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Protein

Folic acid synthesis protein fol1

Gene

fol1

Organism
Pneumocystis carinii
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes three sequential steps of tetrahydrofolate biosynthesis.1 Publication

Catalytic activityi

7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde.3 Publications
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.3 Publications
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.3 Publications

Cofactori

Mg2+By similarity

Kineticsi

  1. KM=2.3 µM for 7,8-dihydroneopterin2 Publications
  2. KM=3.6 µM for 6-hydroxymethyl-7,8-dihydropterin2 Publications
  1. Vmax=36 nmol/min/mg enzyme for 7,8-dihydroneopterin2 Publications

pH dependencei

Optimum pH is 8.2 Publications

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 3 and 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Folic acid synthesis protein fol1 (fol1)
  4. Folic acid synthesis protein fol1 (fol1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Folic acid synthesis protein fol1 (fol1)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi478MagnesiumBy similarity1
Binding sitei5176-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei5526-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei5716-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei6436-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei6836-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Lyase, Multifunctional enzyme, Transferase
Biological processFolate biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKiP29251
UniPathwayi
UPA00077;UER00154

UPA00077;UER00155

UPA00077;UER00156

Names & Taxonomyi

Protein namesi
Recommended name:
Folic acid synthesis protein fol1
Including the following 3 domains:
Dihydroneopterin aldolase1 Publication (EC:4.1.2.252 Publications)
Short name:
DHNA1 Publication
Alternative name(s):
7,8-dihydroneopterin aldolase
FasA1 Publication
FasB1 Publication
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase1 Publication (EC:2.7.6.32 Publications)
Short name:
HPPK
Alternative name(s):
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
PPPK1 Publication
Dihydropterin pyrophosphokinase1 Publication
FasC1 Publication
Dihydropteroate synthase1 Publication (EC:2.5.1.151 Publication)
Short name:
DHPS1 Publication
Alternative name(s):
Dihydropteroate pyrophosphorylase
FasD1 Publication
Gene namesi
Name:fol1
Synonyms:fas1 Publication
OrganismiPneumocystis carinii
Taxonomic identifieri4754 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaPneumocystidomycetesPneumocystidaceaePneumocystis

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39D → E: Abolishes DHNA activity. 1 Publication1
Mutagenesisi53G → A: Reduces DHNA activity 11-fold. 1 Publication1
Mutagenesisi63Q → N: Reduces DHNA activity 16-fold. 1 Publication1
Mutagenesisi161D → E: No effect. 1 Publication1
Mutagenesisi175G → A: Abolishes DHNA activity. 1 Publication1
Mutagenesisi185Q → N: Reduces DHNA activity 24-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682421 – 740Folic acid synthesis protein fol1Add BLAST740

Structurei

3D structure databases

ProteinModelPortaliP29251
SMRiP29251
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini471 – 730Pterin-bindingPROSITE-ProRule annotationAdd BLAST260

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 160DHNA 1Add BLAST122
Regioni161 – 280DHNA 2Add BLAST120
Regioni291 – 449HPPKAdd BLAST159
Regioni473 – 740DHPSAdd BLAST268
Regioni718 – 7206-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Sequence similaritiesi

In the N-terminal section; belongs to the DHNA family.Curated
In the central section; belongs to the HPPK family.Curated
In the C-terminal section; belongs to the DHPS family.Curated

Family and domain databases

CDDicd00534 DHNA_DHNTPE, 2 hits
cd00739 DHPS, 1 hit
cd00483 HPPK, 1 hit
Gene3Di3.20.20.20, 1 hit
3.30.70.560, 1 hit
InterProiView protein in InterPro
IPR006390 DHP_synth
IPR011005 Dihydropteroate_synth-like
IPR006157 FolB_dom
IPR016261 Folic_acid_synth
IPR000550 Hppk
IPR035907 Hppk_sf
IPR000489 Pterin-binding_dom
PfamiView protein in Pfam
PF02152 FolB, 2 hits
PF01288 HPPK, 1 hit
PF00809 Pterin_bind, 1 hit
PIRSFiPIRSF000741 Folic_acid_synth, 1 hit
SMARTiView protein in SMART
SM00905 FolB, 2 hits
SUPFAMiSSF51717 SSF51717, 1 hit
SSF55083 SSF55083, 1 hit
TIGRFAMsiTIGR01496 DHPS, 1 hit
TIGR00526 folB_dom, 2 hits
TIGR01498 folK, 1 hit
PROSITEiView protein in PROSITE
PS00792 DHPS_1, 1 hit
PS00793 DHPS_2, 1 hit
PS00794 HPPK, 1 hit
PS50972 PTERIN_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P29251-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIFKSLKIFP FYQIYGFRFL KGMIFKKKIH LSKLNKNHDL IHIHSLTLKS
60 70 80 90 100
IVGKNSWAQR LLQPVVLTLS MGINASLSGN MDDLSYSIDY ATVYKEVFKL
110 120 130 140 150
VENSKFENLL DLSDKISKVV LGDKCKGNWV KVIAETPKGH LLAETGLQII
160 170 180 190 200
RRKDGIREID DQFFIKNLSL YTIIGINPEE RVNKQNIIID LILFKSSINL
210 220 230 240 250
ECKDDFIINT YNIEKLLKEI VKHVEESTFK TIEALALSIA RISCISHNIE
260 270 280 290 300
KIIVKVKKSC ALAFAESAGV EIVRSRSCFS SNNYIKSENS IDNEAVYISL
310 320 330 340 350
GSNLGNRIKF ILDAIEKMSI KGIKVLKTSM LYESKPMYFK DQPAFYNAVC
360 370 380 390 400
KVQTSLHPEQ LLFELQLIEK ELGRVKVIDK GPRCIDLDIV FYGRKIINSE
410 420 430 440 450
SLIIPHPRVL ERSFVLKPLL DISGDLVHPV TGLSIASYFE KIVDHDIKPV
460 470 480 490 500
LPFLYKNKSI DFSFRSYKAP TYIMAILNLT PDSFFDGGIH SYDSVLIDVE
510 520 530 540 550
KFINAGATII DIGGQSTRPG SYIIPLEEEI FRVIPAIKYL QKTYPDILIS
560 570 580 590 600
IDTFRSEVAE QAVKAGASLV NDISGGRYDP KMFNTVARLK VPICIMHMRG
610 620 630 640 650
NFLNMDNLTD YGTDIIEQIT IELEKLLNSA EKSGIPRWNI ILDPGLGFSK
660 670 680 690 700
TLHQNIELLR RFNELKSKNC FNGLPWLLGP SRKRFTGFIT GDNMPKDRIW
710 720 730 740
GTVAAVVASI SGGCDIIRVH DVYEMYKISK MSDAIWKEIY
Length:740
Mass (Da):83,962
Last modified:December 1, 1992 - v1
Checksum:i328F6EB91B4499EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86602 Genomic DNA Translation: AAA33790.1
PIRiS28666

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86602 Genomic DNA Translation: AAA33790.1
PIRiS28666

3D structure databases

ProteinModelPortaliP29251
SMRiP29251
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00077;UER00154

UPA00077;UER00155

UPA00077;UER00156

SABIO-RKiP29251

Family and domain databases

CDDicd00534 DHNA_DHNTPE, 2 hits
cd00739 DHPS, 1 hit
cd00483 HPPK, 1 hit
Gene3Di3.20.20.20, 1 hit
3.30.70.560, 1 hit
InterProiView protein in InterPro
IPR006390 DHP_synth
IPR011005 Dihydropteroate_synth-like
IPR006157 FolB_dom
IPR016261 Folic_acid_synth
IPR000550 Hppk
IPR035907 Hppk_sf
IPR000489 Pterin-binding_dom
PfamiView protein in Pfam
PF02152 FolB, 2 hits
PF01288 HPPK, 1 hit
PF00809 Pterin_bind, 1 hit
PIRSFiPIRSF000741 Folic_acid_synth, 1 hit
SMARTiView protein in SMART
SM00905 FolB, 2 hits
SUPFAMiSSF51717 SSF51717, 1 hit
SSF55083 SSF55083, 1 hit
TIGRFAMsiTIGR01496 DHPS, 1 hit
TIGR00526 folB_dom, 2 hits
TIGR01498 folK, 1 hit
PROSITEiView protein in PROSITE
PS00792 DHPS_1, 1 hit
PS00793 DHPS_2, 1 hit
PS00794 HPPK, 1 hit
PS50972 PTERIN_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFOL1_PNECA
AccessioniPrimary (citable) accession number: P29251
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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