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Protein

Gag polyprotein

Gene

gag

Organism
FBR murine osteosarcoma virus (FBR-MSV) (Finkel-Biskis-Reilly murine osteosarcoma virus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity

Miscellaneous

This protein is synthesized as a Gag-Fos-Fox polyprotein.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 3 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Gene namesi
Name:gag
OrganismiFBR murine osteosarcoma virus (FBR-MSV) (Finkel-Biskis-Reilly murine osteosarcoma virus)
Taxonomic identifieri353765 [NCBI]
Taxonomic lineageiVirusesOrterviralesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000237129 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00003908172 – 310Gag polyproteinAdd BLAST309
ChainiPRO_00000409462 – 129Matrix protein p15Sequence analysisAdd BLAST128
ChainiPRO_0000040947130 – 214RNA-binding phosphoprotein p12Sequence analysisAdd BLAST85
ChainiPRO_0000040948215 – 310Capsid protein p30Sequence analysisAdd BLAST96

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei129 – 130Cleavage; by viral proteaseBy similarity2
Sitei214 – 215Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Protein-protein interaction databases

ELMiP29175

Structurei

3D structure databases

ProteinModelPortaliP29175
SMRiP29175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi109 – 112PTAP/PSAP motif4
Motifi128 – 132LYPX(n)L motif5
Motifi161 – 164PPXY motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L which potentially interacts with PDCD6IP (By similarity).By similarity

Family and domain databases

Gene3Di1.10.150.180, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR000840 G_retro_matrix
IPR036946 G_retro_matrix_sf
IPR002079 Gag_p12
IPR003036 Gag_P30
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
PfamiView protein in Pfam
PF01140 Gag_MA, 1 hit
PF01141 Gag_p12, 1 hit
PF02093 Gag_p30, 1 hit
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29175-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGQTVTTPLS LTLEHWGDVQ RIASNQSVDV KKRRWVTFCS AEWPTFDVGW
60 70 80 90 100
PQDGTFNLDI ILQVKSKVFS PGPHGHPDQV PYIVTWEAIA YEPPPWVKPF
110 120 130 140 150
VSPKLSPSPT APILPSGPST QPPPRSALYP ALTPSIKPRP SKPQVLSDDG
160 170 180 190 200
GPLIDLLTED PPPYGEQGPS SSDGDGDREE ATSTSEIPAP SPMVSRLRGK
210 220 230 240 250
RDPPAADSTT SRAFPLRLGG NGQKNNNPSF SEDPGKLTAL IESVLTTHQP
260 270 280 290 300
TWDDCQQLLG TLLTGEEKQR VLLEARKAVR GNDGRPTQMP NEVNAAFPLE
310
RPDWDYTTPE
Length:310
Mass (Da):33,849
Last modified:January 23, 2007 - v3
Checksum:iF5713E16959C5F8C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02712 Genomic DNA Translation: AAA46573.1 Different termination.
PIRiA23244 FOMVFB

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02712 Genomic DNA Translation: AAA46573.1 Different termination.
PIRiA23244 FOMVFB

3D structure databases

ProteinModelPortaliP29175
SMRiP29175
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiP29175

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.150.180, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR000840 G_retro_matrix
IPR036946 G_retro_matrix_sf
IPR002079 Gag_p12
IPR003036 Gag_P30
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
PfamiView protein in Pfam
PF01140 Gag_MA, 1 hit
PF01141 Gag_p12, 1 hit
PF02093 Gag_p30, 1 hit
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGAG_MSVFR
AccessioniPrimary (citable) accession number: P29175
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 92 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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