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UniProtKB - P29251 (FOL1_PNECA)
Protein
Folic acid synthesis protein fol1
Gene
fol1
Organism
Pneumocystis carinii
Status
Functioni
Catalyzes three sequential steps of tetrahydrofolate biosynthesis.
1 PublicationCatalytic activityi
- EC:4.1.2.253 Publications
- 6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H+3 PublicationsEC:2.7.6.33 Publications
- (7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate3 PublicationsEC:2.5.1.153 Publications
Cofactori
Mg2+By similarity
Kineticsi
- KM=2.3 µM for 7,8-dihydroneopterin2 Publications
- KM=3.6 µM for 6-hydroxymethyl-7,8-dihydropterin2 Publications
- Vmax=36 nmol/min/mg enzyme for 7,8-dihydroneopterin2 Publications
pH dependencei
Optimum pH is 8.2 Publications
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 3 and 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate. This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Pathwayi: tetrahydrofolate biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate. This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 478 | MagnesiumBy similarity | 1 | |
Binding sitei | 517 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 552 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 571 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 643 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 683 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 |
GO - Molecular functioni
- 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- dihydroneopterin aldolase activity Source: UniProtKB-EC
- dihydropteroate synthase activity Source: UniProtKB-EC
- kinase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- folic acid biosynthetic process Source: UniProtKB-KW
- tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Kinase, Lyase, Multifunctional enzyme, Transferase |
Biological process | Folate biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
SABIO-RKi | P29251 |
UniPathwayi | UPA00077;UER00154 UPA00077;UER00155 UPA00077;UER00156 |
Names & Taxonomyi
Protein namesi | Recommended name: Folic acid synthesis protein fol1Including the following 3 domains: Alternative name(s): 7,8-dihydroneopterin aldolase FasA1 Publication FasB1 Publication 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase1 Publication (EC:2.7.6.32 Publications) Short name: HPPK Alternative name(s): 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase Short name: PPPK1 Publication Dihydropterin pyrophosphokinase1 Publication FasC1 Publication Alternative name(s): Dihydropteroate pyrophosphorylase FasD1 Publication |
Gene namesi | Name:fol1 Synonyms:fas1 Publication |
Organismi | Pneumocystis carinii |
Taxonomic identifieri | 4754 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Pneumocystidomycetes › Pneumocystidaceae › Pneumocystis |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 39 | D → E: Abolishes DHNA activity. 1 Publication | 1 | |
Mutagenesisi | 53 | G → A: Reduces DHNA activity 11-fold. 1 Publication | 1 | |
Mutagenesisi | 63 | Q → N: Reduces DHNA activity 16-fold. 1 Publication | 1 | |
Mutagenesisi | 161 | D → E: No effect. 1 Publication | 1 | |
Mutagenesisi | 175 | G → A: Abolishes DHNA activity. 1 Publication | 1 | |
Mutagenesisi | 185 | Q → N: Reduces DHNA activity 24-fold. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168242 | 1 – 740 | Folic acid synthesis protein fol1Add BLAST | 740 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 471 – 730 | Pterin-bindingPROSITE-ProRule annotationAdd BLAST | 260 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 39 – 160 | DHNA 1Add BLAST | 122 | |
Regioni | 161 – 280 | DHNA 2Add BLAST | 120 | |
Regioni | 291 – 449 | HPPKAdd BLAST | 159 | |
Regioni | 473 – 740 | DHPSAdd BLAST | 268 | |
Regioni | 718 – 720 | 6-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity | 3 |
Sequence similaritiesi
In the N-terminal section; belongs to the DHNA family.Curated
In the central section; belongs to the HPPK family.Curated
In the C-terminal section; belongs to the DHPS family.Curated
Family and domain databases
CDDi | cd00534, DHNA_DHNTPE, 2 hits cd00739, DHPS, 1 hit cd00483, HPPK, 1 hit |
Gene3Di | 3.20.20.20, 1 hit 3.30.1130.10, 2 hits 3.30.70.560, 1 hit |
InterProi | View protein in InterPro IPR045031, DHP_synth IPR006390, DHP_synth_dom IPR011005, Dihydropteroate_synth-like IPR006157, FolB_dom IPR016261, Folic_acid_synth IPR043133, GTP-CH-I_C/QueF IPR000550, Hppk IPR035907, Hppk_sf IPR000489, Pterin-binding_dom |
PANTHERi | PTHR20941, PTHR20941, 1 hit |
Pfami | View protein in Pfam PF02152, FolB, 2 hits PF01288, HPPK, 1 hit PF00809, Pterin_bind, 1 hit |
PIRSFi | PIRSF000741, Folic_acid_synth, 1 hit |
SMARTi | View protein in SMART SM00905, FolB, 2 hits |
SUPFAMi | SSF51717, SSF51717, 1 hit SSF55083, SSF55083, 1 hit |
TIGRFAMsi | TIGR01496, DHPS, 1 hit TIGR00526, folB_dom, 2 hits TIGR01498, folK, 1 hit |
PROSITEi | View protein in PROSITE PS00792, DHPS_1, 1 hit PS00793, DHPS_2, 1 hit PS00794, HPPK, 1 hit PS50972, PTERIN_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P29251-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIFKSLKIFP FYQIYGFRFL KGMIFKKKIH LSKLNKNHDL IHIHSLTLKS
60 70 80 90 100
IVGKNSWAQR LLQPVVLTLS MGINASLSGN MDDLSYSIDY ATVYKEVFKL
110 120 130 140 150
VENSKFENLL DLSDKISKVV LGDKCKGNWV KVIAETPKGH LLAETGLQII
160 170 180 190 200
RRKDGIREID DQFFIKNLSL YTIIGINPEE RVNKQNIIID LILFKSSINL
210 220 230 240 250
ECKDDFIINT YNIEKLLKEI VKHVEESTFK TIEALALSIA RISCISHNIE
260 270 280 290 300
KIIVKVKKSC ALAFAESAGV EIVRSRSCFS SNNYIKSENS IDNEAVYISL
310 320 330 340 350
GSNLGNRIKF ILDAIEKMSI KGIKVLKTSM LYESKPMYFK DQPAFYNAVC
360 370 380 390 400
KVQTSLHPEQ LLFELQLIEK ELGRVKVIDK GPRCIDLDIV FYGRKIINSE
410 420 430 440 450
SLIIPHPRVL ERSFVLKPLL DISGDLVHPV TGLSIASYFE KIVDHDIKPV
460 470 480 490 500
LPFLYKNKSI DFSFRSYKAP TYIMAILNLT PDSFFDGGIH SYDSVLIDVE
510 520 530 540 550
KFINAGATII DIGGQSTRPG SYIIPLEEEI FRVIPAIKYL QKTYPDILIS
560 570 580 590 600
IDTFRSEVAE QAVKAGASLV NDISGGRYDP KMFNTVARLK VPICIMHMRG
610 620 630 640 650
NFLNMDNLTD YGTDIIEQIT IELEKLLNSA EKSGIPRWNI ILDPGLGFSK
660 670 680 690 700
TLHQNIELLR RFNELKSKNC FNGLPWLLGP SRKRFTGFIT GDNMPKDRIW
710 720 730 740
GTVAAVVASI SGGCDIIRVH DVYEMYKISK MSDAIWKEIY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86602 Genomic DNA Translation: AAA33790.1 |
PIRi | S28666 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86602 Genomic DNA Translation: AAA33790.1 |
PIRi | S28666 |
3D structure databases
AlphaFoldDBi | P29251 |
SMRi | P29251 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00154 UPA00077;UER00155 UPA00077;UER00156 |
SABIO-RKi | P29251 |
Family and domain databases
CDDi | cd00534, DHNA_DHNTPE, 2 hits cd00739, DHPS, 1 hit cd00483, HPPK, 1 hit |
Gene3Di | 3.20.20.20, 1 hit 3.30.1130.10, 2 hits 3.30.70.560, 1 hit |
InterProi | View protein in InterPro IPR045031, DHP_synth IPR006390, DHP_synth_dom IPR011005, Dihydropteroate_synth-like IPR006157, FolB_dom IPR016261, Folic_acid_synth IPR043133, GTP-CH-I_C/QueF IPR000550, Hppk IPR035907, Hppk_sf IPR000489, Pterin-binding_dom |
PANTHERi | PTHR20941, PTHR20941, 1 hit |
Pfami | View protein in Pfam PF02152, FolB, 2 hits PF01288, HPPK, 1 hit PF00809, Pterin_bind, 1 hit |
PIRSFi | PIRSF000741, Folic_acid_synth, 1 hit |
SMARTi | View protein in SMART SM00905, FolB, 2 hits |
SUPFAMi | SSF51717, SSF51717, 1 hit SSF55083, SSF55083, 1 hit |
TIGRFAMsi | TIGR01496, DHPS, 1 hit TIGR00526, folB_dom, 2 hits TIGR01498, folK, 1 hit |
PROSITEi | View protein in PROSITE PS00792, DHPS_1, 1 hit PS00793, DHPS_2, 1 hit PS00794, HPPK, 1 hit PS50972, PTERIN_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FOL1_PNECA | |
Accessioni | P29251Primary (citable) accession number: P29251 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families