UniProtKB - P29268 (CCN2_MOUSE)
Protein
CCN family member 2
Gene
Ccn2
Organism
Mus musculus (Mouse)
Status
Functioni
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).By similarity2 Publications
GO - Molecular functioni
- fibronectin binding Source: MGI
- growth factor activity Source: MGI
- heparin binding Source: MGI
- insulin-like growth factor binding Source: InterPro
- integrin binding Source: MGI
- protein C-terminus binding Source: MGI
GO - Biological processi
- aging Source: Ensembl
- angiogenesis Source: MGI
- cartilage condensation Source: MGI
- cell adhesion Source: GO_Central
- cell differentiation Source: MGI
- cell-matrix adhesion Source: MGI
- cell migration Source: MGI
- chondrocyte proliferation Source: MGI
- connective tissue development Source: MGI
- cytosolic calcium ion transport Source: MGI
- DNA biosynthetic process Source: UniProtKB-KW
- extracellular matrix constituent secretion Source: MGI
- fibroblast growth factor receptor signaling pathway Source: MGI
- integrin-mediated signaling pathway Source: MGI
- intracellular signal transduction Source: MGI
- lung development Source: MGI
- negative regulation of cell death Source: MGI
- negative regulation of gene expression Source: MGI
- ossification Source: MGI
- positive regulation of cardiac muscle contraction Source: MGI
- positive regulation of cell activation Source: MGI
- positive regulation of cell death Source: MGI
- positive regulation of cell differentiation Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of collagen biosynthetic process Source: MGI
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of G0 to G1 transition Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of JNK cascade Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of stress fiber assembly Source: MGI
- reactive oxygen species metabolic process Source: MGI
- regulation of chondrocyte differentiation Source: MGI
- response to amino acid Source: Ensembl
- response to anoxia Source: Ensembl
- response to estradiol Source: Ensembl
- response to fatty acid Source: Ensembl
- response to glucose Source: Ensembl
- response to mineralocorticoid Source: Ensembl
- response to peptide hormone Source: Ensembl
- signal transduction Source: GO_Central
- tissue homeostasis Source: MGI
Keywordsi
Molecular function | Heparin-binding |
Biological process | Cell adhesion, DNA synthesis |
Names & Taxonomyi
Protein namesi | Recommended name: CCN family member 2Alternative name(s): Cellular communication network factor 2 Connective tissue growth factor Hypertrophic chondrocyte-specific protein 24 Protein FISP-12 |
Gene namesi | Name:Ccn2Imported Synonyms:betaIG-M21 Publication, Ctgf, Fisp-121 Publication, Fisp121 Publication, Hcs24 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95537, Ccn2 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix 1 Publication
- Secreted 1 Publication
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: BHF-UCL
Golgi apparatus
- cis-Golgi network Source: MGI
Other locations
- cell cortex Source: MGI
- extracellular matrix Source: MGI
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000014403 | 26 – 348 | CCN family member 2Add BLAST | 323 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 255 ↔ 292 | By similarity | ||
Disulfide bondi | 272 ↔ 306 | By similarity | ||
Disulfide bondi | 283 ↔ 322 | By similarity | ||
Disulfide bondi | 286 ↔ 324 | By similarity | ||
Disulfide bondi | 291 ↔ 328 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
CPTACi | non-CPTAC-3782 |
MaxQBi | P29268 |
PaxDbi | P29268 |
PeptideAtlasi | P29268 |
PRIDEi | P29268 |
ProteomicsDBi | 284143 |
PTM databases
PhosphoSitePlusi | P29268 |
Expressioni
Tissue specificityi
Testis, spleen, kidney, lung, heart, and brain (lowest level in testis and highest in lung).
Inductioni
By growth factors.
Gene expression databases
Bgeei | ENSMUSG00000019997, Expressed in decidua and 363 other tissues |
Genevisiblei | P29268, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- fibronectin binding Source: MGI
- growth factor activity Source: MGI
- insulin-like growth factor binding Source: InterPro
- integrin binding Source: MGI
- protein C-terminus binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199679, 3 interactors |
IntActi | P29268, 1 interactor |
STRINGi | 10090.ENSMUSP00000020171 |
Miscellaneous databases
RNActi | P29268, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 97 | IGFBP N-terminalPROSITE-ProRule annotationAdd BLAST | 72 | |
Domaini | 100 – 166 | VWFCPROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 197 – 242 | TSP type-1PROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 255 – 329 | CTCKPROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 246 – 348 | Heparin-bindingBy similarityAdd BLAST | 103 |
Sequence similaritiesi
Belongs to the CCN family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QQDX, Eukaryota |
GeneTreei | ENSGT00940000155019 |
HOGENOMi | CLU_063247_1_0_1 |
InParanoidi | P29268 |
OMAi | QDCGGQC |
OrthoDBi | 999958at2759 |
TreeFami | TF326070 |
Family and domain databases
Gene3Di | 2.20.100.10, 1 hit |
InterProi | View protein in InterPro IPR006207, Cys_knot_C IPR006208, Glyco_hormone_CN IPR009030, Growth_fac_rcpt_cys_sf IPR000867, IGFBP-like IPR012395, IGFBP_CNN IPR017891, Insulin_GF-bd_Cys-rich_CS IPR043973, TSP1_CCN IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf IPR001007, VWF_dom |
Pfami | View protein in Pfam PF00007, Cys_knot, 1 hit PF00219, IGFBP, 1 hit PF19035, TSP1_CCN, 1 hit PF00093, VWC, 1 hit |
PIRSFi | PIRSF036495, IGFBP_rP_CNN, 1 hit |
SMARTi | View protein in SMART SM00041, CT, 1 hit SM00121, IB, 1 hit SM00209, TSP1, 1 hit SM00214, VWC, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit SSF82895, SSF82895, 1 hit |
PROSITEi | View protein in PROSITE PS01185, CTCK_1, 1 hit PS01225, CTCK_2, 1 hit PS00222, IGFBP_N_1, 1 hit PS51323, IGFBP_N_2, 1 hit PS50092, TSP1, 1 hit PS01208, VWFC_1, 1 hit PS50184, VWFC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P29268-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLASVAGPIS LALVLLALCT RPAMGQDCSA QCQCAAEAAP HCPAGVSLVL
60 70 80 90 100
DGCGCCRVCA KQLGELCTER DPCDPHKGLF CDFGSPANRK IGVCTAKDGA
110 120 130 140 150
PCVFGGSVYR SGESFQSSCK YQCTCLDGAV GCVPLCSMDV RLPSPDCPFP
160 170 180 190 200
RRVKLPGKCC EEWVCDEPKD RTAVGPALAA YRLEDTFGPD PTMMRANCLV
210 220 230 240 250
QTTEWSACSK TCGMGISTRV TNDNTFCRLE KQSRLCMVRP CEADLEENIK
260 270 280 290 300
KGKKCIRTPK IAKPVKFELS GCTSVKTYRA KFCGVCTDGR CCTPHRTTTL
310 320 330 340
PVEFKCPDGE IMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BJW0 | H3BJW0_MOUSE | CCN family member 2 | Ccn2 Ctgf | 293 | Annotation score: | ||
H3BK14 | H3BK14_MOUSE | CCN family member 2 | Ccn2 Ctgf | 80 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | M → T in AAA37627 (PubMed:1888698).Curated | 1 | |
Sequence conflicti | 24 | M → T in AAA37628 (PubMed:1888698).Curated | 1 | |
Sequence conflicti | 24 | M → T in AAA73135 (PubMed:2029337).Curated | 1 | |
Sequence conflicti | 24 | M → T in AAH06783 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 161 | E → K in AAA37627 (PubMed:1888698).Curated | 1 | |
Sequence conflicti | 161 | E → K in AAA37628 (PubMed:1888698).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M70641 Genomic DNA Translation: AAA37627.1 M70642 mRNA Translation: AAA37628.1 M80263 mRNA Translation: AAA73135.1 AC099695 Genomic DNA No translation available. CH466540 Genomic DNA Translation: EDL04783.1 BC006783 mRNA Translation: AAH06783.1 |
CCDSi | CCDS23751.1 |
PIRi | A40578 |
RefSeqi | NP_034347.2, NM_010217.2 |
Genome annotation databases
Ensembli | ENSMUST00000020171; ENSMUSP00000020171; ENSMUSG00000019997 |
GeneIDi | 14219 |
KEGGi | mmu:14219 |
UCSCi | uc011xbr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M70641 Genomic DNA Translation: AAA37627.1 M70642 mRNA Translation: AAA37628.1 M80263 mRNA Translation: AAA73135.1 AC099695 Genomic DNA No translation available. CH466540 Genomic DNA Translation: EDL04783.1 BC006783 mRNA Translation: AAH06783.1 |
CCDSi | CCDS23751.1 |
PIRi | A40578 |
RefSeqi | NP_034347.2, NM_010217.2 |
3D structure databases
SMRi | P29268 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199679, 3 interactors |
IntActi | P29268, 1 interactor |
STRINGi | 10090.ENSMUSP00000020171 |
PTM databases
PhosphoSitePlusi | P29268 |
Proteomic databases
CPTACi | non-CPTAC-3782 |
MaxQBi | P29268 |
PaxDbi | P29268 |
PeptideAtlasi | P29268 |
PRIDEi | P29268 |
ProteomicsDBi | 284143 |
Protocols and materials databases
ABCDi | P29268, 1 sequenced antibody |
Antibodypediai | 3916, 927 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020171; ENSMUSP00000020171; ENSMUSG00000019997 |
GeneIDi | 14219 |
KEGGi | mmu:14219 |
UCSCi | uc011xbr.1, mouse |
Organism-specific databases
CTDi | 1490 |
MGIi | MGI:95537, Ccn2 |
Phylogenomic databases
eggNOGi | ENOG502QQDX, Eukaryota |
GeneTreei | ENSGT00940000155019 |
HOGENOMi | CLU_063247_1_0_1 |
InParanoidi | P29268 |
OMAi | QDCGGQC |
OrthoDBi | 999958at2759 |
TreeFami | TF326070 |
Miscellaneous databases
BioGRID-ORCSi | 14219, 0 hits in 52 CRISPR screens |
ChiTaRSi | Ccn2, mouse |
PROi | PR:P29268 |
RNActi | P29268, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000019997, Expressed in decidua and 363 other tissues |
Genevisiblei | P29268, MM |
Family and domain databases
Gene3Di | 2.20.100.10, 1 hit |
InterProi | View protein in InterPro IPR006207, Cys_knot_C IPR006208, Glyco_hormone_CN IPR009030, Growth_fac_rcpt_cys_sf IPR000867, IGFBP-like IPR012395, IGFBP_CNN IPR017891, Insulin_GF-bd_Cys-rich_CS IPR043973, TSP1_CCN IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf IPR001007, VWF_dom |
Pfami | View protein in Pfam PF00007, Cys_knot, 1 hit PF00219, IGFBP, 1 hit PF19035, TSP1_CCN, 1 hit PF00093, VWC, 1 hit |
PIRSFi | PIRSF036495, IGFBP_rP_CNN, 1 hit |
SMARTi | View protein in SMART SM00041, CT, 1 hit SM00121, IB, 1 hit SM00209, TSP1, 1 hit SM00214, VWC, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit SSF82895, SSF82895, 1 hit |
PROSITEi | View protein in PROSITE PS01185, CTCK_1, 1 hit PS01225, CTCK_2, 1 hit PS00222, IGFBP_N_1, 1 hit PS51323, IGFBP_N_2, 1 hit PS50092, TSP1, 1 hit PS01208, VWFC_1, 1 hit PS50184, VWFC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCN2_MOUSE | |
Accessioni | P29268Primary (citable) accession number: P29268 Secondary accession number(s): G5E830, Q922U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | October 3, 2012 | |
Last modified: | April 7, 2021 | |
This is version 175 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families