic DNA Translation: AAM14628.1
AE006462 Genomic DNA Translation: AAK61213.1
Z69720 Genomic DNA Translation: CAA93540.1
Z69720 Genomic DNA Translation: CAI95610.1
CH471112 Genomic DNA Translation: EAW85871.1
BC014991 mRNA Translation: AAH14991.1
S51033 mRNA Translation: AAB19537.1
X56528 mRNA Translation: CAA39875.1
M71215 mRNA Translation: AAA58369.1
M99626 mRNA Translation: AAB46421.1
CCDSiCCDS32345.1 [P29372-4]
CCDS32346.1 [P29372-1]
CCDS42087.1 [P29372-5]
PIRiA40798
A41230
A47471
JN0062
RefSeqiNP_001015052.1, NM_001015052.2 [P29372-4]
NP_001015054.1, NM_001015054.2 [P29372-5]
NP_002425.2, NM_002434.3 [P29372-1]
UniGeneiHs.459596

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BNKX-ray2.70A80-295[»]
1EWNX-ray2.10A80-298[»]
1F4RX-ray2.40A80-298[»]
1F6OX-ray2.40A80-298[»]
3QI5X-ray2.20A/B84-298[»]
3UBYX-ray2.00A/B84-298[»]
ProteinModelPortaliP29372
SMRiP29372
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110490, 70 interactors
IntActiP29372, 15 interactors
MINTiP29372
STRINGi9606.ENSP00000219431

Chemistry databases

BindingDBiP29372
ChEMBLiCHEMBL3396943

PTM databases

iPTMnetiP29372
PhosphoSitePlusiP29372

Polymorphism and mutation databases

BioMutaiMPG

Proteomic databases

EPDiP29372
PaxDbiP29372
PeptideAtlasiP29372
PRIDEiP29372
ProteomicsDBi54553
54554 [P29372-2]
TopDownProteomicsiP29372-1 [P29372-1]

Protocols and materials databases

DNASUi4350
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219431; ENSP00000219431; ENSG00000103152 [P29372-1]
ENST00000356432; ENSP00000348809; ENSG00000103152 [P29372-4]
ENST00000397817; ENSP00000380918; ENSG00000103152 [P29372-5]
GeneIDi4350
KEGGihsa:4350
UCSCiuc002cfm.4 human [P29372-1]

Organism-specific databases

CTDi4350
DisGeNETi4350
EuPathDBiHostDB:ENSG00000103152.11
GeneCardsiMPG
HGNCiHGNC:7211 MPG
HPAiHPA006531
MIMi156565 gene
neXtProtiNX_P29372
OpenTargetsiENSG00000103152
PharmGKBiPA30917
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4486 Eukaryota
COG2094 LUCA
GeneTreeiENSGT00390000009825
HOGENOMiHOG000224224
HOVERGENiHBG000019
InParanoidiP29372
KOiK03652
OMAiVEAYHHT
OrthoDBiEOG091G0NE4
PhylomeDBiP29372
TreeFamiTF331768

Enzyme and pathway databases

BRENDAi3.2.2.21 2681
ReactomeiR-HSA-110330 Recognition and association of DNA glycosylase with site containing an affected purine
R-HSA-110331 Cleavage of the damaged purine
R-HSA-110357 Displacement of DNA glycosylase by APEX1
SABIO-RKiP29372

Miscellaneous databases

ChiTaRSiMPG human
EvolutionaryTraceiP29372
GeneWikiiMPG_(gene)
GenomeRNAii4350
PROiPR:P29372
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103152 Expressed in 222 organ(s), highest expression level in tibial artery
CleanExiHS_MID1
HS_MPG
ExpressionAtlasiP29372 baseline and differential
GenevisibleiP29372 HS

Family and domain databases

CDDicd00540 AAG, 1 hit
Gene3Di3.10.300.10, 1 hit
HAMAPiMF_00527 3MGH, 1 hit
InterProiView protein in InterPro
IPR011034 Formyl_transferase-like_C_sf
IPR003180 MPG
IPR036995 MPG_sf
PANTHERiPTHR10429 PTHR10429, 1 hit
PfamiView protein in Pfam
PF02245 Pur_DNA_glyco, 1 hit
SUPFAMiSSF50486 SSF50486, 1 hit
TIGRFAMsiTIGR00567 3mg, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3MG_HUMAN
AccessioniPrimary (citable) accession number: P29372
Secondary accession number(s): G5E9E2
, Q13770, Q15275, Q15961, Q5J9I4, Q96BZ6, Q96S33, Q9NNX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 23, 2008
Last modified: October 10, 2018
This is version 200 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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