UniProtKB - P29319 (EPHA3_MOUSE)
Ephrin type-A receptor 3
Epha3
Functioni
Catalytic activityi
- EC:2.7.10.1PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 653 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 746 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 628 – 633 | ATPPROSITE-ProRule annotation | 6 | |
Nucleotide bindingi | 700 – 706 | ATPPROSITE-ProRule annotation | 7 | |
Nucleotide bindingi | 750 – 751 | ATPPROSITE-ProRule annotation | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ephrin receptor activity Source: MGI
- GPI-linked ephrin receptor activity Source: UniProtKB
- transmembrane-ephrin receptor activity Source: GO_Central
- transmembrane receptor protein tyrosine kinase activity Source: GO_Central
GO - Biological processi
- axon guidance Source: GO_Central
- cell migration Source: UniProtKB
- cellular response to follicle-stimulating hormone stimulus Source: MGI
- cellular response to retinoic acid Source: MGI
- endocardial cushion development Source: UniProtKB
- ephrin receptor signaling pathway Source: UniProtKB
- fasciculation of motor neuron axon Source: UniProtKB
- fasciculation of sensory neuron axon Source: UniProtKB
- multicellular organism development Source: GO_Central
- negative regulation of endocytosis Source: MGI
- negative regulation of neuron projection development Source: MGI
- peptidyl-tyrosine phosphorylation Source: MGI
- positive regulation of kinase activity Source: GO_Central
- positive regulation of neuron projection development Source: MGI
- positive regulation of protein localization to plasma membrane Source: MGI
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of cell migration Source: MGI
- regulation of epithelial to mesenchymal transition Source: UniProtKB
- regulation of focal adhesion assembly Source: UniProtKB
- regulation of GTPase activity Source: UniProtKB
- regulation of microtubule cytoskeleton organization Source: UniProtKB
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Kinase, Receptor, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 3474 |
Reactomei | R-MMU-2682334, EPH-Ephrin signaling R-MMU-3928663, EPHA-mediated growth cone collapse R-MMU-3928665, EPH-ephrin mediated repulsion of cells |
Names & Taxonomyi
Protein namesi | Recommended name: Ephrin type-A receptor 3 (EC:2.7.10.1)Alternative name(s): EPH-like kinase 4 Short name: EK4 Short name: mEK4 Tyrosine-protein kinase TYRO4 Tyrosine-protein kinase receptor ETK1 |
Gene namesi | Name:Epha3 Synonyms:Etk1, Mek4, Tyro4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99612, Epha3 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Extracellular region or secreted
- Secreted By similarity
Cytoskeleton
- actin cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Endosome
- early endosome Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Nucleus
- nuclear membrane Source: MGI
- nucleoplasm Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- neuron projection Source: GO_Central
- receptor complex Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 540 | ExtracellularSequence analysisAdd BLAST | 520 | |
Transmembranei | 541 – 564 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 565 – 983 | CytoplasmicSequence analysisAdd BLAST | 419 |
Keywords - Cellular componenti
Cell membrane, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL2034794 |
GuidetoPHARMACOLOGYi | 1823 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | By similarityAdd BLAST | 20 | |
ChainiPRO_0000016803 | 21 – 983 | Ephrin type-A receptor 3Add BLAST | 963 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 231 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 336 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 390 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 403 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 492 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 596 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 602 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 701 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 779 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 937 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P29319 |
PaxDbi | P29319 |
PRIDEi | P29319 |
PTM databases
GlyGeni | P29319, 5 sites |
iPTMneti | P29319 |
PhosphoSitePlusi | P29319 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.
Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function.
Interacts (phosphorylated) with PTPN1; dephosphorylates EPHA3 and may regulate its trafficking and function (By similarity).
Interacts (phosphorylated) with CRK; mediates EFNA5-EPHA3 signaling through RHOA GTPase activation.
Interacts with NCK1 (via SH2 domain); mediates EFNA5-EPHA3 signaling.
By similarity2 PublicationsProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000066554 |
Chemistry databases
BindingDBi | P29319 |
Miscellaneous databases
RNActi | P29319, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 206 | Eph LBDPROSITE-ProRule annotationAdd BLAST | 178 | |
Domaini | 324 – 434 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 111 | |
Domaini | 435 – 530 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 621 – 882 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 262 | |
Domaini | 911 – 975 | SAMPROSITE-ProRule annotationAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 981 – 983 | PDZ-bindingSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 188 – 321 | Cys-richAdd BLAST | 134 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0196, Eukaryota |
InParanoidi | P29319 |
PhylomeDBi | P29319 |
Family and domain databases
CDDi | cd10481, EphR_LBD_A3, 1 hit cd00063, FN3, 2 hits |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR034266, EphA3_rcpt_lig-bd IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR011641, Tyr-kin_ephrin_A/B_rcpt-like IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF07699, Ephrin_rec_like, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF07647, SAM_2, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM01411, Ephrin_rec_like, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00790, RECEPTOR_TYR_KIN_V_1, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDCHLSILVL LGCCVLSCSG ELSPQPSNEV NLLDSKTIQG ELGWISYPSH
60 70 80 90 100
GWEEISGVDE HYTPIRTYQV CNVMDHSQNN WLRTNWVPRN SAQKIYVELK
110 120 130 140 150
FTLRDCNSIP LVLGTCKETF NLYYMESDDH GVKFREHQFT KIDTIAADES
160 170 180 190 200
FTQMDLGDRI LKLNTEIREV GPVNKKGFYL AFQDVGACVA LVSVRVYFKK
210 220 230 240 250
CPFTVKNLAM FPDTVPMDSQ SLVEVRGSCV NNSKEEDPPR MYCSTEGEWL
260 270 280 290 300
VPIGKCTCNA GYEERGFICQ ACRPGFYKAS DGAAKCAKCP PHSSTQEDGS
310 320 330 340 350
MNCRCENNYF RAEKDPPSMA CARPPSAPRN VISNINETSV ILDWSWPLDT
360 370 380 390 400
GGRKDITFNI ICKKCGWNVR QCEPCSPNVR FLPRQLGLTN TTVTVTDLLA
410 420 430 440 450
HTNYTFEIDA VNGVSELSSP PRQYAAVSIT TNQAAPSPVM TIKKDRTSRN
460 470 480 490 500
SISLSWQEPE HPNGIILDYE VKYYQKQEQE TSYTILRARG TNVTISSLKP
510 520 530 540 550
DTTYVFQIRA RTAAGYGTNS RKFEFETSPD SFSISGENSH VVMIAISAAV
560 570 580 590 600
AIIVLTVVTY VLVGRFCGYH KSKHSAEEKR LHFGNGHLKL PGLRTYVDPH
610 620 630 640 650
TYEDPTQAVH EFAKELDATN ISIDKVVGAG EFGEVCSGRL KLPSKKEISV
660 670 680 690 700
AIKTLKVGYT EKQRRDFLGE ASIMGQFDHP NIIRLEGVVT KSKPEMIVTE
710 720 730 740 750
YMENGSLDSF LRKHDAQFTV IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR
760 770 780 790 800
NILINSNLVC KVSDFGLSRV LEDDPEAAYT TRGGKIPIRW TSPEAMSYRK
810 820 830 840 850
FTSASDVWSY GIVLWEVMSY GERPYSQMSN QDVIKAVDER YRLPPPMDCP
860 870 880 890 900
AALYQLMLDC WQKDRNNRPK FEQIVSILDK LIRNPGSLKI ITSAAARPSN
910 920 930 940 950
LLLDQSNVDI ATFHTTGDWL NGMRTAHCKE IFTGVEYSSC DTIAKISTDD
960 970 980
MKKVGVTVVG PQKKIISTIK ALETQSKNGP VPV
The sequence of this isoform differs from the canonical sequence as follows:
531-537: SFSISGE → CMYYFSF
538-983: Missing.
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8BRB1 | Q8BRB1_MOUSE | Receptor protein-tyrosine kinase | Epha3 | 984 | Annotation score: | ||
B7ZNN5 | B7ZNN5_MOUSE | Receptor protein-tyrosine kinase | Epha3 | 983 | Annotation score: | ||
Q8C9K6 | Q8C9K6_MOUSE | Ephrin type-A receptor 3 | Epha3 | 538 | Annotation score: | ||
A0A338P7J9 | A0A338P7J9_MOUSE | Ephrin type-A receptor 3 | Epha3 | 83 | Annotation score: | ||
A0A338P6A7 | A0A338P6A7_MOUSE | Ephrin type-A receptor 3 | Epha3 | 204 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041882 | 531 – 537 | SFSISGE → CMYYFSF in isoform Short. 1 Publication | 7 | |
Alternative sequenceiVSP_041883 | 538 – 983 | Missing in isoform Short. 1 PublicationAdd BLAST | 446 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68513 mRNA Translation: AAA39521.1 M68515 mRNA Translation: AAA39522.1 |
PIRi | A45583 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68513 mRNA Translation: AAA39521.1 M68515 mRNA Translation: AAA39522.1 |
PIRi | A45583 |
3D structure databases
SMRi | P29319 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000066554 |
Chemistry databases
BindingDBi | P29319 |
ChEMBLi | CHEMBL2034794 |
GuidetoPHARMACOLOGYi | 1823 |
PTM databases
GlyGeni | P29319, 5 sites |
iPTMneti | P29319 |
PhosphoSitePlusi | P29319 |
Proteomic databases
MaxQBi | P29319 |
PaxDbi | P29319 |
PRIDEi | P29319 |
Organism-specific databases
MGIi | MGI:99612, Epha3 |
Phylogenomic databases
eggNOGi | KOG0196, Eukaryota |
InParanoidi | P29319 |
PhylomeDBi | P29319 |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 3474 |
Reactomei | R-MMU-2682334, EPH-Ephrin signaling R-MMU-3928663, EPHA-mediated growth cone collapse R-MMU-3928665, EPH-ephrin mediated repulsion of cells |
Miscellaneous databases
ChiTaRSi | Epha3, mouse |
PROi | PR:P29319 |
RNActi | P29319, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd10481, EphR_LBD_A3, 1 hit cd00063, FN3, 2 hits |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR034266, EphA3_rcpt_lig-bd IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR011641, Tyr-kin_ephrin_A/B_rcpt-like IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF07699, Ephrin_rec_like, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF07647, SAM_2, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM01411, Ephrin_rec_like, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00790, RECEPTOR_TYR_KIN_V_1, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EPHA3_MOUSE | |
Accessioni | P29319Primary (citable) accession number: P29319 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | October 7, 2020 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index