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UniProtKB - P29295 (HRR25_YEAST)
Protein
Casein kinase I homolog HRR25
Gene
HRR25
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Protein kinase which phosphorylates serine and threonine residues (PubMed:1495994).
Can use casein as a substrate (PubMed:1495994).
Phosphorylates elongator complex member ELP1/IKI3 on 'Ser-1198' and 'Ser-1202' which promotes the tRNA modification function of the complex (PubMed:25569479).
Associated with repair of damaged DNA and meiosis (PubMed:1887218).
3 PublicationsMiscellaneous
Present with 10300 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 38 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 128 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 23 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: SGD
- protein serine kinase activity Source: RHEA
- protein tyrosine kinase activity Source: SGD
GO - Biological processi
- autophagy of peroxisome Source: SGD
- DNA repair Source: SGD
- endocytosis Source: GO_Central
- monopolar spindle attachment to meiosis I kinetochore Source: SGD
- peptidyl-serine phosphorylation Source: GO_Central
- peptidyl-tyrosine autophosphorylation Source: SGD
- peptidyl-tyrosine phosphorylation Source: SGD
- positive regulation of clathrin-dependent endocytosis Source: SGD
- protein phosphorylation Source: SGD
- regulation of autophagosome assembly Source: SGD
- regulation of ER to Golgi vesicle-mediated transport Source: SGD
- regulation of protein localization Source: SGD
- regulation of protein localization by the Cvt pathway Source: SGD
- regulation of vesicle fusion with Golgi apparatus Source: SGD
- ribosomal large subunit biogenesis Source: SGD
- ribosomal small subunit biogenesis Source: SGD
- tRNA wobble uridine modification Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Reactomei | R-SCE-204005, COPII-mediated vesicle transport R-SCE-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:HRR25 Ordered Locus Names:YPL204W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006125, HRR25 |
VEuPathDBi | FungiDB:YPL204W |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
- nucleoplasm 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- spindle pole body Source: SGD
Cytosol
- cytosol Source: SGD
Golgi apparatus
- Golgi apparatus Source: SGD
Nucleus
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cellular bud neck Source: SGD
- cellular bud tip Source: SGD
- chromosome, centromeric region Source: SGD
- cytoplasm Source: SGD
- monopolin complex Source: SGD
- phagophore assembly site Source: SGD
- preribosome, small subunit precursor Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192859 | 1 – 494 | Casein kinase I homolog HRR25Add BLAST | 494 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 143 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P29295 |
PaxDbi | P29295 |
PRIDEi | P29295 |
PTM databases
iPTMneti | P29295 |
Interactioni
Subunit structurei
Binary interactionsi
P29295
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 35980, 621 interactors |
ComplexPortali | CPX-1681, Monopolin complex |
DIPi | DIP-157N |
IntActi | P29295, 151 interactors |
MINTi | P29295 |
STRINGi | 4932.YPL204W |
Miscellaneous databases
RNActi | P29295, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P29295 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 278 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 270 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 394 – 494 | DisorderedSequence analysisAdd BLAST | 101 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 394 – 487 | Polar residuesSequence analysisAdd BLAST | 94 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000176141 |
HOGENOMi | CLU_019279_2_7_1 |
InParanoidi | P29295 |
OMAi | ESRVYKY |
Family and domain databases
InterProi | View protein in InterPro IPR030509, CK1 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR11909:SF409, PTHR11909:SF409, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P29295-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLRVGRKFR IGRKIGSGSF GDIYHGTNLI SGEEVAIKLE SIRSRHPQLD
60 70 80 90 100
YESRVYRYLS GGVGIPFIRW FGREGEYNAM VIDLLGPSLE DLFNYCHRRF
110 120 130 140 150
SFKTVIMLAL QMFCRIQYIH GRSFIHRDIK PDNFLMGVGR RGSTVHVIDF
160 170 180 190 200
GLSKKYRDFN THRHIPYREN KSLTGTARYA SVNTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLIYFCKGS LPWQGLKATT KKQKYDRIME KKLNVSVETL CSGLPLEFQE
260 270 280 290 300
YMAYCKNLKF DEKPDYLFLA RLFKDLSIKL EYHNDHLFDW TMLRYTKAMV
310 320 330 340 350
EKQRDLLIEK GDLNANSNAA SASNSTDNKS ETFNKIKLLA MKKFPTHFHY
360 370 380 390 400
YKNEDKHNPS PEEIKQQTIL NNNAASSLPE ELLNALDKGM ENLRQQQPQQ
410 420 430 440 450
QVQSSQPQPQ PQQLQQQPNG QRPNYYPEPL LQQQQRDSQE QQQQVPMATT
460 470 480 490
RATQYPPQIN SNNFNTNQAS VPPQMRSNPQ QPPQDKPAGQ SIWL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68605 Genomic DNA Translation: AAA34687.1 Z73560 Genomic DNA Translation: CAA97918.1 BK006949 Genomic DNA Translation: DAA11231.1 |
PIRi | A40860 |
RefSeqi | NP_015120.1, NM_001184018.1 |
Genome annotation databases
EnsemblFungii | YPL204W_mRNA; YPL204W; YPL204W |
GeneIDi | 855897 |
KEGGi | sce:YPL204W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68605 Genomic DNA Translation: AAA34687.1 Z73560 Genomic DNA Translation: CAA97918.1 BK006949 Genomic DNA Translation: DAA11231.1 |
PIRi | A40860 |
RefSeqi | NP_015120.1, NM_001184018.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4XHL | X-ray | 3.01 | A | 1-394 | [»] | |
5CYZ | X-ray | 1.84 | A | 1-394 | [»] | |
5CZO | X-ray | 2.89 | A/B | 1-394 | [»] | |
SMRi | P29295 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35980, 621 interactors |
ComplexPortali | CPX-1681, Monopolin complex |
DIPi | DIP-157N |
IntActi | P29295, 151 interactors |
MINTi | P29295 |
STRINGi | 4932.YPL204W |
PTM databases
iPTMneti | P29295 |
Proteomic databases
MaxQBi | P29295 |
PaxDbi | P29295 |
PRIDEi | P29295 |
Genome annotation databases
EnsemblFungii | YPL204W_mRNA; YPL204W; YPL204W |
GeneIDi | 855897 |
KEGGi | sce:YPL204W |
Organism-specific databases
SGDi | S000006125, HRR25 |
VEuPathDBi | FungiDB:YPL204W |
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000176141 |
HOGENOMi | CLU_019279_2_7_1 |
InParanoidi | P29295 |
OMAi | ESRVYKY |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Reactomei | R-SCE-204005, COPII-mediated vesicle transport R-SCE-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Miscellaneous databases
PROi | PR:P29295 |
RNActi | P29295, protein |
Family and domain databases
InterProi | View protein in InterPro IPR030509, CK1 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR11909:SF409, PTHR11909:SF409, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HRR25_YEAST | |
Accessioni | P29295Primary (citable) accession number: P29295 Secondary accession number(s): D6W3G5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 200 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families