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UniProtKB - P29266 (3HIDH_RAT)
Protein
3-hydroxyisobutyrate dehydrogenase, mitochondrial
Gene
Hibadh
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalytic activityi
- EC:1.1.1.31
: L-valine degradation Pathwayi
This protein is involved in the pathway L-valine degradation, which is part of Amino-acid degradation.View all proteins of this organism that are known to be involved in the pathway L-valine degradation and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 107 | NADBy similarity | 1 | |
Binding sitei | 133 | NAD; via amide nitrogenBy similarity | 1 | |
Active sitei | 208 | Curated | 1 | |
Binding sitei | 283 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 39 – 68 | NADBy similarityAdd BLAST | 30 | |
Nucleotide bindingi | 102 – 103 | NADBy similarity | 2 |
GO - Molecular functioni
- 3-hydroxyisobutyrate dehydrogenase activity Source: UniProtKB
- NAD binding Source: InterPro
- NADP binding Source: InterPro
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: GO_Central
GO - Biological processi
- valine catabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Branched-chain amino acid catabolism |
Ligand | NAD |
Enzyme and pathway databases
Reactomei | R-RNO-70895, Branched-chain amino acid catabolism |
SABIO-RKi | P29266 |
UniPathwayi | UPA00362 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Hibadh |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708399, Hibadh |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrion Source: GO_Central
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 68 | D → R: Decrease of activity with NAD, increase of activity with NADP. 1 Publication | 1 | |
Mutagenesisi | 208 | K → A, H, N or R: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 212 | N → Q: Decrease in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 35 | MitochondrionBy similarityAdd BLAST | 35 | |
ChainiPRO_0000007160 | 36 – 335 | 3-hydroxyisobutyrate dehydrogenase, mitochondrialAdd BLAST | 300 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 59 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 59 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 75 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 75 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 94 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 120 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 140 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 144 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 148 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 148 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 237 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 237 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 241 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 241 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 296 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 320 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 320 | N6-succinyllysine; alternateBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | P29266 |
PRIDEi | P29266 |
PTM databases
iPTMneti | P29266 |
PhosphoSitePlusi | P29266 |
Expressioni
Tissue specificityi
Higher level in kidney, liver, and heart than in muscle.
Gene expression databases
Bgeei | ENSRNOG00000008063, Expressed in kidney and 21 other tissues |
Genevisiblei | P29266, RN |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
IntActi | P29266, 1 interactor |
STRINGi | 10116.ENSRNOP00000011069 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0409, Eukaryota |
GeneTreei | ENSGT00940000155255 |
HOGENOMi | CLU_035117_6_0_1 |
InParanoidi | P29266 |
OMAi | WSSEVNN |
OrthoDBi | 812358at2759 |
PhylomeDBi | P29266 |
TreeFami | TF314043 |
Family and domain databases
Gene3Di | 1.10.1040.10, 1 hit |
InterProi | View protein in InterPro IPR002204, 3-OH-isobutyrate_DH-rel_CS IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR006115, 6PGDH_NADP-bd IPR011548, HIBADH IPR015815, HIBADH-related IPR036291, NAD(P)-bd_dom_sf IPR029154, NADP-bd |
Pfami | View protein in Pfam PF14833, NAD_binding_11, 1 hit PF03446, NAD_binding_2, 1 hit |
PIRSFi | PIRSF000103, HIBADH, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01692, HIBADH, 1 hit |
PROSITEi | View protein in PROSITE PS00895, 3_HYDROXYISOBUT_DH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P29266-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAASLGFRGA ASGLRYWSGR RRPVGSLAAV CSRSMASKTP VGFIGLGNMG
60 70 80 90 100
NPMAKNLIKH GYPLILYDVF PDVCKEFKEA GEQVASSPAD VAEKADRIIT
110 120 130 140 150
MLPSSMNSIE VYSGANGILK KVKKGSLLID SSTIDPSVSK ELAKEVEKMG
160 170 180 190 200
AVFMDAPVSG GVGAARSGNL TFMVGGVENE FAAAQELLGC MGSNVLYCGA
210 220 230 240 250
VGSGQSAKIC NNMLLAISMI GTAEAMNLGI RSGLDPKLLA KILNMSSGRC
260 270 280 290 300
WSSDTYNPVP GVMDGVPSSN NYQGGFGTTL MAKDLGLAQD SATSTKTPIL
310 320 330
LGSVAHQIYR MMCSKGYSKK DFSSVFQYLR EEETF
Sequence cautioni
The sequence AAA50312 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04628 mRNA Translation: AAA50312.1 Frameshift. BC127442 mRNA Translation: AAI27443.1 |
PIRi | A32867 |
RefSeqi | NP_071579.1, NM_022243.1 |
Genome annotation databases
Ensembli | ENSRNOT00000011069; ENSRNOP00000011069; ENSRNOG00000008063 |
GeneIDi | 63938 |
KEGGi | rno:63938 |
UCSCi | RGD:708399, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04628 mRNA Translation: AAA50312.1 Frameshift. BC127442 mRNA Translation: AAI27443.1 |
PIRi | A32867 |
RefSeqi | NP_071579.1, NM_022243.1 |
3D structure databases
SMRi | P29266 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P29266, 1 interactor |
STRINGi | 10116.ENSRNOP00000011069 |
PTM databases
iPTMneti | P29266 |
PhosphoSitePlusi | P29266 |
Proteomic databases
PaxDbi | P29266 |
PRIDEi | P29266 |
Genome annotation databases
Ensembli | ENSRNOT00000011069; ENSRNOP00000011069; ENSRNOG00000008063 |
GeneIDi | 63938 |
KEGGi | rno:63938 |
UCSCi | RGD:708399, rat |
Organism-specific databases
CTDi | 11112 |
RGDi | 708399, Hibadh |
Phylogenomic databases
eggNOGi | KOG0409, Eukaryota |
GeneTreei | ENSGT00940000155255 |
HOGENOMi | CLU_035117_6_0_1 |
InParanoidi | P29266 |
OMAi | WSSEVNN |
OrthoDBi | 812358at2759 |
PhylomeDBi | P29266 |
TreeFami | TF314043 |
Enzyme and pathway databases
UniPathwayi | UPA00362 |
Reactomei | R-RNO-70895, Branched-chain amino acid catabolism |
SABIO-RKi | P29266 |
Miscellaneous databases
PROi | PR:P29266 |
Gene expression databases
Bgeei | ENSRNOG00000008063, Expressed in kidney and 21 other tissues |
Genevisiblei | P29266, RN |
Family and domain databases
Gene3Di | 1.10.1040.10, 1 hit |
InterProi | View protein in InterPro IPR002204, 3-OH-isobutyrate_DH-rel_CS IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR006115, 6PGDH_NADP-bd IPR011548, HIBADH IPR015815, HIBADH-related IPR036291, NAD(P)-bd_dom_sf IPR029154, NADP-bd |
Pfami | View protein in Pfam PF14833, NAD_binding_11, 1 hit PF03446, NAD_binding_2, 1 hit |
PIRSFi | PIRSF000103, HIBADH, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01692, HIBADH, 1 hit |
PROSITEi | View protein in PROSITE PS00895, 3_HYDROXYISOBUT_DH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 3HIDH_RAT | |
Accessioni | P29266Primary (citable) accession number: P29266 Secondary accession number(s): A1L107 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | January 24, 2001 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families