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Protein

Fructose-bisphosphate aldolase, cytoplasmic isozyme

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (SOVF_097210), Glucose-6-phosphate isomerase (GPIP)
  3. ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA)
  4. Fructose-bisphosphate aldolase, chloroplastic, Fructose-bisphosphate aldolase, cytoplasmic isozyme, Fructose-bisphosphate aldolase (SOVF_128030), Fructose-bisphosphate aldolase (SOVF_146670), Fructose-bisphosphate aldolase (SOVF_190580), Fructose-bisphosphate aldolase (SOVF_158110), Fructose-bisphosphate aldolase (SOVF_094960), Fructose-bisphosphate aldolase (SOVF_205230)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53SubstrateBy similarity1
Binding sitei142SubstrateBy similarity1
Active sitei183Proton acceptorBy similarity1
Active sitei225Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Sitei357Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: AgBase
  • protein self-association Source: AgBase

GO - Biological processi

  • fructose 1,6-bisphosphate metabolic process Source: AgBase
  • glycolytic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12898
SABIO-RKiP29356
UniPathwayi
UPA00109;UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase, cytoplasmic isozyme (EC:4.1.2.13)
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002169251 – 357Fructose-bisphosphate aldolase, cytoplasmic isozymeAdd BLAST357

Proteomic databases

PRIDEiP29356

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP29356
SMRiP29356
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequencei

Sequence statusi: Complete.

P29356-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAYRGKYAD ELIANASYIA TPGKVILAAD ESTGTIGKRF PSINVENVES
60 70 80 90 100
NRRALRELLF TTPGALPYLS GVILFEETLY QKTADGKPFV DAMKDGGVLP
110 120 130 140 150
GIKVDKGTVE LAGTNGETTT QGLDGLAQRC AQYYTAGARF AKWRAVLKIG
160 170 180 190 200
PTEPSPLAIL ENANGLARYG IICQENGLVP IVEPEILVDG THDIDRCAEV
210 220 230 240 250
SERVLAACYK ALNDHHVLLE GTSLKPNIVT PGSESKKVTP EVIAEYTVRT
260 270 280 290 300
LQRTVPQAVP GVMFLSGGQS EEEATLNLNA MNKLETKKPW TLSFSYGRAL
310 320 330 340 350
QQSTLKAWQG KEENVAKAQE VFLARAKGNS EATLGKYQGG AGGADASESL

HVKDYKY
Length:357
Mass (Da):38,471
Last modified:December 1, 1992 - v1
Checksum:i33150E3D80B1C6DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65742 mRNA Translation: CAA46649.1
PIRiS31091 ADSPAC

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65742 mRNA Translation: CAA46649.1
PIRiS31091 ADSPAC

3D structure databases

ProteinModelPortaliP29356
SMRiP29356
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP29356

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00183

BioCyciMetaCyc:MONOMER-12898
SABIO-RKiP29356

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALF_SPIOL
AccessioniPrimary (citable) accession number: P29356
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 25, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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