UniProtKB - P29293 (ACRO_RAT)
Protein
Acrosin
Gene
Acr
Organism
Rattus norvegicus (Rat)
Status
Functioni
Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. EC:3.4.21.10
Activity regulationi
Inhibited by SERPINA5.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 89 | Charge relay systemBy similarity | 1 | |
Active sitei | 143 | Charge relay systemBy similarity | 1 | |
Active sitei | 241 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- amidase activity Source: UniProtKB
- fucose binding Source: UniProtKB
- mannose binding Source: UniProtKB
- peptidase activity Source: RGD
- serine-type endopeptidase activity Source: UniProtKB
- serine-type peptidase activity Source: UniProtKB
GO - Biological processi
- acrosome matrix dispersal Source: RGD
- acrosome reaction Source: UniProtKB
- activation of adenylate cyclase activity Source: UniProtKB
- binding of sperm to zona pellucida Source: RGD
- penetration of zona pellucida Source: RGD
- protein catabolic process Source: RGD
- response to steroid hormone Source: RGD
- single fertilization Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BRENDAi | 3.4.21.10, 5301 |
Reactomei | R-RNO-1300645, Acrosome Reaction and Sperm:Oocyte Membrane Binding |
Protein family/group databases
MEROPSi | S01.223 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Acr |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2024, Acr |
Subcellular locationi
Other locations
- acrosomal matrix Source: HGNC-UCL
- acrosomal vesicle Source: RGD
- Golgi-associated vesicle Source: RGD
- protein-containing complex Source: RGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | By similarityAdd BLAST | 19 | |
ChainiPRO_0000027534 | 20 – 437 | AcrosinAdd BLAST | 418 | |
ChainiPRO_0000027535 | 20 – 42 | Acrosin light chainAdd BLAST | 23 | |
ChainiPRO_0000027536 | 43 – 345 | Acrosin heavy chainAdd BLAST | 303 | |
PropeptideiPRO_0000027537 | 346 – 437 | Pro-richBy similarityAdd BLAST | 92 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 22 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 25 ↔ 155 | Interchain (between light and heavy chains)PROSITE-ProRule annotation | ||
Disulfide bondi | 29 ↔ 163 | Interchain (between light and heavy chains)PROSITE-ProRule annotation | ||
Disulfide bondi | 74 ↔ 90 | PROSITE-ProRule annotation | ||
Disulfide bondi | 178 ↔ 247 | PROSITE-ProRule annotation | ||
Disulfide bondi | 210 ↔ 226 | PROSITE-ProRule annotation | ||
Glycosylationi | 211 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 237 ↔ 267 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P29293 |
PRIDEi | P29293 |
PTM databases
GlyGeni | P29293, 2 sites |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000029762, Expressed in testis and 17 other tissues |
ExpressionAtlasi | P29293, baseline and differential |
Genevisiblei | P29293, RN |
Interactioni
Subunit structurei
Heavy chain (catalytic) and a light chain linked by two disulfide bonds.
Forms a heterodimer with SERPINA5 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000046900 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 43 – 291 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 249 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162430 |
HOGENOMi | CLU_006842_0_4_1 |
InParanoidi | P29293 |
OMAi | SRRYHAC |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P29293 |
TreeFami | TF335943 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR012267, Pept_S1A_acrosin IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001141, Acrosin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P29293-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVEMLPTVVA LVLAVSVVAK DNTTCDGPCG LRFRQNPQAG IRIVGGQTSS
60 70 80 90 100
PGAWPWMVSL QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDW
110 120 130 140 150
RLVFGAHEIE YGRNKPVKEP QQERYVQKIV IHEKYNAVTE GNDIALLKVT
160 170 180 190 200
PPVTCGDFVG PGCLPHFKSG PPRIPHTCYV TGWGYIKDNA PRPSPVLMEA
210 220 230 240 250
RVDLIDLDLC NSTQWYNGRV TSTNVCAGYP EGKIDTCQGD SGGPLMCRDS
260 270 280 290 300
VDSPFVIVGI TSWGVGCARA KRPGVYTATW DYLDWIASKI GPTALHLIQP
310 320 330 340 350
ATPHPPTTQQ PVISFHPPSI HPPWYFQHLS PRPPHLRPPR PLLHQPSSVH
360 370 380 390 400
TSSAPVIPLL SLLTPVQPVS FTLAAYHTRH HTTLSFARRL QHLIEALKMR
410 420 430
TYPIKYPSRY SGPVNYQHRF STFEPLSNKP SEPLLHS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K321 | A0A0G2K321_RAT | Acrosin | Acr | 435 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 – 26 | Missing in CAA41441 (PubMed:1802037).Curated | 5 | |
Sequence conflicti | 51 – 52 | PG → RW (PubMed:1932123).Curated | 2 | |
Sequence conflicti | 51 – 52 | PG → RW (PubMed:1802037).Curated | 2 | |
Sequence conflicti | 95 | K → KR in CAA41441 (PubMed:1802037).Curated | 1 | |
Sequence conflicti | 250 – 253 | SVDS → TRRQ (PubMed:1932123).Curated | 4 | |
Sequence conflicti | 250 – 253 | SVDS → TRRQ (PubMed:1802037).Curated | 4 | |
Sequence conflicti | 255 – 268 | FVIVG…GVGCA → LCDRGDHELGGRLC (PubMed:1932123).CuratedAdd BLAST | 14 | |
Sequence conflicti | 255 – 268 | FVIVG…GVGCA → LCDRGDHELGGRLC (PubMed:1802037).CuratedAdd BLAST | 14 | |
Sequence conflicti | 320 – 337 | IHPPW…PPHLR → TPPSLVLPTPVSSAALPT (PubMed:1932123).CuratedAdd BLAST | 18 | |
Sequence conflicti | 320 – 337 | IHPPW…PPHLR → TPPSLVLPTPVSSAALPT (PubMed:1802037).CuratedAdd BLAST | 18 | |
Sequence conflicti | 388 – 389 | RR → SA (PubMed:1932123).Curated | 2 | |
Sequence conflicti | 388 – 389 | RR → SA (PubMed:1802037).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59254 mRNA Translation: CAA41947.1 X58550 Genomic DNA Translation: CAA41441.2 BC097345 mRNA Translation: AAH97345.1 |
PIRi | S18407 |
RefSeqi | NP_036622.2, NM_012490.2 |
Genome annotation databases
Ensembli | ENSRNOT00000045647; ENSRNOP00000046900; ENSRNOG00000029762 |
GeneIDi | 24163 |
KEGGi | rno:24163 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59254 mRNA Translation: CAA41947.1 X58550 Genomic DNA Translation: CAA41441.2 BC097345 mRNA Translation: AAH97345.1 |
PIRi | S18407 |
RefSeqi | NP_036622.2, NM_012490.2 |
3D structure databases
SMRi | P29293 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000046900 |
Protein family/group databases
MEROPSi | S01.223 |
PTM databases
GlyGeni | P29293, 2 sites |
Proteomic databases
PaxDbi | P29293 |
PRIDEi | P29293 |
Genome annotation databases
Ensembli | ENSRNOT00000045647; ENSRNOP00000046900; ENSRNOG00000029762 |
GeneIDi | 24163 |
KEGGi | rno:24163 |
Organism-specific databases
CTDi | 49 |
RGDi | 2024, Acr |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162430 |
HOGENOMi | CLU_006842_0_4_1 |
InParanoidi | P29293 |
OMAi | SRRYHAC |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P29293 |
TreeFami | TF335943 |
Enzyme and pathway databases
BRENDAi | 3.4.21.10, 5301 |
Reactomei | R-RNO-1300645, Acrosome Reaction and Sperm:Oocyte Membrane Binding |
Miscellaneous databases
PROi | PR:P29293 |
Gene expression databases
Bgeei | ENSRNOG00000029762, Expressed in testis and 17 other tissues |
ExpressionAtlasi | P29293, baseline and differential |
Genevisiblei | P29293, RN |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR012267, Pept_S1A_acrosin IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001141, Acrosin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACRO_RAT | |
Accessioni | P29293Primary (citable) accession number: P29293 Secondary accession number(s): Q4QR89, Q9QWF2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | May 16, 2006 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries