UniProtKB - P29321 (EPHA8_RAT)
Ephrin type-A receptor 8
Epha8
Functioni
Catalytic activityi
- EC:2.7.10.1PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 34 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 127 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 8 – 16 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- GPI-linked ephrin receptor activity Source: UniProtKB
- transmembrane-ephrin receptor activity Source: GO_Central
- transmembrane receptor protein tyrosine kinase activity Source: GO_Central
GO - Biological processi
- axon guidance Source: UniProtKB
- cell adhesion Source: UniProtKB-KW
- cellular response to follicle-stimulating hormone stimulus Source: RGD
- ephrin receptor signaling pathway Source: UniProtKB
- multicellular organism development Source: GO_Central
- neuron projection development Source: UniProtKB
- neuron remodeling Source: UniProtKB
- positive regulation of kinase activity Source: GO_Central
- positive regulation of MAPK cascade Source: UniProtKB
- positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- regulation of cell adhesion Source: UniProtKB
- regulation of cell adhesion mediated by integrin Source: UniProtKB
- substrate-dependent cell migration Source: UniProtKB
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase |
Biological process | Cell adhesion, Neurogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Ephrin type-A receptor 8 (EC:2.7.10.1)Alternative name(s): EPH- and ELK-related kinase Tyrosine-protein kinase receptor EEK |
Gene namesi | Name:Epha8 Synonyms:Eek |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708543, Epha8 |
Subcellular locationi
Endosome
- Early endosome membrane By similarity
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Other locations
- Cell projection By similarity
Note: Undergoes clathrin-mediated endocytosis upon EFNA5-binding and is targeted to early endosomes.By similarity
Endosome
- early endosome membrane Source: UniProtKB-SubCell
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Other locations
- neuron projection Source: UniProtKB
- receptor complex Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cell projection, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160273 | ‹1 – 372 | Ephrin type-A receptor 8Add BLAST | ›372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 206 | Phosphotyrosine; by autocatalysisBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P29321 |
PRIDEi | P29321 |
PTM databases
PhosphoSitePlusi | P29321 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. May also form heterodimers with other ephrin receptors.
Interacts with FYN; possible downstream effector of EPHA8 in regulation of cell adhesion.
Interacts with PIK3CG; regulates integrin-mediated cell adhesion to substrate.
Interacts with TIAM1; regulates clathrin-mediated endocytosis of EPHA8.
Interacts with ANKS1A and ANKS1B; EPHA8 kinase activity-independent but stimulated by EPHA8 ubiquitination (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017559 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 263 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 262 | |
Domaini | 297 – 372 | SAMPROSITE-ProRule annotationAdd BLAST | 76 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 370 – 372 | PDZ-bindingSequence analysis | 3 |
Phylogenomic databases
eggNOGi | KOG0196, Eukaryota |
InParanoidi | P29321 |
OMAi | PMGCPTA |
PhylomeDBi | P29321 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR020691, EphrinA_rcpt8 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
PANTHERi | PTHR24416:SF339, PTHR24416:SF339, 1 hit |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50105, SAM_DOMAIN, 1 hit |
(1+)i Sequence
Sequence statusi: Fragment.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
RIHIEKIIGS GESGEVCYGR LQVPGQRDVP VAIKALKAGY TERQRQDFLR
60 70 80 90 100
EAAIMGQFDH PNIIRLEGVV TRGRLAMIVT EYMENGSLDA FLRTHDGQFT
110 120 130 140 150
ILQLVGMLKG VGAGMRYLSD LGYIHRDLAA RNILVDGRLV CKVSDFGLSR
160 170 180 190 200
ALEDDPEAAY TTAGGKIPIR WTAPEAIAFR TFSSASDVWS FGVVMWEVLA
210 220 230 240 250
YGERPYWNMT NQDVISSVEE GYRLPAPMGC PRALHQLMLD CWHKDRAQRP
260 270 280 290 300
RFSHVVSVLE ALVHSPESLR ATATVSRCPA PAFARSCFDL RAGGNGNGDL
310 320 330 340 350
TVGDWLDSIR MGRYRDHFAA GGYSSLGMVL HMNAQDVRAL GITLMGHQKK
360 370
ILGSIQTMRS QLSCTQGPRR HL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1LMX3 | F1LMX3_RAT | Receptor protein-tyrosine kinase | Epha8 | 978 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59290 mRNA Translation: CAA41979.1 |
PIRi | S23363 |
Genome annotation databases
UCSCi | RGD:708543, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59290 mRNA Translation: CAA41979.1 |
PIRi | S23363 |
3D structure databases
SMRi | P29321 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017559 |
PTM databases
PhosphoSitePlusi | P29321 |
Proteomic databases
PaxDbi | P29321 |
PRIDEi | P29321 |
Genome annotation databases
UCSCi | RGD:708543, rat |
Organism-specific databases
RGDi | 708543, Epha8 |
Phylogenomic databases
eggNOGi | KOG0196, Eukaryota |
InParanoidi | P29321 |
OMAi | PMGCPTA |
PhylomeDBi | P29321 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR020691, EphrinA_rcpt8 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
PANTHERi | PTHR24416:SF339, PTHR24416:SF339, 1 hit |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50105, SAM_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EPHA8_RAT | |
Accessioni | P29321Primary (citable) accession number: P29321 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |